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The map label for this gene is glxR [C]

Identifier: 197105149

GI number: 197105149

Start: 1889329

End: 1890189

Strand: Reverse

Name: glxR [C]

Synonym: PHZ_c1686

Alternate gene names: 197105149

Gene position: 1890189-1889329 (Counterclockwise)

Preceding gene: 197105155

Following gene: 197105148

Centisome position: 47.3

GC content: 72.59

Gene sequence:

>861_bases
ATGAAGGTCGCGTTCGCGGGCCTGGGCGTCATGGGCTTCCCGATGGCGGGGCACCTGGCCAGGGCCGGTCACGAGGTGAG
CGTCTACAACCGCAGTCCGGATAAGGCGCGCCGCTGGGTCGAGGCCCATGGCGGCCGGATGGGCGAGACCGCCGCCGAGG
CCGCGGCGGGGGCCCAGGTGCTGGCCCTCTGCGTCGGCAACGACGACGACGTGCGCCAGGTGGTGGCGCAGGCGCTGCCC
GCCCTGGCCGACGGCGCGGTGATCGTCGACCACACCACCACTTCGGCCGTGGTGGCGCGCGAGATGGCGGCGCTGGCGGC
GCAGAGCGGTCGGGCGTTCGTCGACGCGCCGGTTTCCGGCGGCCAGGCGGGCGCCGAGGCCGGCCAGCTGACCATCATGT
GCGGCGGGGACGAAGCCGCCTTCGCCAAGGTCGAGCCGGTGATCGCCGCCTACGCCAAGGCCATCCGCCGGATGGGCGGG
CCGGGCGCGGGCCAGCTGACCAAGATGGTCAACCAGATCTGCATCGCCGGCGTGGTCCAGGGCCTCGCCGAGGGGCTGCA
CTTCGCAAGGCGCGCCGGGCTCGACCCGCAGGCGGTCTACGAGGCGATCTCGAAGGGCGCGGCCCAGTCCTGGCAGATGG
ACAACCGCTGGAAGACCATGAGCGAGGGCAGGTTCGACTTCGGCTTTGCGGTGGACTGGATGCGCAAGGACCTCGGGCTC
CTGCTCGACGAGGCGACCCGCAACGGCGCGACGCTGGAGATGACCGCCATGGTCGACCGGTTCTACGCCGAGGTGCAGGC
CCTGGGGGGCGCCCGTTGGGACACCTCGAGCCTCGTCGCGCGGCTGGAGAAGCCGGAATGA

Upstream 100 bases:

>100_bases
GGTCGCCCACCGACATGGCTCACCCCAGGTTGGCCTGTTACAGGGGGCCAGGGTCGTCGCTGCTCGTTAAGATGCAGCTA
AGCTTACGGAGTTCGAGGGG

Downstream 100 bases:

>100_bases
TCCTCAGGACCGAGCGTCTGACGCTGACGCCCCCGACCGAGGCCGACGCGGCCTTCGTCCTGGAGCTGCTGAACGATCCG
GGCTGGATCGCCAACATCGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKVAFAGLGVMGFPMAGHLARAGHEVSVYNRSPDKARRWVEAHGGRMGETAAEAAAGAQVLALCVGNDDDVRQVVAQALP
ALADGAVIVDHTTTSAVVAREMAALAAQSGRAFVDAPVSGGQAGAEAGQLTIMCGGDEAAFAKVEPVIAAYAKAIRRMGG
PGAGQLTKMVNQICIAGVVQGLAEGLHFARRAGLDPQAVYEAISKGAAQSWQMDNRWKTMSEGRFDFGFAVDWMRKDLGL
LLDEATRNGATLEMTAMVDRFYAEVQALGGARWDTSSLVARLEKPE

Sequences:

>Translated_286_residues
MKVAFAGLGVMGFPMAGHLARAGHEVSVYNRSPDKARRWVEAHGGRMGETAAEAAAGAQVLALCVGNDDDVRQVVAQALP
ALADGAVIVDHTTTSAVVAREMAALAAQSGRAFVDAPVSGGQAGAEAGQLTIMCGGDEAAFAKVEPVIAAYAKAIRRMGG
PGAGQLTKMVNQICIAGVVQGLAEGLHFARRAGLDPQAVYEAISKGAAQSWQMDNRWKTMSEGRFDFGFAVDWMRKDLGL
LLDEATRNGATLEMTAMVDRFYAEVQALGGARWDTSSLVARLEKPE
>Mature_286_residues
MKVAFAGLGVMGFPMAGHLARAGHEVSVYNRSPDKARRWVEAHGGRMGETAAEAAAGAQVLALCVGNDDDVRQVVAQALP
ALADGAVIVDHTTTSAVVAREMAALAAQSGRAFVDAPVSGGQAGAEAGQLTIMCGGDEAAFAKVEPVIAAYAKAIRRMGG
PGAGQLTKMVNQICIAGVVQGLAEGLHFARRAGLDPQAVYEAISKGAAQSWQMDNRWKTMSEGRFDFGFAVDWMRKDLGL
LLDEATRNGATLEMTAMVDRFYAEVQALGGARWDTSSLVARLEKPE

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=27.719298245614, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=35.6643356643357, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI145693186, Length=291, Percent_Identity=34.3642611683849, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790315, Length=285, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=29.9651567944251, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=26.7657992565056, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=28.2312925170068, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=28.2312925170068, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=23.8434163701068, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30006; Mature: 30006

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAFAGLGVMGFPMAGHLARAGHEVSVYNRSPDKARRWVEAHGGRMGETAAEAAAGAQV
CEEEEECCCHHCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCEE
LALCVGNDDDVRQVVAQALPALADGAVIVDHTTTSAVVAREMAALAAQSGRAFVDAPVSG
EEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GQAGAEAGQLTIMCGGDEAAFAKVEPVIAAYAKAIRRMGGPGAGQLTKMVNQICIAGVVQ
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GLAEGLHFARRAGLDPQAVYEAISKGAAQSWQMDNRWKTMSEGRFDFGFAVDWMRKDLGL
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LLDEATRNGATLEMTAMVDRFYAEVQALGGARWDTSSLVARLEKPE
HHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKVAFAGLGVMGFPMAGHLARAGHEVSVYNRSPDKARRWVEAHGGRMGETAAEAAAGAQV
CEEEEECCCHHCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCEE
LALCVGNDDDVRQVVAQALPALADGAVIVDHTTTSAVVAREMAALAAQSGRAFVDAPVSG
EEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCC
GQAGAEAGQLTIMCGGDEAAFAKVEPVIAAYAKAIRRMGGPGAGQLTKMVNQICIAGVVQ
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GLAEGLHFARRAGLDPQAVYEAISKGAAQSWQMDNRWKTMSEGRFDFGFAVDWMRKDLGL
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LLDEATRNGATLEMTAMVDRFYAEVQALGGARWDTSSLVARLEKPE
HHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA