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The map label for this gene is pncA [H]

Identifier: 197104113

GI number: 197104113

Start: 695223

End: 695855

Strand: Reverse

Name: pncA [H]

Synonym: PHZ_c0647

Alternate gene names: 197104113

Gene position: 695855-695223 (Counterclockwise)

Preceding gene: 197104114

Following gene: 197104112

Centisome position: 17.41

GC content: 72.51

Gene sequence:

>633_bases
ATGATCCCCATCGCCCCCACCGACGCCCTGATCGTGATCGACGTGCAGAACGACTTCTGTCCCGGCGGTGCCCTGGCCGT
GCCGCGCGGAGACGAGGTGGTCGCCCCGATCAACCGCCTGATGGGCGGCTTCGCGACCGTGGTGCTGACCCAGGACTGGC
ATCCGCCCGGCCACGGCTCGTTCGCCTCGGCGCACCCCGGCCGCCGGCCAATGGAGACCGCCCAGCTCGACTACGGGCTG
CAGGTGCTCTGGCCCGATCACTGCGTCCAGGGTACCCCCGGGGCGGCCTTCCATTCCGGCCTCGACGCCGTGCGCGCCGA
CATGATCCTGCGCAAGGGCGCCGACCCGCGCGTCGACTCCTATTCCGCCTTCCGCGAGAACGACCGCCGCACCGTCACGG
GCCTCGCCGGCTACCTGAAGGACCGCGGCGTGACGCGGGTCTTCCTCGCCGGCCTCGCCTTCGATTTCTGCGTCCTCTGG
TCGACCGAGGACGCCCGGGCCGCCGGGTTCGAGGCGGTCGTCGTCGAGGACGCCTGCCGGGCCCTCGACGTGGACGGCTC
CGAGGCCGAGGCGCGGCGGCGGCTGGGCGAGGCCGACGTGCGGTTCTCACGCGCTGAAGAGCTCAGGCCATGA

Upstream 100 bases:

>100_bases
GGGCGCTGGAGCCCCACGACTATCCGGTGCGGATCGCCGAGCCCCTGACGCGCCTCGCCGCCGAAGTGGACCGCCGGCTC
GCCAAGCAGGTCCAGCCGTC

Downstream 100 bases:

>100_bases
GCGGCCTTCCGTCGACCGTGCGCCTGTTCGCGCTGAACGCCACGGCCGAGCTCGGCGCGGCGATCGCCAGCGCCGCGGGC
CGGCCCCTCACGCCGCACGA

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIPIAPTDALIVIDVQNDFCPGGALAVPRGDEVVAPINRLMGGFATVVLTQDWHPPGHGSFASAHPGRRPMETAQLDYGL
QVLWPDHCVQGTPGAAFHSGLDAVRADMILRKGADPRVDSYSAFRENDRRTVTGLAGYLKDRGVTRVFLAGLAFDFCVLW
STEDARAAGFEAVVVEDACRALDVDGSEAEARRRLGEADVRFSRAEELRP

Sequences:

>Translated_210_residues
MIPIAPTDALIVIDVQNDFCPGGALAVPRGDEVVAPINRLMGGFATVVLTQDWHPPGHGSFASAHPGRRPMETAQLDYGL
QVLWPDHCVQGTPGAAFHSGLDAVRADMILRKGADPRVDSYSAFRENDRRTVTGLAGYLKDRGVTRVFLAGLAFDFCVLW
STEDARAAGFEAVVVEDACRALDVDGSEAEARRRLGEADVRFSRAEELRP
>Mature_210_residues
MIPIAPTDALIVIDVQNDFCPGGALAVPRGDEVVAPINRLMGGFATVVLTQDWHPPGHGSFASAHPGRRPMETAQLDYGL
QVLWPDHCVQGTPGAAFHSGLDAVRADMILRKGADPRVDSYSAFRENDRRTVTGLAGYLKDRGVTRVFLAGLAFDFCVLW
STEDARAAGFEAVVVEDACRALDVDGSEAEARRRLGEADVRFSRAEELRP

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=183, Percent_Identity=44.8087431693989, Blast_Score=161, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI193206735, Length=204, Percent_Identity=35.2941176470588, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543130, Length=204, Percent_Identity=35.2941176470588, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71999339, Length=220, Percent_Identity=31.3636363636364, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=33.6787564766839, Blast_Score=103, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648247, Length=220, Percent_Identity=35, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22717; Mature: 22717

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIAPTDALIVIDVQNDFCPGGALAVPRGDEVVAPINRLMGGFATVVLTQDWHPPGHGS
CCCCCCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
FASAHPGRRPMETAQLDYGLQVLWPDHCVQGTPGAAFHSGLDAVRADMILRKGADPRVDS
CCCCCCCCCCCHHHHHCCCEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
YSAFRENDRRTVTGLAGYLKDRGVTRVFLAGLAFDFCVLWSTEDARAAGFEAVVVEDACR
HHHHHCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCHHCCCEEEEEHHHHH
ALDVDGSEAEARRRLGEADVRFSRAEELRP
HCCCCCCHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPIAPTDALIVIDVQNDFCPGGALAVPRGDEVVAPINRLMGGFATVVLTQDWHPPGHGS
CCCCCCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
FASAHPGRRPMETAQLDYGLQVLWPDHCVQGTPGAAFHSGLDAVRADMILRKGADPRVDS
CCCCCCCCCCCHHHHHCCCEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
YSAFRENDRRTVTGLAGYLKDRGVTRVFLAGLAFDFCVLWSTEDARAAGFEAVVVEDACR
HHHHHCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCHHCCCEEEEEHHHHH
ALDVDGSEAEARRRLGEADVRFSRAEELRP
HCCCCCCHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]