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The map label for this gene is phaZ

Identifier: 197103695

GI number: 197103695

Start: 230508

End: 231812

Strand: Direct

Name: phaZ

Synonym: PHZ_c0229

Alternate gene names: NA

Gene position: 230508-231812 (Clockwise)

Preceding gene: 197103694

Following gene: 197103696

Centisome position: 5.77

GC content: 69.58

Gene sequence:

>1305_bases
ATGTTATGCTGCGGCGCACAAAAAGGGGGCCTGATGCTCTACACCCTCTATGAGGCCGGCTACTACGCCTCGACCCCGCT
CCGTCTGGCCGCCCTGGCGACCCGGCAGTTCTGGGGCTCGCCGCTCAATCCGGCCGCCGACACCGAGCTGGGCCGGCGGC
TGTTCGCCACCTCGGACCTGATCACCAACCTGACCCGGCGCTACGGCAAGCCGGCGTGGGGGATCGACCAGGTCGAGATC
GGCGGGCAGGACGTGCGGGTGCGGCCGGTCAGCGTCTGGTCCAGCCCCTGGGCGAAGCTGACCCACTTCGTGCGCGACAT
GTCCGACCTGCGGCGGGCGGGCCGGCGCGAGCTGGAGCCGGCGGTGCTGATCGTGGCGCCGCTCTCGGGGCACTACGCCA
CCCTGCTGCGCGGCACGGTGCAGGCCTTCCTGCAGGACCACGAGGTGTTCGTCACCGAATGGGCGAACGCCCGCGACGTG
CCGGTGCTGGAAGGCCGCTTCGACCTCGACGACTACATCGACCACATCCGCGAGATGCTGCGCGTGCTGGGCCCGCGCCC
GCACGTGGTGGCGGTCTGCCAGCCGGGGCCCGCGGTCCTGGCGGCCGCCGCGCTGATGAGCGAGGACGGCGAGGAGAGCC
GCCCGGGCTCGATGACCTTCATGGGCTCGCCGATCGACGCGCGGCTGTCGCCCACGGTGACCAACCGGCTGGCCGAGGAG
CGGCCGTTCGCCTGGTTCGAGAGCAACATGGTCTACACCGTCCCCGGGCCCTATCCGGGGATGGGGCGGCGGGTCTATCC
GGGCTTCGTCCAGCTCGCGAGCTTCATGTCGATGAACCTCGAGAAGCACCAGGAAGCCCACCTGCGCTACCTGCAGCACC
TGATGGACGGCGACGGGGATTCCGCCGACAAGCACCTGGAGTTCTACGACGAGTACCTCTCGGTGCTGGACCTGACCGAG
GAGTTCTACCTCCAGACCGTCGACGTGGTGTTCCAGCGCTACCTGCTGCCGAAGGGCGAGTTCTTCCACCGCGGCCGCCA
CGTGCGGCCCGACCGGATCGCCGACATCGGCCTGATGACCGTCGAGGGCGAGAACGACGACATCTCCGGGATCGGCCAGA
CCCAGGCGGCCCACGACCTGTGCTCGGGCATTCCGGCCGAGCTGAAGGAGGACTGGGTGCAGCCGCACGTCGGCCACTAC
GGCGTCTTCAACGGCAAGCGCTTCCGCGAGGAGATCTACCCGCGGGTGAGGGCGTTCATCCGCCGCATCGAAGAGGGCTT
CGACAGGACCCGGGCGGCTGCCTAG

Upstream 100 bases:

>100_bases
TCCCACCATTCGCTCTTCAGCCCGCCGACGGGAGCTTCGGCGAATGGTCCCCCCTTCCCGCTTCGCGGGACGGGCAGGGT
GGAGCCAATTAACGCTTCAC

Downstream 100 bases:

>100_bases
ATAAGGGGCGATGAGTCCCTTCGGCCGAGCCTTCACCGACGGAGACCGGCTGGAGGTGGCCGGGGCGGTCGTGCGCCTGC
GCGTGAACCGCCGCGCCCGC

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLCCGAQKGGLMLYTLYEAGYYASTPLRLAALATRQFWGSPLNPAADTELGRRLFATSDLITNLTRRYGKPAWGIDQVEI
GGQDVRVRPVSVWSSPWAKLTHFVRDMSDLRRAGRRELEPAVLIVAPLSGHYATLLRGTVQAFLQDHEVFVTEWANARDV
PVLEGRFDLDDYIDHIREMLRVLGPRPHVVAVCQPGPAVLAAAALMSEDGEESRPGSMTFMGSPIDARLSPTVTNRLAEE
RPFAWFESNMVYTVPGPYPGMGRRVYPGFVQLASFMSMNLEKHQEAHLRYLQHLMDGDGDSADKHLEFYDEYLSVLDLTE
EFYLQTVDVVFQRYLLPKGEFFHRGRHVRPDRIADIGLMTVEGENDDISGIGQTQAAHDLCSGIPAELKEDWVQPHVGHY
GVFNGKRFREEIYPRVRAFIRRIEEGFDRTRAAA

Sequences:

>Translated_434_residues
MLCCGAQKGGLMLYTLYEAGYYASTPLRLAALATRQFWGSPLNPAADTELGRRLFATSDLITNLTRRYGKPAWGIDQVEI
GGQDVRVRPVSVWSSPWAKLTHFVRDMSDLRRAGRRELEPAVLIVAPLSGHYATLLRGTVQAFLQDHEVFVTEWANARDV
PVLEGRFDLDDYIDHIREMLRVLGPRPHVVAVCQPGPAVLAAAALMSEDGEESRPGSMTFMGSPIDARLSPTVTNRLAEE
RPFAWFESNMVYTVPGPYPGMGRRVYPGFVQLASFMSMNLEKHQEAHLRYLQHLMDGDGDSADKHLEFYDEYLSVLDLTE
EFYLQTVDVVFQRYLLPKGEFFHRGRHVRPDRIADIGLMTVEGENDDISGIGQTQAAHDLCSGIPAELKEDWVQPHVGHY
GVFNGKRFREEIYPRVRAFIRRIEEGFDRTRAAA
>Mature_434_residues
MLCCGAQKGGLMLYTLYEAGYYASTPLRLAALATRQFWGSPLNPAADTELGRRLFATSDLITNLTRRYGKPAWGIDQVEI
GGQDVRVRPVSVWSSPWAKLTHFVRDMSDLRRAGRRELEPAVLIVAPLSGHYATLLRGTVQAFLQDHEVFVTEWANARDV
PVLEGRFDLDDYIDHIREMLRVLGPRPHVVAVCQPGPAVLAAAALMSEDGEESRPGSMTFMGSPIDARLSPTVTNRLAEE
RPFAWFESNMVYTVPGPYPGMGRRVYPGFVQLASFMSMNLEKHQEAHLRYLQHLMDGDGDSADKHLEFYDEYLSVLDLTE
EFYLQTVDVVFQRYLLPKGEFFHRGRHVRPDRIADIGLMTVEGENDDISGIGQTQAAHDLCSGIPAELKEDWVQPHVGHY
GVFNGKRFREEIYPRVRAFIRRIEEGFDRTRAAA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 49003; Mature: 49003

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCCGAQKGGLMLYTLYEAGYYASTPLRLAALATRQFWGSPLNPAADTELGRRLFATSDL
CEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ITNLTRRYGKPAWGIDQVEIGGQDVRVRPVSVWSSPWAKLTHFVRDMSDLRRAGRRELEP
HHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
AVLIVAPLSGHYATLLRGTVQAFLQDHEVFVTEWANARDVPVLEGRFDLDDYIDHIREML
EEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHHHH
RVLGPRPHVVAVCQPGPAVLAAAALMSEDGEESRPGSMTFMGSPIDARLSPTVTNRLAEE
HHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
RPFAWFESNMVYTVPGPYPGMGRRVYPGFVQLASFMSMNLEKHQEAHLRYLQHLMDGDGD
CCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SADKHLEFYDEYLSVLDLTEEFYLQTVDVVFQRYLLPKGEFFHRGRHVRPDRIADIGLMT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHCCEEEE
VEGENDDISGIGQTQAAHDLCSGIPAELKEDWVQPHVGHYGVFNGKRFREEIYPRVRAFI
EECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RRIEEGFDRTRAAA
HHHHHHHHHHHCCC
>Mature Secondary Structure
MLCCGAQKGGLMLYTLYEAGYYASTPLRLAALATRQFWGSPLNPAADTELGRRLFATSDL
CEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ITNLTRRYGKPAWGIDQVEIGGQDVRVRPVSVWSSPWAKLTHFVRDMSDLRRAGRRELEP
HHHHHHHHCCCCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
AVLIVAPLSGHYATLLRGTVQAFLQDHEVFVTEWANARDVPVLEGRFDLDDYIDHIREML
EEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEECCCCCHHHHHHHHHHHH
RVLGPRPHVVAVCQPGPAVLAAAALMSEDGEESRPGSMTFMGSPIDARLSPTVTNRLAEE
HHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
RPFAWFESNMVYTVPGPYPGMGRRVYPGFVQLASFMSMNLEKHQEAHLRYLQHLMDGDGD
CCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SADKHLEFYDEYLSVLDLTEEFYLQTVDVVFQRYLLPKGEFFHRGRHVRPDRIADIGLMT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHCCEEEE
VEGENDDISGIGQTQAAHDLCSGIPAELKEDWVQPHVGHYGVFNGKRFREEIYPRVRAFI
EECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RRIEEGFDRTRAAA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA