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The map label for this gene is glnK [H]

Identifier: 197104827

GI number: 197104827

Start: 1543557

End: 1543895

Strand: Reverse

Name: glnK [H]

Synonym: PHZ_c1361

Alternate gene names: 197104827

Gene position: 1543895-1543557 (Counterclockwise)

Preceding gene: 197104833

Following gene: 197104826

Centisome position: 38.63

GC content: 66.96

Gene sequence:

>339_bases
ATGAAACTCATCATGGCGATCGTCAAACCCTTCAAGCTGGACGACGTGCGCGAAGCGCTCGTCGCCGCCGGCGTGGAGGG
GCTGACGGTCTCGGAAGTCAAAGGCTACGGCCGTCAGAAGGGGCAGACCGAGATCTATCGGGGCGCCGAGTACCAGATCA
ATTTCCTGCCGAAGGTGAAGCTCGAGGCCGTCGTCGACGACGCGGCGGCGGGCAAGGCCGTCGAGGCCATCAAGGCCGCG
GCCGCCACCGGCAAGATCGGCGACGGGAAGATCTTCGTCCTCGACGTCGCGGAGGCCGTGCGCATCCGGACGGGCGAGAC
CGGCGCCGCGGCGCTCTAA

Upstream 100 bases:

>100_bases
CGTCGGTCCGACCGCCGCCCAGGGGATGGGTCAAGTCAGCGGCGGTTTCGAACAACGAGAGGGGTGGGCCCGCCCTCAGG
GTCGGACGCGGGAGCGACGG

Downstream 100 bases:

>100_bases
GGGGACAAGGGGACAGACACTCATGAAATTGCTGGGATTCAATAGGCTGGCCGGGCTCGCGCTCGCCGCCCTGATCACGG
GAGCGCCCCTGGCCGCGCCC

Product: nitrogen regulatory protein P-II-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLIMAIVKPFKLDDVREALVAAGVEGLTVSEVKGYGRQKGQTEIYRGAEYQINFLPKVKLEAVVDDAAAGKAVEAIKAA
AATGKIGDGKIFVLDVAEAVRIRTGETGAAAL

Sequences:

>Translated_112_residues
MKLIMAIVKPFKLDDVREALVAAGVEGLTVSEVKGYGRQKGQTEIYRGAEYQINFLPKVKLEAVVDDAAAGKAVEAIKAA
AATGKIGDGKIFVLDVAEAVRIRTGETGAAAL
>Mature_112_residues
MKLIMAIVKPFKLDDVREALVAAGVEGLTVSEVKGYGRQKGQTEIYRGAEYQINFLPKVKLEAVVDDAAAGKAVEAIKAA
AATGKIGDGKIFVLDVAEAVRIRTGETGAAAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=65.1785714285714, Blast_Score=149, Evalue=6e-38,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=58.9285714285714, Blast_Score=142, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 11746; Mature: 11746

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIMAIVKPFKLDDVREALVAAGVEGLTVSEVKGYGRQKGQTEIYRGAEYQINFLPKVK
CCEEEEHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEECCCCEEEEECCCEE
LEAVVDDAAAGKAVEAIKAAAATGKIGDGKIFVLDVAEAVRIRTGETGAAAL
EHEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHEECCCCCCCCCC
>Mature Secondary Structure
MKLIMAIVKPFKLDDVREALVAAGVEGLTVSEVKGYGRQKGQTEIYRGAEYQINFLPKVK
CCEEEEHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEECCCCEEEEECCCEE
LEAVVDDAAAGKAVEAIKAAAATGKIGDGKIFVLDVAEAVRIRTGETGAAAL
EHEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA