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The map label for this gene is kefB [H]

Identifier: 197103802

GI number: 197103802

Start: 333908

End: 335707

Strand: Direct

Name: kefB [H]

Synonym: PHZ_c0336

Alternate gene names: 197103802

Gene position: 333908-335707 (Clockwise)

Preceding gene: 197103800

Following gene: 197103803

Centisome position: 8.36

GC content: 73.83

Gene sequence:

>1800_bases
ATGGAAGGCCACGTCGAGACCGGCGCGTACAAGGACGTGGTCCTGTTCCTGGCCACCGCCGGCGTCGTGGTCCCGCTGTT
CCGCCGCTGGCGGATCAGCCCGATCCTGGGCTTCCTCGTGGCCGGCGTCCTCCTGGGCCCGTTCGGCCTGGGCAGCCTCT
CGCACCAGTTCGGCTGGCTGCGCGCCATGACCATCGACGAGCCCGAGCAGCTCTCGGTGCTGGCCGAGCTGGGCGTCGTC
TTCCTGCTGTTCATGATCGGGCTGGAGCTGTCGTGGGAGCGCCTGCGCGCGATGCGCCGCTACGTCTTCGGGCTGGGGGC
GCTGCAGGTGGGCCTGTGCCTGGCCGGCGTCGCCGGCGCGGCCCTGCTGCTGGGCCAGCGGCCGCTGGCGGCCCTGGCGA
TCGGCGCGGCCCTGGCGCTGTCCTCTACCGCCATCGTCATGCCGATCCTGGCCGAGCAGAAGCGCCAGCATTCGCAGGCC
GGCCGCGCCACCTTCTCGGTCCTGCTGTTCCAGGACCTGGCCGTCGCCCCGATCCTCGTCACCCTCACCCTGCTCAGCCG
GCAGGAGGACGCCGTCTTCGGCCCCGCGCTGCTGCTCTCGTTCGCCCCGGCGGTGCTTGGCCTGGCCGCCCTGTTCCTGA
TGGGCCGGCTGCTGTTGCGGCCGATGCTGCGCTCGGTGGCCAAGGCCAAGAGCGAGGAGCTGTTCGTCGCCGCCAGCCTC
CTAGTGGTCATCGGCGCCGGCCTGGTCAGCGCGCTGACCGGCCTGTCCATGGCGCTCGGCGCGTTCATCGCCGGCCTGCT
GCTGGCCGAGACCGAGTTCCGCCACGAGGTCGAGGTGCGGGTGGAGCCCTTCAAGGGCCTGCTGCTCGGCCTGTTCTTCC
TGTCGGTGGGCATCGGCCTCAACGTGCCGCTGCTGGTGGAGCGGCCGGCGGCCATCCTCGGCATCGCCCTGGCGATGGTG
GCCGGCAACGCCCTGGTGGTCTTCGGCCTCGCGCGGCTGTTCGGCCTGCGCTCGCGGCCCGCCGCGGAGGCCGCCCTTCT
GCTCGCCGGCGCGGGCGAGTTCGCCTTCGTCATCCTGGGCCAGGCCATGGGCGGCGGCATCGTCGACCGCACGACCGGCG
AGACGGTGCTGGTCGCCGCGACCGTCTCGATGTTCTGCATCCCGCTGCTCTCCCTGCTCAGCGTCCGGGTCGGAGGGCGC
AAGGTCGCCCCCGCCGAGACCCCGGCGGAACCCGAGGGGGCCGAGCCCGGGGGAACCGAGCCTGGGGGGACGCCGCGCGT
GCTGGTGGTCGGCTACGGCCGGGTCGGCCGCCTCGTCGGCGACATGCTGAACCGGCATCAGGTCGCCTGGGTGGGGGCCG
AGAAGGACGCCCGCATGGTCGACACCGGCCGCCGCGCGGGCCAGCGGATCTACTTCGGCGATGCGGCCCGGCCCGAGTTC
CTCGAGCGCTGCGGTCTGGCCGAGGCCCCGGCGCTCGTGGTGACCATGGACGATCCCGAGGGGGTGGAGGCGATCGTCGG
CCTCGCGCGTTCCCTCCGTCCGGACCTGACGATCGTCGCCCGCGCCCGCGACGCCCGCCATGCCCAGCGGCTCTACGAGC
TCGGCGCCACCGACGCCGTGCCCGAGACGGTGGAGGCCAGCCTGCAGCTCTCCGAGGCGGTGCTGGTAGAGATCGGCGTG
CCGATGGGCCTGGTGATCGCCTCGATCCACGAGCGGCGCGACGAGTTCCGGGCGGCGCTCAACCGGCCCGAGGCGCTCGG
CGGCCGCGCCCGCCGCTTCCGCCGCGAGCGCGGCGCCTGA

Upstream 100 bases:

>100_bases
GTCCCGCGGCGTGGTCACGGAACGCCGTCGTAAGGCCGTGGCGGGATTACGCAAGCCCGCCCGCTGCGTCATAGTCCGCG
GGAATCAACCCGGAGAGCCG

Downstream 100 bases:

>100_bases
CCGGCGCCGGGAATTTGGCGGCGGAAATATTGTCAGCTTGCGTTCATGTGTCGGGAACCAAGCGTTGCCATGCACGTTGA
GGCCCCGAGCGCGAAAGGGG

Product: kef-type K+ transport system, membrane component

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MEGHVETGAYKDVVLFLATAGVVVPLFRRWRISPILGFLVAGVLLGPFGLGSLSHQFGWLRAMTIDEPEQLSVLAELGVV
FLLFMIGLELSWERLRAMRRYVFGLGALQVGLCLAGVAGAALLLGQRPLAALAIGAALALSSTAIVMPILAEQKRQHSQA
GRATFSVLLFQDLAVAPILVTLTLLSRQEDAVFGPALLLSFAPAVLGLAALFLMGRLLLRPMLRSVAKAKSEELFVAASL
LVVIGAGLVSALTGLSMALGAFIAGLLLAETEFRHEVEVRVEPFKGLLLGLFFLSVGIGLNVPLLVERPAAILGIALAMV
AGNALVVFGLARLFGLRSRPAAEAALLLAGAGEFAFVILGQAMGGGIVDRTTGETVLVAATVSMFCIPLLSLLSVRVGGR
KVAPAETPAEPEGAEPGGTEPGGTPRVLVVGYGRVGRLVGDMLNRHQVAWVGAEKDARMVDTGRRAGQRIYFGDAARPEF
LERCGLAEAPALVVTMDDPEGVEAIVGLARSLRPDLTIVARARDARHAQRLYELGATDAVPETVEASLQLSEAVLVEIGV
PMGLVIASIHERRDEFRAALNRPEALGGRARRFRRERGA

Sequences:

>Translated_599_residues
MEGHVETGAYKDVVLFLATAGVVVPLFRRWRISPILGFLVAGVLLGPFGLGSLSHQFGWLRAMTIDEPEQLSVLAELGVV
FLLFMIGLELSWERLRAMRRYVFGLGALQVGLCLAGVAGAALLLGQRPLAALAIGAALALSSTAIVMPILAEQKRQHSQA
GRATFSVLLFQDLAVAPILVTLTLLSRQEDAVFGPALLLSFAPAVLGLAALFLMGRLLLRPMLRSVAKAKSEELFVAASL
LVVIGAGLVSALTGLSMALGAFIAGLLLAETEFRHEVEVRVEPFKGLLLGLFFLSVGIGLNVPLLVERPAAILGIALAMV
AGNALVVFGLARLFGLRSRPAAEAALLLAGAGEFAFVILGQAMGGGIVDRTTGETVLVAATVSMFCIPLLSLLSVRVGGR
KVAPAETPAEPEGAEPGGTEPGGTPRVLVVGYGRVGRLVGDMLNRHQVAWVGAEKDARMVDTGRRAGQRIYFGDAARPEF
LERCGLAEAPALVVTMDDPEGVEAIVGLARSLRPDLTIVARARDARHAQRLYELGATDAVPETVEASLQLSEAVLVEIGV
PMGLVIASIHERRDEFRAALNRPEALGGRARRFRRERGA
>Mature_599_residues
MEGHVETGAYKDVVLFLATAGVVVPLFRRWRISPILGFLVAGVLLGPFGLGSLSHQFGWLRAMTIDEPEQLSVLAELGVV
FLLFMIGLELSWERLRAMRRYVFGLGALQVGLCLAGVAGAALLLGQRPLAALAIGAALALSSTAIVMPILAEQKRQHSQA
GRATFSVLLFQDLAVAPILVTLTLLSRQEDAVFGPALLLSFAPAVLGLAALFLMGRLLLRPMLRSVAKAKSEELFVAASL
LVVIGAGLVSALTGLSMALGAFIAGLLLAETEFRHEVEVRVEPFKGLLLGLFFLSVGIGLNVPLLVERPAAILGIALAMV
AGNALVVFGLARLFGLRSRPAAEAALLLAGAGEFAFVILGQAMGGGIVDRTTGETVLVAATVSMFCIPLLSLLSVRVGGR
KVAPAETPAEPEGAEPGGTEPGGTPRVLVVGYGRVGRLVGDMLNRHQVAWVGAEKDARMVDTGRRAGQRIYFGDAARPEF
LERCGLAEAPALVVTMDDPEGVEAIVGLARSLRPDLTIVARARDARHAQRLYELGATDAVPETVEASLQLSEAVLVEIGV
PMGLVIASIHERRDEFRAALNRPEALGGRARRFRRERGA

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=364, Percent_Identity=27.1978021978022, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789749, Length=589, Percent_Identity=38.3701188455008, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI1786232, Length=565, Percent_Identity=35.3982300884956, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1786685, Length=525, Percent_Identity=31.6190476190476, Blast_Score=171, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6322367, Length=422, Percent_Identity=24.6445497630332, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 63310; Mature: 63310

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGHVETGAYKDVVLFLATAGVVVPLFRRWRISPILGFLVAGVLLGPFGLGSLSHQFGWL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCE
RAMTIDEPEQLSVLAELGVVFLLFMIGLELSWERLRAMRRYVFGLGALQVGLCLAGVAGA
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLLGQRPLAALAIGAALALSSTAIVMPILAEQKRQHSQAGRATFSVLLFQDLAVAPILV
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLTLLSRQEDAVFGPALLLSFAPAVLGLAALFLMGRLLLRPMLRSVAKAKSEELFVAASL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LVVIGAGLVSALTGLSMALGAFIAGLLLAETEFRHEVEVRVEPFKGLLLGLFFLSVGIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCC
NVPLLVERPAAILGIALAMVAGNALVVFGLARLFGLRSRPAAEAALLLAGAGEFAFVILG
CCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHEECCCCEEEEEEE
QAMGGGIVDRTTGETVLVAATVSMFCIPLLSLLSVRVGGRKVAPAETPAEPEGAEPGGTE
HHHCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCC
PGGTPRVLVVGYGRVGRLVGDMLNRHQVAWVGAEKDARMVDTGRRAGQRIYFGDAARPEF
CCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCEEEECCCCCHHH
LERCGLAEAPALVVTMDDPEGVEAIVGLARSLRPDLTIVARARDARHAQRLYELGATDAV
HHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCC
PETVEASLQLSEAVLVEIGVPMGLVIASIHERRDEFRAALNRPEALGGRARRFRRERGA
CHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MEGHVETGAYKDVVLFLATAGVVVPLFRRWRISPILGFLVAGVLLGPFGLGSLSHQFGWL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCE
RAMTIDEPEQLSVLAELGVVFLLFMIGLELSWERLRAMRRYVFGLGALQVGLCLAGVAGA
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLLGQRPLAALAIGAALALSSTAIVMPILAEQKRQHSQAGRATFSVLLFQDLAVAPILV
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLTLLSRQEDAVFGPALLLSFAPAVLGLAALFLMGRLLLRPMLRSVAKAKSEELFVAASL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LVVIGAGLVSALTGLSMALGAFIAGLLLAETEFRHEVEVRVEPFKGLLLGLFFLSVGIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCC
NVPLLVERPAAILGIALAMVAGNALVVFGLARLFGLRSRPAAEAALLLAGAGEFAFVILG
CCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHEECCCCEEEEEEE
QAMGGGIVDRTTGETVLVAATVSMFCIPLLSLLSVRVGGRKVAPAETPAEPEGAEPGGTE
HHHCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCC
PGGTPRVLVVGYGRVGRLVGDMLNRHQVAWVGAEKDARMVDTGRRAGQRIYFGDAARPEF
CCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCEEEECCCCCHHH
LERCGLAEAPALVVTMDDPEGVEAIVGLARSLRPDLTIVARARDARHAQRLYELGATDAV
HHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCC
PETVEASLQLSEAVLVEIGVPMGLVIASIHERRDEFRAALNRPEALGGRARRFRRERGA
CHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA