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The map label for this gene is puuE [H]

Identifier: 197105463

GI number: 197105463

Start: 2233795

End: 2235081

Strand: Reverse

Name: puuE [H]

Synonym: PHZ_c2000

Alternate gene names: 197105463

Gene position: 2235081-2233795 (Counterclockwise)

Preceding gene: 197105464

Following gene: 197105462

Centisome position: 55.93

GC content: 72.26

Gene sequence:

>1287_bases
GTGTCCCGGAACGCCGAACTTCTCAGCCGCCGCACCGCCGCCGTCTCGCGCGGCGTCAGCACCGCCACACCCGTGTTCGC
CGCGCGGGCCGAGAACGCGGAAATCTGGGACGCCGACGGCCGGCGCTATGTGGACTTCGCAGGCGGCATCGCGGTGCTCA
ACGTCGGCCACCGGCATCCGAAGGTGATCGAGGCCGTCCGGGCCCAGCTGGACGCCTATACCCACACCGCCTTCCAGGTC
ATGGCCTACGAGCCCTACATCGAACTGGCCGAGCGCCTGAACGCGCTGGCGCCGTTCAAGGGACCGGCCAAGACGCTGTT
CTTCACCACCGGCGCCGAAGCGGTGGAGAACGCCGTCAAGATCGCCCGGGTCGCCACCGGCCGCACGGCCGTGATCGCCT
TCACCGGCGCGTTCCACGGCCGGACCATGCTCACCATGGGCCTGACCGGCAAGGTCGCGCCCTACAAGAAGAACTTCGGC
CCGTCGCCGGCCGAGCTCTACCATCTGCCGTTCCCGGGCAGCCGCGCCGGCGTCAGCGTCGACGACACCCTGCGGGCGCT
GGATCTGCTGTTCGCCGCCGACGTGGCCCCTGACCGCGTGGCCGCGATCATCATCGAGCCGGTGCAGGGGGAGGGCGGCT
TCAACCCCGCGCCGGTCGAGCTGATGACGGCGCTGCGCCGCGTCTGCGACGAGCACGGAATCCTGCTCATCGCCGACGAG
GTCCAGACCGGCTTCGCCCGCACCGGCAAGATGTTCGGCATCGAGCACTATCCGGTGGAGCCGGATCTCGTGCCGGTCGC
CAAGTCGCTGGCCGGCGGCTTCCCGCTGTCGGGGGTCATCGGCCGCGCCGACCTGATGGACGTGGTGGACCCGGGCGGAC
TGGGCGGCACCTACGGAGGCTCGCCGGTGGCCTGCGCGGCGGCGCTCGCCGTGCTCGACGTGATCGCCGAGGAGGGGCTG
CTCGAGCGCGCCGGCGCGATCGGCTCGGCCATGCGCCGACGCTTGTCGGCCATCGCCGCCCGCAACGACGTCGCGCCCAT
CTCGGAGCCGCGCGGCCCGGGCGCGATGGTCGCGTTCGACATGCTCAAGAGCCGTGGCGGCGACGAGCCCGACGGCGCGG
CGGCGAAGGCCGTCGTCTCCCGCGCGCTGGACGCGGGGCTGGTGCTGCTGTCCTGCGGCCGCTACGGCGAGACCATCCGG
CTGCTCGTGCCGCTGACGGTCTCGGACGAGGTGCTGGCCGAGGGGATGGACATCCTGGAAGGCGCTCTCCGGGACGTGGC
GCCGTGA

Upstream 100 bases:

>100_bases
GTGACTGGAAGTCACCGTTTTCGGCGGCGTTGTCGCCTGTTGGCCGGCCGGGGATTTGGCGAGGCTGCGGGTCGGAACCT
GTACCTTCGAGAGTCTCCCC

Downstream 100 bases:

>100_bases
CCGCCGCCGCTCCCCGGACCGACGAGGCGGCCCGGCTGTCGCTCCGCAGGCCGGACCTGCTGCGCGAAGCCGCGTTGATC
GATGGGCGCTGGGTCTCCGG

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSRNAELLSRRTAAVSRGVSTATPVFAARAENAEIWDADGRRYVDFAGGIAVLNVGHRHPKVIEAVRAQLDAYTHTAFQV
MAYEPYIELAERLNALAPFKGPAKTLFFTTGAEAVENAVKIARVATGRTAVIAFTGAFHGRTMLTMGLTGKVAPYKKNFG
PSPAELYHLPFPGSRAGVSVDDTLRALDLLFAADVAPDRVAAIIIEPVQGEGGFNPAPVELMTALRRVCDEHGILLIADE
VQTGFARTGKMFGIEHYPVEPDLVPVAKSLAGGFPLSGVIGRADLMDVVDPGGLGGTYGGSPVACAAALAVLDVIAEEGL
LERAGAIGSAMRRRLSAIAARNDVAPISEPRGPGAMVAFDMLKSRGGDEPDGAAAKAVVSRALDAGLVLLSCGRYGETIR
LLVPLTVSDEVLAEGMDILEGALRDVAP

Sequences:

>Translated_428_residues
MSRNAELLSRRTAAVSRGVSTATPVFAARAENAEIWDADGRRYVDFAGGIAVLNVGHRHPKVIEAVRAQLDAYTHTAFQV
MAYEPYIELAERLNALAPFKGPAKTLFFTTGAEAVENAVKIARVATGRTAVIAFTGAFHGRTMLTMGLTGKVAPYKKNFG
PSPAELYHLPFPGSRAGVSVDDTLRALDLLFAADVAPDRVAAIIIEPVQGEGGFNPAPVELMTALRRVCDEHGILLIADE
VQTGFARTGKMFGIEHYPVEPDLVPVAKSLAGGFPLSGVIGRADLMDVVDPGGLGGTYGGSPVACAAALAVLDVIAEEGL
LERAGAIGSAMRRRLSAIAARNDVAPISEPRGPGAMVAFDMLKSRGGDEPDGAAAKAVVSRALDAGLVLLSCGRYGETIR
LLVPLTVSDEVLAEGMDILEGALRDVAP
>Mature_427_residues
SRNAELLSRRTAAVSRGVSTATPVFAARAENAEIWDADGRRYVDFAGGIAVLNVGHRHPKVIEAVRAQLDAYTHTAFQVM
AYEPYIELAERLNALAPFKGPAKTLFFTTGAEAVENAVKIARVATGRTAVIAFTGAFHGRTMLTMGLTGKVAPYKKNFGP
SPAELYHLPFPGSRAGVSVDDTLRALDLLFAADVAPDRVAAIIIEPVQGEGGFNPAPVELMTALRRVCDEHGILLIADEV
QTGFARTGKMFGIEHYPVEPDLVPVAKSLAGGFPLSGVIGRADLMDVVDPGGLGGTYGGSPVACAAALAVLDVIAEEGLL
ERAGAIGSAMRRRLSAIAARNDVAPISEPRGPGAMVAFDMLKSRGGDEPDGAAAKAVVSRALDAGLVLLSCGRYGETIRL
LVPLTVSDEVLAEGMDILEGALRDVAP

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=412, Percent_Identity=32.5242718446602, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI13994255, Length=373, Percent_Identity=32.7077747989276, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI37574042, Length=405, Percent_Identity=27.6543209876543, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI284507298, Length=326, Percent_Identity=32.5153374233129, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI226442705, Length=405, Percent_Identity=26.9135802469136, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI24119277, Length=412, Percent_Identity=26.4563106796117, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI226442709, Length=372, Percent_Identity=27.6881720430108, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI188536080, Length=429, Percent_Identity=26.5734265734266, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI38679950, Length=429, Percent_Identity=26.5734265734266, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI38679946, Length=429, Percent_Identity=26.5734265734266, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1787560, Length=422, Percent_Identity=56.3981042654028, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1789016, Length=419, Percent_Identity=52.5059665871122, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=35.6435643564356, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788044, Length=400, Percent_Identity=35.25, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI145693181, Length=386, Percent_Identity=35.2331606217617, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1786349, Length=340, Percent_Identity=31.4705882352941, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786991, Length=336, Percent_Identity=33.6309523809524, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32564660, Length=427, Percent_Identity=30.9133489461358, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI25144271, Length=415, Percent_Identity=30.1204819277108, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71992977, Length=446, Percent_Identity=28.9237668161435, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI25144274, Length=326, Percent_Identity=28.2208588957055, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=433, Percent_Identity=25.4041570438799, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=413, Percent_Identity=31.2348668280872, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6323470, Length=439, Percent_Identity=32.8018223234624, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324386, Length=324, Percent_Identity=24.0740740740741, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321456, Length=378, Percent_Identity=26.984126984127, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21357415, Length=407, Percent_Identity=31.4496314496315, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI161085790, Length=417, Percent_Identity=29.9760191846523, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28574759, Length=421, Percent_Identity=29.6912114014252, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21356575, Length=408, Percent_Identity=30.3921568627451, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281366494, Length=396, Percent_Identity=26.5151515151515, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24667139, Length=396, Percent_Identity=26.5151515151515, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24667143, Length=396, Percent_Identity=26.5151515151515, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 44821; Mature: 44690

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNAELLSRRTAAVSRGVSTATPVFAARAENAEIWDADGRRYVDFAGGIAVLNVGHRHP
CCCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCEEECCCCCEEEEECCCEEEEECCCCCH
KVIEAVRAQLDAYTHTAFQVMAYEPYIELAERLNALAPFKGPAKTLFFTTGAEAVENAVK
HHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHH
IARVATGRTAVIAFTGAFHGRTMLTMGLTGKVAPYKKNFGPSPAELYHLPFPGSRAGVSV
HHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHEEECCCCCCCCCCCH
DDTLRALDLLFAADVAPDRVAAIIIEPVQGEGGFNPAPVELMTALRRVCDEHGILLIADE
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECC
VQTGFARTGKMFGIEHYPVEPDLVPVAKSLAGGFPLSGVIGRADLMDVVDPGGLGGTYGG
HHHHHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCCCCCCCC
SPVACAAALAVLDVIAEEGLLERAGAIGSAMRRRLSAIAARNDVAPISEPRGPGAMVAFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
MLKSRGGDEPDGAAAKAVVSRALDAGLVLLSCGRYGETIRLLVPLTVSDEVLAEGMDILE
HHHHCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHH
GALRDVAP
HHHHHCCC
>Mature Secondary Structure 
SRNAELLSRRTAAVSRGVSTATPVFAARAENAEIWDADGRRYVDFAGGIAVLNVGHRHP
CCCHHHHHHHHHHHHCCCCCCCCHHEECCCCCEEECCCCCEEEEECCCEEEEECCCCCH
KVIEAVRAQLDAYTHTAFQVMAYEPYIELAERLNALAPFKGPAKTLFFTTGAEAVENAVK
HHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHH
IARVATGRTAVIAFTGAFHGRTMLTMGLTGKVAPYKKNFGPSPAELYHLPFPGSRAGVSV
HHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHEEECCCCCCCCCCCH
DDTLRALDLLFAADVAPDRVAAIIIEPVQGEGGFNPAPVELMTALRRVCDEHGILLIADE
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECC
VQTGFARTGKMFGIEHYPVEPDLVPVAKSLAGGFPLSGVIGRADLMDVVDPGGLGGTYGG
HHHHHHHCCCEECCCCCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCCCCCCCC
SPVACAAALAVLDVIAEEGLLERAGAIGSAMRRRLSAIAARNDVAPISEPRGPGAMVAFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
MLKSRGGDEPDGAAAKAVVSRALDAGLVLLSCGRYGETIRLLVPLTVSDEVLAEGMDILE
HHHHCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHH
GALRDVAP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]