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The map label for this gene is purD

Identifier: 197103974

GI number: 197103974

Start: 533671

End: 534942

Strand: Direct

Name: purD

Synonym: PHZ_c0508

Alternate gene names: 197103974

Gene position: 533671-534942 (Clockwise)

Preceding gene: 197103973

Following gene: 197103976

Centisome position: 13.35

GC content: 73.51

Gene sequence:

>1272_bases
ATGAACATCCTGCTGGTTGGCTCGGGCGGCCGCGAACACGCGCTCGCCTGGAAGATCGCGGCCTCGCCGCTGGTGAAGCG
CCTGGTGGCCGCGCCGGGCAATCCCGGCATGGCCGAGCAGTGCGAGCTGCGCGCCGTGGGGCCCACCGACGTGCCGGCCC
TGGTGGCGCTGGCCAAGGAGATCGCCGCCGACCTCGTGGTGATCGGCCCGGAATCGTCCGTGGATGCGGGCCTCGCCGAC
CAGCTGCAGGCCGAGGGGATCCCCTGCTTCGGTCCGACGGCGGGCGCGGGGCAGCTGGAGTCCTCCAAGGCCTTCACCAA
GGCCTTCGCCGACCGCTACGGCCTGCCCACCGCCGCCTACCGCGTCTGCGAGAGCGCCGAGGCGGCCCGCGCGGCGCTGG
AGCTGTTCGAGCCGCCCTATGTGATCAAGGCCGACGGCCTGGCGGCCGGCAAGGGCGTCACCGTGGCCCAGGACCGCGGC
GAGGCCGAGGCCGCCATCGACGACGCCTTCGGCGGCCGCTTCGGCGCGGCCGGAGCCCGCGTGGTGGTCGAGGAGTTCCT
GGAGGGCGAGATCGGCTCGCTGTTCGCCCTGTGCGACGGCGAAGCCTCCATGCTGTTCGGCTGGGCCCAGGACCACAAGC
GCGTCTTCGACGGCGAGCAGGGACCGAACACGGGCGGGATGGGCACCTATTCCCCTGCGCCGGTCTTCACGCCCGAGCTG
GTGGAGCAGGTCCGCACGCGGCTGGTGGAGCCCGCTTTCGCCGGCATCGCCGCCGACGGCTCGCCCTACCGCGGGGTGCT
GTTCGTCGAGCTGATGGCCACGAAGCACGGGCCCAAGCTGGTGGAGTTCAACGTCCGCTTCGGCGATCCCGAGTGCCAGG
TGCTGATGCTGCGGCTGGAGAGCGACCTCGTGCCGTACCTGGTCGCCTGCGCGAACGGGACGCTCGGGGACATGCCCCCG
CCCGTCTGGCGCGACGAGGCGGCGGTCTGCGTGGTCATCGCGGCCAAGGGCTATCCGGGCACGCCCGCCGCCGGCGGCGA
GATCCGTGGGCTCGACCAGGACCTCGGCGACGACGTGGTGGTCTTCCATGCGGGGACGAAGCGCGACGCCGACGGAACGC
TGCGGGCGGCCGGCGGCCGCGTGCTCAACGTCTGCGCGCGGGGGGCGGACCTGCGGACGGCGCGCGACCGCGCCTATGCG
GCCATCGAGCGGATCGAGTTCGCCGACGGCTTCTGCCGCAGCGACATCGGCTGGCGCGCCCTGGGCCGCTAG

Upstream 100 bases:

>100_bases
CGGGCGACCCGATCCGCACGGTCCGCTCGGCCGGCTACTCGCTGGACGTCGACGCCTGAGCGCTTGACCGGAGGGCGGCG
GACCGCCAAAGCCGCCGTCC

Downstream 100 bases:

>100_bases
CCTTCCGCCAGCGTCTTCAGGGCCGGCAGCGCCTCGTTGGCCCAGGCCTCGCCGATCGCCAGCATGGCGAGCTCGACCTC
GGCGGGCTCCAGCTCGGCCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNILLVGSGGREHALAWKIAASPLVKRLVAAPGNPGMAEQCELRAVGPTDVPALVALAKEIAADLVVIGPESSVDAGLAD
QLQAEGIPCFGPTAGAGQLESSKAFTKAFADRYGLPTAAYRVCESAEAARAALELFEPPYVIKADGLAAGKGVTVAQDRG
EAEAAIDDAFGGRFGAAGARVVVEEFLEGEIGSLFALCDGEASMLFGWAQDHKRVFDGEQGPNTGGMGTYSPAPVFTPEL
VEQVRTRLVEPAFAGIAADGSPYRGVLFVELMATKHGPKLVEFNVRFGDPECQVLMLRLESDLVPYLVACANGTLGDMPP
PVWRDEAAVCVVIAAKGYPGTPAAGGEIRGLDQDLGDDVVVFHAGTKRDADGTLRAAGGRVLNVCARGADLRTARDRAYA
AIERIEFADGFCRSDIGWRALGR

Sequences:

>Translated_423_residues
MNILLVGSGGREHALAWKIAASPLVKRLVAAPGNPGMAEQCELRAVGPTDVPALVALAKEIAADLVVIGPESSVDAGLAD
QLQAEGIPCFGPTAGAGQLESSKAFTKAFADRYGLPTAAYRVCESAEAARAALELFEPPYVIKADGLAAGKGVTVAQDRG
EAEAAIDDAFGGRFGAAGARVVVEEFLEGEIGSLFALCDGEASMLFGWAQDHKRVFDGEQGPNTGGMGTYSPAPVFTPEL
VEQVRTRLVEPAFAGIAADGSPYRGVLFVELMATKHGPKLVEFNVRFGDPECQVLMLRLESDLVPYLVACANGTLGDMPP
PVWRDEAAVCVVIAAKGYPGTPAAGGEIRGLDQDLGDDVVVFHAGTKRDADGTLRAAGGRVLNVCARGADLRTARDRAYA
AIERIEFADGFCRSDIGWRALGR
>Mature_423_residues
MNILLVGSGGREHALAWKIAASPLVKRLVAAPGNPGMAEQCELRAVGPTDVPALVALAKEIAADLVVIGPESSVDAGLAD
QLQAEGIPCFGPTAGAGQLESSKAFTKAFADRYGLPTAAYRVCESAEAARAALELFEPPYVIKADGLAAGKGVTVAQDRG
EAEAAIDDAFGGRFGAAGARVVVEEFLEGEIGSLFALCDGEASMLFGWAQDHKRVFDGEQGPNTGGMGTYSPAPVFTPEL
VEQVRTRLVEPAFAGIAADGSPYRGVLFVELMATKHGPKLVEFNVRFGDPECQVLMLRLESDLVPYLVACANGTLGDMPP
PVWRDEAAVCVVIAAKGYPGTPAAGGEIRGLDQDLGDDVVVFHAGTKRDADGTLRAAGGRVLNVCARGADLRTARDRAYA
AIERIEFADGFCRSDIGWRALGR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=43.0260047281324, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=43.0260047281324, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=43.0260047281324, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=43.0260047281324, Blast_Score=328, Evalue=5e-90,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=49.4145199063232, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=39.9527186761229, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=41.5909090909091, Blast_Score=308, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=41.9953596287703, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=41.958041958042, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_PHEZH (B4REI0)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129351.1
- GeneID:   6789999
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0508
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 44230; Mature: 44230

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLVGSGGREHALAWKIAASPLVKRLVAAPGNPGMAEQCELRAVGPTDVPALVALAKE
CEEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IAADLVVIGPESSVDAGLADQLQAEGIPCFGPTAGAGQLESSKAFTKAFADRYGLPTAAY
HHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
RVCESAEAARAALELFEPPYVIKADGLAAGKGVTVAQDRGEAEAAIDDAFGGRFGAAGAR
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCHHH
VVVEEFLEGEIGSLFALCDGEASMLFGWAQDHKRVFDGEQGPNTGGMGTYSPAPVFTPEL
HHHHHHHCCCCCCEEEEECCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
VEQVRTRLVEPAFAGIAADGSPYRGVLFVELMATKHGPKLVEFNVRFGDPECQVLMLRLE
HHHHHHHHCCHHHHCEECCCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCEEEEEEHH
SDLVPYLVACANGTLGDMPPPVWRDEAAVCVVIAAKGYPGTPAAGGEIRGLDQDLGDDVV
CCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEE
VFHAGTKRDADGTLRAAGGRVLNVCARGADLRTARDRAYAAIERIEFADGFCRSDIGWRA
EEECCCCCCCCCCEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCC
LGR
CCC
>Mature Secondary Structure
MNILLVGSGGREHALAWKIAASPLVKRLVAAPGNPGMAEQCELRAVGPTDVPALVALAKE
CEEEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IAADLVVIGPESSVDAGLADQLQAEGIPCFGPTAGAGQLESSKAFTKAFADRYGLPTAAY
HHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
RVCESAEAARAALELFEPPYVIKADGLAAGKGVTVAQDRGEAEAAIDDAFGGRFGAAGAR
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCHHH
VVVEEFLEGEIGSLFALCDGEASMLFGWAQDHKRVFDGEQGPNTGGMGTYSPAPVFTPEL
HHHHHHHCCCCCCEEEEECCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
VEQVRTRLVEPAFAGIAADGSPYRGVLFVELMATKHGPKLVEFNVRFGDPECQVLMLRLE
HHHHHHHHCCHHHHCEECCCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCEEEEEEHH
SDLVPYLVACANGTLGDMPPPVWRDEAAVCVVIAAKGYPGTPAAGGEIRGLDQDLGDDVV
CCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEE
VFHAGTKRDADGTLRAAGGRVLNVCARGADLRTARDRAYAAIERIEFADGFCRSDIGWRA
EEECCCCCCCCCCEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCC
LGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA