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The map label for this gene is pleD [H]

Identifier: 197105175

GI number: 197105175

Start: 1921241

End: 1922290

Strand: Direct

Name: pleD [H]

Synonym: PHZ_c1712

Alternate gene names: 197105175

Gene position: 1921241-1922290 (Clockwise)

Preceding gene: 197105174

Following gene: 197105176

Centisome position: 48.08

GC content: 69.81

Gene sequence:

>1050_bases
ATGTCTTCCGACGTCGAAACATCGCTCCGGGGCCCGAAGGCCTACGCGCTGGCCCGCAAGGCGATCGAGGCCATGGAAGC
GCACCAGGTCTGGCCGACCGCGCTCAACTTCGAGCTCTGGGTGCACTACGTCGCGGCCAAGGATTCCGCCCTGGCCACCG
AGATCGACCATCTGATCGAGGCCGGCGAACCGTTCACCGATGCGGTGGGCGAGCAGCTGGCCGCCCAGTTCCTGCCCAAG
GCCAAGCTGAACGGCGAGATCCTCGAGGCGGGTCAGACCCTGTCGAAGGAACTGGACAGCGTCTCGCGCGCCATCGAATC
CGCCCGCGAGACCTCCGAGGCCTACGGCCAGCAGCTGGCCAGCGCCAGCGAGTCGCTCGACGGCGAGGACGCCCAGGCGA
TCAAGTCGATGGTCGAGACCCTGACTGTCGCCACGCGCAAGGTGCGGGAGGAGAACCATGCGCTGGAAAGCCAGCTGCAG
GACACGACCGCCGAACTCGGCCGCCTGCGCGAGCACCTGGAGCAGGTGCGCCGCGACGCGATGACCGACGCCCTTTCGGG
GCTGGCCAACCGCAAGGCCTTCGACGAGGCGCTGGAGCGGGCCTGCGACCAGGGCGAACAGGGCGGCCCGGTGACCCTGG
CGGTCATCGACATCGACCATTTCAAGAGCTTCAACGACACCTGGGGCCACCAGACGGGCGACCAGGTGATCCGCTACGTA
GCCAGCGTGATCCAGCGCTGCAGCCCCGCCCCGCGGGTTTCGGCGCGCTACGGCGGCGAGGAGTTCGCCATCATCTTCCC
CGGCGAGAGCGCCCGGGACTCGCTGGCGGTCCTGGAGGCTGTGCGCGAGGAGGTCTCGTCCCGCATCCTCAAGCGCCGCT
CGACCAACGAGGACCTCGGGGCGATCACGATCTCGGCCGGCATCGCCGAGCGCAAGCCGGGGGAAAGCCCCGCCAGCCTC
GTCGAGCGCGCCGACAGCGCCCTCTACGCCTCCAAGCGCGGCGGCCGCAACCGCACCTCCTGCGCCGATCCGATCGCCGC
GGCGGCCTGA

Upstream 100 bases:

>100_bases
CCGCGCCCGTCTTAACCGGGCGTTTGCCCGTCCTGGCCTAGGCTTCCCGCCGGCCGATGATGCGTCTCCAGGCTCATCGG
CGGGGAACTGAAGGCGGACC

Downstream 100 bases:

>100_bases
CGCCGCGCGTTTTCCCTTGGCTGACGCCGGGGTATCCTCCGTTCAATCGGCCCGAACGCGAGGGAGGAAGCGATGGACGG
CGCTCAAGCGGCCAGCAAGG

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSSDVETSLRGPKAYALARKAIEAMEAHQVWPTALNFELWVHYVAAKDSALATEIDHLIEAGEPFTDAVGEQLAAQFLPK
AKLNGEILEAGQTLSKELDSVSRAIESARETSEAYGQQLASASESLDGEDAQAIKSMVETLTVATRKVREENHALESQLQ
DTTAELGRLREHLEQVRRDAMTDALSGLANRKAFDEALERACDQGEQGGPVTLAVIDIDHFKSFNDTWGHQTGDQVIRYV
ASVIQRCSPAPRVSARYGGEEFAIIFPGESARDSLAVLEAVREEVSSRILKRRSTNEDLGAITISAGIAERKPGESPASL
VERADSALYASKRGGRNRTSCADPIAAAA

Sequences:

>Translated_349_residues
MSSDVETSLRGPKAYALARKAIEAMEAHQVWPTALNFELWVHYVAAKDSALATEIDHLIEAGEPFTDAVGEQLAAQFLPK
AKLNGEILEAGQTLSKELDSVSRAIESARETSEAYGQQLASASESLDGEDAQAIKSMVETLTVATRKVREENHALESQLQ
DTTAELGRLREHLEQVRRDAMTDALSGLANRKAFDEALERACDQGEQGGPVTLAVIDIDHFKSFNDTWGHQTGDQVIRYV
ASVIQRCSPAPRVSARYGGEEFAIIFPGESARDSLAVLEAVREEVSSRILKRRSTNEDLGAITISAGIAERKPGESPASL
VERADSALYASKRGGRNRTSCADPIAAAA
>Mature_348_residues
SSDVETSLRGPKAYALARKAIEAMEAHQVWPTALNFELWVHYVAAKDSALATEIDHLIEAGEPFTDAVGEQLAAQFLPKA
KLNGEILEAGQTLSKELDSVSRAIESARETSEAYGQQLASASESLDGEDAQAIKSMVETLTVATRKVREENHALESQLQD
TTAELGRLREHLEQVRRDAMTDALSGLANRKAFDEALERACDQGEQGGPVTLAVIDIDHFKSFNDTWGHQTGDQVIRYVA
SVIQRCSPAPRVSARYGGEEFAIIFPGESARDSLAVLEAVREEVSSRILKRRSTNEDLGAITISAGIAERKPGESPASLV
ERADSALYASKRGGRNRTSCADPIAAAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=187, Percent_Identity=34.2245989304813, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=34.4632768361582, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=33.5294117647059, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=33.5227272727273, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787262, Length=193, Percent_Identity=30.5699481865285, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787816, Length=202, Percent_Identity=32.6732673267327, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=35.4651162790698, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=32.7683615819209, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787056, Length=175, Percent_Identity=32, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788381, Length=195, Percent_Identity=32.3076923076923, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081974, Length=178, Percent_Identity=29.7752808988764, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=166, Percent_Identity=30.7228915662651, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=29.7619047619048, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 37749; Mature: 37618

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDVETSLRGPKAYALARKAIEAMEAHQVWPTALNFELWVHYVAAKDSALATEIDHLIE
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AGEPFTDAVGEQLAAQFLPKAKLNGEILEAGQTLSKELDSVSRAIESARETSEAYGQQLA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASESLDGEDAQAIKSMVETLTVATRKVREENHALESQLQDTTAELGRLREHLEQVRRDA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTDALSGLANRKAFDEALERACDQGEQGGPVTLAVIDIDHFKSFNDTWGHQTGDQVIRYV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCCHHCCCCCHHHHHHHH
ASVIQRCSPAPRVSARYGGEEFAIIFPGESARDSLAVLEAVREEVSSRILKRRSTNEDLG
HHHHHHCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AITISAGIAERKPGESPASLVERADSALYASKRGGRNRTSCADPIAAAA
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SSDVETSLRGPKAYALARKAIEAMEAHQVWPTALNFELWVHYVAAKDSALATEIDHLIE
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AGEPFTDAVGEQLAAQFLPKAKLNGEILEAGQTLSKELDSVSRAIESARETSEAYGQQLA
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASESLDGEDAQAIKSMVETLTVATRKVREENHALESQLQDTTAELGRLREHLEQVRRDA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTDALSGLANRKAFDEALERACDQGEQGGPVTLAVIDIDHFKSFNDTWGHQTGDQVIRYV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCCHHCCCCCHHHHHHHH
ASVIQRCSPAPRVSARYGGEEFAIIFPGESARDSLAVLEAVREEVSSRILKRRSTNEDLG
HHHHHHCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AITISAGIAERKPGESPASLVERADSALYASKRGGRNRTSCADPIAAAA
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA