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The map label for this gene is trmH [C]

Identifier: 197105361

GI number: 197105361

Start: 2123705

End: 2124490

Strand: Direct

Name: trmH [C]

Synonym: PHZ_c1898

Alternate gene names: 197105361

Gene position: 2123705-2124490 (Clockwise)

Preceding gene: 197105360

Following gene: 197105362

Centisome position: 53.14

GC content: 71.63

Gene sequence:

>786_bases
ATGAGGGCCGTCACCTCGCTGACCAACCCCACGGTCAAGGCGGTCCGCGCTCTTCACCTGCGCAAGGACCGCGACGAGAC
CGGCCTGTTCCTGGCCGAGGGCCTGAAGCACGTCACCGAGGGCGTCGAGCTGGGCCACGCGCCGCGCATCCTGATGTACG
GCCCGGAGGCGGCCAGCCATCCGCTGCTGCGCAAGGCGGCGGACGCGACGCGGCGGGCCGGCGGCGAGGTCATCGAGGTG
ACCCAGGAGATCCTGGCCAAGGTGTCCCGGCGCGACAATCCGCAAGCGGTGGTCGGCGTGTTCCCGCAGGTCTACCGGCC
GCTGTCCGAGATCGACCCGAAGGCCGCCGACTGCTGGGTGGCGCTGCACCGAGTGCGCGATCCGGGCAACCTGGGGACCA
TCGTGCGCACCGCGGACGCGGCGGGCTGCGGCGGGGTGATCCTGGTGGGCGAGTGCTGCGATCCCTATTCGGTCGAGGCG
GTGCGCGCGACGATGGGCTCGGTGTTCGCCGTGCCGCTCAGCCGCTGCGGCGAGGCCGAGTTCGCGGCCTGGCGGAAGGG
CTGGCCGGGCTCGGTGGTGGGCACCCTGCTGAGCGCCACGCACAGCCACGCGAGCGCCGACTACCGCAAGCCCGCCCTGA
TCTTGATGGGCAACGAGCAGCAGGGGCTGACGCCGGAGATGGCCGCCCTGCCCGACCTGAACGTGAAGATTCCCATGCGC
GGCCGGGCCGACAGCCTGAACCTGTCGGTGGCGACGGGGATCATGATCTACGCTGCGACCAGCTGA

Upstream 100 bases:

>100_bases
TCGCGGGCCGCGGCGGGCGGATCGACTGGGGCGAGCTGGCGCTGGTGGAGGACGGCGGGGATCGGCAGGTCGGCATGAGC
TTCTACGGGCGCTGGTCGCG

Downstream 100 bases:

>100_bases
GGCGTGTCACAAGGGCGGGGGACCGCACGTCCTTGCGACGTATCGAGAGAAGGACCCCTCCCCCATGACCACCCGCCTGA
ACTATGTCGCCGTCGCCCCG

Product: rRNA methylase protein

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRAVTSLTNPTVKAVRALHLRKDRDETGLFLAEGLKHVTEGVELGHAPRILMYGPEAASHPLLRKAADATRRAGGEVIEV
TQEILAKVSRRDNPQAVVGVFPQVYRPLSEIDPKAADCWVALHRVRDPGNLGTIVRTADAAGCGGVILVGECCDPYSVEA
VRATMGSVFAVPLSRCGEAEFAAWRKGWPGSVVGTLLSATHSHASADYRKPALILMGNEQQGLTPEMAALPDLNVKIPMR
GRADSLNLSVATGIMIYAATS

Sequences:

>Translated_261_residues
MRAVTSLTNPTVKAVRALHLRKDRDETGLFLAEGLKHVTEGVELGHAPRILMYGPEAASHPLLRKAADATRRAGGEVIEV
TQEILAKVSRRDNPQAVVGVFPQVYRPLSEIDPKAADCWVALHRVRDPGNLGTIVRTADAAGCGGVILVGECCDPYSVEA
VRATMGSVFAVPLSRCGEAEFAAWRKGWPGSVVGTLLSATHSHASADYRKPALILMGNEQQGLTPEMAALPDLNVKIPMR
GRADSLNLSVATGIMIYAATS
>Mature_261_residues
MRAVTSLTNPTVKAVRALHLRKDRDETGLFLAEGLKHVTEGVELGHAPRILMYGPEAASHPLLRKAADATRRAGGEVIEV
TQEILAKVSRRDNPQAVVGVFPQVYRPLSEIDPKAADCWVALHRVRDPGNLGTIVRTADAAGCGGVILVGECCDPYSVEA
VRATMGSVFAVPLSRCGEAEFAAWRKGWPGSVVGTLLSATHSHASADYRKPALILMGNEQQGLTPEMAALPDLNVKIPMR
GRADSLNLSVATGIMIYAATS

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=165, Percent_Identity=27.8787878787879, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1790083, Length=145, Percent_Identity=35.8620689655172, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24666840, Length=277, Percent_Identity=22.7436823104693, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 27824; Mature: 27824

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVTSLTNPTVKAVRALHLRKDRDETGLFLAEGLKHVTEGVELGHAPRILMYGPEAASH
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCC
PLLRKAADATRRAGGEVIEVTQEILAKVSRRDNPQAVVGVFPQVYRPLSEIDPKAADCWV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHCCHHHCCCCHHHHHH
ALHRVRDPGNLGTIVRTADAAGCGGVILVGECCDPYSVEAVRATMGSVFAVPLSRCGEAE
HHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
FAAWRKGWPGSVVGTLLSATHSHASADYRKPALILMGNEQQGLTPEMAALPDLNVKIPMR
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEECC
GRADSLNLSVATGIMIYAATS
CCCCCCCEEEECCEEEEEECC
>Mature Secondary Structure
MRAVTSLTNPTVKAVRALHLRKDRDETGLFLAEGLKHVTEGVELGHAPRILMYGPEAASH
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCC
PLLRKAADATRRAGGEVIEVTQEILAKVSRRDNPQAVVGVFPQVYRPLSEIDPKAADCWV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHCCHHHCCCCHHHHHH
ALHRVRDPGNLGTIVRTADAAGCGGVILVGECCDPYSVEAVRATMGSVFAVPLSRCGEAE
HHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
FAAWRKGWPGSVVGTLLSATHSHASADYRKPALILMGNEQQGLTPEMAALPDLNVKIPMR
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEECC
GRADSLNLSVATGIMIYAATS
CCCCCCCEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]