Click here to switch to the map view.

The map label for this gene is nylA [H]

Identifier: 197105736

GI number: 197105736

Start: 2576401

End: 2577822

Strand: Direct

Name: nylA [H]

Synonym: PHZ_c2274

Alternate gene names: 197105736

Gene position: 2576401-2577822 (Clockwise)

Preceding gene: 197105735

Following gene: 197105737

Centisome position: 64.47

GC content: 72.15

Gene sequence:

>1422_bases
ATGGACGACTATTCCGACTACGACGGCCTGGGCCTGGCCGAGCTGGTCCGCACGAAGCAGGTGACGCCTGCCGAGCTGGT
CGAGGCCGCCATCGTCCGCATCGAGCGGCACAACCCGACTCTGAACGCGGTCGTCCACAAGGGCTACGACGACGCCCGCC
GCTGGGCCGCGTCGGACCTGCCGGACGGCCCCTTCCGGGGCGTGCCGTTCCTGATCAAGGACCTGGGCGCCCAGGTGAAG
GGCTGGCCGCGCACCTCGGGCAGCCGCTTCGCCCAGGTCCCCGCCGACGCCGAGGACAGCGAGCTTGTCCGCCGTTACCG
CGACGCCGGCGTGGTGCTGCTGGGCAAGACCAACACGCCCGAGTTCGGCATCCCGGGCGTGACGCATTCCGAACAGCTCG
GCTCGTGCCGCAATCCCTGGAACCCGGACCACATCTCGGGCGGCTCCTCGGGCGGGGCGGCCTCGGCCACGGCGGCCGGG
ATCGTGCCCCTGGCGCACGCCAGCGACGGCCTGGGCTCGATCCGCATCCCCGCCGCCTGCTGCGGCCTGTTCGGCCTGAA
GGTCACCCGCGACCGCACGCCGATCTGCGAGGTCTCGGACGGGGCCCTCGGCTTCTCGGTGCACCACGTGGTCAGCCGCA
CGGTGCGCGACAGCGCCGCCATGCTGGACGCCACCGGCTATCCCCAGGCCGCCGATCCGTTCCAGGCTCCGGCCAAGGGC
GGCCCCTACCTCGCCGAGCTTGAGAAGAGCCCGGGGCGCCTGCGGATCGCCTGGTCCTCTGAGACGCCCAGCGGCCGGCC
CATTCCCGACGAGATGATGGACGCCCTGCTGCGCACCGTCGAACTGCTCGACGGCCTCGGCCACGAGGTGATCGAGCGGG
GCCTTGGGATCGACTACCGCCTGCTCTACCGCGCCCAGGGCCTGGCCAGCGCCTCCAACTTCTCGGCCAATGTCCAGCGC
TGGGTGGAGGCGCTCGGCCGCGAGCCGGGCGACGACGAGCTCGGCCCCCTGGCCCGCCGCGGCTACGAGGCCGGCAAGCG
GCTCACCGGCCAGCAGGCGTTCTGGGGCTTCCAGCAGCTGCGGCTGATGAACCGCCAGATCCTCGCCGCCTTCGAGGACA
TCGACGTCTTCCTCTCGCCGGTGATGGCCACCCCCGCGCCGCGCGCCGACTGGCTTGACCCGCTGATGGACGACCTGAAG
GAGTTCGACCGCCGGCAGGCGGCGACCTATCCCTACACCCCGCCGTTCAACTTCACCGGCCAGCCGTCGATGTCGGTCCC
CCTCTGGGAGTCCGAGGCCGGCCTGCCGATCGGGATGATGTTCAGCGGCCGCTTCGCCGACGAGGCGACCCTGTTCCGCC
TCGCCGCCCAGCTCGAGAAGGAGGCCCCGTGGAAGGACCGCCGCCCGCAGGTGTCGGGCTGA

Upstream 100 bases:

>100_bases
CGCTCGACGCGGCCGAGGACGCGTTCGCCGCCCTGAAGCGCCAGGCGGGCGCCCTGCCGCCCGTCGAGAAACTCGCCTTC
CTCACCGCCGGAGCCCATTG

Downstream 100 bases:

>100_bases
TGCCGGCCGCGCTCGCCCTCGCCGCCCTGCTGGGGCTGGCCTGCGTCGGTTTCGGGGCCTTCGGGGCGCACGGGGTCGCC
GACCCCAAGGCCCAGGAGTG

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MDDYSDYDGLGLAELVRTKQVTPAELVEAAIVRIERHNPTLNAVVHKGYDDARRWAASDLPDGPFRGVPFLIKDLGAQVK
GWPRTSGSRFAQVPADAEDSELVRRYRDAGVVLLGKTNTPEFGIPGVTHSEQLGSCRNPWNPDHISGGSSGGAASATAAG
IVPLAHASDGLGSIRIPAACCGLFGLKVTRDRTPICEVSDGALGFSVHHVVSRTVRDSAAMLDATGYPQAADPFQAPAKG
GPYLAELEKSPGRLRIAWSSETPSGRPIPDEMMDALLRTVELLDGLGHEVIERGLGIDYRLLYRAQGLASASNFSANVQR
WVEALGREPGDDELGPLARRGYEAGKRLTGQQAFWGFQQLRLMNRQILAAFEDIDVFLSPVMATPAPRADWLDPLMDDLK
EFDRRQAATYPYTPPFNFTGQPSMSVPLWESEAGLPIGMMFSGRFADEATLFRLAAQLEKEAPWKDRRPQVSG

Sequences:

>Translated_473_residues
MDDYSDYDGLGLAELVRTKQVTPAELVEAAIVRIERHNPTLNAVVHKGYDDARRWAASDLPDGPFRGVPFLIKDLGAQVK
GWPRTSGSRFAQVPADAEDSELVRRYRDAGVVLLGKTNTPEFGIPGVTHSEQLGSCRNPWNPDHISGGSSGGAASATAAG
IVPLAHASDGLGSIRIPAACCGLFGLKVTRDRTPICEVSDGALGFSVHHVVSRTVRDSAAMLDATGYPQAADPFQAPAKG
GPYLAELEKSPGRLRIAWSSETPSGRPIPDEMMDALLRTVELLDGLGHEVIERGLGIDYRLLYRAQGLASASNFSANVQR
WVEALGREPGDDELGPLARRGYEAGKRLTGQQAFWGFQQLRLMNRQILAAFEDIDVFLSPVMATPAPRADWLDPLMDDLK
EFDRRQAATYPYTPPFNFTGQPSMSVPLWESEAGLPIGMMFSGRFADEATLFRLAAQLEKEAPWKDRRPQVSG
>Mature_473_residues
MDDYSDYDGLGLAELVRTKQVTPAELVEAAIVRIERHNPTLNAVVHKGYDDARRWAASDLPDGPFRGVPFLIKDLGAQVK
GWPRTSGSRFAQVPADAEDSELVRRYRDAGVVLLGKTNTPEFGIPGVTHSEQLGSCRNPWNPDHISGGSSGGAASATAAG
IVPLAHASDGLGSIRIPAACCGLFGLKVTRDRTPICEVSDGALGFSVHHVVSRTVRDSAAMLDATGYPQAADPFQAPAKG
GPYLAELEKSPGRLRIAWSSETPSGRPIPDEMMDALLRTVELLDGLGHEVIERGLGIDYRLLYRAQGLASASNFSANVQR
WVEALGREPGDDELGPLARRGYEAGKRLTGQQAFWGFQQLRLMNRQILAAFEDIDVFLSPVMATPAPRADWLDPLMDDLK
EFDRRQAATYPYTPPFNFTGQPSMSVPLWESEAGLPIGMMFSGRFADEATLFRLAAQLEKEAPWKDRRPQVSG

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=194, Percent_Identity=32.4742268041237, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=25.6360078277886, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=306, Percent_Identity=27.7777777777778, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=349, Percent_Identity=27.2206303724928, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32565337, Length=176, Percent_Identity=32.9545454545455, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=308, Percent_Identity=26.2987012987013, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=308, Percent_Identity=26.2987012987013, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=397, Percent_Identity=27.2040302267003, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=25.0501002004008, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356731, Length=191, Percent_Identity=34.5549738219895, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550774, Length=192, Percent_Identity=31.7708333333333, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648435, Length=192, Percent_Identity=31.7708333333333, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648437, Length=192, Percent_Identity=31.7708333333333, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=24.6435845213849, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=26.0692464358452, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652985, Length=193, Percent_Identity=32.1243523316062, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19922090, Length=193, Percent_Identity=32.1243523316062, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24652981, Length=193, Percent_Identity=32.1243523316062, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24652983, Length=193, Percent_Identity=32.1243523316062, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648441, Length=126, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648439, Length=126, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51423; Mature: 51423

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDYSDYDGLGLAELVRTKQVTPAELVEAAIVRIERHNPTLNAVVHKGYDDARRWAASDL
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHEECCCHHHHHHHHCCC
PDGPFRGVPFLIKDLGAQVKGWPRTSGSRFAQVPADAEDSELVRRYRDAGVVLLGKTNTP
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCC
EFGIPGVTHSEQLGSCRNPWNPDHISGGSSGGAASATAAGIVPLAHASDGLGSIRIPAAC
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCEEHHHHH
CGLFGLKVTRDRTPICEVSDGALGFSVHHVVSRTVRDSAAMLDATGYPQAADPFQAPAKG
HHHHCCEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GPYLAELEKSPGRLRIAWSSETPSGRPIPDEMMDALLRTVELLDGLGHEVIERGLGIDYR
CCCHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEE
LLYRAQGLASASNFSANVQRWVEALGREPGDDELGPLARRGYEAGKRLTGQQAFWGFQQL
EHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RLMNRQILAAFEDIDVFLSPVMATPAPRADWLDPLMDDLKEFDRRQAATYPYTPPFNFTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QPSMSVPLWESEAGLPIGMMFSGRFADEATLFRLAAQLEKEAPWKDRRPQVSG
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDDYSDYDGLGLAELVRTKQVTPAELVEAAIVRIERHNPTLNAVVHKGYDDARRWAASDL
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHEECCCHHHHHHHHCCC
PDGPFRGVPFLIKDLGAQVKGWPRTSGSRFAQVPADAEDSELVRRYRDAGVVLLGKTNTP
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCC
EFGIPGVTHSEQLGSCRNPWNPDHISGGSSGGAASATAAGIVPLAHASDGLGSIRIPAAC
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCEEHHHHH
CGLFGLKVTRDRTPICEVSDGALGFSVHHVVSRTVRDSAAMLDATGYPQAADPFQAPAKG
HHHHCCEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GPYLAELEKSPGRLRIAWSSETPSGRPIPDEMMDALLRTVELLDGLGHEVIERGLGIDYR
CCCHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEE
LLYRAQGLASASNFSANVQRWVEALGREPGDDELGPLARRGYEAGKRLTGQQAFWGFQQL
EHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RLMNRQILAAFEDIDVFLSPVMATPAPRADWLDPLMDDLKEFDRRQAATYPYTPPFNFTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QPSMSVPLWESEAGLPIGMMFSGRFADEATLFRLAAQLEKEAPWKDRRPQVSG
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]