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The map label for this gene is mdeA [H]

Identifier: 197105294

GI number: 197105294

Start: 2049486

End: 2050784

Strand: Reverse

Name: mdeA [H]

Synonym: PHZ_c1831

Alternate gene names: 197105294

Gene position: 2050784-2049486 (Counterclockwise)

Preceding gene: 197105295

Following gene: 197105293

Centisome position: 51.32

GC content: 70.82

Gene sequence:

>1299_bases
ATGTCCGACAAGACCCTTCGGCCCGAGACCCTGGCCGTCCACGCCGGCTGGCGGGCGGACCCGACCACAGGCTCGGTGGC
CGTGCCGATCCACCAGACCACCTCCTACCAGTTCCAGAGCGCCGAACACGCCGCGTCCCTGTTCGCCCTCCAGGAGATGG
GCAACATCTACACGCGGATCATGAACCCCACGACCGACGTGCTGGAGCAGCGCGTCGCCGCCCTCGAGGGCGGGGTGGGG
GCCCTCGCGGTCGCCTCGGGCCAGGCCGCCTCGGCGTTCGCGCTGCAGAACCTGGCGCGCGCGGGCGACAACGTGGTCAG
CTCGACCTCGCTCTACGGCGGCACCTGGAACCTGTTCGCCAACACCCTGAAGGACCAGGGGATCGAGGTGCGGTTCGTCG
ATCCCGCCGACCCCGAGAACTTCCGCCGGGCCACCGACGAGCGGACCCGCGCCTACTATGGCGAGACCCTGCCGAACCCG
AAGCTGGAGGTCTTCCCGATCGCCGAGGTGGCCGCCATCGGCCGCGAATTCGGCGTGCCGCTGATCATGGACAACACGGC
CGCGCCGCTGCTGTGCCGTCCGCTGGAGCACGGCGCGGCGATCGTCGTCTACTCGGCCACCAAGTACCTGGGCGGCCACG
GCACCTCGATCGGCGGGCTGATCGTCGACGGCGGAAACTTCGACTGGGAGTCCAGGCCCGAGCGCCAGCCGCTGCTGAAC
ACGCCCGACCCGTCCTACCACGGCGCGGTGTGGACCGAGGCGGTCAAGCCGCTGGGGCCGATCGCCTATATCCTGCGGGC
GCGGGTCATCCTGCTGCGCGACCTCGGCGCGGCCCTCTCGCCGTTCAACGCCTTCCAGCTGATCCAGGGCGTCGAGACCG
TGGCGCTACGCATGGAGCGCCACTGCGCCAACACCCAGCGGGTGGCCGACTACCTGGCCGGCCGCAAGGGCGTGACCCGC
GTCATCCATCCCTCGCAGCAGTCCGGAGAGGCGCGGGCCAGGGCCGACCGGTACCTGAAGGGCGGCTACGGCGGCCTGCT
GGGTTTCGAGCTGGAAGGCGGCGTCGAGGCGGGGCGGAAGTTCATCGACGCGCTGCAGCTCTTCTACCACGTGGCCAACA
TCGGCGACGCGCGCAGCCTGGCGATCCACCCGGCTTCGACCACCCACTCCCAGCTCACCGCCGAGGAGCAGGCGGCCGCG
GGGGTCTCGCCCGGCTATGTGCGCCTGTCGATCGGCATCGAGCATATCGACGACATCCTGGCCGACCTGGACCAGGCGCT
GAAGGCGGCCGGCGCCTAG

Upstream 100 bases:

>100_bases
GTGGCCTTCACCAAGGTCCTCGAAGCCTGGCGCGACGAAGGCGAGCTGAAGGGCCTGGAGCTGACGGCCTGAGGGCCCAG
CGAGACGAGTAGGAGTGACG

Downstream 100 bases:

>100_bases
GCCTGCACGGTTTCTGCAAGGCAGGACCGCGGGCGCTGCACGGCGCCTCCCGCAGGGACTGACAGCGCTCGGTTACCCCC
TGGAGCGAGGCTTCCAGGGG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSDKTLRPETLAVHAGWRADPTTGSVAVPIHQTTSYQFQSAEHAASLFALQEMGNIYTRIMNPTTDVLEQRVAALEGGVG
ALAVASGQAASAFALQNLARAGDNVVSSTSLYGGTWNLFANTLKDQGIEVRFVDPADPENFRRATDERTRAYYGETLPNP
KLEVFPIAEVAAIGREFGVPLIMDNTAAPLLCRPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWESRPERQPLLN
TPDPSYHGAVWTEAVKPLGPIAYILRARVILLRDLGAALSPFNAFQLIQGVETVALRMERHCANTQRVADYLAGRKGVTR
VIHPSQQSGEARARADRYLKGGYGGLLGFELEGGVEAGRKFIDALQLFYHVANIGDARSLAIHPASTTHSQLTAEEQAAA
GVSPGYVRLSIGIEHIDDILADLDQALKAAGA

Sequences:

>Translated_432_residues
MSDKTLRPETLAVHAGWRADPTTGSVAVPIHQTTSYQFQSAEHAASLFALQEMGNIYTRIMNPTTDVLEQRVAALEGGVG
ALAVASGQAASAFALQNLARAGDNVVSSTSLYGGTWNLFANTLKDQGIEVRFVDPADPENFRRATDERTRAYYGETLPNP
KLEVFPIAEVAAIGREFGVPLIMDNTAAPLLCRPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWESRPERQPLLN
TPDPSYHGAVWTEAVKPLGPIAYILRARVILLRDLGAALSPFNAFQLIQGVETVALRMERHCANTQRVADYLAGRKGVTR
VIHPSQQSGEARARADRYLKGGYGGLLGFELEGGVEAGRKFIDALQLFYHVANIGDARSLAIHPASTTHSQLTAEEQAAA
GVSPGYVRLSIGIEHIDDILADLDQALKAAGA
>Mature_431_residues
SDKTLRPETLAVHAGWRADPTTGSVAVPIHQTTSYQFQSAEHAASLFALQEMGNIYTRIMNPTTDVLEQRVAALEGGVGA
LAVASGQAASAFALQNLARAGDNVVSSTSLYGGTWNLFANTLKDQGIEVRFVDPADPENFRRATDERTRAYYGETLPNPK
LEVFPIAEVAAIGREFGVPLIMDNTAAPLLCRPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWESRPERQPLLNT
PDPSYHGAVWTEAVKPLGPIAYILRARVILLRDLGAALSPFNAFQLIQGVETVALRMERHCANTQRVADYLAGRKGVTRV
IHPSQQSGEARARADRYLKGGYGGLLGFELEGGVEAGRKFIDALQLFYHVANIGDARSLAIHPASTTHSQLTAEEQAAAG
VSPGYVRLSIGIEHIDDILADLDQALKAAGA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=426, Percent_Identity=32.8638497652582, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI25453487, Length=424, Percent_Identity=29.7169811320755, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI299473759, Length=426, Percent_Identity=30.0469483568075, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1790375, Length=427, Percent_Identity=31.1475409836066, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1789383, Length=433, Percent_Identity=26.3279445727483, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71980708, Length=422, Percent_Identity=27.7251184834123, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=30.6791569086651, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560056, Length=378, Percent_Identity=32.5396825396825, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=45.2054794520548, Blast_Score=340, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6319307, Length=432, Percent_Identity=31.9444444444444, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321254, Length=453, Percent_Identity=24.7240618101545, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321170, Length=357, Percent_Identity=25.7703081232493, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=29.9765807962529, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46264; Mature: 46133

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKTLRPETLAVHAGWRADPTTGSVAVPIHQTTSYQFQSAEHAASLFALQEMGNIYTRI
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MNPTTDVLEQRVAALEGGVGALAVASGQAASAFALQNLARAGDNVVSSTSLYGGTWNLFA
CCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
NTLKDQGIEVRFVDPADPENFRRATDERTRAYYGETLPNPKLEVFPIAEVAAIGREFGVP
HHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCC
LIMDNTAAPLLCRPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWESRPERQPLLN
EEECCCCCCCEEEECCCCCEEEEEECHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TPDPSYHGAVWTEAVKPLGPIAYILRARVILLRDLGAALSPFNAFQLIQGVETVALRMER
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HCANTQRVADYLAGRKGVTRVIHPSQQSGEARARADRYLKGGYGGLLGFELEGGVEAGRK
HHCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
FIDALQLFYHVANIGDARSLAIHPASTTHSQLTAEEQAAAGVSPGYVRLSIGIEHIDDIL
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
ADLDQALKAAGA
HHHHHHHHHCCC
>Mature Secondary Structure 
SDKTLRPETLAVHAGWRADPTTGSVAVPIHQTTSYQFQSAEHAASLFALQEMGNIYTRI
CCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MNPTTDVLEQRVAALEGGVGALAVASGQAASAFALQNLARAGDNVVSSTSLYGGTWNLFA
CCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
NTLKDQGIEVRFVDPADPENFRRATDERTRAYYGETLPNPKLEVFPIAEVAAIGREFGVP
HHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCC
LIMDNTAAPLLCRPLEHGAAIVVYSATKYLGGHGTSIGGLIVDGGNFDWESRPERQPLLN
EEECCCCCCCEEEECCCCCEEEEEECHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
TPDPSYHGAVWTEAVKPLGPIAYILRARVILLRDLGAALSPFNAFQLIQGVETVALRMER
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HCANTQRVADYLAGRKGVTRVIHPSQQSGEARARADRYLKGGYGGLLGFELEGGVEAGRK
HHCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
FIDALQLFYHVANIGDARSLAIHPASTTHSQLTAEEQAAAGVSPGYVRLSIGIEHIDDIL
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
ADLDQALKAAGA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]