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The map label for this gene is proS

Identifier: 197105248

GI number: 197105248

Start: 1999241

End: 2000560

Strand: Reverse

Name: proS

Synonym: PHZ_c1785

Alternate gene names: 197105248

Gene position: 2000560-1999241 (Counterclockwise)

Preceding gene: 197105249

Following gene: 197105247

Centisome position: 50.06

GC content: 69.32

Gene sequence:

>1320_bases
ATGCGCCTTTCGCGTTACTTCATGCCGACGTTGCGCGAGGCGCCCTCGGACGCCCAGATCGTCTCGCACCAGCTGATGCT
CCGCGCCGGCATGATCCGGCAGGAGGCGGCCGGCATCTACGCCTGGCTGCCGCTGGGCCTGAAGGTGCTGAAGAAGATCG
AGCAGGTCGTCCGCGAGGAGATGGACCGGGCCGGCGCCATCGAGGTGCTGATGCCGACGCTCCAGCTCGCCGACCTGTGG
CGCGAGAGCGGCCGCTACGACGACTACGGCGAGGAGATGCTGCGCATCAAGGACCGCCACGAGCGGGACCTGCTGTACGG
CCCCACGGCGGAGGAGGTCATCACCGACATCTTCCGGGCCTACATCAAGTCCTACAAGGACTTGCCGAAGAATCTTTACA
ACATCCAGTGGAAGTTCCGCGACGAGCGCCGGCCCCGGTTCGGGGTCATGCGCGGCCGCGAGTTCCTGATGAAGGACGCC
TATTCCTTCGACATCGACGAGGCCGCCGCACGCCGGGCCTACAACCGGATGTTCTGCGCCTACCTGAACGTCTATGCGCG
GCTGGGGCTGAAGGCGATCCCGGTGCGGGCCGACACCGGCCCGATCGGCGGCGACCTTAGCCACGAGTTCATCATCCTGG
CCGACACGGGCGAGAGCCAGGTCTTCGCCCACCGCGATCTCGTCGAGATGGGCGCGCCGGGCCCGGACATCGACTGGGAC
GGCGATCTTGAGCCGCTGGTCCAGCAGCGCACGCGTCTCTACGCCGCCAGCGACGAGATGCACGATCAGGCCCGTTTCGA
GACCGAGGCGCCCGAGGACAAGCGGATGACCGCCCGCGGCATCGAAGTCGGCCACATCTTCTACTTCGGCGAGAAGTACT
CGCGGCCGATGAACGCCAAGGTCGCCGGGCCCGACGGGGCCGAGCGGTTCGTGCACATGGGCTCGTACGGCGTCGGCGTC
TCGCGCCTGGTGGGCGCGATCATCGAGGCCAGCCACGACGAGGCGGGCATCGTCTGGCCGGACAGCGTGGCGCCGTTCGG
CGCGGCGGTCGTCAACCTGCGGCCCGGCGAGGCGGCGGTGGACGCGGTGGCCGAGCAGGCCTACGGCGCGCTGCAGGCCG
CGGGCAAGGAGCCGCTTCTGGACGACCGTGACGAGCGCCCGGGCGCGAAGTTCGCCTCGCTCGACCTGGTGGGCGTGCCC
TGGCAGCTCATCGTCGGCCCCAAGGGCGTCGCCGAGGGCGTGGTCGAGATCAAGCGCCGGGCCACCGGCGAGCGCCAGAC
CCTGCCGCTGGATGCGGCCCTGAAGGCGATCACCGCTTGA

Upstream 100 bases:

>100_bases
ACTAGGGAGCCGAAACGGCTCAGCTGCGTTGCCAAGCTCGCGGGGGCGGGGCTATCAGAGCGCCTCTTTCCCGCCGTGAA
TCCTTAGGACACCGACCACG

Downstream 100 bases:

>100_bases
GCGACGCCGCCGCCGTCATGGGGGCCAGCGCCCCGCCGTTCGGCTACTGGGAGCGGATGCTCGCAGGCCGCTACCTGCGG
GCCAAGCGCAGCCAGGGCGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRLSRYFMPTLREAPSDAQIVSHQLMLRAGMIRQEAAGIYAWLPLGLKVLKKIEQVVREEMDRAGAIEVLMPTLQLADLW
RESGRYDDYGEEMLRIKDRHERDLLYGPTAEEVITDIFRAYIKSYKDLPKNLYNIQWKFRDERRPRFGVMRGREFLMKDA
YSFDIDEAAARRAYNRMFCAYLNVYARLGLKAIPVRADTGPIGGDLSHEFIILADTGESQVFAHRDLVEMGAPGPDIDWD
GDLEPLVQQRTRLYAASDEMHDQARFETEAPEDKRMTARGIEVGHIFYFGEKYSRPMNAKVAGPDGAERFVHMGSYGVGV
SRLVGAIIEASHDEAGIVWPDSVAPFGAAVVNLRPGEAAVDAVAEQAYGALQAAGKEPLLDDRDERPGAKFASLDLVGVP
WQLIVGPKGVAEGVVEIKRRATGERQTLPLDAALKAITA

Sequences:

>Translated_439_residues
MRLSRYFMPTLREAPSDAQIVSHQLMLRAGMIRQEAAGIYAWLPLGLKVLKKIEQVVREEMDRAGAIEVLMPTLQLADLW
RESGRYDDYGEEMLRIKDRHERDLLYGPTAEEVITDIFRAYIKSYKDLPKNLYNIQWKFRDERRPRFGVMRGREFLMKDA
YSFDIDEAAARRAYNRMFCAYLNVYARLGLKAIPVRADTGPIGGDLSHEFIILADTGESQVFAHRDLVEMGAPGPDIDWD
GDLEPLVQQRTRLYAASDEMHDQARFETEAPEDKRMTARGIEVGHIFYFGEKYSRPMNAKVAGPDGAERFVHMGSYGVGV
SRLVGAIIEASHDEAGIVWPDSVAPFGAAVVNLRPGEAAVDAVAEQAYGALQAAGKEPLLDDRDERPGAKFASLDLVGVP
WQLIVGPKGVAEGVVEIKRRATGERQTLPLDAALKAITA
>Mature_439_residues
MRLSRYFMPTLREAPSDAQIVSHQLMLRAGMIRQEAAGIYAWLPLGLKVLKKIEQVVREEMDRAGAIEVLMPTLQLADLW
RESGRYDDYGEEMLRIKDRHERDLLYGPTAEEVITDIFRAYIKSYKDLPKNLYNIQWKFRDERRPRFGVMRGREFLMKDA
YSFDIDEAAARRAYNRMFCAYLNVYARLGLKAIPVRADTGPIGGDLSHEFIILADTGESQVFAHRDLVEMGAPGPDIDWD
GDLEPLVQQRTRLYAASDEMHDQARFETEAPEDKRMTARGIEVGHIFYFGEKYSRPMNAKVAGPDGAERFVHMGSYGVGV
SRLVGAIIEASHDEAGIVWPDSVAPFGAAVVNLRPGEAAVDAVAEQAYGALQAAGKEPLLDDRDERPGAKFASLDLVGVP
WQLIVGPKGVAEGVVEIKRRATGERQTLPLDAALKAITA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=438, Percent_Identity=34.703196347032, Blast_Score=256, Evalue=3e-68,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=59.7285067873303, Blast_Score=285, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI115532348, Length=401, Percent_Identity=29.426433915212, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=87, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=251, Percent_Identity=33.0677290836653, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24656200, Length=422, Percent_Identity=29.6208530805687, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PHEZH (B4RBZ9)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130625.1
- GeneID:   6791298
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1785
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49101; Mature: 49101

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFMPTLREAPSDAQIVSHQLMLRAGMIRQEAAGIYAWLPLGLKVLKKIEQVVREE
CCCHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MDRAGAIEVLMPTLQLADLWRESGRYDDYGEEMLRIKDRHERDLLYGPTAEEVITDIFRA
HHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHH
YIKSYKDLPKNLYNIQWKFRDERRPRFGVMRGREFLMKDAYSFDIDEAAARRAYNRMFCA
HHHHHHHHCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YLNVYARLGLKAIPVRADTGPIGGDLSHEFIILADTGESQVFAHRDLVEMGAPGPDIDWD
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
GDLEPLVQQRTRLYAASDEMHDQARFETEAPEDKRMTARGIEVGHIFYFGEKYSRPMNAK
CCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEEECHHHCCCCCCE
VAGPDGAERFVHMGSYGVGVSRLVGAIIEASHDEAGIVWPDSVAPFGAAVVNLRPGEAAV
ECCCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEECCCHHHH
DAVAEQAYGALQAAGKEPLLDDRDERPGAKFASLDLVGVPWQLIVGPKGVAEGVVEIKRR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHH
ATGERQTLPLDAALKAITA
CCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MRLSRYFMPTLREAPSDAQIVSHQLMLRAGMIRQEAAGIYAWLPLGLKVLKKIEQVVREE
CCCHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MDRAGAIEVLMPTLQLADLWRESGRYDDYGEEMLRIKDRHERDLLYGPTAEEVITDIFRA
HHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHH
YIKSYKDLPKNLYNIQWKFRDERRPRFGVMRGREFLMKDAYSFDIDEAAARRAYNRMFCA
HHHHHHHHCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YLNVYARLGLKAIPVRADTGPIGGDLSHEFIILADTGESQVFAHRDLVEMGAPGPDIDWD
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
GDLEPLVQQRTRLYAASDEMHDQARFETEAPEDKRMTARGIEVGHIFYFGEKYSRPMNAK
CCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEEECHHHCCCCCCE
VAGPDGAERFVHMGSYGVGVSRLVGAIIEASHDEAGIVWPDSVAPFGAAVVNLRPGEAAV
ECCCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEECCCHHHH
DAVAEQAYGALQAAGKEPLLDDRDERPGAKFASLDLVGVPWQLIVGPKGVAEGVVEIKRR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHH
ATGERQTLPLDAALKAITA
CCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA