| Definition | Phenylobacterium zucineum HLK1, complete genome. |
|---|---|
| Accession | NC_011144 |
| Length | 3,996,255 |
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The map label for this gene is gra-orf6 [H]
Identifier: 197105691
GI number: 197105691
Start: 2520494
End: 2521297
Strand: Direct
Name: gra-orf6 [H]
Synonym: PHZ_c2229
Alternate gene names: 197105691
Gene position: 2520494-2521297 (Clockwise)
Preceding gene: 197105689
Following gene: 197105692
Centisome position: 63.07
GC content: 74.38
Gene sequence:
>804_bases ATGGCGGCCGGCGGGGACTTCCAGGACTTCGTGGTGGTGGTGACCGGCGCCTCGACCGGGCTCGGCCGGGCGATCGCCGT CGAGGTGGCGCGCCGCGGCGCCAAGGCCGTGGTGGTCAACTACGCGCGCAGCGCCAGCGAGGCCGAGGAGACCGCCGCCC TCGTGCGCGCCGAGGGCGCCGAGGGGGTGCTGGTGCAGGGCGACGTCGCCTCGGACGAGGATTGCCGCAAGATCGCCGCC GCCGCCCAGCCGTTCGGCCGCATCGACGCCCTGTTCAACAATGCGGGCACGACCCGCTTCGCCGCCCACGGCGACCTCGA CGCGGTCTCGGCGCAGGACTTCCTCGACCTCTACGCCGTCAACGTGGTGGGCGCCTACCAGATGGTCCGCGCGTGCCGGA GCCTGCTCGAGGCCGCCCCCCGCCACGGCGCGGTCGTGAACACGGCCTCCATCGCCGGCGTCACCGGGATCGGCTCCTCG ATCCCCTACGCCGCCTCCAAGGGCGCCATGACCACCATGACCCTGTCGCTGGCCCGGGCCCTGGCCCCGAAGATCCGCGT GAACGCCGTCTGCCCCGGCTTCATCGACACGCCCTGGTTCGGCAAGGGCATGCCGGACGCCGCCGTCGACCGCGTGCGGG CGAACGCCGAGGCCTCGACGCCGCTGCGGGCGGCCTCCACCCCCCAGGATATCGCCGAGGCGACGGTGTTCCTGGCCGCG CCGGCCTCGCGCCACGTCACCGGCGAAACCCTGCTGGTGGACGCCGGCATGCACCTCGGCTACGCGCCGACCGTGGCGCG CTAA
Upstream 100 bases:
>100_bases CCCCGCACGTCGCGGCCGAGCTGGCTCAGGTGGGTCTCTTCCATGGGCGCGCCTTTACCCGCTACTAGGGGCGCTAACAA CACACGCAAGGGAGAGCGAC
Downstream 100 bases:
>100_bases GCAGGGTGCGGTGCAGGCTGCGGGCGGGTTCTGCCCAATTTCCCGGCGCCGGCCACGTTGGGCGCACAAAGTTCGCTCAG CCCCTGCCGAGCTGCGCCAT
Product: short-chain dehydrogenase/reductase SDR
Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]
Alternate protein names: ORF6 [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MAAGGDFQDFVVVVTGASTGLGRAIAVEVARRGAKAVVVNYARSASEAEETAALVRAEGAEGVLVQGDVASDEDCRKIAA AAQPFGRIDALFNNAGTTRFAAHGDLDAVSAQDFLDLYAVNVVGAYQMVRACRSLLEAAPRHGAVVNTASIAGVTGIGSS IPYAASKGAMTTMTLSLARALAPKIRVNAVCPGFIDTPWFGKGMPDAAVDRVRANAEASTPLRAASTPQDIAEATVFLAA PASRHVTGETLLVDAGMHLGYAPTVAR
Sequences:
>Translated_267_residues MAAGGDFQDFVVVVTGASTGLGRAIAVEVARRGAKAVVVNYARSASEAEETAALVRAEGAEGVLVQGDVASDEDCRKIAA AAQPFGRIDALFNNAGTTRFAAHGDLDAVSAQDFLDLYAVNVVGAYQMVRACRSLLEAAPRHGAVVNTASIAGVTGIGSS IPYAASKGAMTTMTLSLARALAPKIRVNAVCPGFIDTPWFGKGMPDAAVDRVRANAEASTPLRAASTPQDIAEATVFLAA PASRHVTGETLLVDAGMHLGYAPTVAR >Mature_266_residues AAGGDFQDFVVVVTGASTGLGRAIAVEVARRGAKAVVVNYARSASEAEETAALVRAEGAEGVLVQGDVASDEDCRKIAAA AQPFGRIDALFNNAGTTRFAAHGDLDAVSAQDFLDLYAVNVVGAYQMVRACRSLLEAAPRHGAVVNTASIAGVTGIGSSI PYAASKGAMTTMTLSLARALAPKIRVNAVCPGFIDTPWFGKGMPDAAVDRVRANAEASTPLRAASTPQDIAEATVFLAAP ASRHVTGETLLVDAGMHLGYAPTVAR
Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI59889578, Length=240, Percent_Identity=32.9166666666667, Blast_Score=98, Evalue=7e-21, Organism=Homo sapiens, GI40254992, Length=254, Percent_Identity=31.1023622047244, Blast_Score=96, Evalue=2e-20, Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=30.8880308880309, Blast_Score=93, Evalue=2e-19, Organism=Homo sapiens, GI4758504, Length=203, Percent_Identity=32.0197044334975, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI32483357, Length=254, Percent_Identity=30.3149606299213, Blast_Score=82, Evalue=6e-16, Organism=Homo sapiens, GI109715829, Length=247, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=3e-15, Organism=Homo sapiens, GI5031737, Length=252, Percent_Identity=30.5555555555556, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=29.6875, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI83715985, Length=185, Percent_Identity=31.3513513513514, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI19923817, Length=270, Percent_Identity=24.8148148148148, Blast_Score=79, Evalue=6e-15, Organism=Homo sapiens, GI210032110, Length=193, Percent_Identity=29.5336787564767, Blast_Score=76, Evalue=4e-14, Organism=Homo sapiens, GI31542939, Length=192, Percent_Identity=29.6875, Blast_Score=75, Evalue=5e-14, Organism=Homo sapiens, GI4504505, Length=198, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=1e-12, Organism=Homo sapiens, GI7706318, Length=197, Percent_Identity=31.4720812182741, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI33667109, Length=196, Percent_Identity=32.6530612244898, Blast_Score=70, Evalue=3e-12, Organism=Homo sapiens, GI142976729, Length=194, Percent_Identity=29.3814432989691, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI7657478, Length=194, Percent_Identity=31.9587628865979, Blast_Score=65, Evalue=7e-11, Organism=Homo sapiens, GI10190704, Length=262, Percent_Identity=29.3893129770992, Blast_Score=65, Evalue=7e-11, Organism=Escherichia coli, GI1787335, Length=257, Percent_Identity=36.1867704280156, Blast_Score=122, Evalue=3e-29, Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=5e-24, Organism=Escherichia coli, GI1789378, Length=258, Percent_Identity=28.2945736434109, Blast_Score=96, Evalue=2e-21, Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=30.5555555555556, Blast_Score=96, Evalue=2e-21, Organism=Escherichia coli, GI87082160, Length=251, Percent_Identity=28.2868525896414, Blast_Score=93, Evalue=2e-20, Organism=Escherichia coli, GI1786812, Length=265, Percent_Identity=32.8301886792453, Blast_Score=92, Evalue=3e-20, Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=29.3172690763052, Blast_Score=88, Evalue=7e-19, Organism=Escherichia coli, GI1789208, Length=261, Percent_Identity=30.6513409961686, Blast_Score=87, Evalue=2e-18, Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=30.2325581395349, Blast_Score=86, Evalue=2e-18, Organism=Escherichia coli, GI1787526, Length=261, Percent_Identity=27.9693486590038, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI1787905, Length=246, Percent_Identity=28.8617886178862, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI2367365, Length=256, Percent_Identity=28.90625, Blast_Score=63, Evalue=2e-11, Organism=Escherichia coli, GI1789057, Length=257, Percent_Identity=26.0700389105058, Blast_Score=62, Evalue=3e-11, Organism=Caenorhabditis elegans, GI17562910, Length=268, Percent_Identity=32.089552238806, Blast_Score=109, Evalue=2e-24, Organism=Caenorhabditis elegans, GI71994600, Length=258, Percent_Identity=34.1085271317829, Blast_Score=102, Evalue=3e-22, Organism=Caenorhabditis elegans, GI72000259, Length=258, Percent_Identity=31.3953488372093, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI71994604, Length=259, Percent_Identity=36.6795366795367, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=32.3420074349442, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=34.0080971659919, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=31.5589353612167, Blast_Score=95, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17560220, Length=273, Percent_Identity=32.2344322344322, Blast_Score=95, Evalue=4e-20, Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=33.2089552238806, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI193204405, Length=271, Percent_Identity=31.3653136531365, Blast_Score=93, Evalue=1e-19, Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=30.1587301587302, Blast_Score=91, Evalue=6e-19, Organism=Caenorhabditis elegans, GI17544670, Length=271, Percent_Identity=29.8892988929889, Blast_Score=91, Evalue=8e-19, Organism=Caenorhabditis elegans, GI17538182, Length=208, Percent_Identity=33.6538461538462, Blast_Score=89, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17565030, Length=268, Percent_Identity=31.7164179104478, Blast_Score=88, Evalue=4e-18, Organism=Caenorhabditis elegans, GI17562990, Length=260, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17561402, Length=260, Percent_Identity=28.8461538461538, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17531453, Length=278, Percent_Identity=31.6546762589928, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=29.0836653386454, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17562908, Length=270, Percent_Identity=32.5925925925926, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17559104, Length=271, Percent_Identity=30.6273062730627, Blast_Score=84, Evalue=5e-17, Organism=Caenorhabditis elegans, GI115534694, Length=266, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17560150, Length=260, Percent_Identity=29.6153846153846, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17551412, Length=271, Percent_Identity=31.7343173431734, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=28.8389513108614, Blast_Score=80, Evalue=1e-15, Organism=Caenorhabditis elegans, GI17562906, Length=270, Percent_Identity=32.5925925925926, Blast_Score=80, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17568967, Length=199, Percent_Identity=34.1708542713568, Blast_Score=74, Evalue=6e-14, Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=28.7313432835821, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI32563809, Length=259, Percent_Identity=26.2548262548263, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17567345, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17507613, Length=259, Percent_Identity=26.2548262548263, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=30.1020408163265, Blast_Score=67, Evalue=1e-11, Organism=Saccharomyces cerevisiae, GI6322861, Length=199, Percent_Identity=32.6633165829146, Blast_Score=86, Evalue=9e-18, Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=2e-16, Organism=Saccharomyces cerevisiae, GI6323882, Length=250, Percent_Identity=28.8, Blast_Score=77, Evalue=2e-15, Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=33.59375, Blast_Score=111, Evalue=6e-25, Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=32.8125, Blast_Score=106, Evalue=2e-23, Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=33.4645669291339, Blast_Score=103, Evalue=9e-23, Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=32.4110671936759, Blast_Score=103, Evalue=1e-22, Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=30.1158301158301, Blast_Score=92, Evalue=3e-19, Organism=Drosophila melanogaster, GI17737361, Length=267, Percent_Identity=29.5880149812734, Blast_Score=92, Evalue=5e-19, Organism=Drosophila melanogaster, GI24643142, Length=252, Percent_Identity=29.7619047619048, Blast_Score=82, Evalue=3e-16, Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=28.1124497991968, Blast_Score=73, Evalue=2e-13, Organism=Drosophila melanogaster, GI24580925, Length=195, Percent_Identity=31.7948717948718, Blast_Score=72, Evalue=4e-13, Organism=Drosophila melanogaster, GI24644337, Length=177, Percent_Identity=31.638418079096, Blast_Score=65, Evalue=7e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.100 [C]
Molecular weight: Translated: 27329; Mature: 27198
Theoretical pI: Translated: 6.12; Mature: 6.12
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAAGGDFQDFVVVVTGASTGLGRAIAVEVARRGAKAVVVNYARSASEAEETAALVRAEGA CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCC EGVLVQGDVASDEDCRKIAAAAQPFGRIDALFNNAGTTRFAAHGDLDAVSAQDFLDLYAV CCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH NVVGAYQMVRACRSLLEAAPRHGAVVNTASIAGVTGIGSSIPYAASKGAMTTMTLSLARA HHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH LAPKIRVNAVCPGFIDTPWFGKGMPDAAVDRVRANAEASTPLRAASTPQDIAEATVFLAA HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEC PASRHVTGETLLVDAGMHLGYAPTVAR CCCCCCCCCEEEEECCCCCCCCCCCCC >Mature Secondary Structure AAGGDFQDFVVVVTGASTGLGRAIAVEVARRGAKAVVVNYARSASEAEETAALVRAEGA CCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCC EGVLVQGDVASDEDCRKIAAAAQPFGRIDALFNNAGTTRFAAHGDLDAVSAQDFLDLYAV CCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH NVVGAYQMVRACRSLLEAAPRHGAVVNTASIAGVTGIGSSIPYAASKGAMTTMTLSLARA HHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH LAPKIRVNAVCPGFIDTPWFGKGMPDAAVDRVRANAEASTPLRAASTPQDIAEATVFLAA HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEC PASRHVTGETLLVDAGMHLGYAPTVAR CCCCCCCCCEEEEECCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NADPH [C]
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]
Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]
General reaction: Redox reaction [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 2583128; 9831526 [H]