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The map label for this gene is yngI [H]

Identifier: 197105528

GI number: 197105528

Start: 2306817

End: 2308451

Strand: Direct

Name: yngI [H]

Synonym: PHZ_c2065

Alternate gene names: 197105528

Gene position: 2306817-2308451 (Clockwise)

Preceding gene: 197105525

Following gene: 197105533

Centisome position: 57.72

GC content: 72.48

Gene sequence:

>1635_bases
ATGGCGGGATGGACCCTGGTCGCGGTGTGGGAGGCGATCGCCGCGGCCCAGCCGGAGCGGCCGGCCCTGATCCAGGGCGA
GCGGACCGTGAGCTGGGGCGCCTTCGACGCCCGGGCCGACGCCCTGGCGGCGCACCTGATCGGCAAGGGGCTGGGCCGGC
AGGCCAAGGTGGCCGCCTACCTGTTCAACGGACCCGAGTACCTCGAGACCTATTTCGCCGCGTTCAAGGGCGGCTTCGCC
CCGGTGAACACCAACTACCGGTACGGGCCCGAGGAGCTGCTGTACCTGTTCGACAACGCCGACGCCGAGGCGATCGTGTT
CCACGCGGGCTTCGCCGGGACCCTGGAGGCGATCCGCGACCGCCTGCCGAGGGTGAAGGCCTGGGTCGCCGTGGCCGAGC
CGGGCCACGCCGTCCCGGCCTGGGCCGAGGACTACGAGGCCATCGCGGGGCGCACGCCGGCGGAACGGCCCGCGCAGGCG
CCCTGGGGACGCTCGGGCGACGACCTCCTGCTGCTCTACACCGGCGGCACCACCGGCATGCCCAAGGGCGTGATGTGGCG
CCAGGACGACCTGTTCAACGTGATCGGGGCCGGCGGCCACGCGGCCATGGGCGTGGAGCCTGTGACCTCGCTGGAGGAAG
TGGCGGCCCGCCTGGAGAGCCCCGGACACGTCCGCCCCACCGCCCTGATCGCCTGCCCGCTGATGCACGGCACCGGCCAG
TTCTCGGCCTTCATCACCCTGAACCTGGGCGGGACGGTCGCCACCCTGCCCTCGCGCAAGTTCGACGCCGCCGAGCTGTG
GAACGAGGCCGAGCGCCTGAAGGCCGACAGCATCGCCATCGTCGGCCTGGCGTTCTCCACCCCCATGCTGGAGGCCCTGG
AGGCCCACCCCGGCCGCTGGGATCTGTCGTCGGTGAAGCTGATGAGCTCGTCCGGCTCGATGTGGAGCCAGGAGAACAAG
CGCGGGCTGCTGCGCCACGCCACCAATGCGGTGATCTACGACAGCCTGGGCTCTTCCGAAGCCGTGGGCCTGGGCGCCTC
GGCCTCGGCGCCTGGCCAGGAGGCGGCCACGGCGGCCTTCATGCTGGGCCCCAACTGCGCGGTGTTCACCGAGGACGGGC
GGCGGGTCGAGCCGGGCTCGGGCGAGCGCGGCATGGTGGCGGTCTCGGGCTTCCTGCCGACGGGCTACTACAAGGACCCG
GAGAAGAGCGCCAAGACGTTCCGGACCTTCGAGGGCCAGCGCTGGTCGGTGCCCGGCGACTGGGCCGAGGTGAACCCCGA
CGGCACGCTGAAGCTGCTGGGCCGCGGCTCGGTCTGCATCAACACCGGCGGCGAAAAGGTCTTCCCCGAGGAGGTGGAGG
AGGCCCTGAAGACCCATCCGGCGGTGCGCGACGCCGTGGTGGTGGGCGTGCCCGATCCGCGCTTCGGCGAGCGGATCTGC
GCCGTCGTCGAGCCCGAGGCGAACACCGAGCCCACGCTCGCGGACCTGTCGCAGCACGTGCACGGCCGCCTGGCCGGCTA
CAAGGCCCCGCGCGAACTGGTGCTGGTCGAGAGCATCGGCCGGGCCCCCAACGGCAAGGTGGACTACAAGGCCGTGAAGG
AGCGCGCCCTGGCCGAGCTGGGGACGCCGGCCTAG

Upstream 100 bases:

>100_bases
GGACGCGCCCGCGGGGGGGACCCGGCGGCGTTTGACTTCCCGGGCGTCCGCCCCCTCAACTGGCCGGCAAAAGACCAGAA
GAGACGTTGGGAGGAATGCG

Downstream 100 bases:

>100_bases
CCGAGGGGCCTAGCCGAGGGGCCTAGCCGAGGGGCCTAGCGGCTGCCGCGCGGTTCGCGCGACAGGCGGGTCATCAGCAG
CGCGGCCCGCTTGGCCTGGA

Product: CoA synthetase, long-chain fatty acid:CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MAGWTLVAVWEAIAAAQPERPALIQGERTVSWGAFDARADALAAHLIGKGLGRQAKVAAYLFNGPEYLETYFAAFKGGFA
PVNTNYRYGPEELLYLFDNADAEAIVFHAGFAGTLEAIRDRLPRVKAWVAVAEPGHAVPAWAEDYEAIAGRTPAERPAQA
PWGRSGDDLLLLYTGGTTGMPKGVMWRQDDLFNVIGAGGHAAMGVEPVTSLEEVAARLESPGHVRPTALIACPLMHGTGQ
FSAFITLNLGGTVATLPSRKFDAAELWNEAERLKADSIAIVGLAFSTPMLEALEAHPGRWDLSSVKLMSSSGSMWSQENK
RGLLRHATNAVIYDSLGSSEAVGLGASASAPGQEAATAAFMLGPNCAVFTEDGRRVEPGSGERGMVAVSGFLPTGYYKDP
EKSAKTFRTFEGQRWSVPGDWAEVNPDGTLKLLGRGSVCINTGGEKVFPEEVEEALKTHPAVRDAVVVGVPDPRFGERIC
AVVEPEANTEPTLADLSQHVHGRLAGYKAPRELVLVESIGRAPNGKVDYKAVKERALAELGTPA

Sequences:

>Translated_544_residues
MAGWTLVAVWEAIAAAQPERPALIQGERTVSWGAFDARADALAAHLIGKGLGRQAKVAAYLFNGPEYLETYFAAFKGGFA
PVNTNYRYGPEELLYLFDNADAEAIVFHAGFAGTLEAIRDRLPRVKAWVAVAEPGHAVPAWAEDYEAIAGRTPAERPAQA
PWGRSGDDLLLLYTGGTTGMPKGVMWRQDDLFNVIGAGGHAAMGVEPVTSLEEVAARLESPGHVRPTALIACPLMHGTGQ
FSAFITLNLGGTVATLPSRKFDAAELWNEAERLKADSIAIVGLAFSTPMLEALEAHPGRWDLSSVKLMSSSGSMWSQENK
RGLLRHATNAVIYDSLGSSEAVGLGASASAPGQEAATAAFMLGPNCAVFTEDGRRVEPGSGERGMVAVSGFLPTGYYKDP
EKSAKTFRTFEGQRWSVPGDWAEVNPDGTLKLLGRGSVCINTGGEKVFPEEVEEALKTHPAVRDAVVVGVPDPRFGERIC
AVVEPEANTEPTLADLSQHVHGRLAGYKAPRELVLVESIGRAPNGKVDYKAVKERALAELGTPA
>Mature_543_residues
AGWTLVAVWEAIAAAQPERPALIQGERTVSWGAFDARADALAAHLIGKGLGRQAKVAAYLFNGPEYLETYFAAFKGGFAP
VNTNYRYGPEELLYLFDNADAEAIVFHAGFAGTLEAIRDRLPRVKAWVAVAEPGHAVPAWAEDYEAIAGRTPAERPAQAP
WGRSGDDLLLLYTGGTTGMPKGVMWRQDDLFNVIGAGGHAAMGVEPVTSLEEVAARLESPGHVRPTALIACPLMHGTGQF
SAFITLNLGGTVATLPSRKFDAAELWNEAERLKADSIAIVGLAFSTPMLEALEAHPGRWDLSSVKLMSSSGSMWSQENKR
GLLRHATNAVIYDSLGSSEAVGLGASASAPGQEAATAAFMLGPNCAVFTEDGRRVEPGSGERGMVAVSGFLPTGYYKDPE
KSAKTFRTFEGQRWSVPGDWAEVNPDGTLKLLGRGSVCINTGGEKVFPEEVEEALKTHPAVRDAVVVGVPDPRFGERICA
VVEPEANTEPTLADLSQHVHGRLAGYKAPRELVLVESIGRAPNGKVDYKAVKERALAELGTPA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=573, Percent_Identity=22.6876090750436, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI38505220, Length=512, Percent_Identity=24.4140625, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI187761345, Length=418, Percent_Identity=28.7081339712919, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI187761343, Length=418, Percent_Identity=28.7081339712919, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI58082049, Length=511, Percent_Identity=22.1135029354207, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI157311624, Length=507, Percent_Identity=22.2879684418146, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI157311622, Length=507, Percent_Identity=22.2879684418146, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI122937307, Length=517, Percent_Identity=23.5976789168279, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI28416953, Length=523, Percent_Identity=23.7093690248566, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI115511026, Length=158, Percent_Identity=34.1772151898734, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI42544132, Length=504, Percent_Identity=20.6349206349206, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=576, Percent_Identity=24.6527777777778, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693145, Length=552, Percent_Identity=24.6376811594203, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1786810, Length=533, Percent_Identity=25.515947467167, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI221142682, Length=544, Percent_Identity=22.0588235294118, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=551, Percent_Identity=25.5898366606171, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=551, Percent_Identity=25.5898366606171, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=551, Percent_Identity=25.5898366606171, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=549, Percent_Identity=24.5901639344262, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=546, Percent_Identity=26.3736263736264, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=548, Percent_Identity=25.9124087591241, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=554, Percent_Identity=25.4512635379061, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=462, Percent_Identity=23.3766233766234, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=364, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=146, Percent_Identity=31.5068493150685, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=515, Percent_Identity=22.3300970873786, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=406, Percent_Identity=24.384236453202, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581924, Length=539, Percent_Identity=23.9332096474954, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859661, Length=549, Percent_Identity=24.2258652094718, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355181, Length=383, Percent_Identity=25.065274151436, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355225, Length=404, Percent_Identity=24.009900990099, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 57945; Mature: 57814

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGWTLVAVWEAIAAAQPERPALIQGERTVSWGAFDARADALAAHLIGKGLGRQAKVAAY
CCCCHHHHHHHHHHHCCCCCCCEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEE
LFNGPEYLETYFAAFKGGFAPVNTNYRYGPEELLYLFDNADAEAIVFHAGFAGTLEAIRD
EECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEECCCHHHHHHHHH
RLPRVKAWVAVAEPGHAVPAWAEDYEAIAGRTPAERPAQAPWGRSGDDLLLLYTGGTTGM
HCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
PKGVMWRQDDLFNVIGAGGHAAMGVEPVTSLEEVAARLESPGHVRPTALIACPLMHGTGQ
CCCCEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
FSAFITLNLGGTVATLPSRKFDAAELWNEAERLKADSIAIVGLAFSTPMLEALEAHPGRW
EEEEEEEECCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCC
DLSSVKLMSSSGSMWSQENKRGLLRHATNAVIYDSLGSSEAVGLGASASAPGQEAATAAF
CHHHEEEECCCCCCCCCHHHCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHEEEE
MLGPNCAVFTEDGRRVEPGSGERGMVAVSGFLPTGYYKDPEKSAKTFRTFEGQRWSVPGD
EECCCEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEECCCCC
WAEVNPDGTLKLLGRGSVCINTGGEKVFPEEVEEALKTHPAVRDAVVVGVPDPRFGERIC
CEEECCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEE
AVVEPEANTEPTLADLSQHVHGRLAGYKAPRELVLVESIGRAPNGKVDYKAVKERALAEL
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHC
GTPA
CCCC
>Mature Secondary Structure 
AGWTLVAVWEAIAAAQPERPALIQGERTVSWGAFDARADALAAHLIGKGLGRQAKVAAY
CCCHHHHHHHHHHHCCCCCCCEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEE
LFNGPEYLETYFAAFKGGFAPVNTNYRYGPEELLYLFDNADAEAIVFHAGFAGTLEAIRD
EECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEECCCHHHHHHHHH
RLPRVKAWVAVAEPGHAVPAWAEDYEAIAGRTPAERPAQAPWGRSGDDLLLLYTGGTTGM
HCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
PKGVMWRQDDLFNVIGAGGHAAMGVEPVTSLEEVAARLESPGHVRPTALIACPLMHGTGQ
CCCCEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
FSAFITLNLGGTVATLPSRKFDAAELWNEAERLKADSIAIVGLAFSTPMLEALEAHPGRW
EEEEEEEECCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCC
DLSSVKLMSSSGSMWSQENKRGLLRHATNAVIYDSLGSSEAVGLGASASAPGQEAATAAF
CHHHEEEECCCCCCCCCHHHCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHEEEE
MLGPNCAVFTEDGRRVEPGSGERGMVAVSGFLPTGYYKDPEKSAKTFRTFEGQRWSVPGD
EECCCEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEECCCCC
WAEVNPDGTLKLLGRGSVCINTGGEKVFPEEVEEALKTHPAVRDAVVVGVPDPRFGERIC
CEEECCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEE
AVVEPEANTEPTLADLSQHVHGRLAGYKAPRELVLVESIGRAPNGKVDYKAVKERALAEL
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHC
GTPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]