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The map label for this gene is otsA [H]

Identifier: 197105279

GI number: 197105279

Start: 2033863

End: 2035263

Strand: Reverse

Name: otsA [H]

Synonym: PHZ_c1816

Alternate gene names: 197105279

Gene position: 2035263-2033863 (Counterclockwise)

Preceding gene: 197105280

Following gene: 197105277

Centisome position: 50.93

GC content: 69.74

Gene sequence:

>1401_bases
ATGGGACGCCTGATCGTCGTCTCCAACCGGGTGGGTCAGCCGGACCAGAGCGGAGGCCCGTCCGTCGGCGGCCTGGCGAT
GGCGCTCGGCGCCGCCCTTCGCGAATACAACGGCGTCTGGTTCGGCTGGAGCGGCCGGACCGTCGAGACGTTCACCGGCG
GCCTGTCTGTCCAGCGCGGGCAGGGCGCTACCGTCGCGACGGTGGATCTCGAGGAGCAGGACCGGCAGGAATACTACAAC
GGCTACGCAAACCGTACGCTGTGGCCGCTGTTCCACTACCGGATCGACCTGTCCGCCTACGAGCGCTCGTTCAACGAAGG
CTATGCGCGGGTGAACGCCCGCTTCGCCCAGACCCTGCTGCCGCTGCTGAAGCCGGACGACTTCATCTGGGTCCACGACT
ACCACCTGATCCCGCTTGGGCGGGAACTCCGCCGACGCGGGGTGAAGAACCGGATCGGCTTCTTCCTGCACATCCCCTGG
CCGGCGCGCCGGCTGTTCGCGACCCTGCCGTTCCATCGCGAGCTGGTGGAGGCGGTTTTCGACTACGACCTCGTGGGCTT
CCAGAGCGAGGATTCCCTCAGCGCCTTCGAGGACTATGTCGAGAGGGAAGCCGGCGGGACCGTCGGGCCGAACGGACGCT
TCGAGGCCTTCGGCCGAAGCCTCCAGGCCGGCGTGTTTCCGATCGGTCTCGACACCAAGGCGTTCCTCGAGGGAGCGGCG
ACCGAGGCGGCCTCGGCCTGGCGGGACCGGATGCGGGTCAGCCTCGCCGGGCGGCGCATGATCATCGGCGTCGACCGGCT
GGACTACTCCAAGGGGCTGGAGGAGCGCTTCCTCGCCTACGAGGAGTATCTCTCCCACGCGCCCGAGGCGCTGGAGCAGG
TGTTCCTGCTGCAGATCGCCACGCCGAGCCGTGACGAGGTGGCCGCCTATCAGGAGATCCGCGCGCGCCTGGACGCGGTC
ACCGGCCGCATCAATGGCGCCTTCGCAACGCCCGACTGGGTGCCGATCCGCTACGTGAACCGCAGCTACCGCCGAGACGA
GCTGGCCGGCATCTACGGCGTCGCGGCGATCGGCCTCGTCACCCCGCTGCGCGACGGGATGAACCTGGTGGCCAAGGAGT
ATGTGGCGGCCCAGCCGCCCGAGGATCCGGGCGTGCTGATCCTCTCGCGGTTCGCCGGCGCCGCCGAGCAGATGGGCGAG
GCGCTGATCGTCAATCCCTTCAGCCGGGAAGAGGTCTCAGACGCGATCCGGGAGGGGCTGGCCATGCCCCTGAAGGAACG
CGTCCGCCGTTGGGAGGCGCTGATGGCCGGCGTGCGGCGGGACGACGTCGACGCCTGGCGCGACGCCTTCGTCTCGGCCT
TGCGGGCGGTCCCGCAGCGCGAGCTGCCGCCGGCCGCCTAG

Upstream 100 bases:

>100_bases
GGCGCTGAGCTGGCCCGCCGGCTCGAGGACACCTGGGGCGTGCCTTCCGTGGTGGTGGCGCCCGCGGCCTGACCCGAAGG
CGACGCCCAAGGGGAGTGGC

Downstream 100 bases:

>100_bases
GGTCGGCCCTTGAACACCCGGAAGCCCGCCTCGTCGGCGATCGCGAGCATCTCGGTGTCGCCCGAGGTGTCGCCGTAGGC
CGCCGCGAGCCGCAGGTCAG

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGRLIVVSNRVGQPDQSGGPSVGGLAMALGAALREYNGVWFGWSGRTVETFTGGLSVQRGQGATVATVDLEEQDRQEYYN
GYANRTLWPLFHYRIDLSAYERSFNEGYARVNARFAQTLLPLLKPDDFIWVHDYHLIPLGRELRRRGVKNRIGFFLHIPW
PARRLFATLPFHRELVEAVFDYDLVGFQSEDSLSAFEDYVEREAGGTVGPNGRFEAFGRSLQAGVFPIGLDTKAFLEGAA
TEAASAWRDRMRVSLAGRRMIIGVDRLDYSKGLEERFLAYEEYLSHAPEALEQVFLLQIATPSRDEVAAYQEIRARLDAV
TGRINGAFATPDWVPIRYVNRSYRRDELAGIYGVAAIGLVTPLRDGMNLVAKEYVAAQPPEDPGVLILSRFAGAAEQMGE
ALIVNPFSREEVSDAIREGLAMPLKERVRRWEALMAGVRRDDVDAWRDAFVSALRAVPQRELPPAA

Sequences:

>Translated_466_residues
MGRLIVVSNRVGQPDQSGGPSVGGLAMALGAALREYNGVWFGWSGRTVETFTGGLSVQRGQGATVATVDLEEQDRQEYYN
GYANRTLWPLFHYRIDLSAYERSFNEGYARVNARFAQTLLPLLKPDDFIWVHDYHLIPLGRELRRRGVKNRIGFFLHIPW
PARRLFATLPFHRELVEAVFDYDLVGFQSEDSLSAFEDYVEREAGGTVGPNGRFEAFGRSLQAGVFPIGLDTKAFLEGAA
TEAASAWRDRMRVSLAGRRMIIGVDRLDYSKGLEERFLAYEEYLSHAPEALEQVFLLQIATPSRDEVAAYQEIRARLDAV
TGRINGAFATPDWVPIRYVNRSYRRDELAGIYGVAAIGLVTPLRDGMNLVAKEYVAAQPPEDPGVLILSRFAGAAEQMGE
ALIVNPFSREEVSDAIREGLAMPLKERVRRWEALMAGVRRDDVDAWRDAFVSALRAVPQRELPPAA
>Mature_465_residues
GRLIVVSNRVGQPDQSGGPSVGGLAMALGAALREYNGVWFGWSGRTVETFTGGLSVQRGQGATVATVDLEEQDRQEYYNG
YANRTLWPLFHYRIDLSAYERSFNEGYARVNARFAQTLLPLLKPDDFIWVHDYHLIPLGRELRRRGVKNRIGFFLHIPWP
ARRLFATLPFHRELVEAVFDYDLVGFQSEDSLSAFEDYVEREAGGTVGPNGRFEAFGRSLQAGVFPIGLDTKAFLEGAAT
EAASAWRDRMRVSLAGRRMIIGVDRLDYSKGLEERFLAYEEYLSHAPEALEQVFLLQIATPSRDEVAAYQEIRARLDAVT
GRINGAFATPDWVPIRYVNRSYRRDELAGIYGVAAIGLVTPLRDGMNLVAKEYVAAQPPEDPGVLILSRFAGAAEQMGEA
LIVNPFSREEVSDAIREGLAMPLKERVRRWEALMAGVRRDDVDAWRDAFVSALRAVPQRELPPAA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=460, Percent_Identity=46.0869565217391, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71987012, Length=421, Percent_Identity=28.5035629453682, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI115533558, Length=422, Percent_Identity=28.9099526066351, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI115533560, Length=422, Percent_Identity=28.9099526066351, Blast_Score=157, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6319602, Length=467, Percent_Identity=35.3319057815846, Blast_Score=299, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6323537, Length=376, Percent_Identity=28.7234042553192, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323917, Length=373, Percent_Identity=27.6139410187668, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320279, Length=400, Percent_Identity=26.75, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI19920676, Length=496, Percent_Identity=37.0967741935484, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52135; Mature: 52003

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLIVVSNRVGQPDQSGGPSVGGLAMALGAALREYNGVWFGWSGRTVETFTGGLSVQRG
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHEECCCCEEECC
QGATVATVDLEEQDRQEYYNGYANRTLWPLFHYRIDLSAYERSFNEGYARVNARFAQTLL
CCCEEEEEECCHHHHHHHHCCCCCCEEEEHHHEEECHHHHHHHHHHHHHHHHHHHHHHHH
PLLKPDDFIWVHDYHLIPLGRELRRRGVKNRIGFFLHIPWPARRLFATLPFHRELVEAVF
HHHCCCCEEEEEEEEEECCCHHHHHCCCHHHCCEEEECCCHHHHHHHHCCHHHHHHHHHH
DYDLVGFQSEDSLSAFEDYVEREAGGTVGPNGRFEAFGRSLQAGVFPIGLDTKAFLEGAA
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHCCEEEECCCHHHHHHHHH
TEAASAWRDRMRVSLAGRRMIIGVDRLDYSKGLEERFLAYEEYLSHAPEALEQVFLLQIA
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEC
TPSRDEVAAYQEIRARLDAVTGRINGAFATPDWVPIRYVNRSYRRDELAGIYGVAAIGLV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHH
TPLRDGMNLVAKEYVAAQPPEDPGVLILSRFAGAAEQMGEALIVNPFSREEVSDAIREGL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHCCCEEEECCCCHHHHHHHHHHCC
AMPLKERVRRWEALMAGVRRDDVDAWRDAFVSALRAVPQRELPPAA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GRLIVVSNRVGQPDQSGGPSVGGLAMALGAALREYNGVWFGWSGRTVETFTGGLSVQRG
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHEECCCCEEECC
QGATVATVDLEEQDRQEYYNGYANRTLWPLFHYRIDLSAYERSFNEGYARVNARFAQTLL
CCCEEEEEECCHHHHHHHHCCCCCCEEEEHHHEEECHHHHHHHHHHHHHHHHHHHHHHHH
PLLKPDDFIWVHDYHLIPLGRELRRRGVKNRIGFFLHIPWPARRLFATLPFHRELVEAVF
HHHCCCCEEEEEEEEEECCCHHHHHCCCHHHCCEEEECCCHHHHHHHHCCHHHHHHHHHH
DYDLVGFQSEDSLSAFEDYVEREAGGTVGPNGRFEAFGRSLQAGVFPIGLDTKAFLEGAA
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHCCEEEECCCHHHHHHHHH
TEAASAWRDRMRVSLAGRRMIIGVDRLDYSKGLEERFLAYEEYLSHAPEALEQVFLLQIA
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEC
TPSRDEVAAYQEIRARLDAVTGRINGAFATPDWVPIRYVNRSYRRDELAGIYGVAAIGLV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHH
TPLRDGMNLVAKEYVAAQPPEDPGVLILSRFAGAAEQMGEALIVNPFSREEVSDAIREGL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHCCCEEEECCCCHHHHHHHHHHCC
AMPLKERVRRWEALMAGVRRDDVDAWRDAFVSALRAVPQRELPPAA
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA