Click here to switch to the map view.

The map label for this gene is gph [H]

Identifier: 197105454

GI number: 197105454

Start: 2224883

End: 2225578

Strand: Direct

Name: gph [H]

Synonym: PHZ_c1991

Alternate gene names: 197105454

Gene position: 2224883-2225578 (Clockwise)

Preceding gene: 197105447

Following gene: 197105471

Centisome position: 55.67

GC content: 72.7

Gene sequence:

>696_bases
ATGGCAGACAGCATCCTCGAAGGCGCCGTCGTCGCCTTCGATCTCGACGGCACCCTCGTCGACACCGCGCCCGACCTGAT
CGGGACGCTGAACGTGCTGCTGGCCGAGGAAGGCGTGCGGCCCTTCCCGCTGGAGCAGGCGCGGCCGTTCATCGGCAAGG
GGGCCCGCTGGCTGATCGAGCGCGGCTTCGCCGAGGCGGGCGCTCCGCTGGAGGCCGAGCGGGTCCCGGCGCTGTTCGAC
CGGTTCATCGCCCACTACCTGGAGCACATCGCCGACGAGAGCCGCCCCTTCCCCGGCTGCGTGGAGGCGCTGGACGTGCT
GCGCGGTGCTGGGGCGCGGCTCGCGGTGTGCACCAACAAGCTGACCGGGCTTTCGGCCTCGCTCCTGGACGCCCTGGGCC
TGGCCGACCGGTTCGAGGTCATCGTCGGGGCTGACGCCGCCCCGGCGATCAAGCCCGATCCGCGGCATCTCGCCGCCGCG
GTCGAGGCCGTGGGCGGGCAGATGGGCCGCACCATCATGGTGGGCGACGCCGCCACCGACGCCGGGGCGGCGCGCGCCGC
AGGGGCGGGCCTGATCCTCGTCAGCTTCGGCTACACCGAGACGCCCGTGGCCGAGCTCGATCCAGACGTCCTGATCCACC
ATTTCGACGAACTGCCGGGCGCCTGCGAAAGGCTCCTGGGCGCTTGCGGCGCGTAA

Upstream 100 bases:

>100_bases
TCAACGATGCCGAACGCCTTAGCAAGCCTTGTGGCGCCGCGCGACAAGAGGCTAGCTTTCGGCACGTTTCCGGAACCCGA
GCGCAGCAGCACCCCCGCCC

Downstream 100 bases:

>100_bases
GCCTTCCGGCTATACGCCCCCTCCTCCCGCGCGCCCAACCGAAGGGCGCGCTCCGGCGGATGCGTAGCTCAGCGGGAGAG
CACCTCGTTCACACCGAGGG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MADSILEGAVVAFDLDGTLVDTAPDLIGTLNVLLAEEGVRPFPLEQARPFIGKGARWLIERGFAEAGAPLEAERVPALFD
RFIAHYLEHIADESRPFPGCVEALDVLRGAGARLAVCTNKLTGLSASLLDALGLADRFEVIVGADAAPAIKPDPRHLAAA
VEAVGGQMGRTIMVGDAATDAGAARAAGAGLILVSFGYTETPVAELDPDVLIHHFDELPGACERLLGACGA

Sequences:

>Translated_231_residues
MADSILEGAVVAFDLDGTLVDTAPDLIGTLNVLLAEEGVRPFPLEQARPFIGKGARWLIERGFAEAGAPLEAERVPALFD
RFIAHYLEHIADESRPFPGCVEALDVLRGAGARLAVCTNKLTGLSASLLDALGLADRFEVIVGADAAPAIKPDPRHLAAA
VEAVGGQMGRTIMVGDAATDAGAARAAGAGLILVSFGYTETPVAELDPDVLIHHFDELPGACERLLGACGA
>Mature_230_residues
ADSILEGAVVAFDLDGTLVDTAPDLIGTLNVLLAEEGVRPFPLEQARPFIGKGARWLIERGFAEAGAPLEAERVPALFDR
FIAHYLEHIADESRPFPGCVEALDVLRGAGARLAVCTNKLTGLSASLLDALGLADRFEVIVGADAAPAIKPDPRHLAAAV
EAVGGQMGRTIMVGDAATDAGAARAAGAGLILVSFGYTETPVAELDPDVLIHHFDELPGACERLLGACGA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=229, Percent_Identity=33.1877729257642, Blast_Score=94, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23959; Mature: 23827

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSILEGAVVAFDLDGTLVDTAPDLIGTLNVLLAEEGVRPFPLEQARPFIGKGARWLIE
CCCCHHCCEEEEEECCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHH
RGFAEAGAPLEAERVPALFDRFIAHYLEHIADESRPFPGCVEALDVLRGAGARLAVCTNK
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCH
LTGLSASLLDALGLADRFEVIVGADAAPAIKPDPRHLAAAVEAVGGQMGRTIMVGDAATD
HCCCHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCC
AGAARAAGAGLILVSFGYTETPVAELDPDVLIHHFDELPGACERLLGACGA
CCHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ADSILEGAVVAFDLDGTLVDTAPDLIGTLNVLLAEEGVRPFPLEQARPFIGKGARWLIE
CCCHHCCEEEEEECCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHH
RGFAEAGAPLEAERVPALFDRFIAHYLEHIADESRPFPGCVEALDVLRGAGARLAVCTNK
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCH
LTGLSASLLDALGLADRFEVIVGADAAPAIKPDPRHLAAAVEAVGGQMGRTIMVGDAATD
HCCCHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCC
AGAARAAGAGLILVSFGYTETPVAELDPDVLIHHFDELPGACERLLGACGA
CCHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]