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The map label for this gene is ccsA [H]

Identifier: 197105228

GI number: 197105228

Start: 1975389

End: 1976672

Strand: Direct

Name: ccsA [H]

Synonym: PHZ_c1765

Alternate gene names: 197105228

Gene position: 1975389-1976672 (Clockwise)

Preceding gene: 197105227

Following gene: 197105278

Centisome position: 49.43

GC content: 65.97

Gene sequence:

>1284_bases
ATGGGCGACACGGCGAAGATCGGCATCGGCGGCAAGGACGTTGAGCTCCCGGTCCTGAAGGGGACGGTTGGCCCCGACGT
GGTCGACATCCGCAAGCTGTACGCGGAGACCGACACCTTCACCTACGATCCGGGCTTCACGTCGACCGCCTCTTGCGAGA
GCAAGATCACCTTCATCGACGGCGACAAGGGCGTGCTGCTGCACCGCGGCTACCCGATCGACCAGCTGGCGGAGAAGTCG
AGCTTCCTCGAGGTCTGCTACCTGCTGCTGCACGGCGAGCTGCCCACGGCGGCCGAGTTCACGACGTTCGAGCACAACAT
CACCTATCACACGATGGTGCACGAGCAGTTCGACCGCTTCTTCCAGGGCTTCCGCCGGGACGCGCACCCGATGGCGATCA
TGGTCGGCGCGGTCGGGGCCCTCTCGGCCTTCTACCACGACAGCACCAACATCGACGATCCCGAGCAGCGGATCATCTCG
GCTCATCGCATGATCGCCAAGATGCCGACGATCGCGGCGCGGGCGTTCAAGTACTCGAAGGGCCAGCCCTTCATCTATCC
GCGCAACGAGCTCTCGTACGCCGAGAACTTCCTGCGCATGTGCTTCTCGGTCCCGGCCGAGGACTGGAAGCCGAACCCGG
TCCTGACCCGGGCGATGGATCGCATCTTCATCCTGCACGCCGACCACGAGCAGAACGCGTCGACCTCGACCGTCCGCCTC
GCCGGCTCGTCGGGCGCCAATCCGTTCGCGTGCATCGCCGCCGGCATCGCCTGCCTCTGGGGCCCCAGCCACGGCGGGGC
GAACGAAGAGGCGCTGAACATGCTGGCCGAGATCGGCTCGGTGGACCGCATCCCCGAGTACGTCCAGGGCGTGAAGGACC
GCCGCTACCGGCTGATGGGCTTCGGCCACCGGGTCTACAAGAACTACGACCCGCGGGCGAAGGTCATGCAGACCACCTGC
CACGAGGTCCTGGCCGAGGTCGGTCACTCGGACGACCCGCTGCTGAAGGTGGCGATAGAGCTCGAGAAGATCGCGCTCAA
CGATCCCTACTTCATCGAGCGCAAGCTCTACCCGAACGTCGACTTCTACTCGGGCATCACCCTGCGCGCCCTGGGCTTCC
CGCCGGACATGTTCACGGTGCTGTTCGCCCTGGCCCGCACCGTGGGCTGGATCGCGCAGTGGAAGGAAATGATCGAGGAT
CCGTCCCAGAAGATCGGCCGCCCGCGCCAGCTCTACACGGGCGCCGCCCAGCGCGACTACGTGCCGGTGGAGAAGCGGGG
CTAA

Upstream 100 bases:

>100_bases
AAGTCTAGGACGGCTCGACGATGCGCTTTCACCGTCCGCGTAAACCGCTATAAGGCCGCTCGAACGAGCGTTTCATCTCG
CACCCGCGAAAGGAGTCCGC

Downstream 100 bases:

>100_bases
GCAGCCCCCTTCCCTTCCAGGACTTTCAGGACTGCAGACGCGGCGGCCTCGTTCGGATCGGGGCCGCCGCGTCCCATTTT
GTCGAGGGCCGCGTACTGCC

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MGDTAKIGIGGKDVELPVLKGTVGPDVVDIRKLYAETDTFTYDPGFTSTASCESKITFIDGDKGVLLHRGYPIDQLAEKS
SFLEVCYLLLHGELPTAAEFTTFEHNITYHTMVHEQFDRFFQGFRRDAHPMAIMVGAVGALSAFYHDSTNIDDPEQRIIS
AHRMIAKMPTIAARAFKYSKGQPFIYPRNELSYAENFLRMCFSVPAEDWKPNPVLTRAMDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPSHGGANEEALNMLAEIGSVDRIPEYVQGVKDRRYRLMGFGHRVYKNYDPRAKVMQTTC
HEVLAEVGHSDDPLLKVAIELEKIALNDPYFIERKLYPNVDFYSGITLRALGFPPDMFTVLFALARTVGWIAQWKEMIED
PSQKIGRPRQLYTGAAQRDYVPVEKRG

Sequences:

>Translated_427_residues
MGDTAKIGIGGKDVELPVLKGTVGPDVVDIRKLYAETDTFTYDPGFTSTASCESKITFIDGDKGVLLHRGYPIDQLAEKS
SFLEVCYLLLHGELPTAAEFTTFEHNITYHTMVHEQFDRFFQGFRRDAHPMAIMVGAVGALSAFYHDSTNIDDPEQRIIS
AHRMIAKMPTIAARAFKYSKGQPFIYPRNELSYAENFLRMCFSVPAEDWKPNPVLTRAMDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPSHGGANEEALNMLAEIGSVDRIPEYVQGVKDRRYRLMGFGHRVYKNYDPRAKVMQTTC
HEVLAEVGHSDDPLLKVAIELEKIALNDPYFIERKLYPNVDFYSGITLRALGFPPDMFTVLFALARTVGWIAQWKEMIED
PSQKIGRPRQLYTGAAQRDYVPVEKRG
>Mature_426_residues
GDTAKIGIGGKDVELPVLKGTVGPDVVDIRKLYAETDTFTYDPGFTSTASCESKITFIDGDKGVLLHRGYPIDQLAEKSS
FLEVCYLLLHGELPTAAEFTTFEHNITYHTMVHEQFDRFFQGFRRDAHPMAIMVGAVGALSAFYHDSTNIDDPEQRIISA
HRMIAKMPTIAARAFKYSKGQPFIYPRNELSYAENFLRMCFSVPAEDWKPNPVLTRAMDRIFILHADHEQNASTSTVRLA
GSSGANPFACIAAGIACLWGPSHGGANEEALNMLAEIGSVDRIPEYVQGVKDRRYRLMGFGHRVYKNYDPRAKVMQTTCH
EVLAEVGHSDDPLLKVAIELEKIALNDPYFIERKLYPNVDFYSGITLRALGFPPDMFTVLFALARTVGWIAQWKEMIEDP
SQKIGRPRQLYTGAAQRDYVPVEKRG

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=392, Percent_Identity=26.2755102040816, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1786939, Length=430, Percent_Identity=67.4418604651163, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=29.9465240641711, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=381, Percent_Identity=25.7217847769029, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319850, Length=351, Percent_Identity=26.7806267806268, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=329, Percent_Identity=26.1398176291793, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325257, Length=362, Percent_Identity=23.4806629834254, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640126, Length=351, Percent_Identity=27.3504273504274, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640124, Length=351, Percent_Identity=27.3504273504274, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356863, Length=397, Percent_Identity=26.448362720403, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47844; Mature: 47713

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDTAKIGIGGKDVELPVLKGTVGPDVVDIRKLYAETDTFTYDPGFTSTASCESKITFID
CCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEE
GDKGVLLHRGYPIDQLAEKSSFLEVCYLLLHGELPTAAEFTTFEHNITYHTMVHEQFDRF
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
FQGFRRDAHPMAIMVGAVGALSAFYHDSTNIDDPEQRIISAHRMIAKMPTIAARAFKYSK
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQPFIYPRNELSYAENFLRMCFSVPAEDWKPNPVLTRAMDRIFILHADHEQNASTSTVRL
CCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCEEEE
AGSSGANPFACIAAGIACLWGPSHGGANEEALNMLAEIGSVDRIPEYVQGVKDRRYRLMG
ECCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHH
FGHRVYKNYDPRAKVMQTTCHEVLAEVGHSDDPLLKVAIELEKIALNDPYFIERKLYPNV
CCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCCCEEEEECCCCC
DFYSGITLRALGFPPDMFTVLFALARTVGWIAQWKEMIEDPSQKIGRPRQLYTGAAQRDY
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCCC
VPVEKRG
CCCCCCC
>Mature Secondary Structure 
GDTAKIGIGGKDVELPVLKGTVGPDVVDIRKLYAETDTFTYDPGFTSTASCESKITFID
CCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEE
GDKGVLLHRGYPIDQLAEKSSFLEVCYLLLHGELPTAAEFTTFEHNITYHTMVHEQFDRF
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
FQGFRRDAHPMAIMVGAVGALSAFYHDSTNIDDPEQRIISAHRMIAKMPTIAARAFKYSK
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
GQPFIYPRNELSYAENFLRMCFSVPAEDWKPNPVLTRAMDRIFILHADHEQNASTSTVRL
CCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCEEEE
AGSSGANPFACIAAGIACLWGPSHGGANEEALNMLAEIGSVDRIPEYVQGVKDRRYRLMG
ECCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHH
FGHRVYKNYDPRAKVMQTTCHEVLAEVGHSDDPLLKVAIELEKIALNDPYFIERKLYPNV
CCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCCCEEEEECCCCC
DFYSGITLRALGFPPDMFTVLFALARTVGWIAQWKEMIEDPSQKIGRPRQLYTGAAQRDY
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCCC
VPVEKRG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]