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The map label for this gene is nrdF [H]

Identifier: 197103810

GI number: 197103810

Start: 347088

End: 348140

Strand: Direct

Name: nrdF [H]

Synonym: PHZ_c0344

Alternate gene names: 197103810

Gene position: 347088-348140 (Clockwise)

Preceding gene: 197103808

Following gene: 197103812

Centisome position: 8.69

GC content: 64.2

Gene sequence:

>1053_bases
ATGAGCGCGAGCGCGACCTCCAACGTGAAGGCCCTGCCGGGCCTCTTGACGCCGTCCTTCGCCTACAAGCCGTTCCGGTA
TCCGTGGGCCTACGACTACTGGAAGAAGCAGCAGCAGGTTCACTGGATGCCCGAGGAGATCCCCCTCGGCGAGGACCTGA
AGGACTGGGCCTCCAAGCTCAACGACCGCGAGCGCAACCTGCTCACCCAGATCTTCCGGTTCTTCACCCAGTCGGACGTC
GAGGTGAACGACAACTACATGGAGCGCTACGCCAGAGTCTTCAAACCCACCGAGGTGAAGATGATGCTGGCGGCGTTCTC
CAACATGGAGACGATCCACATCGCCGCCTACGCCCTGCTGCTCGAGACGATCGGCATGCCCGACGCCGAGTTCACGGCCT
TCCTCGACTACCAGGCGATGCGCGACAAGCACGACTACATGCAGAGCTTCGGCGTGGACACCCACGCCGACATCTGCCGG
ACGCTGGCCATGTTCGGGGGCTTCACCGAGGGCCTGCAGCTGTTCGCCAGCTTCGCCATGCTGATGAACTTCCCGCGCCA
CAACAAGATGAAGGGCATGGGCCAGATCGTGTCGTGGTCGGTGCGGGACGAGAGCCTGCACTGCGAGGGGATCATCAAGC
TCTACCACGCCTTCAACAAGGAAACGGGCGCGGTGACCCAGGCGGTCGCCGACGACATCGTCGACTGCTGCAAGACGGTG
GTGGGCCTGGAGGACAAGTTCATCGACCTGGCCTTCGAGGCCGGCGAGGTCCAGGGCATGACCCCGGACGACATCAAGCA
GTACATCCGCTTCATCGCCGACTGGCGCCTGAAGCAGCTCCACCTGCCGCAGGTGTACGGCATCAAGGAGAACCCGCTGC
CCTGGCTGCAGTCGATGCTCTCGGGCGTCGAGCACGCCAACTTCTTCGAGGCCCGCGCGACCGAGTACTCCAAGGCCGCG
ACCAAGGGCCAATGGCACGGCGAGGCCGGCGTCTGGGCCGAATTCGACCGGATGCAGGCCAAGCGCGCCGCGAACGAGAT
CCCGGCGGAGTAG

Upstream 100 bases:

>100_bases
CCTCGTGGAAAACTCTAGATGCGGCGTACCGGCCCCTTTACGCCCACAAGATGTTGCGTCACCTTTGGCTGACGCGAGAG
CCAGGGGCAGGATGAACGAG

Downstream 100 bases:

>100_bases
GCGGCGAGCCGTCCCGGGGCGGCGCATCCATCTTTCCCGCCGTCATCCCGGAAAGCCGCGGCGCGGCTTGTTCGGACCCG
TCCGCGCCGGGAGGCGCCGG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSASATSNVKALPGLLTPSFAYKPFRYPWAYDYWKKQQQVHWMPEEIPLGEDLKDWASKLNDRERNLLTQIFRFFTQSDV
EVNDNYMERYARVFKPTEVKMMLAAFSNMETIHIAAYALLLETIGMPDAEFTAFLDYQAMRDKHDYMQSFGVDTHADICR
TLAMFGGFTEGLQLFASFAMLMNFPRHNKMKGMGQIVSWSVRDESLHCEGIIKLYHAFNKETGAVTQAVADDIVDCCKTV
VGLEDKFIDLAFEAGEVQGMTPDDIKQYIRFIADWRLKQLHLPQVYGIKENPLPWLQSMLSGVEHANFFEARATEYSKAA
TKGQWHGEAGVWAEFDRMQAKRAANEIPAE

Sequences:

>Translated_350_residues
MSASATSNVKALPGLLTPSFAYKPFRYPWAYDYWKKQQQVHWMPEEIPLGEDLKDWASKLNDRERNLLTQIFRFFTQSDV
EVNDNYMERYARVFKPTEVKMMLAAFSNMETIHIAAYALLLETIGMPDAEFTAFLDYQAMRDKHDYMQSFGVDTHADICR
TLAMFGGFTEGLQLFASFAMLMNFPRHNKMKGMGQIVSWSVRDESLHCEGIIKLYHAFNKETGAVTQAVADDIVDCCKTV
VGLEDKFIDLAFEAGEVQGMTPDDIKQYIRFIADWRLKQLHLPQVYGIKENPLPWLQSMLSGVEHANFFEARATEYSKAA
TKGQWHGEAGVWAEFDRMQAKRAANEIPAE
>Mature_349_residues
SASATSNVKALPGLLTPSFAYKPFRYPWAYDYWKKQQQVHWMPEEIPLGEDLKDWASKLNDRERNLLTQIFRFFTQSDVE
VNDNYMERYARVFKPTEVKMMLAAFSNMETIHIAAYALLLETIGMPDAEFTAFLDYQAMRDKHDYMQSFGVDTHADICRT
LAMFGGFTEGLQLFASFAMLMNFPRHNKMKGMGQIVSWSVRDESLHCEGIIKLYHAFNKETGAVTQAVADDIVDCCKTVV
GLEDKFIDLAFEAGEVQGMTPDDIKQYIRFIADWRLKQLHLPQVYGIKENPLPWLQSMLSGVEHANFFEARATEYSKAAT
KGQWHGEAGVWAEFDRMQAKRAANEIPAE

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=324, Percent_Identity=28.7037037037037, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI289177074, Length=324, Percent_Identity=28.7037037037037, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI260064013, Length=305, Percent_Identity=28.5245901639344, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI4557845, Length=305, Percent_Identity=28.5245901639344, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI289177076, Length=286, Percent_Identity=28.6713286713287, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788567, Length=300, Percent_Identity=20, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17551968, Length=307, Percent_Identity=28.9902280130293, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193206379, Length=285, Percent_Identity=24.5614035087719, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322434, Length=304, Percent_Identity=27.3026315789474, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18079279, Length=303, Percent_Identity=28.3828382838284, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40158; Mature: 40027

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASATSNVKALPGLLTPSFAYKPFRYPWAYDYWKKQQQVHWMPEEIPLGEDLKDWASKL
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
NDRERNLLTQIFRFFTQSDVEVNDNYMERYARVFKPTEVKMMLAAFSNMETIHIAAYALL
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHH
LETIGMPDAEFTAFLDYQAMRDKHDYMQSFGVDTHADICRTLAMFGGFTEGLQLFASFAM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
LMNFPRHNKMKGMGQIVSWSVRDESLHCEGIIKLYHAFNKETGAVTQAVADDIVDCCKTV
HHCCCCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VGLEDKFIDLAFEAGEVQGMTPDDIKQYIRFIADWRLKQLHLPQVYGIKENPLPWLQSML
HCCCHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHH
SGVEHANFFEARATEYSKAATKGQWHGEAGVWAEFDRMQAKRAANEIPAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SASATSNVKALPGLLTPSFAYKPFRYPWAYDYWKKQQQVHWMPEEIPLGEDLKDWASKL
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH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HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]