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The map label for this gene is metG [H]

Identifier: 197104673

GI number: 197104673

Start: 1390500

End: 1392320

Strand: Direct

Name: metG [H]

Synonym: PHZ_c1207

Alternate gene names: 197104673

Gene position: 1390500-1392320 (Clockwise)

Preceding gene: 197104671

Following gene: 197104676

Centisome position: 34.8

GC content: 71.06

Gene sequence:

>1821_bases
ATGTCGCGCATCCTCATCACCTCGGCCCTGCCCTACATCAACGGGATCAAGCACCTGGGAACGCTCGCGGGCTCCATGCT
GCCGGCGGACGTCTATGCGCGCTTCCAGCGGGCGAGGGGGCGCGAGACCCTCTACATCTGCGCCACCGACGAGCACGGCA
CGCCGACCGAGCTGGCGGCCGCCGAGGCGGGCCTCGACCCGGCGACCTTCTGCGCCCAGCAGCACGAGGTGCAGCACCGG
CTGGGCCGCGCCTTCGGCCTCTCCTGGGACCACTTCGGCCGCTCGTCCTCGCCGCAGAACCACCGCCTGACCCAGCGTTT
CGCCCAGACCCTGTGGGAGGCGGGCTTCATCGAGGAGCGGGTCACCCAGCAGGTCTATTCCAACGCCGACAGGCGCTTCC
TGCCCGACCGCTACGTGATCGGGACCTGCCCGCACTGCGGCTACGAGGCCGCCCGCGGCGACCAGTGCGAGAACTGCACG
CGGGTGCTGGATCCGGCCGACCTGCTGCATCCGCGCTCGGCGGTCTCGGGCTCGACCGACATCGAGATCCGCGGCTCCAA
GCACCTGTTCCTGCGCCAGAGCCTGTTCGCCGGCAAGCTGCGCGACTGGATCGAGGCCAAGCGGGGCCGCTGGCCGCACC
TCGTCACCTCTATCGCCCTGAAGTGGCTGGACGAGGGGCTGCAGGACCGCGGCATCACCCGCGACCTGGAGTGGGGCGTG
CCGGTCAACGCCTTCGAATGGGGCCCGAACCCCGACGGCAAGCTGCCCGACGTCGAGGGGCTCGCCGGCAAGGTGTTCTA
CGTCTGGTTCGACGCGCCCATCGAGTACATCGGCGCCACCTGGGAATGGGCCGAGAGCCGCGCCGTCGAGGCAGGCCGCC
CCGGCGCGGACGACATCGAGTGGGAGCGCTGGTGGCGCGGCCCCGAGGCGTCGGACGTGACCTACGTGGAGTTCATGGGC
AAGGACAACGTGCCCTTCCACACCGTGGGCTTTCCCTGCACCCTGATCGGCGTGAACGAGCGCCTGGGCGCCGGCGGCGC
CTGGCGGGTGGCCGAGGCGCGGCCGTGGAAGCTGGTCGACGACCTCAAGGGCTTCAACTGGCTCGACTACTACGGCGGTA
AGTTCTCGACCTCGCAGGGCCGGGGCGTCTTCATGGACCACGCGCTGGAGCTGCTGCCGGCCGACTACTGGCGCTGGTGG
ATCATGGCCAACGCCCCCGAGACCTCGGACGCCACCTTCGCCTGGGAGCAGTTCCGCGACCAGGTGAACGCCGACCTCGC
CGACGTGCTCGGCAACTTCGTCAACCGCATCTGCAAGTTCGCCGAGAGCCGCTTCGAGGGCCAGGTCCCCGAGGGCGGCG
AGCCGGGCGAGCTGGAGACCGCGCTGGAAGCCGACGTGCTGATGAAGCTGCGCGAGCTGACCGGCCATCTCGAGGACCGC
GAGTTCCGCAAGGCCACCACGGCGCTGCGCCAGCTCTGGGTGCTGGGCAACCAGTACCTGACCGAGGCCGCGCCCTGGAC
CGCGATCAAGACCGACCGCGACCGCGCCGCGGTGGCGGTGCGCTACGGCCTGAACCTGGCGGCCCTGTTCGCCAAGGTCT
CCGAGCCCTTCATCCCGTTCGCCGCCGAGAAGGTGTCGCTGGCGCTGGGCGAGCCGTTCCCGGCGGCCTGGCCGGCGCAG
GACGGCGAGGGGCTGCTGACGGCGCTGGAGCCGGGACGCAGGATCGCCGCGCCCGAGGTGCTGTTCCGCAAGATCGAGGA
GACCCAGGTCGCCGAATGGCGCGAGCGCTTCGGCGGGCCGGAGGCGGCCGAGACCGCCTGA

Upstream 100 bases:

>100_bases
GCGCCGATAACCGCGTTTGGCGAGCTTTTTCGGCCGCGTGCTTTGGCGAAAGCCCCGCTCGGCCTATAAGCGCCGGTCTC
CGATCCAGGCCCCAAGATAC

Downstream 100 bases:

>100_bases
CGGAGCCTAGTGCGCCTCGCCGCCGCCGCCGTTCCTGTGGCGCAGGTGGGTGGCGACCTCCTCGGGCGTGATGTCCATGA
AGTCCGGGACCACGGCGTTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MSRILITSALPYINGIKHLGTLAGSMLPADVYARFQRARGRETLYICATDEHGTPTELAAAEAGLDPATFCAQQHEVQHR
LGRAFGLSWDHFGRSSSPQNHRLTQRFAQTLWEAGFIEERVTQQVYSNADRRFLPDRYVIGTCPHCGYEAARGDQCENCT
RVLDPADLLHPRSAVSGSTDIEIRGSKHLFLRQSLFAGKLRDWIEAKRGRWPHLVTSIALKWLDEGLQDRGITRDLEWGV
PVNAFEWGPNPDGKLPDVEGLAGKVFYVWFDAPIEYIGATWEWAESRAVEAGRPGADDIEWERWWRGPEASDVTYVEFMG
KDNVPFHTVGFPCTLIGVNERLGAGGAWRVAEARPWKLVDDLKGFNWLDYYGGKFSTSQGRGVFMDHALELLPADYWRWW
IMANAPETSDATFAWEQFRDQVNADLADVLGNFVNRICKFAESRFEGQVPEGGEPGELETALEADVLMKLRELTGHLEDR
EFRKATTALRQLWVLGNQYLTEAAPWTAIKTDRDRAAVAVRYGLNLAALFAKVSEPFIPFAAEKVSLALGEPFPAAWPAQ
DGEGLLTALEPGRRIAAPEVLFRKIEETQVAEWRERFGGPEAAETA

Sequences:

>Translated_606_residues
MSRILITSALPYINGIKHLGTLAGSMLPADVYARFQRARGRETLYICATDEHGTPTELAAAEAGLDPATFCAQQHEVQHR
LGRAFGLSWDHFGRSSSPQNHRLTQRFAQTLWEAGFIEERVTQQVYSNADRRFLPDRYVIGTCPHCGYEAARGDQCENCT
RVLDPADLLHPRSAVSGSTDIEIRGSKHLFLRQSLFAGKLRDWIEAKRGRWPHLVTSIALKWLDEGLQDRGITRDLEWGV
PVNAFEWGPNPDGKLPDVEGLAGKVFYVWFDAPIEYIGATWEWAESRAVEAGRPGADDIEWERWWRGPEASDVTYVEFMG
KDNVPFHTVGFPCTLIGVNERLGAGGAWRVAEARPWKLVDDLKGFNWLDYYGGKFSTSQGRGVFMDHALELLPADYWRWW
IMANAPETSDATFAWEQFRDQVNADLADVLGNFVNRICKFAESRFEGQVPEGGEPGELETALEADVLMKLRELTGHLEDR
EFRKATTALRQLWVLGNQYLTEAAPWTAIKTDRDRAAVAVRYGLNLAALFAKVSEPFIPFAAEKVSLALGEPFPAAWPAQ
DGEGLLTALEPGRRIAAPEVLFRKIEETQVAEWRERFGGPEAAETA
>Mature_605_residues
SRILITSALPYINGIKHLGTLAGSMLPADVYARFQRARGRETLYICATDEHGTPTELAAAEAGLDPATFCAQQHEVQHRL
GRAFGLSWDHFGRSSSPQNHRLTQRFAQTLWEAGFIEERVTQQVYSNADRRFLPDRYVIGTCPHCGYEAARGDQCENCTR
VLDPADLLHPRSAVSGSTDIEIRGSKHLFLRQSLFAGKLRDWIEAKRGRWPHLVTSIALKWLDEGLQDRGITRDLEWGVP
VNAFEWGPNPDGKLPDVEGLAGKVFYVWFDAPIEYIGATWEWAESRAVEAGRPGADDIEWERWWRGPEASDVTYVEFMGK
DNVPFHTVGFPCTLIGVNERLGAGGAWRVAEARPWKLVDDLKGFNWLDYYGGKFSTSQGRGVFMDHALELLPADYWRWWI
MANAPETSDATFAWEQFRDQVNADLADVLGNFVNRICKFAESRFEGQVPEGGEPGELETALEADVLMKLRELTGHLEDRE
FRKATTALRQLWVLGNQYLTEAAPWTAIKTDRDRAAVAVRYGLNLAALFAKVSEPFIPFAAEKVSLALGEPFPAAWPAQD
GEGLLTALEPGRRIAAPEVLFRKIEETQVAEWRERFGGPEAAETA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=605, Percent_Identity=36.6942148760331, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI24308436, Length=572, Percent_Identity=24.4755244755245, Blast_Score=126, Evalue=7e-29,
Organism=Escherichia coli, GI1788432, Length=594, Percent_Identity=30.8080808080808, Blast_Score=254, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17540794, Length=625, Percent_Identity=32.8, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI212645096, Length=566, Percent_Identity=24.0282685512367, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321704, Length=606, Percent_Identity=34.1584158415842, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655311, Length=616, Percent_Identity=36.038961038961, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI21358483, Length=567, Percent_Identity=23.1040564373898, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 68026; Mature: 67895

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILITSALPYINGIKHLGTLAGSMLPADVYARFQRARGRETLYICATDEHGTPTELAA
CCCEEEEHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
AEAGLDPATFCAQQHEVQHRLGRAFGLSWDHFGRSSSPQNHRLTQRFAQTLWEAGFIEER
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
VTQQVYSNADRRFLPDRYVIGTCPHCGYEAARGDQCENCTRVLDPADLLHPRSAVSGSTD
HHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCHHHCCCHHHCCCCCC
IEIRGSKHLFLRQSLFAGKLRDWIEAKRGRWPHLVTSIALKWLDEGLQDRGITRDLEWGV
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PVNAFEWGPNPDGKLPDVEGLAGKVFYVWFDAPIEYIGATWEWAESRAVEAGRPGADDIE
CCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCCCCCCCCC
WERWWRGPEASDVTYVEFMGKDNVPFHTVGFPCTLIGVNERLGAGGAWRVAEARPWKLVD
HHHHCCCCCCCCEEEEEEECCCCCCEEECCCCEEEEECCCCCCCCCCEEECCCCCCHHHH
DLKGFNWLDYYGGKFSTSQGRGVFMDHALELLPADYWRWWIMANAPETSDATFAWEQFRD
HCCCCCCHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
QVNADLADVLGNFVNRICKFAESRFEGQVPEGGEPGELETALEADVLMKLRELTGHLEDR
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
EFRKATTALRQLWVLGNQYLTEAAPWTAIKTDRDRAAVAVRYGLNLAALFAKVSEPFIPF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCH
AAEKVSLALGEPFPAAWPAQDGEGLLTALEPGRRIAAPEVLFRKIEETQVAEWRERFGGP
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EAAETA
CCCCCC
>Mature Secondary Structure 
SRILITSALPYINGIKHLGTLAGSMLPADVYARFQRARGRETLYICATDEHGTPTELAA
CCEEEEHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
AEAGLDPATFCAQQHEVQHRLGRAFGLSWDHFGRSSSPQNHRLTQRFAQTLWEAGFIEER
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
VTQQVYSNADRRFLPDRYVIGTCPHCGYEAARGDQCENCTRVLDPADLLHPRSAVSGSTD
HHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCHHHCCCHHHCCCCCC
IEIRGSKHLFLRQSLFAGKLRDWIEAKRGRWPHLVTSIALKWLDEGLQDRGITRDLEWGV
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PVNAFEWGPNPDGKLPDVEGLAGKVFYVWFDAPIEYIGATWEWAESRAVEAGRPGADDIE
CCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCCCCCCCCC
WERWWRGPEASDVTYVEFMGKDNVPFHTVGFPCTLIGVNERLGAGGAWRVAEARPWKLVD
HHHHCCCCCCCCEEEEEEECCCCCCEEECCCCEEEEECCCCCCCCCCEEECCCCCCHHHH
DLKGFNWLDYYGGKFSTSQGRGVFMDHALELLPADYWRWWIMANAPETSDATFAWEQFRD
HCCCCCCHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
QVNADLADVLGNFVNRICKFAESRFEGQVPEGGEPGELETALEADVLMKLRELTGHLEDR
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
EFRKATTALRQLWVLGNQYLTEAAPWTAIKTDRDRAAVAVRYGLNLAALFAKVSEPFIPF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCH
AAEKVSLALGEPFPAAWPAQDGEGLLTALEPGRRIAAPEVLFRKIEETQVAEWRERFGGP
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
EAAETA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA