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The map label for this gene is btuB [H]

Identifier: 197105381

GI number: 197105381

Start: 2147782

End: 2149704

Strand: Reverse

Name: btuB [H]

Synonym: PHZ_c1918

Alternate gene names: 197105381

Gene position: 2149704-2147782 (Counterclockwise)

Preceding gene: 197105382

Following gene: 197105380

Centisome position: 53.79

GC content: 74.0

Gene sequence:

>1923_bases
GTGACGTGTCACGTCCCGCGAGGTTCGCGCATGAACTCCCGTATTCTTTCCCCCGCCGTCGCCGCGGGGACCCTGCTCAT
CTCCGGCCCGGCCTGGGCCGACGCCGCCACCGTCGACGACGTGGTGGTGACCGCCAATCGCCGCCCGACCCCCGCCGACG
CCGTCGGCAGCTCGGTCACCGTCATCCGCCGCGACGACATCGAGCGCTTCCAGTGGCGCAGCCTGCCCGAGGCGCTGCAG
CTCTCGCCCGGCCTCAACGTCGTCCAGACCGGCGGGACCGGCGGCCGCGCGTCGGTGTTCATCCGCGGCGCCAACGCCAA
CCACACCAAGGTGCTGATCGACGGCGTCGACGCCAACGATCCGAGCCAGGGCGGGGTCTTCGACTTCGGCGCGATCCTCA
CCTCCGACATCGAGCGGATCGAAGTCCTGCGGGGTCCCCAGAGCGGCCTCTACGGCTCGGACGCCATCGGCGGGGTGGTC
AACATCGTCACCCGCCCGCCCGGCGGGCCCCTGCGCCTGCGGGCCCGGCTTGAGGGCGGCGCGCACGAGACCTTCGACCA
GGGGGCGGGCGTCTCCGGACAGGCCGGGGTGGTCGGCTTCGCCTTCGACCTGGCCCACGCCCGCACGGGCGCGTCGGACA
CCACGCCCCTGGCCCTGCTGCCCGCCGGCCGCGCGCGCCACGCCGATTCCTACGAGAACCTGACGCTGTCGGGCCGGCTG
AACGCCCGTCCCTCCGCGCGCCTGGAGCTGGACGGGACCGTCCGATGGTCGGACGGCGACCTGCGCTTCACCGGCGATGA
GGGCTTCCCCAGCGCCCCGGCCGCCGAGCCGTCCCGCCAGGACGCGCGCAGCCTGGTCACCCGCGGCGAGGCGCGCATCG
ACGCCGTCCCGGATCGGCTGGAGGTCCGCGCCGGCGTGGGGCGGGCCGACTACCGCACCCGCCTGCAGGGCCCCGGCGGG
GCGGCTCCGACCTACAACAGGGGTGATCGCACCGAGGCCGACCTGCGCTTCGACCTGGTCCTGCCGCGGGGCGCCGCCCT
GGTGGCCGGGATCGAGGCCGAACGCGAGCGGCTGAAGGACCAGCCCGTCCGCTACCGGAACGACAATCGCGCGGCCTTCG
CCGAGCTGCACCTCGAGCCGGCCGGGGACCTCAACCTCACGGCCAGCGTCCGCTACGACGACAACGACCGGTTCGGCGGC
GAGGCCACCTGGCGGCTGGCGGGCGTCTACGAGCTGGCGGCCACGGGCGCCCGGCTGAAGGCCAGCTACGGGACGGGCTT
CAAGGCGCCCAGCCTGACCCAGCTCTTCGTCAGCTTCCCGGACTTCGGCTTCTTCGCCAATCCGGACCTCGACCCCGAGA
CGAGCCGCGGCTTCGACGTCGGCTTCGAGCAGCCCCTCGGCGGTCGGGTGGAGGTCGGCGCGACCTACTTCCGCCAGACC
ATCCGCAACCTGATCCAGTCCAACGCGGCCTTCACCTCCTACGAGAACGTCGGCCGGGCCCGCGCCGAGGGCGTGGAGGC
CTATGCGGCCCTCCGGCTCTCCGCCGCTGTGACCGCCCGGGCCGACTACACCTATGTCGACGCCGAGGACCGGGCGACGG
GGCTCGCGCTCCTGCGCCGGCCGAAGCACCGGGCCACGCTCAGCGCCGCCTGGACCCCCGCCGAGCGCGTCGCGCTCTCG
GGCTCGGTCGTCCACGTGGGCGGCTGGGTCGACGGCGACCGCAGCTTCACCGTCCCGCGCCTGAAGGCCGACGGCTACAC
GGTCGCGAACCTGGCCGGCGAGTACCGGCTGAGCGAGGCCGTCGTTCTGTTCGGCCGCGTCGAGAACCTGTTCGACGAGC
GGTTCGAGAATCCCGTGGGCTTCCAGCAGCCGGGGCGCGGCGCCTTCGCCGGCGTCCGGGTGCAGTGGGGGAGGGGCCAA
TGA

Upstream 100 bases:

>100_bases
AGCCGTGGTCACGCGCGAACGCGTCGGACGGGGTGCGCCGATGTGGATTGACCGGCCGTAGGGCGCTCCCGCGCCTCCGG
CCGGGCGGTCTCCATTCGCG

Downstream 100 bases:

>100_bases
GGGCCGAGTCTCTCCCGCTGCGGGAGGCCGCGTGGTCCGCGGGCCTCCTCGGCGCGGTGATCGTCGGGAGTCTCGCGCTC
TCCTGCCTGGAGCCGCTCGT

Product: TonB-dependent receptor for transport vitamin B12

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MTCHVPRGSRMNSRILSPAVAAGTLLISGPAWADAATVDDVVVTANRRPTPADAVGSSVTVIRRDDIERFQWRSLPEALQ
LSPGLNVVQTGGTGGRASVFIRGANANHTKVLIDGVDANDPSQGGVFDFGAILTSDIERIEVLRGPQSGLYGSDAIGGVV
NIVTRPPGGPLRLRARLEGGAHETFDQGAGVSGQAGVVGFAFDLAHARTGASDTTPLALLPAGRARHADSYENLTLSGRL
NARPSARLELDGTVRWSDGDLRFTGDEGFPSAPAAEPSRQDARSLVTRGEARIDAVPDRLEVRAGVGRADYRTRLQGPGG
AAPTYNRGDRTEADLRFDLVLPRGAALVAGIEAERERLKDQPVRYRNDNRAAFAELHLEPAGDLNLTASVRYDDNDRFGG
EATWRLAGVYELAATGARLKASYGTGFKAPSLTQLFVSFPDFGFFANPDLDPETSRGFDVGFEQPLGGRVEVGATYFRQT
IRNLIQSNAAFTSYENVGRARAEGVEAYAALRLSAAVTARADYTYVDAEDRATGLALLRRPKHRATLSAAWTPAERVALS
GSVVHVGGWVDGDRSFTVPRLKADGYTVANLAGEYRLSEAVVLFGRVENLFDERFENPVGFQQPGRGAFAGVRVQWGRGQ

Sequences:

>Translated_640_residues
MTCHVPRGSRMNSRILSPAVAAGTLLISGPAWADAATVDDVVVTANRRPTPADAVGSSVTVIRRDDIERFQWRSLPEALQ
LSPGLNVVQTGGTGGRASVFIRGANANHTKVLIDGVDANDPSQGGVFDFGAILTSDIERIEVLRGPQSGLYGSDAIGGVV
NIVTRPPGGPLRLRARLEGGAHETFDQGAGVSGQAGVVGFAFDLAHARTGASDTTPLALLPAGRARHADSYENLTLSGRL
NARPSARLELDGTVRWSDGDLRFTGDEGFPSAPAAEPSRQDARSLVTRGEARIDAVPDRLEVRAGVGRADYRTRLQGPGG
AAPTYNRGDRTEADLRFDLVLPRGAALVAGIEAERERLKDQPVRYRNDNRAAFAELHLEPAGDLNLTASVRYDDNDRFGG
EATWRLAGVYELAATGARLKASYGTGFKAPSLTQLFVSFPDFGFFANPDLDPETSRGFDVGFEQPLGGRVEVGATYFRQT
IRNLIQSNAAFTSYENVGRARAEGVEAYAALRLSAAVTARADYTYVDAEDRATGLALLRRPKHRATLSAAWTPAERVALS
GSVVHVGGWVDGDRSFTVPRLKADGYTVANLAGEYRLSEAVVLFGRVENLFDERFENPVGFQQPGRGAFAGVRVQWGRGQ
>Mature_639_residues
TCHVPRGSRMNSRILSPAVAAGTLLISGPAWADAATVDDVVVTANRRPTPADAVGSSVTVIRRDDIERFQWRSLPEALQL
SPGLNVVQTGGTGGRASVFIRGANANHTKVLIDGVDANDPSQGGVFDFGAILTSDIERIEVLRGPQSGLYGSDAIGGVVN
IVTRPPGGPLRLRARLEGGAHETFDQGAGVSGQAGVVGFAFDLAHARTGASDTTPLALLPAGRARHADSYENLTLSGRLN
ARPSARLELDGTVRWSDGDLRFTGDEGFPSAPAAEPSRQDARSLVTRGEARIDAVPDRLEVRAGVGRADYRTRLQGPGGA
APTYNRGDRTEADLRFDLVLPRGAALVAGIEAERERLKDQPVRYRNDNRAAFAELHLEPAGDLNLTASVRYDDNDRFGGE
ATWRLAGVYELAATGARLKASYGTGFKAPSLTQLFVSFPDFGFFANPDLDPETSRGFDVGFEQPLGGRVEVGATYFRQTI
RNLIQSNAAFTSYENVGRARAEGVEAYAALRLSAAVTARADYTYVDAEDRATGLALLRRPKHRATLSAAWTPAERVALSG
SVVHVGGWVDGDRSFTVPRLKADGYTVANLAGEYRLSEAVVLFGRVENLFDERFENPVGFQQPGRGAFAGVRVQWGRGQ

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=606, Percent_Identity=30.03300330033, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI1788478, Length=696, Percent_Identity=27.7298850574713, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786798, Length=723, Percent_Identity=24.4813278008299, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787723, Length=669, Percent_Identity=23.3183856502242, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786344, Length=225, Percent_Identity=28, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68457; Mature: 68326

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCHVPRGSRMNSRILSPAVAAGTLLISGPAWADAATVDDVVVTANRRPTPADAVGSSVT
CEECCCCCCCCCHHHCCCHHHHCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHCCCCEE
VIRRDDIERFQWRSLPEALQLSPGLNVVQTGGTGGRASVFIRGANANHTKVLIDGVDAND
EEECCCHHHHHHHCCCHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCC
PSQGGVFDFGAILTSDIERIEVLRGPQSGLYGSDAIGGVVNIVTRPPGGPLRLRARLEGG
CCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCC
AHETFDQGAGVSGQAGVVGFAFDLAHARTGASDTTPLALLPAGRARHADSYENLTLSGRL
CCHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEC
NARPSARLELDGTVRWSDGDLRFTGDEGFPSAPAAEPSRQDARSLVTRGEARIDAVPDRL
CCCCCCEEEECCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCE
EVRAGVGRADYRTRLQGPGGAAPTYNRGDRTEADLRFDLVLPRGAALVAGIEAERERLKD
EEECCCCCCCHHEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHCC
QPVRYRNDNRAAFAELHLEPAGDLNLTASVRYDDNDRFGGEATWRLAGVYELAATGARLK
CCCEEECCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCEEEEEEEEHHHHHCCCEEE
ASYGTGFKAPSLTQLFVSFPDFGFFANPDLDPETSRGFDVGFEQPLGGRVEVGATYFRQT
ECCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHH
IRNLIQSNAAFTSYENVGRARAEGVEAYAALRLSAAVTARADYTYVDAEDRATGLALLRR
HHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHEEHEEEEECCCEEEECCCCCHHHHHHHCC
PKHRATLSAAWTPAERVALSGSVVHVGGWVDGDRSFTVPRLKADGYTVANLAGEYRLSEA
CCHHEEEEECCCCHHHEEECCCEEEECCEECCCCCEECCEEECCCEEEECCCCCHHHCCC
VVLFGRVENLFDERFENPVGFQQPGRGAFAGVRVQWGRGQ
EEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
TCHVPRGSRMNSRILSPAVAAGTLLISGPAWADAATVDDVVVTANRRPTPADAVGSSVT
EECCCCCCCCCHHHCCCHHHHCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHCCCCEE
VIRRDDIERFQWRSLPEALQLSPGLNVVQTGGTGGRASVFIRGANANHTKVLIDGVDAND
EEECCCHHHHHHHCCCHHHHCCCCCCEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCC
PSQGGVFDFGAILTSDIERIEVLRGPQSGLYGSDAIGGVVNIVTRPPGGPLRLRARLEGG
CCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCC
AHETFDQGAGVSGQAGVVGFAFDLAHARTGASDTTPLALLPAGRARHADSYENLTLSGRL
CCHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEC
NARPSARLELDGTVRWSDGDLRFTGDEGFPSAPAAEPSRQDARSLVTRGEARIDAVPDRL
CCCCCCEEEECCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCCCE
EVRAGVGRADYRTRLQGPGGAAPTYNRGDRTEADLRFDLVLPRGAALVAGIEAERERLKD
EEECCCCCCCHHEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHCC
QPVRYRNDNRAAFAELHLEPAGDLNLTASVRYDDNDRFGGEATWRLAGVYELAATGARLK
CCCEEECCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCEEEEEEEEHHHHHCCCEEE
ASYGTGFKAPSLTQLFVSFPDFGFFANPDLDPETSRGFDVGFEQPLGGRVEVGATYFRQT
ECCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHH
IRNLIQSNAAFTSYENVGRARAEGVEAYAALRLSAAVTARADYTYVDAEDRATGLALLRR
HHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHEEHEEEEECCCEEEECCCCCHHHHHHHCC
PKHRATLSAAWTPAERVALSGSVVHVGGWVDGDRSFTVPRLKADGYTVANLAGEYRLSEA
CCHHEEEEECCCCHHHEEECCCEEEECCEECCCCCEECCEEECCCEEEECCCCCHHHCCC
VVLFGRVENLFDERFENPVGFQQPGRGAFAGVRVQWGRGQ
EEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA