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The map label for this gene is rlmE [H]

Identifier: 197104878

GI number: 197104878

Start: 1600269

End: 1600988

Strand: Direct

Name: rlmE [H]

Synonym: PHZ_c1412

Alternate gene names: 197104878

Gene position: 1600269-1600988 (Clockwise)

Preceding gene: 197104877

Following gene: 197104880

Centisome position: 40.04

GC content: 70.0

Gene sequence:

>720_bases
ATGACCGAACCGCCCCGCAAGCGCATGGTGAAGCCGCCGACCGGCGGGCAGGAGGGCGGCCGCGGCAAGCCCGCGCGCCT
GAAGACCGCCAAGCAGCGCACCCCGTCACAGCAGGCCTGGCTCGAGCGCCAGGTGAACGATCCTTTCGCCGCCAAGGCGC
GAGCGCTGGGCTACCGCAGCCGGGCGGCCTTCAAGATCAGCGAGATCGACGACCGCTTCCACCTGTTCCGAAAGGGCGTG
CGGGTTGTCGACCTGGGTCTCGCCCCCGGCGGCTGGACCCAGGTGGCCATCCAGCGGGGCGTCACCGACATCGTGGGCGT
CGACCTGCTGCCGGTCGAGCCCCTGCCGCCGGCGCACATCCTGCAGATGGACTTCACCGACCCGGCCTGCGGTCCCAAGC
TGATCGAGCTGCTGGGCGGTCCCCCGGACGTGGTGATATCGGACATGGCGCCCAACACCATCGGCCATCGCGAGACCGAC
CACCTTCGCATCGTCGGCCTCATCGAGGCGGCGATCGACTTCGCGGTCGAAGTGCTGAAGCCGGGCGGGACGTTCGTGGC
CAAGGCCTTCCAGGGCGGCGGCACCGACCAGATCATCGCGGGGCTGAAGACCCGCTTCAAGGACGTCCGCAACATCAAGC
CGAAGGCCAGCCGGCAGGACAGCTCCGAGGTCTTCCTGGTCGCGACGGGGTTCAAGGGGCGGGCGGCTCCGGCGTCCTAG

Upstream 100 bases:

>100_bases
TGGCCGACCGCGGCCTGCGGGAAGGCATCCTCCTCTCCCTGATGTCCGAACGCGGGGGCCGCCGCCGCCGCAGGCGCCGC
CGCGGCGGCCGGAGCCCGTC

Downstream 100 bases:

>100_bases
CGCCTTCCGCCACCAGCCAGTCGCGGAACTTCGAAAGGGCCCGGCTTTCCGCCCCCGGCGTCGTCAGCATCGCGAAAGCG
CTGGCCGAGCGGTGGAAGCC

Product: cell division protein FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTEPPRKRMVKPPTGGQEGGRGKPARLKTAKQRTPSQQAWLERQVNDPFAAKARALGYRSRAAFKISEIDDRFHLFRKGV
RVVDLGLAPGGWTQVAIQRGVTDIVGVDLLPVEPLPPAHILQMDFTDPACGPKLIELLGGPPDVVISDMAPNTIGHRETD
HLRIVGLIEAAIDFAVEVLKPGGTFVAKAFQGGGTDQIIAGLKTRFKDVRNIKPKASRQDSSEVFLVATGFKGRAAPAS

Sequences:

>Translated_239_residues
MTEPPRKRMVKPPTGGQEGGRGKPARLKTAKQRTPSQQAWLERQVNDPFAAKARALGYRSRAAFKISEIDDRFHLFRKGV
RVVDLGLAPGGWTQVAIQRGVTDIVGVDLLPVEPLPPAHILQMDFTDPACGPKLIELLGGPPDVVISDMAPNTIGHRETD
HLRIVGLIEAAIDFAVEVLKPGGTFVAKAFQGGGTDQIIAGLKTRFKDVRNIKPKASRQDSSEVFLVATGFKGRAAPAS
>Mature_238_residues
TEPPRKRMVKPPTGGQEGGRGKPARLKTAKQRTPSQQAWLERQVNDPFAAKARALGYRSRAAFKISEIDDRFHLFRKGVR
VVDLGLAPGGWTQVAIQRGVTDIVGVDLLPVEPLPPAHILQMDFTDPACGPKLIELLGGPPDVVISDMAPNTIGHRETDH
LRIVGLIEAAIDFAVEVLKPGGTFVAKAFQGGGTDQIIAGLKTRFKDVRNIKPKASRQDSSEVFLVATGFKGRAAPAS

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=41.4746543778802, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=37.8947368421053, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=37.8947368421053, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=37.8947368421053, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=38.2978723404255, Blast_Score=124, Evalue=8e-29,
Organism=Escherichia coli, GI1789569, Length=207, Percent_Identity=42.0289855072464, Blast_Score=156, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17553474, Length=212, Percent_Identity=37.2641509433962, Blast_Score=143, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=35.8974358974359, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=37.3056994818653, Blast_Score=116, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71987561, Length=188, Percent_Identity=38.2978723404255, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=188, Percent_Identity=38.2978723404255, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=200, Percent_Identity=37.5, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=36.1256544502618, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=90, Percent_Identity=47.7777777777778, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356387, Length=239, Percent_Identity=37.2384937238494, Blast_Score=150, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=33.6734693877551, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=37.7659574468085, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647580, Length=199, Percent_Identity=33.6683417085427, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 25798; Mature: 25667

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPPRKRMVKPPTGGQEGGRGKPARLKTAKQRTPSQQAWLERQVNDPFAAKARALGYRS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCC
RAAFKISEIDDRFHLFRKGVRVVDLGLAPGGWTQVAIQRGVTDIVGVDLLPVEPLPPAHI
CCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHHCCCEECCCCCCCCEE
LQMDFTDPACGPKLIELLGGPPDVVISDMAPNTIGHRETDHLRIVGLIEAAIDFAVEVLK
EEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHC
PGGTFVAKAFQGGGTDQIIAGLKTRFKDVRNIKPKASRQDSSEVFLVATGFKGRAAPAS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TEPPRKRMVKPPTGGQEGGRGKPARLKTAKQRTPSQQAWLERQVNDPFAAKARALGYRS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCC
RAAFKISEIDDRFHLFRKGVRVVDLGLAPGGWTQVAIQRGVTDIVGVDLLPVEPLPPAHI
CCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHHCCCEECCCCCCCCEE
LQMDFTDPACGPKLIELLGGPPDVVISDMAPNTIGHRETDHLRIVGLIEAAIDFAVEVLK
EEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHC
PGGTFVAKAFQGGGTDQIIAGLKTRFKDVRNIKPKASRQDSSEVFLVATGFKGRAAPAS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA