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The map label for this gene is dagK [H]

Identifier: 197104072

GI number: 197104072

Start: 649158

End: 650036

Strand: Reverse

Name: dagK [H]

Synonym: PHZ_c0606

Alternate gene names: 197104072

Gene position: 650036-649158 (Counterclockwise)

Preceding gene: 197104073

Following gene: 197104070

Centisome position: 16.27

GC content: 76.22

Gene sequence:

>879_bases
ATGGCGCGGCGCGGCCTTCTCATCCGCAACCGATCCAAGCCGGACGACTGGGTCGCCGCGGTGCGCGCGCGGCTGGCCGA
CGCCGGCATCGAGCTCGTCGACCGCCCCTGCGGCTCGGGCGAGGAGGCCTGCCGCATCATCGCCGCCGAGGGAAAGGCCG
AGGGAGAGGGCGCCGACCTCGTCGGGGTGGCGGGCGGCGACGGCACGCTGAACGGCGCCGCCCCGGCCCTGCTGGAGCTC
GGCAAGCCGCTGGGCGTCCTGCCGCTGGGCACCGCCAACGACCTGGCCCGCACCCTCGGCCTGCCCCTCGACCCGCTCGC
CGCCGCCGAGGTCATCGCCGCCGGGCAGACGCGTAGGATCGACCTCGGGCTCGCCAACGGCCGGCCGTTCTTCAACGTCG
CCAGCCTCGGCCTCGCCGCCGAGGTGGCCCAGACCCTGGAGGGCGCCGGCAAGCCCCTCGGTCGGCTCTCCTACGCCCTG
GCGGCGCTGAAGGTGCTGCTCCGCGCCCGGCCGTTCCGGGCCGCCATCGCCGCCGGCGACCGGCTCGTCCGCACCCGCAG
CTACCAGATCGCCGTCGGCAACGGCCGCTACCACGGCGGCGGCCTGGCCGTGCGCGAGGACGCCAGCATCGAGAGCGGCC
GGCTCGTCCTCTACAGCCTCGAGCCGGGCAGCCTCTGGAAGGTGGTCCTGCTCGCGCCGCTGTTCCGCCGCGGCCGCCAC
GTGCGCTGGCGGCAGGTGCGGACCGCCAGCGCGCGGACGATCGAGATCCGCACGCCCTCGCCGATGCCGGTCAACCTCGA
CGGCGACCTCGCCACGGAGACCCCGCTCCGGCTGGAGGAGCTGCCGGGCGCGATCGAGGTGTTCGTCCCCGACGCCTAG

Upstream 100 bases:

>100_bases
AACCGCGACTACTCCATGGCCCGCACGGCCTTCATCGACGCCATCCGCGCGGCGGCGACGACGGAGTGAGCCTTCCCGCG
CCTTAGCCGTTGAGGCTCCC

Downstream 100 bases:

>100_bases
GCCGCGACCGGCGCCGGCGCCTTCTCCCACTGCGACCATAGGCGCCTGGCCTCGGCCTTGGCGGGCCCGACGGAGGCGTC
CTTCACATGGCCGAAGCCGC

Product: methylglyoxal synthase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MARRGLLIRNRSKPDDWVAAVRARLADAGIELVDRPCGSGEEACRIIAAEGKAEGEGADLVGVAGGDGTLNGAAPALLEL
GKPLGVLPLGTANDLARTLGLPLDPLAAAEVIAAGQTRRIDLGLANGRPFFNVASLGLAAEVAQTLEGAGKPLGRLSYAL
AALKVLLRARPFRAAIAAGDRLVRTRSYQIAVGNGRYHGGGLAVREDASIESGRLVLYSLEPGSLWKVVLLAPLFRRGRH
VRWRQVRTASARTIEIRTPSPMPVNLDGDLATETPLRLEELPGAIEVFVPDA

Sequences:

>Translated_292_residues
MARRGLLIRNRSKPDDWVAAVRARLADAGIELVDRPCGSGEEACRIIAAEGKAEGEGADLVGVAGGDGTLNGAAPALLEL
GKPLGVLPLGTANDLARTLGLPLDPLAAAEVIAAGQTRRIDLGLANGRPFFNVASLGLAAEVAQTLEGAGKPLGRLSYAL
AALKVLLRARPFRAAIAAGDRLVRTRSYQIAVGNGRYHGGGLAVREDASIESGRLVLYSLEPGSLWKVVLLAPLFRRGRH
VRWRQVRTASARTIEIRTPSPMPVNLDGDLATETPLRLEELPGAIEVFVPDA
>Mature_291_residues
ARRGLLIRNRSKPDDWVAAVRARLADAGIELVDRPCGSGEEACRIIAAEGKAEGEGADLVGVAGGDGTLNGAAPALLELG
KPLGVLPLGTANDLARTLGLPLDPLAAAEVIAAGQTRRIDLGLANGRPFFNVASLGLAAEVAQTLEGAGKPLGRLSYALA
ALKVLLRARPFRAAIAAGDRLVRTRSYQIAVGNGRYHGGGLAVREDASIESGRLVLYSLEPGSLWKVVLLAPLFRRGRHV
RWRQVRTASARTIEIRTPSPMPVNLDGDLATETPLRLEELPGAIEVFVPDA

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=240, Percent_Identity=28.75, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 30715; Mature: 30584

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRGLLIRNRSKPDDWVAAVRARLADAGIELVDRPCGSGEEACRIIAAEGKAEGEGADL
CCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHEEEEEECCCCCCCCCCE
VGVAGGDGTLNGAAPALLELGKPLGVLPLGTANDLARTLGLPLDPLAAAEVIAAGQTRRI
EEEECCCCCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEE
DLGLANGRPFFNVASLGLAAEVAQTLEGAGKPLGRLSYALAALKVLLRARPFRAAIAAGD
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCC
RLVRTRSYQIAVGNGRYHGGGLAVREDASIESGRLVLYSLEPGSLWKVVLLAPLFRRGRH
CEEEEEEEEEEECCCEEECCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCC
VRWRQVRTASARTIEIRTPSPMPVNLDGDLATETPLRLEELPGAIEVFVPDA
CEEHHEECCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHCCCEEEEEECCC
>Mature Secondary Structure 
ARRGLLIRNRSKPDDWVAAVRARLADAGIELVDRPCGSGEEACRIIAAEGKAEGEGADL
CCCCCEEECCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHEEEEEECCCCCCCCCCE
VGVAGGDGTLNGAAPALLELGKPLGVLPLGTANDLARTLGLPLDPLAAAEVIAAGQTRRI
EEEECCCCCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEE
DLGLANGRPFFNVASLGLAAEVAQTLEGAGKPLGRLSYALAALKVLLRARPFRAAIAAGD
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCC
RLVRTRSYQIAVGNGRYHGGGLAVREDASIESGRLVLYSLEPGSLWKVVLLAPLFRRGRH
CEEEEEEEEEEECCCEEECCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCC
VRWRQVRTASARTIEIRTPSPMPVNLDGDLATETPLRLEELPGAIEVFVPDA
CEEHHEECCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]