Click here to switch to the map view.

The map label for this gene is xcsE [H]

Identifier: 197104121

GI number: 197104121

Start: 707873

End: 709354

Strand: Direct

Name: xcsE [H]

Synonym: PHZ_c0655

Alternate gene names: 197104121

Gene position: 707873-709354 (Clockwise)

Preceding gene: 197104120

Following gene: 197104122

Centisome position: 17.71

GC content: 74.49

Gene sequence:

>1482_bases
ATGACCGCCGTCCCGCGCCTGGCTTACGCCTTCGCCAAGACGCACGGCGTGCTGCTGCTCGACCTGGGCGAGACGGCCGT
GGTGGGCCTGCGCGAAGGGGCCGATCCGCTCGCGCTGGTGGAGGCCCGGCGGGCCCTGCGCGCGCCGCTGCGGGTCGAGC
CGATGGGGCGGGCCGAGTTCGAGCGGCGGCTCTCGGAAGCCTATGCGGGCGAAGGTCTGGCCGCGCCGGCCGACGACTTC
GACCTGCCGGGCGGGCTGGACAGCCTGGCCGACGAGATCCCCGCCGCCGCCGACCTGCTGGACGCGCAGGACGATGCGCC
CGTGATCCGGCTGATCAACGGCCTGATCGCCGAGGCGGTCCGCCAGGGCGCCTCGGACGTCCACATCGAGCCCTTCGAGG
CCGCGCTGGTCGTGCGGCTGCGGGTGGATGGCGTGCTGAAGGAGGTGCTGAGCCTCTCCCCCCGGATCGCGCCCCTGCTG
GTCAGCCGGGTGAAGGTGATGGCCAGGCTCGACATCGCCGAGAAGCGAGTCCCGCAGGACGGCCGCATCTCGCTGGCGCT
GGGGGGCCGCTCGCTGGACGTGCGGGTCTCGACCCTGCCGGCGCGGGCGGGCGAGCGGGTGGTGATGCGCATCCTCGACC
AGGACCAGGCGCGGCTGGGCCTGGAGGACCTGGGCATGTCGCCGCGGGCGCTGGAGGTGTTCCGCGCCGCGCTCGCCGAG
CCCAACGGCGTGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCACGACGCTCTATGCCGGCCTGACGCTGCTGAA
CGACGCCACCCGCAACATCCTCACCATCGAGGACCCGGTGGAGTACGCGATCCAGGGCCTCGGCCAGACGCAGGTGAACG
CCAAGGTCGGCATGACCTTCGCCGCCGGCCTGCGGGCCATCCTGCGGCAGGACCCGGACGTGGTCATGGTGGGCGAGATC
CGCGACGTGGAGACGGCCCAGATCGCGGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCCACCGTCCACACCAACGA
CGCCGCGGGGGCGATCACCCGCCTGCGGGACGTGGGGGTCGAGCCGTTCCTGCTGGCCTCCACCGTCCGCGCCATCGTCG
CCCAGCGGCTGGTGCGCCGGCTCTGCCCCGACTGCCGCCGGCCGCATGCGGCCGACGCGGTGACCGCCCGGCGGGTCGGG
GTGGCGCGCGGCCATGTGCTGTTCCGCCCCGGGGGCTGCCCGGCGTGCGGGAACACCGGCTACGTCGGCCGGGTCGGGAT
CTACGAGGCGTTGCGCGTGGACGAGCGCATCCGCCGGCTGATCGCCGAGGACGCGGGCGAGGACGCCATCGCGCGGGCCG
CGTTCCCGGGCGGCGGCACGCTGCAGGCCGAGGCCCGGCGCTACGTGGCCGAGGGGATCACCTCGGTCGAGGAGGCGATC
CGGGTCAGCCGCCAGGAAGGCGCCGAGCATGCCGGCGTTTGA

Upstream 100 bases:

>100_bases
CGGCCGCCGACCCGCGCCGCCAGAGCGAGCTGGAGGCCCTGGTGCGGGAGTACCTGCGGGCCGAGCCGCCGACCTCGGCG
CCCCTGCCGGCGGGGCCGAA

Downstream 100 bases:

>100_bases
ATATGTCGCCCTCGACGGCGGCGGGCGCGCCCGCCGCGGCCTCGTCCAGGCGGCCAGCGAGCGCGCGGCCCGCGACCAGC
TTCAGGGCCGCAGGCTCACG

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTAVPRLAYAFAKTHGVLLLDLGETAVVGLREGADPLALVEARRALRAPLRVEPMGRAEFERRLSEAYAGEGLAAPADDF
DLPGGLDSLADEIPAAADLLDAQDDAPVIRLINGLIAEAVRQGASDVHIEPFEAALVVRLRVDGVLKEVLSLSPRIAPLL
VSRVKVMARLDIAEKRVPQDGRISLALGGRSLDVRVSTLPARAGERVVMRILDQDQARLGLEDLGMSPRALEVFRAALAE
PNGVILVTGPTGSGKTTTLYAGLTLLNDATRNILTIEDPVEYAIQGLGQTQVNAKVGMTFAAGLRAILRQDPDVVMVGEI
RDVETAQIAVQASLTGHLVLSTVHTNDAAGAITRLRDVGVEPFLLASTVRAIVAQRLVRRLCPDCRRPHAADAVTARRVG
VARGHVLFRPGGCPACGNTGYVGRVGIYEALRVDERIRRLIAEDAGEDAIARAAFPGGGTLQAEARRYVAEGITSVEEAI
RVSRQEGAEHAGV

Sequences:

>Translated_493_residues
MTAVPRLAYAFAKTHGVLLLDLGETAVVGLREGADPLALVEARRALRAPLRVEPMGRAEFERRLSEAYAGEGLAAPADDF
DLPGGLDSLADEIPAAADLLDAQDDAPVIRLINGLIAEAVRQGASDVHIEPFEAALVVRLRVDGVLKEVLSLSPRIAPLL
VSRVKVMARLDIAEKRVPQDGRISLALGGRSLDVRVSTLPARAGERVVMRILDQDQARLGLEDLGMSPRALEVFRAALAE
PNGVILVTGPTGSGKTTTLYAGLTLLNDATRNILTIEDPVEYAIQGLGQTQVNAKVGMTFAAGLRAILRQDPDVVMVGEI
RDVETAQIAVQASLTGHLVLSTVHTNDAAGAITRLRDVGVEPFLLASTVRAIVAQRLVRRLCPDCRRPHAADAVTARRVG
VARGHVLFRPGGCPACGNTGYVGRVGIYEALRVDERIRRLIAEDAGEDAIARAAFPGGGTLQAEARRYVAEGITSVEEAI
RVSRQEGAEHAGV
>Mature_492_residues
TAVPRLAYAFAKTHGVLLLDLGETAVVGLREGADPLALVEARRALRAPLRVEPMGRAEFERRLSEAYAGEGLAAPADDFD
LPGGLDSLADEIPAAADLLDAQDDAPVIRLINGLIAEAVRQGASDVHIEPFEAALVVRLRVDGVLKEVLSLSPRIAPLLV
SRVKVMARLDIAEKRVPQDGRISLALGGRSLDVRVSTLPARAGERVVMRILDQDQARLGLEDLGMSPRALEVFRAALAEP
NGVILVTGPTGSGKTTTLYAGLTLLNDATRNILTIEDPVEYAIQGLGQTQVNAKVGMTFAAGLRAILRQDPDVVMVGEIR
DVETAQIAVQASLTGHLVLSTVHTNDAAGAITRLRDVGVEPFLLASTVRAIVAQRLVRRLCPDCRRPHAADAVTARRVGV
ARGHVLFRPGGCPACGNTGYVGRVGIYEALRVDERIRRLIAEDAGEDAIARAAFPGGGTLQAEARRYVAEGITSVEEAIR
VSRQEGAEHAGV

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=453, Percent_Identity=51.6556291390728, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786296, Length=386, Percent_Identity=44.559585492228, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI87082188, Length=273, Percent_Identity=38.8278388278388, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 52387; Mature: 52256

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVPRLAYAFAKTHGVLLLDLGETAVVGLREGADPLALVEARRALRAPLRVEPMGRAEF
CCCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
ERRLSEAYAGEGLAAPADDFDLPGGLDSLADEIPAAADLLDAQDDAPVIRLINGLIAEAV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHH
RQGASDVHIEPFEAALVVRLRVDGVLKEVLSLSPRIAPLLVSRVKVMARLDIAEKRVPQD
HCCCCCCEECCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GRISLALGGRSLDVRVSTLPARAGERVVMRILDQDQARLGLEDLGMSPRALEVFRAALAE
CCEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCHHHHCCHHHCCCCCHHHHHHHHHHCC
PNGVILVTGPTGSGKTTTLYAGLTLLNDATRNILTIEDPVEYAIQGLGQTQVNAKVGMTF
CCCEEEEECCCCCCCEEEEEECHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHH
AAGLRAILRQDPDVVMVGEIRDVETAQIAVQASLTGHLVLSTVHTNDAAGAITRLRDVGV
HHHHHHHHHCCCCEEEECCCCCCCHHEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCC
EPFLLASTVRAIVAQRLVRRLCPDCRRPHAADAVTARRVGVARGHVLFRPGGCPACGNTG
CHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
YVGRVGIYEALRVDERIRRLIAEDAGEDAIARAAFPGGGTLQAEARRYVAEGITSVEEAI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RVSRQEGAEHAGV
HHHHHCCCCCCCC
>Mature Secondary Structure 
TAVPRLAYAFAKTHGVLLLDLGETAVVGLREGADPLALVEARRALRAPLRVEPMGRAEF
CCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
ERRLSEAYAGEGLAAPADDFDLPGGLDSLADEIPAAADLLDAQDDAPVIRLINGLIAEAV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHH
RQGASDVHIEPFEAALVVRLRVDGVLKEVLSLSPRIAPLLVSRVKVMARLDIAEKRVPQD
HCCCCCCEECCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GRISLALGGRSLDVRVSTLPARAGERVVMRILDQDQARLGLEDLGMSPRALEVFRAALAE
CCEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCHHHHCCHHHCCCCCHHHHHHHHHHCC
PNGVILVTGPTGSGKTTTLYAGLTLLNDATRNILTIEDPVEYAIQGLGQTQVNAKVGMTF
CCCEEEEECCCCCCCEEEEEECHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHH
AAGLRAILRQDPDVVMVGEIRDVETAQIAVQASLTGHLVLSTVHTNDAAGAITRLRDVGV
HHHHHHHHHCCCCEEEECCCCCCCHHEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCC
EPFLLASTVRAIVAQRLVRRLCPDCRRPHAADAVTARRVGVARGHVLFRPGGCPACGNTG
CHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCC
YVGRVGIYEALRVDERIRRLIAEDAGEDAIARAAFPGGGTLQAEARRYVAEGITSVEEAI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RVSRQEGAEHAGV
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]