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The map label for this gene is gltX

Identifier: 197105227

GI number: 197105227

Start: 1973925

End: 1975331

Strand: Direct

Name: gltX

Synonym: PHZ_c1764

Alternate gene names: 197105227

Gene position: 1973925-1975331 (Clockwise)

Preceding gene: 197105225

Following gene: 197105228

Centisome position: 49.39

GC content: 70.08

Gene sequence:

>1407_bases
ATGCCTGACCGTCCCGTCCGCACCCGGATCGCCCCCTCGCCTACCGGCATGATGCACATTGGCACGGCGAGGACCGCCCT
GTTCAACTGGCTTTATGCCCGCCACACGGGCGGCAAGTTCCTGCTGCGCATCGAGGACACCGACCGGGAGCGTTCGACCG
ACGAGGCGGTGAAGGTGATCTTCGACGGCCTGAAATGGCTGGGCCTGGACGCCGACGAGCCGCCGGTGTTCCAGTTCGCC
CGGGCCGACCGGCACCGCGAGGCCGCCGAGACGCTGCTGGCGCGCGGCGGCGCCTATCGTGATTACATGACGCCCGAGGA
GCTGGAGGCGGAGCGTGAGGTGGCGCGCGCCGAGGGCCGGGTCGTCCGCTCGCCCTGGCGCGACGCCTCGCCCAACGACG
CCCCCGACCGCCCGTTCGTGGTGCGGCTGAAGGCGCCGCAGGAGGGCGAGACTGTCATCCAGGACGCGGTGAAGGGCGAG
GTCCGGTTCCAGAACAAGCAGCTGGACGACCTGATCCTGCTGCGCACCGACGGCACCCCCACCTACAACCTCGCCGTGGT
CGTGGACGACCACGACATGGGCGTGACGCACGTGATCCGCGGCGACGACCACCTGAACAACGCCGCCCGCCAGACGCTGA
TCTATCAAGGCCTCGGCTGGGAGGTCCCGGTGTGGGCGCACCTGCCGCTGATCCACGGCCCCGACGGAGCGAAGCTGTCG
AAGCGCCACGGCGCCCAGGCGGTCAGCGAGTTCGACTCCATGGGCTACCTGCCCGAGACCATGCGCAACTACCTGGCCAA
GCTGGGCTGGGGCCACGGGGACGACGAGATCTTTTCGGATGAGCAGGCGATCGCCTGGTTCGACATCAATGACGTGGTCA
GCGCGCCGGCCCGCCTCGACTGGGCCAAGCTGAACCACCTGAACAACCACTACATCCGCCAGGCCGAACCCGCGCGCCTC
GCCGAGCTGGTCAAGACGGTGCTGGCGAGCCGCGACTGGCCCCTCGAGGCCGGGGACATGGCGGTGATCGAGCGCACGAT
CCCCTTCGTCCGCGACGGCGCCAAGACGACGCTGGAGCTGGCCGACAACGTCGTCTTCGCGCTCAAGCGCCGGCCGCTCG
AGCTGCCCGAGAAGACCCGGACGCAGATGACCGAGGAGCTGCGCGGGCGCCTTTCGCGCCTGCGGGAGGCGCTGGCCGCT
GTCGAATACTGGGACGTGCCGTCGCTCGAGGCGGCGCTGCGGGCGTTCGCGGAAGCCGAAGGCGTGGGCCTGGGCAAGTT
CGGACCGCAGCTGCGCGCCGTGCTTTCCGGCGGCGCGCCGGCCCCCGATCTCGCAGGCGCGATGGTCGCGCTGACCCGCG
ACGAAAGTCTAGGACGGCTCGACGATGCGCTTTCACCGTCCGCGTAA

Upstream 100 bases:

>100_bases
GCGGAGGCTGGAAGCACGCCGAATCCGCATGCTAAGAGGCGCCCCCAAGTGTTCAGCTCCGGCGTTATCCGCCAGGGCCG
CCCCAACCCCTCCGAGATCG

Downstream 100 bases:

>100_bases
ACCGCTATAAGGCCGCTCGAACGAGCGTTTCATCTCGCACCCGCGAAAGGAGTCCGCATGGGCGACACGGCGAAGATCGG
CATCGGCGGCAAGGACGTTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MPDRPVRTRIAPSPTGMMHIGTARTALFNWLYARHTGGKFLLRIEDTDRERSTDEAVKVIFDGLKWLGLDADEPPVFQFA
RADRHREAAETLLARGGAYRDYMTPEELEAEREVARAEGRVVRSPWRDASPNDAPDRPFVVRLKAPQEGETVIQDAVKGE
VRFQNKQLDDLILLRTDGTPTYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQGLGWEVPVWAHLPLIHGPDGAKLS
KRHGAQAVSEFDSMGYLPETMRNYLAKLGWGHGDDEIFSDEQAIAWFDINDVVSAPARLDWAKLNHLNNHYIRQAEPARL
AELVKTVLASRDWPLEAGDMAVIERTIPFVRDGAKTTLELADNVVFALKRRPLELPEKTRTQMTEELRGRLSRLREALAA
VEYWDVPSLEAALRAFAEAEGVGLGKFGPQLRAVLSGGAPAPDLAGAMVALTRDESLGRLDDALSPSA

Sequences:

>Translated_468_residues
MPDRPVRTRIAPSPTGMMHIGTARTALFNWLYARHTGGKFLLRIEDTDRERSTDEAVKVIFDGLKWLGLDADEPPVFQFA
RADRHREAAETLLARGGAYRDYMTPEELEAEREVARAEGRVVRSPWRDASPNDAPDRPFVVRLKAPQEGETVIQDAVKGE
VRFQNKQLDDLILLRTDGTPTYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQGLGWEVPVWAHLPLIHGPDGAKLS
KRHGAQAVSEFDSMGYLPETMRNYLAKLGWGHGDDEIFSDEQAIAWFDINDVVSAPARLDWAKLNHLNNHYIRQAEPARL
AELVKTVLASRDWPLEAGDMAVIERTIPFVRDGAKTTLELADNVVFALKRRPLELPEKTRTQMTEELRGRLSRLREALAA
VEYWDVPSLEAALRAFAEAEGVGLGKFGPQLRAVLSGGAPAPDLAGAMVALTRDESLGRLDDALSPSA
>Mature_467_residues
PDRPVRTRIAPSPTGMMHIGTARTALFNWLYARHTGGKFLLRIEDTDRERSTDEAVKVIFDGLKWLGLDADEPPVFQFAR
ADRHREAAETLLARGGAYRDYMTPEELEAEREVARAEGRVVRSPWRDASPNDAPDRPFVVRLKAPQEGETVIQDAVKGEV
RFQNKQLDDLILLRTDGTPTYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQGLGWEVPVWAHLPLIHGPDGAKLSK
RHGAQAVSEFDSMGYLPETMRNYLAKLGWGHGDDEIFSDEQAIAWFDINDVVSAPARLDWAKLNHLNNHYIRQAEPARLA
ELVKTVLASRDWPLEAGDMAVIERTIPFVRDGAKTTLELADNVVFALKRRPLELPEKTRTQMTEELRGRLSRLREALAAV
EYWDVPSLEAALRAFAEAEGVGLGKFGPQLRAVLSGGAPAPDLAGAMVALTRDESLGRLDDALSPSA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=509, Percent_Identity=30.0589390962672, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI62241042, Length=264, Percent_Identity=26.5151515151515, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=41.8454935622318, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=35.4385964912281, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786895, Length=208, Percent_Identity=25.9615384615385, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=330, Percent_Identity=35.1515151515151, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324540, Length=497, Percent_Identity=31.3883299798793, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=236, Percent_Identity=26.6949152542373, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24665321, Length=485, Percent_Identity=32.1649484536082, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PHEZH (B4RBX8)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130604.1
- GeneID:   6791277
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1764
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52075; Mature: 51944

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRPVRTRIAPSPTGMMHIGTARTALFNWLYARHTGGKFLLRIEDTDRERSTDEAVKVI
CCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHH
FDGLKWLGLDADEPPVFQFARADRHREAAETLLARGGAYRDYMTPEELEAEREVARAEGR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
VVRSPWRDASPNDAPDRPFVVRLKAPQEGETVIQDAVKGEVRFQNKQLDDLILLRTDGTP
EEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCEEEEEECCCC
TYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQGLGWEVPVWAHLPLIHGPDGAKLS
CEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCCCHH
KRHGAQAVSEFDSMGYLPETMRNYLAKLGWGHGDDEIFSDEQAIAWFDINDVVSAPARLD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCC
WAKLNHLNNHYIRQAEPARLAELVKTVLASRDWPLEAGDMAVIERTIPFVRDGAKTTLEL
HHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
ADNVVFALKRRPLELPEKTRTQMTEELRGRLSRLREALAAVEYWDVPSLEAALRAFAEAE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GVGLGKFGPQLRAVLSGGAPAPDLAGAMVALTRDESLGRLDDALSPSA
CCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCC
>Mature Secondary Structure 
PDRPVRTRIAPSPTGMMHIGTARTALFNWLYARHTGGKFLLRIEDTDRERSTDEAVKVI
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHH
FDGLKWLGLDADEPPVFQFARADRHREAAETLLARGGAYRDYMTPEELEAEREVARAEGR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
VVRSPWRDASPNDAPDRPFVVRLKAPQEGETVIQDAVKGEVRFQNKQLDDLILLRTDGTP
EEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCCCCCCEEEEEECCCC
TYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTLIYQGLGWEVPVWAHLPLIHGPDGAKLS
CEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEECCEEECCCCCCHH
KRHGAQAVSEFDSMGYLPETMRNYLAKLGWGHGDDEIFSDEQAIAWFDINDVVSAPARLD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCC
WAKLNHLNNHYIRQAEPARLAELVKTVLASRDWPLEAGDMAVIERTIPFVRDGAKTTLEL
HHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
ADNVVFALKRRPLELPEKTRTQMTEELRGRLSRLREALAAVEYWDVPSLEAALRAFAEAE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GVGLGKFGPQLRAVLSGGAPAPDLAGAMVALTRDESLGRLDDALSPSA
CCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA