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The map label for this gene is spsK [H]

Identifier: 197105533

GI number: 197105533

Start: 2314203

End: 2316572

Strand: Direct

Name: spsK [H]

Synonym: PHZ_c2070

Alternate gene names: 197105533

Gene position: 2314203-2316572 (Clockwise)

Preceding gene: 197105528

Following gene: 197105539

Centisome position: 57.91

GC content: 74.01

Gene sequence:

>2370_bases
ATGCGGCCGATAATTCCCGCCCCCGGGCGCCGGTTCCAGCCCCATTCGGCAGGATGGGGAACGATTGCTTCCGCGGCGGC
TTTGAACCGTCGGGTTGTGACGGGAAAGCCAGGGTCGATGCCGACACTCGAGCTGTGGGGCGGTCACGAATGCACCGTGA
ACCGTGTGGGGACCGCGTTCCACGACCAGACGGTCAGGTCCGGGCACCACGACCGCATCGAGGACCTCGACCGCTTCGCC
GAGCTGGGCCTGAAGGCGCTGCGCTATCCGGTGTTGTGGGAGCGGACGGCCCCCGATCGGCCCGGCGAGCACGACTGGCG
TTGGACCGACGCGCGGCTGGCGCGCATCCGCGAGCTCGGCATGCGGCCGATCCTCGGCCTGCTCCACCACGGCAGCGGGC
CACGCTACACAAGCCTGGTGGACGAGGGCTTCGCGCGCCTGTTCGCCGGCTACGCCCGCGCCGCCGCCGAGCGGTATCCC
TGGGCGCGGGACTGGACCCCGATCAACGAGCCGCTGACCACCGCGCGCTTCTCGGCGCTCTATGGCCACTGGTATCCCCA
CCTGGCGGACGAGCCCTTCTTCTGGGCGGCCCTGCTCAACCAGATCGACGGCACGCGCCTGGCCATGCGGGAGATCCGCA
AGGTCAATCCCGACGCCCGGCTGATCCAGACCGAGGACCTCGGCCGCACCTACGCGACCCGCCTCCTCGCCGGCCAGGCC
GAGTTCGAGAACTGCCGGCGGTGGATGACCTGGGACCTGCTGGCGGGCCGCGTGACCCGCGGGCACCCGATGTTCGAGCG
GCTGGCGGCCTGCGGCTTCGCCGACCGGCTGGCGGCCATCGCCGACGATCCCTGCCCGCCCGACATCCTGGGCGTGAACC
ACTACCTGACCAGCGAGCGGTTCCTGGACCACCGGCTAGACGCCTATCCCCCCGAGCGACACGGCGGCAACGACCAGACG
GCCTATGCCGACGTGGAGGCCGTGCGGGTCGTCCTGCCCGCTCCCGGGGGCCTGGAGGGCGTGCTGGAGGAAGCCTGGGC
GCGCTACGGCCTGCCGCTGGCCGTCACCGAGGCCCACAACGGCTGCACCCGCGAGGAGCAGGTCCGCTGGCTGCGCGAGG
CCTGGGAGACCGCCGAGCGCCTGCAGGGCCGCGGCGTGGACATCCGCGCGGTCACCGCCTGGGCCCTGCTGGGCACGTTC
GACTGGAACAGCCTGCTCACCCGCGCCCTCGGCCACTACGAGGTCGGCGCCTTCGACGTCCGCGCCGAGCCGCCGCGTCC
TACCGCCATCGCCGCCGAGCTGAAGCGGCTCGCCCAGGGCGGGCCGCCGCATCCCGGGACGGAGGGCCCCGGCTGGTGGC
GGCGCGACATCCGCCTGGAGTTCCGGCCGGTGTTCCGGCACGTGGAGACCCCCGAGCCGCGCTCGGCCTGGCAGGCGGGC
CGCGGGGCGCGCCAGCCGCTGCTGATCGTCGGGGCCACCGGCACCCTGGGCAAGGCGCTGGCCCGCGCCTGCGAATGGCG
CGGCATCGACTATCGCCTGACCAGCCGGGCCGAGCTTTCCCTGGACGACGCCGACTCCATCGCCGCCGCCCTCGACGGCT
GCGGACCGTGGGCGGTGATCAACGCCGCCGGCTGGGTGCGGGTGGACGAGGCCGAGGCCTGCGCCGCGGACTGCCTGGCG
GCCAACGCCGACGGCGCGGTGCGGCTCGCGCGGATCTGCGCCGAACGGGGCCTGCCCCTGGTGGGCTATTCCAGCGACCT
GGTGTTCGACGGCTCGGCCGGGCGGCCGTACGTGGAGAGCGACCCGCCGGCGCCGCTGAACGTCTACGGCGAGAGCAAGG
CCCGCGCCGAGCGCGAGGTGCTGGCCCTGGGCGGGCAGGCGCTGATGATCCGCACCGCGGCGTTCTTCTCGCCGTTCGAT
CCCTACAACTTCGCGGCCCATGTCGTGGGGACGCTGTCGGCGGGACGCGAGTTCGTGGCCGCCGAGGACCTGGTCGTCTC
GCCGACCTACGTGCCGGACCTGGTGGACGCCAGCCTCGACCTGCTGCTGGACGGCGACGCCGGCCTGCGCCACCTGGCCA
ACGCCGGAGAGGTGAGCTGGGCCGGCTTCGCCCGGATGATCGCCGCCGAGCTGGGCCTCGACGCCGGCCGGGTGCTCGGC
GTCCGGGCCGACAGCTTCGGCTGGGCCGCGGCGCGGCCGCAGCACGTCCCGCTCTCGACCGAGCGGGGCCGGGTCATGCC
GAGCCTCGAGAATGCGGTCGCCCGCTACGCCGCCATCGTGCGGGAAGCCGAGTTCGCGCCCGAGGCCGAGGCCATGGCCG
ACCTCGCCCATCCGGAGACGCTGGTGCGCCGGGGCGCGCCGCCGACCTGA

Upstream 100 bases:

>100_bases
CACGGGGAAGAATCCCCGCTCGCTCAGAGCCTTCGCGGTGTGTGCGCCGATGTAGCCGGCTCCGCCCGTCACCAGGACGG
CGGGCTTCGATCCCCGATCC

Downstream 100 bases:

>100_bases
CGGCGCGCGGCCGCCGCGTCAGTGGCTGGCCTGGTCGTCCTCGACCTCGAAGACGTCCTCCTCCACTTCCGAGAGCAGCT
CGTCCAGGGCGTCGATGATC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MRPIIPAPGRRFQPHSAGWGTIASAAALNRRVVTGKPGSMPTLELWGGHECTVNRVGTAFHDQTVRSGHHDRIEDLDRFA
ELGLKALRYPVLWERTAPDRPGEHDWRWTDARLARIRELGMRPILGLLHHGSGPRYTSLVDEGFARLFAGYARAAAERYP
WARDWTPINEPLTTARFSALYGHWYPHLADEPFFWAALLNQIDGTRLAMREIRKVNPDARLIQTEDLGRTYATRLLAGQA
EFENCRRWMTWDLLAGRVTRGHPMFERLAACGFADRLAAIADDPCPPDILGVNHYLTSERFLDHRLDAYPPERHGGNDQT
AYADVEAVRVVLPAPGGLEGVLEEAWARYGLPLAVTEAHNGCTREEQVRWLREAWETAERLQGRGVDIRAVTAWALLGTF
DWNSLLTRALGHYEVGAFDVRAEPPRPTAIAAELKRLAQGGPPHPGTEGPGWWRRDIRLEFRPVFRHVETPEPRSAWQAG
RGARQPLLIVGATGTLGKALARACEWRGIDYRLTSRAELSLDDADSIAAALDGCGPWAVINAAGWVRVDEAEACAADCLA
ANADGAVRLARICAERGLPLVGYSSDLVFDGSAGRPYVESDPPAPLNVYGESKARAEREVLALGGQALMIRTAAFFSPFD
PYNFAAHVVGTLSAGREFVAAEDLVVSPTYVPDLVDASLDLLLDGDAGLRHLANAGEVSWAGFARMIAAELGLDAGRVLG
VRADSFGWAAARPQHVPLSTERGRVMPSLENAVARYAAIVREAEFAPEAEAMADLAHPETLVRRGAPPT

Sequences:

>Translated_789_residues
MRPIIPAPGRRFQPHSAGWGTIASAAALNRRVVTGKPGSMPTLELWGGHECTVNRVGTAFHDQTVRSGHHDRIEDLDRFA
ELGLKALRYPVLWERTAPDRPGEHDWRWTDARLARIRELGMRPILGLLHHGSGPRYTSLVDEGFARLFAGYARAAAERYP
WARDWTPINEPLTTARFSALYGHWYPHLADEPFFWAALLNQIDGTRLAMREIRKVNPDARLIQTEDLGRTYATRLLAGQA
EFENCRRWMTWDLLAGRVTRGHPMFERLAACGFADRLAAIADDPCPPDILGVNHYLTSERFLDHRLDAYPPERHGGNDQT
AYADVEAVRVVLPAPGGLEGVLEEAWARYGLPLAVTEAHNGCTREEQVRWLREAWETAERLQGRGVDIRAVTAWALLGTF
DWNSLLTRALGHYEVGAFDVRAEPPRPTAIAAELKRLAQGGPPHPGTEGPGWWRRDIRLEFRPVFRHVETPEPRSAWQAG
RGARQPLLIVGATGTLGKALARACEWRGIDYRLTSRAELSLDDADSIAAALDGCGPWAVINAAGWVRVDEAEACAADCLA
ANADGAVRLARICAERGLPLVGYSSDLVFDGSAGRPYVESDPPAPLNVYGESKARAEREVLALGGQALMIRTAAFFSPFD
PYNFAAHVVGTLSAGREFVAAEDLVVSPTYVPDLVDASLDLLLDGDAGLRHLANAGEVSWAGFARMIAAELGLDAGRVLG
VRADSFGWAAARPQHVPLSTERGRVMPSLENAVARYAAIVREAEFAPEAEAMADLAHPETLVRRGAPPT
>Mature_789_residues
MRPIIPAPGRRFQPHSAGWGTIASAAALNRRVVTGKPGSMPTLELWGGHECTVNRVGTAFHDQTVRSGHHDRIEDLDRFA
ELGLKALRYPVLWERTAPDRPGEHDWRWTDARLARIRELGMRPILGLLHHGSGPRYTSLVDEGFARLFAGYARAAAERYP
WARDWTPINEPLTTARFSALYGHWYPHLADEPFFWAALLNQIDGTRLAMREIRKVNPDARLIQTEDLGRTYATRLLAGQA
EFENCRRWMTWDLLAGRVTRGHPMFERLAACGFADRLAAIADDPCPPDILGVNHYLTSERFLDHRLDAYPPERHGGNDQT
AYADVEAVRVVLPAPGGLEGVLEEAWARYGLPLAVTEAHNGCTREEQVRWLREAWETAERLQGRGVDIRAVTAWALLGTF
DWNSLLTRALGHYEVGAFDVRAEPPRPTAIAAELKRLAQGGPPHPGTEGPGWWRRDIRLEFRPVFRHVETPEPRSAWQAG
RGARQPLLIVGATGTLGKALARACEWRGIDYRLTSRAELSLDDADSIAAALDGCGPWAVINAAGWVRVDEAEACAADCLA
ANADGAVRLARICAERGLPLVGYSSDLVFDGSAGRPYVESDPPAPLNVYGESKARAEREVLALGGQALMIRTAAFFSPFD
PYNFAAHVVGTLSAGREFVAAEDLVVSPTYVPDLVDASLDLLLDGDAGLRHLANAGEVSWAGFARMIAAELGLDAGRVLG
VRADSFGWAAARPQHVPLSTERGRVMPSLENAVARYAAIVREAEFAPEAEAMADLAHPETLVRRGAPPT

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Escherichia coli, GI1788352, Length=287, Percent_Identity=27.1777003484321, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 86593; Mature: 86593

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIIPAPGRRFQPHSAGWGTIASAAALNRRVVTGKPGSMPTLELWGGHECTVNRVGTAF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCEEEECCCCEEEHHHCCCHH
HDQTVRSGHHDRIEDLDRFAELGLKALRYPVLWERTAPDRPGEHDWRWTDARLARIRELG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHC
MRPILGLLHHGSGPRYTSLVDEGFARLFAGYARAAAERYPWARDWTPINEPLTTARFSAL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
YGHWYPHLADEPFFWAALLNQIDGTRLAMREIRKVNPDARLIQTEDLGRTYATRLLAGQA
HHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCH
EFENCRRWMTWDLLAGRVTRGHPMFERLAACGFADRLAAIADDPCPPDILGVNHYLTSER
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
FLDHRLDAYPPERHGGNDQTAYADVEAVRVVLPAPGGLEGVLEEAWARYGLPLAVTEAHN
HHHHHHCCCCCCCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCC
GCTREEQVRWLREAWETAERLQGRGVDIRAVTAWALLGTFDWNSLLTRALGHYEVGAFDV
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEE
RAEPPRPTAIAAELKRLAQGGPPHPGTEGPGWWRRDIRLEFRPVFRHVETPEPRSAWQAG
ECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCEEEEEHHHHCCCCCCCHHHHHCC
RGARQPLLIVGATGTLGKALARACEWRGIDYRLTSRAELSLDDADSIAAALDGCGPWAVI
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCEECCCCHHHHHHHHCCCCCEEEE
NAAGWVRVDEAEACAADCLAANADGAVRLARICAERGLPLVGYSSDLVFDGSAGRPYVES
ECCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCC
DPPAPLNVYGESKARAEREVLALGGQALMIRTAAFFSPFDPYNFAAHVVGTLSAGREFVA
CCCCCEEECCCCHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCCEEE
AEDLVVSPTYVPDLVDASLDLLLDGDAGLRHLANAGEVSWAGFARMIAAELGLDAGRVLG
CCCEEECCCCCHHHHCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEE
VRADSFGWAAARPQHVPLSTERGRVMPSLENAVARYAAIVREAEFAPEAEAMADLAHPET
EECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
LVRRGAPPT
HHHCCCCCC
>Mature Secondary Structure
MRPIIPAPGRRFQPHSAGWGTIASAAALNRRVVTGKPGSMPTLELWGGHECTVNRVGTAF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCEEEECCCCEEEHHHCCCHH
HDQTVRSGHHDRIEDLDRFAELGLKALRYPVLWERTAPDRPGEHDWRWTDARLARIRELG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHC
MRPILGLLHHGSGPRYTSLVDEGFARLFAGYARAAAERYPWARDWTPINEPLTTARFSAL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
YGHWYPHLADEPFFWAALLNQIDGTRLAMREIRKVNPDARLIQTEDLGRTYATRLLAGQA
HHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCH
EFENCRRWMTWDLLAGRVTRGHPMFERLAACGFADRLAAIADDPCPPDILGVNHYLTSER
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
FLDHRLDAYPPERHGGNDQTAYADVEAVRVVLPAPGGLEGVLEEAWARYGLPLAVTEAHN
HHHHHHCCCCCCCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCC
GCTREEQVRWLREAWETAERLQGRGVDIRAVTAWALLGTFDWNSLLTRALGHYEVGAFDV
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEE
RAEPPRPTAIAAELKRLAQGGPPHPGTEGPGWWRRDIRLEFRPVFRHVETPEPRSAWQAG
ECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEECCCEEEEEHHHHCCCCCCCHHHHHCC
RGARQPLLIVGATGTLGKALARACEWRGIDYRLTSRAELSLDDADSIAAALDGCGPWAVI
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCEECCCCHHHHHHHHCCCCCEEEE
NAAGWVRVDEAEACAADCLAANADGAVRLARICAERGLPLVGYSSDLVFDGSAGRPYVES
ECCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCC
DPPAPLNVYGESKARAEREVLALGGQALMIRTAAFFSPFDPYNFAAHVVGTLSAGREFVA
CCCCCEEECCCCHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCCEEE
AEDLVVSPTYVPDLVDASLDLLLDGDAGLRHLANAGEVSWAGFARMIAAELGLDAGRVLG
CCCEEECCCCCHHHHCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEE
VRADSFGWAAARPQHVPLSTERGRVMPSLENAVARYAAIVREAEFAPEAEAMADLAHPET
EECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
LVRRGAPPT
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]