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The map label for this gene is rsmE [H]

Identifier: 197104001

GI number: 197104001

Start: 562534

End: 563253

Strand: Direct

Name: rsmE [H]

Synonym: PHZ_c0535

Alternate gene names: 197104001

Gene position: 562534-563253 (Clockwise)

Preceding gene: 197103998

Following gene: 197104002

Centisome position: 14.08

GC content: 76.39

Gene sequence:

>720_bases
ATGATCCGTCTGTTCGTGACGCACGACCTTTCGGCCGGCGCCGAGCTCGCCCTGGACGAGGGCCAGAGCCGGTATCTCGC
CGCCGTCATGCGGGCCAAGGCCGGGGACGAGCTGCGGGTGTTCAACGGCCGCGACGGCGAGTGGCGCGCGCTGGTGGCGG
CCGTCGGCAAGCGCGCGGTGACGCTGCAGGTGATCGACCCCGCCCGCCCGCAGGCCCCGGGGCCCGACCTCGACCTCGTC
GTCGCGCTGGTCAAGCGCGCGCCGCTGGAGACCATCGTCGAGAAGGCGGCCGAGCTGGGCGCCCGGCGCATCCGGCCCGT
GGTGACCGAGCGGACCAACGCCGACCACACCCGGGTGGAGCGCCTGCGGGCCATCGCCACCGAGGCCTCGGAACAGACCG
GCCGGCTGGACGTGCCCGAGGTGACCGAGCCCGTGAAGCTGGAGCGGCTGCTCGCCGAATGGGAGCCCGGCCGGCGGCTG
CTGTTCTGCGACGAGGCGGGCGACGCCCGGCCCGTGCTCGAGGCGCTGGAGGGCGCGGCCGGCCCCTGGGCCATCCTCAT
CGGACCCGAGGGCGGCTTCTCCCCGCGCGAGCGGGAGGCGCTGCGGGCGCTGCCCTACGCCGTCCCGGCCAGCCTGGGGC
CCCGGATCCTGCGGGCCGACACGGCGGCGATCAGCGCGCTCGCGCTGTGGCAGGCCGCGGCCGGCGACTGGCGCTCTTGA

Upstream 100 bases:

>100_bases
CGAGGGCGACCTGCAGGGCCTCGAGTTCGCGGTCGTAGTCGGACTTGGGCATGGAGCTGAGCCTATGCGGGGATCGGCGG
GGGAGGCTAGAACGCGCGGC

Downstream 100 bases:

>100_bases
GCAACGGCCGAATTGCCCCCACCTAGCTGAACGGCGTAAAGATCACGGCTGCGCGGGACCTCGCCCGCCATGATCCGGGT
TGGGCTGGGGAGGTTTTGGA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIRLFVTHDLSAGAELALDEGQSRYLAAVMRAKAGDELRVFNGRDGEWRALVAAVGKRAVTLQVIDPARPQAPGPDLDLV
VALVKRAPLETIVEKAAELGARRIRPVVTERTNADHTRVERLRAIATEASEQTGRLDVPEVTEPVKLERLLAEWEPGRRL
LFCDEAGDARPVLEALEGAAGPWAILIGPEGGFSPREREALRALPYAVPASLGPRILRADTAAISALALWQAAAGDWRS

Sequences:

>Translated_239_residues
MIRLFVTHDLSAGAELALDEGQSRYLAAVMRAKAGDELRVFNGRDGEWRALVAAVGKRAVTLQVIDPARPQAPGPDLDLV
VALVKRAPLETIVEKAAELGARRIRPVVTERTNADHTRVERLRAIATEASEQTGRLDVPEVTEPVKLERLLAEWEPGRRL
LFCDEAGDARPVLEALEGAAGPWAILIGPEGGFSPREREALRALPYAVPASLGPRILRADTAAISALALWQAAAGDWRS
>Mature_239_residues
MIRLFVTHDLSAGAELALDEGQSRYLAAVMRAKAGDELRVFNGRDGEWRALVAAVGKRAVTLQVIDPARPQAPGPDLDLV
VALVKRAPLETIVEKAAELGARRIRPVVTERTNADHTRVERLRAIATEASEQTGRLDVPEVTEPVKLERLLAEWEPGRRL
LFCDEAGDARPVLEALEGAAGPWAILIGPEGGFSPREREALRALPYAVPASLGPRILRADTAAISALALWQAAAGDWRS

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=241, Percent_Identity=27.8008298755187, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25867; Mature: 25867

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLFVTHDLSAGAELALDEGQSRYLAAVMRAKAGDELRVFNGRDGEWRALVAAVGKRAV
CEEEEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCEE
TLQVIDPARPQAPGPDLDLVVALVKRAPLETIVEKAAELGARRIRPVVTERTNADHTRVE
EEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHH
RLRAIATEASEQTGRLDVPEVTEPVKLERLLAEWEPGRRLLFCDEAGDARPVLEALEGAA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCC
GPWAILIGPEGGFSPREREALRALPYAVPASLGPRILRADTAAISALALWQAAAGDWRS
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIRLFVTHDLSAGAELALDEGQSRYLAAVMRAKAGDELRVFNGRDGEWRALVAAVGKRAV
CEEEEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCEE
TLQVIDPARPQAPGPDLDLVVALVKRAPLETIVEKAAELGARRIRPVVTERTNADHTRVE
EEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHH
RLRAIATEASEQTGRLDVPEVTEPVKLERLLAEWEPGRRLLFCDEAGDARPVLEALEGAA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCC
GPWAILIGPEGGFSPREREALRALPYAVPASLGPRILRADTAAISALALWQAAAGDWRS
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]