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The map label for this gene is nfdA [H]

Identifier: 197105464

GI number: 197105464

Start: 2235275

End: 2236870

Strand: Reverse

Name: nfdA [H]

Synonym: PHZ_c2001

Alternate gene names: 197105464

Gene position: 2236870-2235275 (Counterclockwise)

Preceding gene: 197105465

Following gene: 197105463

Centisome position: 55.97

GC content: 68.55

Gene sequence:

>1596_bases
GTGACGGTGGACGGGAAGGGCACGATCGCCGAGGCCGTCGCCGTGCGCGACGGCCGGATCGCGGCGATCGGCGACGCGGC
CGGCCTGGCCGCGTGGCGGGGGCCGGAGACGAAGGTCGTCGACGTGGGCGGCAAGACGGTCCTGCCGGGCTTCATCGACT
CCCACTCCCACATGGCGGGCATGGCGGAGGTCGAGGCGCTGCACATCAACGTGCATGCCCCGCCCTTGAAGAGCGGGAAG
GAGATCATCGCGACCCTCAAGGAGAAGACGAAGGGGCTTCCCAAGGGTGCGTGGATCATCGGGCAGGGCACCTACAACCA
GATCATGCCCACCCGCGAGGCGCTGGATAAGGCCTTCCCGGACAACCCGGTGCGGCTGATCTGGAGCGCCCACGACTCCA
TGATCAACCATGCCGCGGCCGTCGCGATGGGGCTCACCAAGGACTATCCCGATCCCAAGGACGGGATGGGCCGCTACGAG
CGCACCAAGACGGGCGAGGTGATGATCATCCGGGACGCCCCCGCGCCCTGGCCCAAGCTGCGCACCCTCACGCCCGAGGA
GCTGAAGGAGGGCGTGCGCACCATCATGACCGACTTCTATCTGAAGAAGGGGGTCACCACGGTTTCGGACTACGCCGCGC
CGCTGACGGTGAGGGCCTATCAGGAGCTGAAAGACGAGGGCCGCCTGCCGGTGCGGGTGAGCCTCAACTTCTTCGTCCGC
GACTACAAGCGCCTGGTCGGCAACCGCCTGCCGGGGCTGCTCGAGAGCGGCGCGCGCACGGGCCTCGGCGACGAGTGGCT
CCAGCTCGGCGCCCTGAAGTTCGCCGCCGACGGGGTGTGGGGCACGACCGCCTACACCTACCGGCCGCACTGGAACGGCT
CGGGCACGACCTGGATGCCGGACAACTACGGCGGCACCAATTTCACGCAGGAAGAGCTCAGCGATCAGATCCTGCAGGGG
CACAAGGCGGGCTGGCAGATCCACGTGCACGCCAACGGCGACCGTGCGCAGGACATGGCCCTCAACGCCTTCGAGGCCGC
GCAGGCCGCGGCGCCGAGGCCGGACGCGCGGCACCGGATCGAGCACTTCGGCCACTTCCTGACCCAGGACCCCGAGCGGA
CCGAGGCGCGCCTGGACCGGATGGTGAAGGGCGGGGTGATCCCGTCGCCGCAGGTCGCCTTCCTGTGGCGCCTGACCGAC
GTCAACGTGCGCGAGCCGGACGTGAAGTTCTTCGCCCTCAAGACCATGATCGACAAGGGCATGCACCCGGCCGGGGGGGT
CGACACCATCGGCACCCAGAACTTCGCGACGGCGCCGATGTTCTCGATCAGCCGGGCGATCCGCCGCGACACCAAGTACG
GCACGGTCGTCCAGCCCGAAGAGGCGATCTCGGTGATGGACGCCATACGCATGTTCACCATCTGGGGCGCCGAGGCCAAC
TTCATGGAGAAGGACCGCGGCAGCCTCGAGGTCGGCAAGCTCGCCGACATGGTCGTGCTGTCGCAGGATCCGCTGACGGC
GACGCCCGACCAGCTCCCCGGCATCGAGGCGGCGATGACGATCATCGACGGCAAGGTGGCGTACCAGAAGCCCTGA

Upstream 100 bases:

>100_bases
GCTCGTCACCGGCCTCGCCAGCGTCTGCGCCATCGGCGCTACGCCGGCGCTGGCGGCGCCTACCGCCTACGCCGACCTGC
TGCTGGTCAACGGGAAGGTC

Downstream 100 bases:

>100_bases
CAGACCTCCCCCTGTCGGGCTGAAGGCCCCGCATCGCCCTCCGCATGCTCGTGCGGAGGGCGATTTCGTTTGGGCGCCTA
TCGCAGCGATGTGGTGACTG

Product: amidohydrolase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTVDGKGTIAEAVAVRDGRIAAIGDAAGLAAWRGPETKVVDVGGKTVLPGFIDSHSHMAGMAEVEALHINVHAPPLKSGK
EIIATLKEKTKGLPKGAWIIGQGTYNQIMPTREALDKAFPDNPVRLIWSAHDSMINHAAAVAMGLTKDYPDPKDGMGRYE
RTKTGEVMIIRDAPAPWPKLRTLTPEELKEGVRTIMTDFYLKKGVTTVSDYAAPLTVRAYQELKDEGRLPVRVSLNFFVR
DYKRLVGNRLPGLLESGARTGLGDEWLQLGALKFAADGVWGTTAYTYRPHWNGSGTTWMPDNYGGTNFTQEELSDQILQG
HKAGWQIHVHANGDRAQDMALNAFEAAQAAAPRPDARHRIEHFGHFLTQDPERTEARLDRMVKGGVIPSPQVAFLWRLTD
VNVREPDVKFFALKTMIDKGMHPAGGVDTIGTQNFATAPMFSISRAIRRDTKYGTVVQPEEAISVMDAIRMFTIWGAEAN
FMEKDRGSLEVGKLADMVVLSQDPLTATPDQLPGIEAAMTIIDGKVAYQKP

Sequences:

>Translated_531_residues
MTVDGKGTIAEAVAVRDGRIAAIGDAAGLAAWRGPETKVVDVGGKTVLPGFIDSHSHMAGMAEVEALHINVHAPPLKSGK
EIIATLKEKTKGLPKGAWIIGQGTYNQIMPTREALDKAFPDNPVRLIWSAHDSMINHAAAVAMGLTKDYPDPKDGMGRYE
RTKTGEVMIIRDAPAPWPKLRTLTPEELKEGVRTIMTDFYLKKGVTTVSDYAAPLTVRAYQELKDEGRLPVRVSLNFFVR
DYKRLVGNRLPGLLESGARTGLGDEWLQLGALKFAADGVWGTTAYTYRPHWNGSGTTWMPDNYGGTNFTQEELSDQILQG
HKAGWQIHVHANGDRAQDMALNAFEAAQAAAPRPDARHRIEHFGHFLTQDPERTEARLDRMVKGGVIPSPQVAFLWRLTD
VNVREPDVKFFALKTMIDKGMHPAGGVDTIGTQNFATAPMFSISRAIRRDTKYGTVVQPEEAISVMDAIRMFTIWGAEAN
FMEKDRGSLEVGKLADMVVLSQDPLTATPDQLPGIEAAMTIIDGKVAYQKP
>Mature_530_residues
TVDGKGTIAEAVAVRDGRIAAIGDAAGLAAWRGPETKVVDVGGKTVLPGFIDSHSHMAGMAEVEALHINVHAPPLKSGKE
IIATLKEKTKGLPKGAWIIGQGTYNQIMPTREALDKAFPDNPVRLIWSAHDSMINHAAAVAMGLTKDYPDPKDGMGRYER
TKTGEVMIIRDAPAPWPKLRTLTPEELKEGVRTIMTDFYLKKGVTTVSDYAAPLTVRAYQELKDEGRLPVRVSLNFFVRD
YKRLVGNRLPGLLESGARTGLGDEWLQLGALKFAADGVWGTTAYTYRPHWNGSGTTWMPDNYGGTNFTQEELSDQILQGH
KAGWQIHVHANGDRAQDMALNAFEAAQAAAPRPDARHRIEHFGHFLTQDPERTEARLDRMVKGGVIPSPQVAFLWRLTDV
NVREPDVKFFALKTMIDKGMHPAGGVDTIGTQNFATAPMFSISRAIRRDTKYGTVVQPEEAISVMDAIRMFTIWGAEANF
MEKDRGSLEVGKLADMVVLSQDPLTATPDQLPGIEAAMTIIDGKVAYQKP

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 58218; Mature: 58087

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDGKGTIAEAVAVRDGRIAAIGDAAGLAAWRGPETKVVDVGGKTVLPGFIDSHSHMAG
CCCCCCCCHHHHEEECCCCEEEECCCCCHHHCCCCCCEEEECCCCEECCCHHCCCHHHCC
MAEVEALHINVHAPPLKSGKEIIATLKEKTKGLPKGAWIIGQGTYNQIMPTREALDKAFP
CEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCCCHHHHHHHCC
DNPVRLIWSAHDSMINHAAAVAMGLTKDYPDPKDGMGRYERTKTGEVMIIRDAPAPWPKL
CCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCC
RTLTPEELKEGVRTIMTDFYLKKGVTTVSDYAAPLTVRAYQELKDEGRLPVRVSLNFFVR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
DYKRLVGNRLPGLLESGARTGLGDEWLQLGALKFAADGVWGTTAYTYRPHWNGSGTTWMP
HHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCCCCCC
DNYGGTNFTQEELSDQILQGHKAGWQIHVHANGDRAQDMALNAFEAAQAAAPRPDARHRI
CCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHH
EHFGHFLTQDPERTEARLDRMVKGGVIPSPQVAFLWRLTDVNVREPDVKFFALKTMIDKG
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCC
MHPAGGVDTIGTQNFATAPMFSISRAIRRDTKYGTVVQPEEAISVMDAIRMFTIWGAEAN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHEECCCCC
FMEKDRGSLEVGKLADMVVLSQDPLTATPDQLPGIEAAMTIIDGKVAYQKP
CHHCCCCCEEHHHHHEEEEECCCCCCCCCCCCCCHHHHHHEECCEEECCCC
>Mature Secondary Structure 
TVDGKGTIAEAVAVRDGRIAAIGDAAGLAAWRGPETKVVDVGGKTVLPGFIDSHSHMAG
CCCCCCCHHHHEEECCCCEEEECCCCCHHHCCCCCCEEEECCCCEECCCHHCCCHHHCC
MAEVEALHINVHAPPLKSGKEIIATLKEKTKGLPKGAWIIGQGTYNQIMPTREALDKAFP
CEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHCCCCHHHHHHHCC
DNPVRLIWSAHDSMINHAAAVAMGLTKDYPDPKDGMGRYERTKTGEVMIIRDAPAPWPKL
CCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCC
RTLTPEELKEGVRTIMTDFYLKKGVTTVSDYAAPLTVRAYQELKDEGRLPVRVSLNFFVR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
DYKRLVGNRLPGLLESGARTGLGDEWLQLGALKFAADGVWGTTAYTYRPHWNGSGTTWMP
HHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCCCCCC
DNYGGTNFTQEELSDQILQGHKAGWQIHVHANGDRAQDMALNAFEAAQAAAPRPDARHRI
CCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHH
EHFGHFLTQDPERTEARLDRMVKGGVIPSPQVAFLWRLTDVNVREPDVKFFALKTMIDKG
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCC
MHPAGGVDTIGTQNFATAPMFSISRAIRRDTKYGTVVQPEEAISVMDAIRMFTIWGAEAN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHEECCCCC
FMEKDRGSLEVGKLADMVVLSQDPLTATPDQLPGIEAAMTIIDGKVAYQKP
CHHCCCCCEEHHHHHEEEEECCCCCCCCCCCCCCHHHHHHEECCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA