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The map label for this gene is pyrB [H]

Identifier: 197104787

GI number: 197104787

Start: 1500360

End: 1501340

Strand: Reverse

Name: pyrB [H]

Synonym: PHZ_c1321

Alternate gene names: 197104787

Gene position: 1501340-1500360 (Counterclockwise)

Preceding gene: 197104788

Following gene: 197104786

Centisome position: 37.57

GC content: 68.09

Gene sequence:

>981_bases
ATGAGCGGCGCGCCCACCGGTCTGAACGAGGTCCTGCAGCGGACCTTTTCCTTCCCGAAGCGCCATTTCCTGTCCGCGAC
AGACCTCAACGAGGTTGAGGTGGCCAACCTCCTCGACCTCGCCGACGGCTTCGTCAGCCTGAACCGCCAGACCTCGAAGA
AGCTCGACCTGCTCAAGGGTCGCACGCTGATGAACCTGTTCTTCGAGAACTCGACCCGCACCCAGAGTTCGTTCGAGTTG
GCCGGCAAGCGGCTCGGCGCCGACGTGGTCAACATGAGCCCGCGCTCGTCGTCGATCTCCAAGGGCGAGACCCTGATCGA
CACGGCGGTGACGCTGAACGCCATGCAGCCGGACCTGCTGGTGATCCGGCATTCGTCCTCGGGCGCGGCGTCCCTGCTCT
CGCAGAAGGTCGGCTGTTCGGTGATCAACGCCGGCGACGGCCAGCACGAGCATCCCACGCAGGCTCTGCTCGACGCCCTG
TCCATGCGCCGCGCTTTCGGCCGCATCGCCGGCCTGAAGGTCGCCATCTGCGGCGACGTCCTGCACAGCCGCGTCGCCCG
CTCGAACATCGCCCTGCTGCAGATGCTGGGCGCCCACGTGCGGCTGGTCGGGCCGCCGACCCTGATCCCGGCCGCCGCCG
ACCGCTGGAACGTCGAGGTCTTCCACGACATGCGCAAGGGCATCGCCGGCTGCGACGTCGTGATGATGCTGCGCCTGCAG
CTCGAGCGCATGGACGGCGTCATGGCGCCGTCGCAGCGGGAATATTTCCGCTTCTTCGGCCTCGACCGCGAGAAGCTGGC
CCACGCCGCGCCGGGCGTGCGCGTCATGCACCCGGGTCCGATGAACCGCGGGGTGGAGATCGATTCCGACGTCGCCGACG
ACCTGGCGGTCAGCCTGATCCAGGACCAGGTCGAGATGGGCGTCGCCGCGCGCATGGCGGTCCTCACCTCGCTGGCGGCG
CGGCTGGACAACGAACGATGA

Upstream 100 bases:

>100_bases
AGATGGCCGCCGCCTGGATCCTCCAGGGCGCCCTGGACCGGCTGCGGAACCTCTAGAGCCCCTTGGCTTGGCGACCGGCG
CGGCCTATGACCGGCCCTCG

Downstream 100 bases:

>100_bases
GCGCCCGCCCCCTCGCCTTCGCCAACGTGCGCCTGCTGGACCCGGCGTCGGGGTTCGACGGTCCGGGCGCGGTGATCGTC
ACCGAGGGCGTGATCGCCGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSGAPTGLNEVLQRTFSFPKRHFLSATDLNEVEVANLLDLADGFVSLNRQTSKKLDLLKGRTLMNLFFENSTRTQSSFEL
AGKRLGADVVNMSPRSSSISKGETLIDTAVTLNAMQPDLLVIRHSSSGAASLLSQKVGCSVINAGDGQHEHPTQALLDAL
SMRRAFGRIAGLKVAICGDVLHSRVARSNIALLQMLGAHVRLVGPPTLIPAAADRWNVEVFHDMRKGIAGCDVVMMLRLQ
LERMDGVMAPSQREYFRFFGLDREKLAHAAPGVRVMHPGPMNRGVEIDSDVADDLAVSLIQDQVEMGVAARMAVLTSLAA
RLDNER

Sequences:

>Translated_326_residues
MSGAPTGLNEVLQRTFSFPKRHFLSATDLNEVEVANLLDLADGFVSLNRQTSKKLDLLKGRTLMNLFFENSTRTQSSFEL
AGKRLGADVVNMSPRSSSISKGETLIDTAVTLNAMQPDLLVIRHSSSGAASLLSQKVGCSVINAGDGQHEHPTQALLDAL
SMRRAFGRIAGLKVAICGDVLHSRVARSNIALLQMLGAHVRLVGPPTLIPAAADRWNVEVFHDMRKGIAGCDVVMMLRLQ
LERMDGVMAPSQREYFRFFGLDREKLAHAAPGVRVMHPGPMNRGVEIDSDVADDLAVSLIQDQVEMGVAARMAVLTSLAA
RLDNER
>Mature_325_residues
SGAPTGLNEVLQRTFSFPKRHFLSATDLNEVEVANLLDLADGFVSLNRQTSKKLDLLKGRTLMNLFFENSTRTQSSFELA
GKRLGADVVNMSPRSSSISKGETLIDTAVTLNAMQPDLLVIRHSSSGAASLLSQKVGCSVINAGDGQHEHPTQALLDALS
MRRAFGRIAGLKVAICGDVLHSRVARSNIALLQMLGAHVRLVGPPTLIPAAADRWNVEVFHDMRKGIAGCDVVMMLRLQL
ERMDGVMAPSQREYFRFFGLDREKLAHAAPGVRVMHPGPMNRGVEIDSDVADDLAVSLIQDQVEMGVAARMAVLTSLAAR
LDNER

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=34.6153846153846, Blast_Score=161, Evalue=8e-40,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=33.5483870967742, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790703, Length=233, Percent_Identity=28.755364806867, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786469, Length=231, Percent_Identity=27.7056277056277, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=31.6417910447761, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=325, Percent_Identity=34.4615384615385, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24642586, Length=335, Percent_Identity=33.4328358208955, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35423; Mature: 35292

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAPTGLNEVLQRTFSFPKRHFLSATDLNEVEVANLLDLADGFVSLNRQTSKKLDLLKG
CCCCCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
RTLMNLFFENSTRTQSSFELAGKRLGADVVNMSPRSSSISKGETLIDTAVTLNAMQPDLL
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEE
VIRHSSSGAASLLSQKVGCSVINAGDGQHEHPTQALLDALSMRRAFGRIAGLKVAICGDV
EEEECCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHSRVARSNIALLQMLGAHVRLVGPPTLIPAAADRWNVEVFHDMRKGIAGCDVVMMLRLQ
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
LERMDGVMAPSQREYFRFFGLDREKLAHAAPGVRVMHPGPMNRGVEIDSDVADDLAVSLI
HHHHCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
QDQVEMGVAARMAVLTSLAARLDNER
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGAPTGLNEVLQRTFSFPKRHFLSATDLNEVEVANLLDLADGFVSLNRQTSKKLDLLKG
CCCCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
RTLMNLFFENSTRTQSSFELAGKRLGADVVNMSPRSSSISKGETLIDTAVTLNAMQPDLL
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEE
VIRHSSSGAASLLSQKVGCSVINAGDGQHEHPTQALLDALSMRRAFGRIAGLKVAICGDV
EEEECCCCHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHSRVARSNIALLQMLGAHVRLVGPPTLIPAAADRWNVEVFHDMRKGIAGCDVVMMLRLQ
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
LERMDGVMAPSQREYFRFFGLDREKLAHAAPGVRVMHPGPMNRGVEIDSDVADDLAVSLI
HHHHCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHH
QDQVEMGVAARMAVLTSLAARLDNER
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA