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The map label for this gene is tmk [H]

Identifier: 197105068

GI number: 197105068

Start: 1809935

End: 1810570

Strand: Reverse

Name: tmk [H]

Synonym: PHZ_c1605

Alternate gene names: 197105068

Gene position: 1810570-1809935 (Counterclockwise)

Preceding gene: 197105069

Following gene: 197105067

Centisome position: 45.31

GC content: 75.31

Gene sequence:

>636_bases
GTGGCGCGCGGGCGGTTCATCACATTCGAGGGCGGCGAGGGGGCCGGCAAGTCCACCCAGGTGAAGCGTCTGGCCGCGCG
CCTGGAGGCCCAGGGGCTGGAGGTCGTCACCACCCGCGAACCGGGCGGCTCCGCCGGCGCCGAGAGCATCCGCGCCCTGG
TGCTGAACGGCGAGGCCGACCGCTGGAGCCCGGTCACCGAGACCCTGCTGATGTACGCCGCCCGCCGCGACCACATCGAG
CGGGTGATCCGGCCGGCGCTCGCCCGCGGCGCCTGGGTGATCTGCGACCGCTTCGCCGACTCGACCCGCGCCTACCAGGG
CGCCGCCGGGGGCACGGACCCGGCGCTCATCGCGGCCCTCGAGGCCCATATCCTGGAGGACGTCCGGCCCGATCTGACGC
TCGTCTTCGACCTGCCGCCCGAGGTGGGGCTGGAGCGCGCCCACGCCCGCGCCGGCGCCGAGATGCGCTTCGAATCCAAG
GGCATGGCCTTCCACCAGCGCCTGCGCGAGGGCTTCCGGGCCATCGCCGCCGCCGAGCCGCAGCGCTGCGCCGTCATCGA
CGCCACGGCGCCGATGGATGCGGTGGAGGGCGCCGTCTGGACGGCCGTGTCCGAGCGGCTCGCCGTCCATGGCTGA

Upstream 100 bases:

>100_bases
GCCTACGCCCTGCGCGACCAGGTGGCCGCGTTCGGCTTCGCCGACGCCCGCGTCGTTCAGGCCTTCTAGACTTCTGGGCC
GAACGGGGCATTTTGGCCCC

Downstream 100 bases:

>100_bases
GGCCGCCGTCCCGCATCCCCGCGACGTCTACGAGCTGCGCGGGCAGGGGGGCGCCGAGGCCGCCTTCGAGGAGGCCCGCG
CCCGCTCGCGCCTGCACCAC

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MARGRFITFEGGEGAGKSTQVKRLAARLEAQGLEVVTTREPGGSAGAESIRALVLNGEADRWSPVTETLLMYAARRDHIE
RVIRPALARGAWVICDRFADSTRAYQGAAGGTDPALIAALEAHILEDVRPDLTLVFDLPPEVGLERAHARAGAEMRFESK
GMAFHQRLREGFRAIAAAEPQRCAVIDATAPMDAVEGAVWTAVSERLAVHG

Sequences:

>Translated_211_residues
MARGRFITFEGGEGAGKSTQVKRLAARLEAQGLEVVTTREPGGSAGAESIRALVLNGEADRWSPVTETLLMYAARRDHIE
RVIRPALARGAWVICDRFADSTRAYQGAAGGTDPALIAALEAHILEDVRPDLTLVFDLPPEVGLERAHARAGAEMRFESK
GMAFHQRLREGFRAIAAAEPQRCAVIDATAPMDAVEGAVWTAVSERLAVHG
>Mature_210_residues
ARGRFITFEGGEGAGKSTQVKRLAARLEAQGLEVVTTREPGGSAGAESIRALVLNGEADRWSPVTETLLMYAARRDHIER
VIRPALARGAWVICDRFADSTRAYQGAAGGTDPALIAALEAHILEDVRPDLTLVFDLPPEVGLERAHARAGAEMRFESKG
MAFHQRLREGFRAIAAAEPQRCAVIDATAPMDAVEGAVWTAVSERLAVHG

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=206, Percent_Identity=44.6601941747573, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 22684; Mature: 22553

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGRFITFEGGEGAGKSTQVKRLAARLEAQGLEVVTTREPGGSAGAESIRALVLNGEAD
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHEEEEECCCCC
RWSPVTETLLMYAARRDHIERVIRPALARGAWVICDRFADSTRAYQGAAGGTDPALIAAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
EAHILEDVRPDLTLVFDLPPEVGLERAHARAGAEMRFESKGMAFHQRLREGFRAIAAAEP
HHHHHHHCCCCEEEEEECCCHHCHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCC
QRCAVIDATAPMDAVEGAVWTAVSERLAVHG
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARGRFITFEGGEGAGKSTQVKRLAARLEAQGLEVVTTREPGGSAGAESIRALVLNGEAD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHEEEEECCCCC
RWSPVTETLLMYAARRDHIERVIRPALARGAWVICDRFADSTRAYQGAAGGTDPALIAAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHH
EAHILEDVRPDLTLVFDLPPEVGLERAHARAGAEMRFESKGMAFHQRLREGFRAIAAAEP
HHHHHHHCCCCEEEEEECCCHHCHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCC
QRCAVIDATAPMDAVEGAVWTAVSERLAVHG
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]