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The map label for this gene is tufA [H]

Identifier: 197104676

GI number: 197104676

Start: 1394298

End: 1395488

Strand: Direct

Name: tufA [H]

Synonym: PHZ_c1210

Alternate gene names: 197104676

Gene position: 1394298-1395488 (Clockwise)

Preceding gene: 197104673

Following gene: 197104677

Centisome position: 34.89

GC content: 65.58

Gene sequence:

>1191_bases
ATGGCCAAAGAGAAGTTTGAACGCACGAAGCCGCACTGCAACATCGGCACGATTGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCGGCGATCACGATCACGCTGGCGAAGTCTGGTGGTGCGACGGCGAAGAACTACGCCGACATCGACGCGGCGC
CGGAAGAGAAGGCGCGCGGGATCACGATCAACACGGCGCACGTGGAATACGAGACGGCGAACCGTCACTACGCGCACGTG
GACTGCCCTGGCCACGCCGACTACGTGAAGAACATGATCACGGGCGCGGCGCAGATGGACGGCGCGATCCTGGTGGTGAG
CGCTGCGGACGGTCCGATGCCGCAGACCCGCGAGCACATCCTGCTGGCGCGTCAGGTGGGCGTGCCGGCGCTGGTGGTGT
TCATGAACAAGGTCGACATGGTCGACGACGCCGAGCTGCTGGACCTGGTGGAGATGGAAGTGCGCGAGCTGCTTTCGTCG
TACCAGTTCCCGGGCGACGACATCCCGATCGTGAAGGGCTCGGCCCTGGCGGCGGTCGAGGGCCGTGACCCGCAGATCGG
CGAGGAGCGGATCCTGGAGCTGATGGCGGCGGTGGACAGCTACATCCCGCAGCCGGAGCGTCCGATCGACCAGCCGTTCC
TGATGCCGGTGGAAGACGTCTTTTCGATCTCGGGCCGCGGCACGGTTGTGACGGGCCGGATCGAGAAGGGCATCGTGAAG
GTCGGCGACGAAGTGGAGATCGTCGGCATCCGCGAAGTGCAGAAGACGACCTGCACGGGCGTGGAGATGTTCCGCAAGCT
GCTGGACCAGGGCCAGGCGGGCGACAACGTGGGCGTGCTGCTGCGCGGCACGAAGCGCGAGGACGTGGAGCGCGGCCAGG
TGCTGTGCAAGCCGGGCTCGATCACCCCGCACACCGAGTTCATGGCCGAGGCCTACATCCTGACGAAGGAAGAGGGCGGC
CGTCACACCCCGTTCTTCACGAACTACCGTCCGCAGTTCTACTTCCGGACGACGGACGTGACGGGGATCATCAAGCTGAA
GGAAGGCGTGGAGATGATCATGCCCGGCGACAACGCCGAGCTGCTCGTCGAGCTGATCACGCCGATCGCCATGGACCAGG
GCCTGCGCTTCGCGATCCGCGAAGGCGGCCGGACCGTCGGCGCCGGCGTCGTGGCCAAGATCATCAAGTAA

Upstream 100 bases:

>100_bases
GTAGTGCAGCAGCCTTCCAAGCTGAGGATGCGGGTTCGATTCCCGCTACCCGCTCCAGCTCCTTGATTAGACAGTATCAA
CAAACGCCGGCAGGACCCTG

Downstream 100 bases:

>100_bases
GGCCGCTAAAGCCCGTCATCCCCGGCCCACGGAGCGGCGAAGCCGCGAAGGCCGAGCCGGGGAGCCAACTCGAAGAGGCC
GCCGGGTCACCCCGGCGGCC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHCNIGTIGHVDHGKTTLTAAITITLAKSGGATAKNYADIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDAELLDLVEMEVRELLSS
YQFPGDDIPIVKGSALAAVEGRDPQIGEERILELMAAVDSYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRIEKGIVK
VGDEVEIVGIREVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTEFMAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGIIKLKEGVEMIMPGDNAELLVELITPIAMDQGLRFAIREGGRTVGAGVVAKIIK

Sequences:

>Translated_396_residues
MAKEKFERTKPHCNIGTIGHVDHGKTTLTAAITITLAKSGGATAKNYADIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDAELLDLVEMEVRELLSS
YQFPGDDIPIVKGSALAAVEGRDPQIGEERILELMAAVDSYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRIEKGIVK
VGDEVEIVGIREVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTEFMAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGIIKLKEGVEMIMPGDNAELLVELITPIAMDQGLRFAIREGGRTVGAGVVAKIIK
>Mature_395_residues
AKEKFERTKPHCNIGTIGHVDHGKTTLTAAITITLAKSGGATAKNYADIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDAELLDLVEMEVRELLSSY
QFPGDDIPIVKGSALAAVEGRDPQIGEERILELMAAVDSYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRIEKGIVKV
GDEVEIVGIREVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTEFMAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGIIKLKEGVEMIMPGDNAELLVELITPIAMDQGLRFAIREGGRTVGAGVVAKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.8527918781726, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=31.6027088036117, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=31.6027088036117, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=29.1390728476821, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4503471, Length=453, Percent_Identity=28.9183222958057, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=29.874213836478, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI46094014, Length=325, Percent_Identity=28.9230769230769, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194018522, Length=334, Percent_Identity=28.4431137724551, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI194018520, Length=334, Percent_Identity=28.4431137724551, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI194097354, Length=334, Percent_Identity=28.4431137724551, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI4503507, Length=270, Percent_Identity=29.2592592592593, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=34.3511450381679, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI157426893, Length=291, Percent_Identity=25.4295532646048, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=77.7215189873418, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=77.7215189873418, Blast_Score=612, Evalue=1e-177,
Organism=Escherichia coli, GI2367247, Length=380, Percent_Identity=32.1052631578947, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=34.5833333333333, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789108, Length=317, Percent_Identity=28.7066246056782, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=32.1917808219178, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=53.7878787878788, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.9276485788114, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=455, Percent_Identity=30.5494505494506, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=455, Percent_Identity=30.5494505494506, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=28.604118993135, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=29.9086757990868, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=28.604118993135, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=25.8849557522124, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=29.6666666666667, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=173, Percent_Identity=36.4161849710983, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=275, Percent_Identity=28, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=253, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.6565656565657, Blast_Score=541, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=28.7640449438202, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=28.7640449438202, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=451, Percent_Identity=23.9467849223947, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=27.8260869565217, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=28.3154121863799, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=177, Percent_Identity=29.9435028248588, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.2875318066158, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.2875318066158, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=49.4818652849741, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=29.3859649122807, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=29.3859649122807, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=29.3859649122807, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=29.3859649122807, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=29.1028446389497, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=29.1028446389497, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.3630363036304, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550900, Length=445, Percent_Identity=27.6404494382022, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17137380, Length=326, Percent_Identity=30.6748466257669, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.2527881040892, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.6245353159851, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28572034, Length=167, Percent_Identity=34.7305389221557, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43149; Mature: 43018

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHCNIGTIGHVDHGKTTLTAAITITLAKSGGATAKNYADIDAAPEEKARG
CCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFMNKVDMVDDAELLDLVEMEVRELLSSYQFPGDDIPIVKGSALAAVE
HHHHHCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
GRDPQIGEERILELMAAVDSYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRIEKGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCEE
VGDEVEIVGIREVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGS
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
ITPHTEFMAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIIKLKEGVEMIMPGDNAE
CCCCHHHHHEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEEECCCCEEEECCCCHH
LLVELITPIAMDQGLRFAIREGGRTVGAGVVAKIIK
HHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHCNIGTIGHVDHGKTTLTAAITITLAKSGGATAKNYADIDAAPEEKARG
CCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFMNKVDMVDDAELLDLVEMEVRELLSSYQFPGDDIPIVKGSALAAVE
HHHHHCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
GRDPQIGEERILELMAAVDSYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRIEKGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCEE
VGDEVEIVGIREVQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGS
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
ITPHTEFMAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIIKLKEGVEMIMPGDNAE
CCCCHHHHHEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEEECCCCEEEECCCCHH
LLVELITPIAMDQGLRFAIREGGRTVGAGVVAKIIK
HHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA