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The map label for this gene is mdtE [H]

Identifier: 197104543

GI number: 197104543

Start: 1215795

End: 1217006

Strand: Direct

Name: mdtE [H]

Synonym: PHZ_c1077

Alternate gene names: 197104543

Gene position: 1215795-1217006 (Clockwise)

Preceding gene: 197104542

Following gene: 197104544

Centisome position: 30.42

GC content: 74.5

Gene sequence:

>1212_bases
ATGTCGGGGACGGTCCGTTTGGCCGTTGCGGCCGCGTTGTCGCTCGTGCTGAGCGCATGCGGGGCGAGGAAGGCGGAGCC
CGTGCAGGGTCCGCCGACGGTGGCCGTGTCGGCGCCGCTGCAGCGCGAGATCGTCGACTGGGACGAGTTCGTCGGCCGCT
TCGAGGCGGTGGACGAGGTCGAGGTGCGCCCGCGCGTCTCGGGCTACGTCCGCTCGATCCATTTCCGCGACGGCGACCTG
GTCCGGAAGGGCCAGCTGCTGTTCGTCATCGACCCCCGGCCGTACGAGGCGGCCCTGGCCCAGGCCCGCGCCGAGGTGCG
GCAGGCTGAGGCCCAGGCGGCCCGGGCCAAGCTGGAGCTGGAGCGGGCGCAGAAGTTGCTGGAGACGGGCTTCGTCAGCC
GGGCGGTCCACGACGCGCGGCTGGCCGAGGCGCGGTCCGCCGAGGCGGCGGTGGGCGCGGCGCGCGCCGCCGAGCAGGCG
CGGGCGCTGGACGTCTCGTTCACCCGCGTGACCTCGCCGATCTCGGGCCGGGTGTCGGACCGGCGGGTGAGCGTGGGCAA
CCTGGTCGCCGCCGAGGGCGCCGAGCCGACCCTGCTGACCACGGTGGTCTCGCTGGACCCGATCCGCTTCGCCTTCACGG
GCTCGGAGAGCGTCTACCTGAAGTACCAGCGGCAGAACCGCGACGGCTCGCGGCCCAGCTCGCGCAGCGCGGCCAATCCG
GTGGAGATCCAGCTGCAGGACGAGACCGGCTACGCCTGGCGCGGGCGGATGGACTTCGTGGACAACGCCCTCGACACCGG
CTCGGGCACGATCCGCGGCCGGGCGATCGTGCCCAACCCCGACCTGTTCCTGACCCCGGGCATGTACGGCCGGATGCGGC
TGCTGGGCTCGGGCGCCTATCCCGCGCTGCTGGTCCCGGACGCGGCGGTGATGACCGACCAGTCGGACAAGATCGTGCTG
GTGGTGGCGAGCGGGAACGTCGTCCAGCCCCGCAAGGTGGAGCTGGGCCCGCTGGTCGACGGCCTGCGGGTCGTGCGCGC
CGGGCTGGAGGCGGGCGACCGGGTGGTCGTCGAGGGCCAGCAGCGGGTGCGTCCGGGAATGAAGGTCGTGCCGCAGCCGG
GCCGGATCGTGGCCCAGGCGCCGACCGCGCCGCGCCCGGATGTCGTCTCTCCCCCGGCCGCCCAGGCCACCGCCGCCGCC
AACGCGGAGTAG

Upstream 100 bases:

>100_bases
CTCTGGACAGGGCCTGGGAGCGTTCCCAGCTAGGACGACGAGCGCCGCCGCGACGGGCGTCCGACGCTCAGCCAGATCCA
CATTCCAGGGGGAATTCCGC

Downstream 100 bases:

>100_bases
GCCCGTGCGTTTCTCCAGGTTCTTCATCGACCGGCCGATCTTCGCGGCCGTGATCTCGATCATCATCGTGCTGGTGGGGG
CCTTCGCCTATCCCACCCTG

Product: multidrug-efflux system secretion protein, HlyD family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSGTVRLAVAAALSLVLSACGARKAEPVQGPPTVAVSAPLQREIVDWDEFVGRFEAVDEVEVRPRVSGYVRSIHFRDGDL
VRKGQLLFVIDPRPYEAALAQARAEVRQAEAQAARAKLELERAQKLLETGFVSRAVHDARLAEARSAEAAVGAARAAEQA
RALDVSFTRVTSPISGRVSDRRVSVGNLVAAEGAEPTLLTTVVSLDPIRFAFTGSESVYLKYQRQNRDGSRPSSRSAANP
VEIQLQDETGYAWRGRMDFVDNALDTGSGTIRGRAIVPNPDLFLTPGMYGRMRLLGSGAYPALLVPDAAVMTDQSDKIVL
VVASGNVVQPRKVELGPLVDGLRVVRAGLEAGDRVVVEGQQRVRPGMKVVPQPGRIVAQAPTAPRPDVVSPPAAQATAAA
NAE

Sequences:

>Translated_403_residues
MSGTVRLAVAAALSLVLSACGARKAEPVQGPPTVAVSAPLQREIVDWDEFVGRFEAVDEVEVRPRVSGYVRSIHFRDGDL
VRKGQLLFVIDPRPYEAALAQARAEVRQAEAQAARAKLELERAQKLLETGFVSRAVHDARLAEARSAEAAVGAARAAEQA
RALDVSFTRVTSPISGRVSDRRVSVGNLVAAEGAEPTLLTTVVSLDPIRFAFTGSESVYLKYQRQNRDGSRPSSRSAANP
VEIQLQDETGYAWRGRMDFVDNALDTGSGTIRGRAIVPNPDLFLTPGMYGRMRLLGSGAYPALLVPDAAVMTDQSDKIVL
VVASGNVVQPRKVELGPLVDGLRVVRAGLEAGDRVVVEGQQRVRPGMKVVPQPGRIVAQAPTAPRPDVVSPPAAQATAAA
NAE
>Mature_402_residues
SGTVRLAVAAALSLVLSACGARKAEPVQGPPTVAVSAPLQREIVDWDEFVGRFEAVDEVEVRPRVSGYVRSIHFRDGDLV
RKGQLLFVIDPRPYEAALAQARAEVRQAEAQAARAKLELERAQKLLETGFVSRAVHDARLAEARSAEAAVGAARAAEQAR
ALDVSFTRVTSPISGRVSDRRVSVGNLVAAEGAEPTLLTTVVSLDPIRFAFTGSESVYLKYQRQNRDGSRPSSRSAANPV
EIQLQDETGYAWRGRMDFVDNALDTGSGTIRGRAIVPNPDLFLTPGMYGRMRLLGSGAYPALLVPDAAVMTDQSDKIVLV
VASGNVVQPRKVELGPLVDGLRVVRAGLEAGDRVVVEGQQRVRPGMKVVPQPGRIVAQAPTAPRPDVVSPPAAQATAAAN
AE

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=355, Percent_Identity=33.8028169014084, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1786668, Length=379, Percent_Identity=37.467018469657, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1789665, Length=370, Percent_Identity=34.5945945945946, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87082036, Length=342, Percent_Identity=31.2865497076023, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI87081796, Length=327, Percent_Identity=26.605504587156, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790519, Length=262, Percent_Identity=28.2442748091603, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081951, Length=163, Percent_Identity=26.9938650306748, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42990; Mature: 42859

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTVRLAVAAALSLVLSACGARKAEPVQGPPTVAVSAPLQREIVDWDEFVGRFEAVDEV
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHCCHHHHHHHHHCCCCC
EVRPRVSGYVRSIHFRDGDLVRKGQLLFVIDPRPYEAALAQARAEVRQAEAQAARAKLEL
CCCCCCCCEEEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAQKLLETGFVSRAVHDARLAEARSAEAAVGAARAAEQARALDVSFTRVTSPISGRVSD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCCC
RRVSVGNLVAAEGAEPTLLTTVVSLDPIRFAFTGSESVYLKYQRQNRDGSRPSSRSAANP
CEEECCCEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCC
VEIQLQDETGYAWRGRMDFVDNALDTGSGTIRGRAIVPNPDLFLTPGMYGRMRLLGSGAY
EEEEEECCCCCEEECCHHHHHHHHCCCCCCEECEEECCCCCEEECCCCCCEEEEEECCCC
PALLVPDAAVMTDQSDKIVLVVASGNVVQPRKVELGPLVDGLRVVRAGLEAGDRVVVEGQ
CEEECCCCCEEECCCCCEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEECH
QRVRPGMKVVPQPGRIVAQAPTAPRPDVVSPPAAQATAAANAE
HHCCCCCEECCCCCCEEEECCCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SGTVRLAVAAALSLVLSACGARKAEPVQGPPTVAVSAPLQREIVDWDEFVGRFEAVDEV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHCCHHHHHHHHHCCCCC
EVRPRVSGYVRSIHFRDGDLVRKGQLLFVIDPRPYEAALAQARAEVRQAEAQAARAKLEL
CCCCCCCCEEEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAQKLLETGFVSRAVHDARLAEARSAEAAVGAARAAEQARALDVSFTRVTSPISGRVSD
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCCCC
RRVSVGNLVAAEGAEPTLLTTVVSLDPIRFAFTGSESVYLKYQRQNRDGSRPSSRSAANP
CEEECCCEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCC
VEIQLQDETGYAWRGRMDFVDNALDTGSGTIRGRAIVPNPDLFLTPGMYGRMRLLGSGAY
EEEEEECCCCCEEECCHHHHHHHHCCCCCCEECEEECCCCCEEECCCCCCEEEEEECCCC
PALLVPDAAVMTDQSDKIVLVVASGNVVQPRKVELGPLVDGLRVVRAGLEAGDRVVVEGQ
CEEECCCCCEEECCCCCEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEECH
QRVRPGMKVVPQPGRIVAQAPTAPRPDVVSPPAAQATAAANAE
HHCCCCCEECCCCCCEEEECCCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]