Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 197103973

GI number: 197103973

Start: 532937

End: 533629

Strand: Direct

Name: phoB [H]

Synonym: PHZ_c0507

Alternate gene names: 197103973

Gene position: 532937-533629 (Clockwise)

Preceding gene: 197103972

Following gene: 197103974

Centisome position: 13.34

GC content: 71.28

Gene sequence:

>693_bases
GTGACGACGCCACGCATACTGATCGTCGAGGACGAGGACTCGCTCGCGACGCTGCTTCAGTACAACCTCGAGAAGGAGGG
CTACGAGGTCGCCGCCGCCGGCGACGGCGAGGAGGCCCTGCTGCAGGTGCAGGAGCGCCTGCCCGACCTCATCGTGCTGG
ACTGGATGCTGCCCAAGGTGTCGGGCATCGAGGTGTGCCGGCGGCTGCGTCAGCGTCCCGAGAGCCGCAACGTGCCGATC
ATCATGCTGACGGCGCGGGGCGAGGAGACCGACCGCATCCGCGGCCTGGACACCGGCGCCGACGACTATGTGGTCAAGCC
CTTCGCCATGAGCGAGCTGGCGGCCCGCATCCGCGCGGTGCTGCGCCGGCTGCGCCCGGGCCTGGCCGAGGACCGCGTCC
GCTGCGGCGACATCATCATCGACCGGGTCGCCCACCGCGTGAAGCGGAACGGCGCGGAGGTGCACCTGGGCCCCACCGAG
TTCCGGCTGCTGGACCACTTCATGCAGCATCCCGGGCGGGTGTTCTCGCGCGAGCAGCTGCTGGACGCCGTCTGGGGGTC
GGACGTCTATGTCGAGGCCCGGACCGTCGACGTGCACATCGGGCGCCTGCGCAAGGCCCTGAACGCGGCGGACGCGGGCG
ACCCGATCCGCACGGTCCGCTCGGCCGGCTACTCGCTGGACGTCGACGCCTGA

Upstream 100 bases:

>100_bases
AACATCGCCGAGCTCATCCACTACGAGATCACGGGCGAGGAGATGATCTCCCAGGCCCGGCCGAAGACCGATCCACTGGA
AGCCTGACGGGGGCCGCTAA

Downstream 100 bases:

>100_bases
GCGCTTGACCGGAGGGCGGCGGACCGCCAAAGCCGCCGTCCATGAACATCCTGCTGGTTGGCTCGGGCGGCCGCGAACAC
GCGCTCGCCTGGAAGATCGC

Product: phosphate regulon response regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTTPRILIVEDEDSLATLLQYNLEKEGYEVAAAGDGEEALLQVQERLPDLIVLDWMLPKVSGIEVCRRLRQRPESRNVPI
IMLTARGEETDRIRGLDTGADDYVVKPFAMSELAARIRAVLRRLRPGLAEDRVRCGDIIIDRVAHRVKRNGAEVHLGPTE
FRLLDHFMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKALNAADAGDPIRTVRSAGYSLDVDA

Sequences:

>Translated_230_residues
MTTPRILIVEDEDSLATLLQYNLEKEGYEVAAAGDGEEALLQVQERLPDLIVLDWMLPKVSGIEVCRRLRQRPESRNVPI
IMLTARGEETDRIRGLDTGADDYVVKPFAMSELAARIRAVLRRLRPGLAEDRVRCGDIIIDRVAHRVKRNGAEVHLGPTE
FRLLDHFMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKALNAADAGDPIRTVRSAGYSLDVDA
>Mature_229_residues
TTPRILIVEDEDSLATLLQYNLEKEGYEVAAAGDGEEALLQVQERLPDLIVLDWMLPKVSGIEVCRRLRQRPESRNVPII
MLTARGEETDRIRGLDTGADDYVVKPFAMSELAARIRAVLRRLRPGLAEDRVRCGDIIIDRVAHRVKRNGAEVHLGPTEF
RLLDHFMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKALNAADAGDPIRTVRSAGYSLDVDA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=50, Blast_Score=231, Evalue=3e-62,
Organism=Escherichia coli, GI1788394, Length=231, Percent_Identity=41.1255411255411, Blast_Score=163, Evalue=9e-42,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=40.4444444444444, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI1786911, Length=213, Percent_Identity=42.2535211267606, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=37.5, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=37.719298245614, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=40.3508771929825, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=35.1351351351351, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790863, Length=242, Percent_Identity=36.7768595041322, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=37.8378378378378, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=34.2342342342342, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=33.0357142857143, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1787229, Length=233, Percent_Identity=36.9098712446352, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=31.8965517241379, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=36.697247706422, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=33.6206896551724, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25857; Mature: 25726

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPRILIVEDEDSLATLLQYNLEKEGYEVAAAGDGEEALLQVQERLPDLIVLDWMLPKV
CCCCEEEEEECCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEHHHCCCC
SGIEVCRRLRQRPESRNVPIIMLTARGEETDRIRGLDTGADDYVVKPFAMSELAARIRAV
CHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCEEECHHHHHHHHHHHHHH
LRRLRPGLAEDRVRCGDIIIDRVAHRVKRNGAEVHLGPTEFRLLDHFMQHPGRVFSREQL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHH
LDAVWGSDVYVEARTVDVHIGRLRKALNAADAGDPIRTVRSAGYSLDVDA
HHHHCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TTPRILIVEDEDSLATLLQYNLEKEGYEVAAAGDGEEALLQVQERLPDLIVLDWMLPKV
CCCEEEEEECCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEHHHCCCC
SGIEVCRRLRQRPESRNVPIIMLTARGEETDRIRGLDTGADDYVVKPFAMSELAARIRAV
CHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCCCCCCCEEECHHHHHHHHHHHHHH
LRRLRPGLAEDRVRCGDIIIDRVAHRVKRNGAEVHLGPTEFRLLDHFMQHPGRVFSREQL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCHHHHHHHH
LDAVWGSDVYVEARTVDVHIGRLRKALNAADAGDPIRTVRSAGYSLDVDA
HHHHCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]