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The map label for this gene is 197104002

Identifier: 197104002

GI number: 197104002

Start: 563353

End: 564708

Strand: Direct

Name: 197104002

Synonym: PHZ_c0536

Alternate gene names: NA

Gene position: 563353-564708 (Clockwise)

Preceding gene: 197104001

Following gene: 197104004

Centisome position: 14.1

GC content: 70.43

Gene sequence:

>1356_bases
ATGGCCGGTGTCGCCTGCGACGATCGCCCGCTCGTGTTCGCGGACCTGGTCCGCTGGTTCGAGGCGGGCGCCAAGCCCGC
CGCCGACTGGCGGGTCGGGGCCGAGCACGAGAAGTTCGTCTTCAAGCTGGGCTCGCACGAGCCGGTCCCCTACGAGCCCG
GCGGCATCAAGGCGCTGCTCGACGGCCTCACCCGCTTCGGCTGGAAGCCTGTCCTCGAGGGCGAGAACGTCATCGCGCTG
GAGCGCGGCATGGCCAGCGTCAGCCTCGAGCCCGGCGGCCAGTTCGAGCTGTCCGGCGCCCCGCTCGAGACCATCCACGA
CATCTGCGAGGAGACCGGCCAGCACCTGCAGGAGGTGAAGGTCGTGGCCGACGAGCTGGGCCTGGGCTTCCTGGGGCTGG
GCTTCACCCCCACCTGGACCCGCGCGCAGGTCCCGGTGATGCCCAAGGGCCGCTACGGCATCATGCGGGCCTACATGCCC
AAGGTCGGCTCCCTCGGCCTCGACATGATGTTCCGCACCTGCACGGTGCAGGCGAACCTCGACTTCGGCTCGGAAGCCGA
CATGGTCGCCAAGTTCCGCGCCTCGCTGGCGCTGCAGCCGATCGCCACCGCGCTGTTCGCCAACTCGCCGTTCAAGGAAG
GCAAGCCGTCGGGCCTGCTCTCCACGCGCGCGAACGTGTGGACCGACACCGATCCCGACCGCACGGGGATGCTGGATTTC
GTGTTCGAGGACGGCTTCGGATTCGAGACCTACGCGAACTACGCCCTCGACGTGCCGATGTATTTCGTCAAGCGCGACGG
CCGGTACATCGACGTGGCCGGCCGCTCGTTCCGCGACTTCATGGAGGGGCGGCTGCCCGAGCTGCCGGGCGAGCGGCCGA
CGATCAAGGACTGGGCCGACCACACCACCACCATCTTCCCCGAGGTGCGGCTGAAGCAGTACCTGGAGATGCGCGGCGCC
GACTCCGGGCCGTGGAGCCGGCTGTGCGCCCTGCCGGCCCTCTGGATGGGGATCCTCTACGACTCCGCCGCGCTCGAGGC
GGCCTGGGACCTGGCCAAGGGGTGGGCCATCGAGGACCACGAGCGCCTGCGGGCCGACGTGGCGCGCCTGGGCCTGAAGG
CGCAGGTCGGCGGCCGCTCGGTGCAGGACGTGGCCAAGGACATGCTGGCCATCGCCCGCGAGGGGCTGAAGCGCCGCAAC
CGCCTGTCGGGCGGCCTGGTGGACGAGAGCGGCTACCTTGCCGAGCTGGAGCAGATCGCCGACAGCGGCGTCACGCCCGC
CGAGCGCCTGCTCGAACTGTACGAGACCCGCTGGAACGGCGACGCCGGCCGGGTCTTCGACGACTTCGCCTACTAG

Upstream 100 bases:

>100_bases
AGCAACGGCCGAATTGCCCCCACCTAGCTGAACGGCGTAAAGATCACGGCTGCGCGGGACCTCGCCCGCCATGATCCGGG
TTGGGCTGGGGAGGTTTTGG

Downstream 100 bases:

>100_bases
GGCGCGCGCTCAGCGCAGGCCGCCGCCGGGGCCCTCCACGTTGGGCTCCCAGTGTTCGCGCTGGCTGGTCCCGGCGCCCG
AGTCGGTGTGCTGGACGACC

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAGVACDDRPLVFADLVRWFEAGAKPAADWRVGAEHEKFVFKLGSHEPVPYEPGGIKALLDGLTRFGWKPVLEGENVIAL
ERGMASVSLEPGGQFELSGAPLETIHDICEETGQHLQEVKVVADELGLGFLGLGFTPTWTRAQVPVMPKGRYGIMRAYMP
KVGSLGLDMMFRTCTVQANLDFGSEADMVAKFRASLALQPIATALFANSPFKEGKPSGLLSTRANVWTDTDPDRTGMLDF
VFEDGFGFETYANYALDVPMYFVKRDGRYIDVAGRSFRDFMEGRLPELPGERPTIKDWADHTTTIFPEVRLKQYLEMRGA
DSGPWSRLCALPALWMGILYDSAALEAAWDLAKGWAIEDHERLRADVARLGLKAQVGGRSVQDVAKDMLAIAREGLKRRN
RLSGGLVDESGYLAELEQIADSGVTPAERLLELYETRWNGDAGRVFDDFAY

Sequences:

>Translated_451_residues
MAGVACDDRPLVFADLVRWFEAGAKPAADWRVGAEHEKFVFKLGSHEPVPYEPGGIKALLDGLTRFGWKPVLEGENVIAL
ERGMASVSLEPGGQFELSGAPLETIHDICEETGQHLQEVKVVADELGLGFLGLGFTPTWTRAQVPVMPKGRYGIMRAYMP
KVGSLGLDMMFRTCTVQANLDFGSEADMVAKFRASLALQPIATALFANSPFKEGKPSGLLSTRANVWTDTDPDRTGMLDF
VFEDGFGFETYANYALDVPMYFVKRDGRYIDVAGRSFRDFMEGRLPELPGERPTIKDWADHTTTIFPEVRLKQYLEMRGA
DSGPWSRLCALPALWMGILYDSAALEAAWDLAKGWAIEDHERLRADVARLGLKAQVGGRSVQDVAKDMLAIAREGLKRRN
RLSGGLVDESGYLAELEQIADSGVTPAERLLELYETRWNGDAGRVFDDFAY
>Mature_450_residues
AGVACDDRPLVFADLVRWFEAGAKPAADWRVGAEHEKFVFKLGSHEPVPYEPGGIKALLDGLTRFGWKPVLEGENVIALE
RGMASVSLEPGGQFELSGAPLETIHDICEETGQHLQEVKVVADELGLGFLGLGFTPTWTRAQVPVMPKGRYGIMRAYMPK
VGSLGLDMMFRTCTVQANLDFGSEADMVAKFRASLALQPIATALFANSPFKEGKPSGLLSTRANVWTDTDPDRTGMLDFV
FEDGFGFETYANYALDVPMYFVKRDGRYIDVAGRSFRDFMEGRLPELPGERPTIKDWADHTTTIFPEVRLKQYLEMRGAD
SGPWSRLCALPALWMGILYDSAALEAAWDLAKGWAIEDHERLRADVARLGLKAQVGGRSVQDVAKDMLAIAREGLKRRNR
LSGGLVDESGYLAELEQIADSGVTPAERLLELYETRWNGDAGRVFDDFAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 49888; Mature: 49757

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVACDDRPLVFADLVRWFEAGAKPAADWRVGAEHEKFVFKLGSHEPVPYEPGGIKALL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCHHHHHH
DGLTRFGWKPVLEGENVIALERGMASVSLEPGGQFELSGAPLETIHDICEETGQHLQEVK
HHHHHHCCEEECCCCCEEEEECCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
VVADELGLGFLGLGFTPTWTRAQVPVMPKGRYGIMRAYMPKVGSLGLDMMFRTCTVQANL
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHEEEEEECC
DFGSEADMVAKFRASLALQPIATALFANSPFKEGKPSGLLSTRANVWTDTDPDRTGMLDF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEE
VFEDGFGFETYANYALDVPMYFVKRDGRYIDVAGRSFRDFMEGRLPELPGERPTIKDWAD
EEECCCCHHHHHCHHCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHC
HTTTIFPEVRLKQYLEMRGADSGPWSRLCALPALWMGILYDSAALEAAWDLAKGWAIEDH
CCEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ERLRADVARLGLKAQVGGRSVQDVAKDMLAIAREGLKRRNRLSGGLVDESGYLAELEQIA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
DSGVTPAERLLELYETRWNGDAGRVFDDFAY
HCCCCHHHHHHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
AGVACDDRPLVFADLVRWFEAGAKPAADWRVGAEHEKFVFKLGSHEPVPYEPGGIKALL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCHHHHHH
DGLTRFGWKPVLEGENVIALERGMASVSLEPGGQFELSGAPLETIHDICEETGQHLQEVK
HHHHHHCCEEECCCCCEEEEECCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
VVADELGLGFLGLGFTPTWTRAQVPVMPKGRYGIMRAYMPKVGSLGLDMMFRTCTVQANL
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHEEEEEECC
DFGSEADMVAKFRASLALQPIATALFANSPFKEGKPSGLLSTRANVWTDTDPDRTGMLDF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEE
VFEDGFGFETYANYALDVPMYFVKRDGRYIDVAGRSFRDFMEGRLPELPGERPTIKDWAD
EEECCCCHHHHHCHHCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHC
HTTTIFPEVRLKQYLEMRGADSGPWSRLCALPALWMGILYDSAALEAAWDLAKGWAIEDH
CCEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ERLRADVARLGLKAQVGGRSVQDVAKDMLAIAREGLKRRNRLSGGLVDESGYLAELEQIA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
DSGVTPAERLLELYETRWNGDAGRVFDDFAY
HCCCCHHHHHHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA