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The map label for this gene is fadB [H]

Identifier: 197104574

GI number: 197104574

Start: 1262851

End: 1263633

Strand: Direct

Name: fadB [H]

Synonym: PHZ_c1108

Alternate gene names: 197104574

Gene position: 1262851-1263633 (Clockwise)

Preceding gene: 197104573

Following gene: 197104575

Centisome position: 31.6

GC content: 71.14

Gene sequence:

>783_bases
ATGGCGTACGAGTTCATCCGGGTGGAGCGGGAAGGGCCGATCACCACCTTCATCCTGAACCGGCCCGAGGTGATGAACGC
GCTGCATTCGCCGGCGCACTTCGAGCTGCACGAGGCCTTCGACGCCTTCGCCGCCGACCCGGACCAGTGGGTGGGCATCG
TCACAGGGGCGGGCGAGAAGGCCTTTTCGGCCGGCAACGACCTGAAGCACCAGGCCTCCGGCGGGGCGATGAAGAGCCCG
CCCAGCGGGTTCGCCGGCCTGACCTCGCGGTTCGACCTGAACAAGCCGCTGATCGCGGCGGTGAACGGCGTGGCCATGGG
CGGCGGGTTCGAGATCGCCCTGGCCTGCGACATCATCATCGCCTCCGAGGCGGCGACCTTCGCCCTGCCCGAGCCGCGGG
TGGGCCTGGCGGCCCTGGCCGGCGGCCTGCACCGGTTGCCCCGGGCCATCGGCGTCAAACGGGCCATGGCTCTGATCCTC
ACCGCCCGGCGCGTGTCGGCGGCCGAGGGCAAGGAGTTGGGCTTCGTGGCCGAGGTCACCGCGCCGCAGGACCTGATGCG
CACGGCGCGCGCGTGGGCCCAGCAGGTCTGCGAGCTTTCGCCGATGTCGATCCGCGCCTCGAAGGAGGCCGTCTACAAGG
GCCTGGACGAGCCGACGCTGGAGGCGGCGATCCGCGGCCAGGGCAAGTATCCGGCCGTGGCGGCGCTGTTCACCTCGGAG
GACTTCAAGGAAGGGCCGATGGCCTTCGCCCAGAAGCGCCCGCCGCAGTGGAAGGGCCGCTGA

Upstream 100 bases:

>100_bases
GTCGCCGCCCTCCAGGGCGCCTGCGTGGCCGAACCCGCGCCTTGACGGACGTGGCGGCGCCCCTCCCTTGATGCCCGCAA
GGCACAAGGGAGGACGGGCC

Downstream 100 bases:

>100_bases
CGGCCTGCTGAGGGACCCTTGTTCCCCGGCGTCAATCCCGTCACATTCCGCCCCGAGGACGGGGGTTGCGTATGGTGGAT
GTGGACGCGGCGGGCGCAGT

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAYEFIRVEREGPITTFILNRPEVMNALHSPAHFELHEAFDAFAADPDQWVGIVTGAGEKAFSAGNDLKHQASGGAMKSP
PSGFAGLTSRFDLNKPLIAAVNGVAMGGGFEIALACDIIIASEAATFALPEPRVGLAALAGGLHRLPRAIGVKRAMALIL
TARRVSAAEGKELGFVAEVTAPQDLMRTARAWAQQVCELSPMSIRASKEAVYKGLDEPTLEAAIRGQGKYPAVAALFTSE
DFKEGPMAFAQKRPPQWKGR

Sequences:

>Translated_260_residues
MAYEFIRVEREGPITTFILNRPEVMNALHSPAHFELHEAFDAFAADPDQWVGIVTGAGEKAFSAGNDLKHQASGGAMKSP
PSGFAGLTSRFDLNKPLIAAVNGVAMGGGFEIALACDIIIASEAATFALPEPRVGLAALAGGLHRLPRAIGVKRAMALIL
TARRVSAAEGKELGFVAEVTAPQDLMRTARAWAQQVCELSPMSIRASKEAVYKGLDEPTLEAAIRGQGKYPAVAALFTSE
DFKEGPMAFAQKRPPQWKGR
>Mature_259_residues
AYEFIRVEREGPITTFILNRPEVMNALHSPAHFELHEAFDAFAADPDQWVGIVTGAGEKAFSAGNDLKHQASGGAMKSPP
SGFAGLTSRFDLNKPLIAAVNGVAMGGGFEIALACDIIIASEAATFALPEPRVGLAALAGGLHRLPRAIGVKRAMALILT
ARRVSAAEGKELGFVAEVTAPQDLMRTARAWAQQVCELSPMSIRASKEAVYKGLDEPTLEAAIRGQGKYPAVAALFTSED
FKEGPMAFAQKRPPQWKGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=262, Percent_Identity=36.2595419847328, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI70995211, Length=238, Percent_Identity=31.5126050420168, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=31.6205533596838, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=27.9816513761468, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=27.9816513761468, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=27.9816513761468, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI37594469, Length=173, Percent_Identity=32.3699421965318, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594471, Length=173, Percent_Identity=32.3699421965318, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=28.8888888888889, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=27.8884462151394, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=28.5087719298246, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=28.5087719298246, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI68989263, Length=225, Percent_Identity=31.5555555555556, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=28.968253968254, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694520, Length=187, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=45.4545454545455, Blast_Score=196, Evalue=9e-52,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=35.2941176470588, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=30.4182509505703, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=27.4436090225564, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=32.9545454545455, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788682, Length=151, Percent_Identity=31.7880794701987, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=29.3814432989691, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=37.7862595419847, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=34.2412451361868, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=224, Percent_Identity=29.0178571428571, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=32.6829268292683, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=269, Percent_Identity=27.5092936802974, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=30.0884955752212, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=224, Percent_Identity=27.2321428571429, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=226, Percent_Identity=26.5486725663717, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=28.0952380952381, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=30.6010928961749, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=30.6010928961749, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=183, Percent_Identity=30.6010928961749, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=260, Percent_Identity=27.3076923076923, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=196, Percent_Identity=25.5102040816327, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=34.7490347490347, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=34.7490347490347, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=31.2741312741313, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=29.3893129770992, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=30.9090909090909, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24650670, Length=181, Percent_Identity=33.1491712707182, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=24.609375, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=24.7104247104247, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571730, Length=159, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28571729, Length=159, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27700; Mature: 27569

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYEFIRVEREGPITTFILNRPEVMNALHSPAHFELHEAFDAFAADPDQWVGIVTGAGEK
CCCEEEEEECCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCH
AFSAGNDLKHQASGGAMKSPPSGFAGLTSRFDLNKPLIAAVNGVAMGGGFEIALACDIII
HHCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHCCEEECCCEEEEEEEEEEE
ASEAATFALPEPRVGLAALAGGLHRLPRAIGVKRAMALILTARRVSAAEGKELGFVAEVT
ECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
APQDLMRTARAWAQQVCELSPMSIRASKEAVYKGLDEPTLEAAIRGQGKYPAVAALFTSE
CCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCC
DFKEGPMAFAQKRPPQWKGR
CCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYEFIRVEREGPITTFILNRPEVMNALHSPAHFELHEAFDAFAADPDQWVGIVTGAGEK
CCEEEEEECCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCH
AFSAGNDLKHQASGGAMKSPPSGFAGLTSRFDLNKPLIAAVNGVAMGGGFEIALACDIII
HHCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHCCEEECCCEEEEEEEEEEE
ASEAATFALPEPRVGLAALAGGLHRLPRAIGVKRAMALILTARRVSAAEGKELGFVAEVT
ECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
APQDLMRTARAWAQQVCELSPMSIRASKEAVYKGLDEPTLEAAIRGQGKYPAVAALFTSE
CCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCC
DFKEGPMAFAQKRPPQWKGR
CCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA