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The map label for this gene is 197103791

Identifier: 197103791

GI number: 197103791

Start: 319983

End: 320861

Strand: Direct

Name: 197103791

Synonym: PHZ_c0325

Alternate gene names: NA

Gene position: 319983-320861 (Clockwise)

Preceding gene: 197103790

Following gene: 197103794

Centisome position: 8.01

GC content: 69.74

Gene sequence:

>879_bases
ATGACGCGCAAGCTCTCCGTCGCCGCCATCCAGACCTCGTACGGCCAGGACATGGCGGCCAACATCGCCAAGACCGAAGG
CTTCATCCGCCAGGCCGCGGCCGACGGCGCCCAGGTGATCCTCCCCTCCGAGCTGTTCCAGGGGCCCTACTTCTGCGTGG
CCCAGGAGGAGCGCTGGTTCGCCACCGCCTATCCCTGGCGCGAGCATCCGTGCGTCACCGCGCTGGCGCCGCTGGCCAAG
GAGCTGGGCGTCGTGCTGCCGATCTCGATCTTCGAGCGCGAGGGGCCGCACTACTTCAACAGCCTGGTGATGGTGGACGC
CGACGGCTCGCTGATGGGCGTCTATCGCAAGAGCCACATCCCCGACGGACCGGGCTACATGGAGAAGTACTACTTCCGGC
CCGGCGACACCGGCTTCAAGGTCTGGGAGACGAAGTTCGGGCGCATCGGCGTCGGCATCTGCTGGGACCAGTGGTACCCC
GAGGCCGCCCGCGCCATGACCCTGATGGGCGCCGAGGTCTTGCTCTATCCCACCGCCATCGGCTCCGAGCCGCACGACGC
CACCCTGGACACCGCCGCCCCCTGGCGCCGGGCGATGCAGGGCCACGCGGTCTCGAACGTGATCCCGGTGGTGGGCGCCA
ACCGCACGGGCTTCGAGCCGTGGGACGGCTATCCGAACGGCGGCCAGGAGTTCTACGGCTCCAGCTTCATCGCCGACCAC
CGGGGCGACCTCGTCGCCGCCTTCGGGCGCGAGGACGAGGGGGTGCTGAAGGCCGAGTTCGACCTCGACTTCCTGGCGAC
CCACCGCGCCGCCTGGGGCTTCTTCCGCGACCGCCGCACCGACCTCTATGGGTCGCTGGTGAACGGCCGGCCCGCGTAA

Upstream 100 bases:

>100_bases
AGCTGTTCCCCGGCCGCGCGATCGTCGGCCTTCCGTCGCTCGCCATTCTGACCGGCGGCGGGTCCTTCCACTGCATCACC
CAGCAGGAGCCCGCCCTCTG

Downstream 100 bases:

>100_bases
GTCGTCTCCTCTCCCCTCCGACTACGCCTCGGGGGAGAGGAGAGGGGGATCAGCAGCCCGTCGCGGCGGGCGCGCCGGCC
AGTTCCTGCTTGGCCTCCGC

Product: hydrolase, carbon-nitrogen family

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTRKLSVAAIQTSYGQDMAANIAKTEGFIRQAAADGAQVILPSELFQGPYFCVAQEERWFATAYPWREHPCVTALAPLAK
ELGVVLPISIFEREGPHYFNSLVMVDADGSLMGVYRKSHIPDGPGYMEKYYFRPGDTGFKVWETKFGRIGVGICWDQWYP
EAARAMTLMGAEVLLYPTAIGSEPHDATLDTAAPWRRAMQGHAVSNVIPVVGANRTGFEPWDGYPNGGQEFYGSSFIADH
RGDLVAAFGREDEGVLKAEFDLDFLATHRAAWGFFRDRRTDLYGSLVNGRPA

Sequences:

>Translated_292_residues
MTRKLSVAAIQTSYGQDMAANIAKTEGFIRQAAADGAQVILPSELFQGPYFCVAQEERWFATAYPWREHPCVTALAPLAK
ELGVVLPISIFEREGPHYFNSLVMVDADGSLMGVYRKSHIPDGPGYMEKYYFRPGDTGFKVWETKFGRIGVGICWDQWYP
EAARAMTLMGAEVLLYPTAIGSEPHDATLDTAAPWRRAMQGHAVSNVIPVVGANRTGFEPWDGYPNGGQEFYGSSFIADH
RGDLVAAFGREDEGVLKAEFDLDFLATHRAAWGFFRDRRTDLYGSLVNGRPA
>Mature_291_residues
TRKLSVAAIQTSYGQDMAANIAKTEGFIRQAAADGAQVILPSELFQGPYFCVAQEERWFATAYPWREHPCVTALAPLAKE
LGVVLPISIFEREGPHYFNSLVMVDADGSLMGVYRKSHIPDGPGYMEKYYFRPGDTGFKVWETKFGRIGVGICWDQWYPE
AARAMTLMGAEVLLYPTAIGSEPHDATLDTAAPWRRAMQGHAVSNVIPVVGANRTGFEPWDGYPNGGQEFYGSSFIADHR
GDLVAAFGREDEGVLKAEFDLDFLATHRAAWGFFRDRRTDLYGSLVNGRPA

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=284, Percent_Identity=26.4084507042254, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI9910460, Length=290, Percent_Identity=28.2758620689655, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI5031947, Length=291, Percent_Identity=28.1786941580756, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI297632350, Length=291, Percent_Identity=28.1786941580756, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI297632348, Length=291, Percent_Identity=28.1786941580756, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI297632346, Length=187, Percent_Identity=28.3422459893048, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17533173, Length=309, Percent_Identity=31.7152103559871, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17556280, Length=299, Percent_Identity=25.752508361204, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=298, Percent_Identity=28.1879194630872, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322335, Length=318, Percent_Identity=25.1572327044025, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358471, Length=306, Percent_Identity=30.718954248366, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17933642, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32361; Mature: 32230

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKLSVAAIQTSYGQDMAANIAKTEGFIRQAAADGAQVILPSELFQGPYFCVAQEERWF
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCEEEEECCCCEE
ATAYPWREHPCVTALAPLAKELGVVLPISIFEREGPHYFNSLVMVDADGSLMGVYRKSHI
EEECCCCCCCHHHHHHHHHHHCCEEEEEHHHCCCCCCHHCEEEEEECCCCEEEEEHHCCC
PDGPGYMEKYYFRPGDTGFKVWETKFGRIGVGICWDQWYPEAARAMTLMGAEVLLYPTAI
CCCCCHHHHEEECCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECC
GSEPHDATLDTAAPWRRAMQGHAVSNVIPVVGANRTGFEPWDGYPNGGQEFYGSSFIADH
CCCCCCCCCCCHHHHHHHHCCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHCCCCEEECC
RGDLVAAFGREDEGVLKAEFDLDFLATHRAAWGFFRDRRTDLYGSLVNGRPA
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRKLSVAAIQTSYGQDMAANIAKTEGFIRQAAADGAQVILPSELFQGPYFCVAQEERWF
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCEEEEECCCCEE
ATAYPWREHPCVTALAPLAKELGVVLPISIFEREGPHYFNSLVMVDADGSLMGVYRKSHI
EEECCCCCCCHHHHHHHHHHHCCEEEEEHHHCCCCCCHHCEEEEEECCCCEEEEEHHCCC
PDGPGYMEKYYFRPGDTGFKVWETKFGRIGVGICWDQWYPEAARAMTLMGAEVLLYPTAI
CCCCCHHHHEEECCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECC
GSEPHDATLDTAAPWRRAMQGHAVSNVIPVVGANRTGFEPWDGYPNGGQEFYGSSFIADH
CCCCCCCCCCCHHHHHHHHCCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHCCCCEEECC
RGDLVAAFGREDEGVLKAEFDLDFLATHRAAWGFFRDRRTDLYGSLVNGRPA
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]