Click here to switch to the map view.

The map label for this gene is crp [H]

Identifier: 197104084

GI number: 197104084

Start: 663680

End: 664381

Strand: Reverse

Name: crp [H]

Synonym: PHZ_c0618

Alternate gene names: 197104084

Gene position: 664381-663680 (Counterclockwise)

Preceding gene: 197104086

Following gene: 197104081

Centisome position: 16.63

GC content: 72.36

Gene sequence:

>702_bases
ATGACCGTCGAGCTCGATCCGTCCCTTCTCGAGGGGGCCGACATGTTCGACGTGCTGGACCCCGACGGGCGCCGAGAGGT
GCTCCAGGCGGGCCTCGTGCGCCGGCTGCCGCAGGGCAAGATGGTGTTCGCCCAGGGCGATCCCGGCGCCACCTGCCACA
CCCTGCTCCACGGGCGGGTGAAGATCGTCCAGACCCGGCCCGACGGGGCCCAGTCGGTGATCCGCTTCATCGGCCCCGGC
GAGATGTACGGCACGGTGGCCGCGCTGATGGACAAGCCCTTCCCGGCCGATGCGGTGGCCGTGCTCGACAGCGTCGAGAT
CTACTGGCCGGTGGCGGTCATGCGCCAGCTGATGACCCGCTTCCCGCAGGTGGCGATGCGCTCGGCCGCCTCGGCGGGCG
AGCGCCTGTTCGAGCTGCAGAGCCGGGTCGGCGAGCTGGCCGGGGAGAAGGTGGAGCAGCGGATCGCCCGCGCCCTGGTG
CGCCTGGTCCGCCAGGCGGGCCGGCGCACGCCCGAAGGCGTCGAGATCGACTTCCCGATCACTCGCCAGGAGCTCGCCGA
GATGGCCGGCTCGACGCTCCACACCGTCAGCCGCACGCTCTCGGCCTGGGACGACCGGGAGATCACCGCCAGCTCGCGCC
GCCACATCGTGGTGCGCAAGCTGCACGTCCTCCTGGACCTGGCCGAGCAGGGCGCGGGCTGA

Upstream 100 bases:

>100_bases
CGGAAACCCGCATCGCCCCCTAAGTATTTACCCCAGGTCGACGCCCGTTCTCCGCTTCGGTAATCGGGCCCGGCCCATCC
ATGACAGCGGAACCGCCATG

Downstream 100 bases:

>100_bases
CGTCCGCGCCCCCTCTTGGGCCCCTTCTACTTGCCCCTCTACTTGAGCGCCGCGAAGCTGGCCGTCAGCCGCTTCTTGGC
CATCGGCGGGGTCTTGGCGA

Product: transcriptional regulator, Crp/Fnr family

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTVELDPSLLEGADMFDVLDPDGRREVLQAGLVRRLPQGKMVFAQGDPGATCHTLLHGRVKIVQTRPDGAQSVIRFIGPG
EMYGTVAALMDKPFPADAVAVLDSVEIYWPVAVMRQLMTRFPQVAMRSAASAGERLFELQSRVGELAGEKVEQRIARALV
RLVRQAGRRTPEGVEIDFPITRQELAEMAGSTLHTVSRTLSAWDDREITASSRRHIVVRKLHVLLDLAEQGAG

Sequences:

>Translated_233_residues
MTVELDPSLLEGADMFDVLDPDGRREVLQAGLVRRLPQGKMVFAQGDPGATCHTLLHGRVKIVQTRPDGAQSVIRFIGPG
EMYGTVAALMDKPFPADAVAVLDSVEIYWPVAVMRQLMTRFPQVAMRSAASAGERLFELQSRVGELAGEKVEQRIARALV
RLVRQAGRRTPEGVEIDFPITRQELAEMAGSTLHTVSRTLSAWDDREITASSRRHIVVRKLHVLLDLAEQGAG
>Mature_232_residues
TVELDPSLLEGADMFDVLDPDGRREVLQAGLVRRLPQGKMVFAQGDPGATCHTLLHGRVKIVQTRPDGAQSVIRFIGPGE
MYGTVAALMDKPFPADAVAVLDSVEIYWPVAVMRQLMTRFPQVAMRSAASAGERLFELQSRVGELAGEKVEQRIARALVR
LVRQAGRRTPEGVEIDFPITRQELAEMAGSTLHTVSRTLSAWDDREITASSRRHIVVRKLHVLLDLAEQGAG

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=190, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25632; Mature: 25501

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVELDPSLLEGADMFDVLDPDGRREVLQAGLVRRLPQGKMVFAQGDPGATCHTLLHGRV
CEEECCHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCE
KIVQTRPDGAQSVIRFIGPGEMYGTVAALMDKPFPADAVAVLDSVEIYWPVAVMRQLMTR
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FPQVAMRSAASAGERLFELQSRVGELAGEKVEQRIARALVRLVRQAGRRTPEGVEIDFPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
TRQELAEMAGSTLHTVSRTLSAWDDREITASSRRHIVVRKLHVLLDLAEQGAG
CHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVELDPSLLEGADMFDVLDPDGRREVLQAGLVRRLPQGKMVFAQGDPGATCHTLLHGRV
EEECCHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCE
KIVQTRPDGAQSVIRFIGPGEMYGTVAALMDKPFPADAVAVLDSVEIYWPVAVMRQLMTR
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FPQVAMRSAASAGERLFELQSRVGELAGEKVEQRIARALVRLVRQAGRRTPEGVEIDFPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCC
TRQELAEMAGSTLHTVSRTLSAWDDREITASSRRHIVVRKLHVLLDLAEQGAG
CHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]