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The map label for this gene is thcD [H]

Identifier: 197103680

GI number: 197103680

Start: 211744

End: 212973

Strand: Direct

Name: thcD [H]

Synonym: PHZ_c0214

Alternate gene names: 197103680

Gene position: 211744-212973 (Clockwise)

Preceding gene: 197103679

Following gene: 197103681

Centisome position: 5.3

GC content: 72.76

Gene sequence:

>1230_bases
ATGAGCGACGCTACGGCGCATGTGGTGATCCTGGGCGCGGGCCATGCCGGCGGCACGGCCGCCGCGCTGCTGCGCCAGTA
CGGCCACGAGGGCCCCATCACCCTGGTGGGCGAGGAGCCGATCCCGCCCTACCAGCGCCCGCCGCTCTCCAAGGCCTGGC
TGAAGGGCGAGGCCGACGCCGACTCCCTGGCCCTCAAGCCCCTGGAGTTCTACGCCGAGCACGGAATCGACTTCCGCCCG
GGCGTGAAGGCGGTGCAGCTCAAGCGCAGCGACCGCACCGTCGTCCTCTCCGACGGCTCGACCCTCGCCTACGACGTCCT
GATCATCGCCACCGGCGCCCGGCCCATCGCCCTGCCGATCCCCGGCGCCGACCTGGCCGGCGTCATGTTCCTGCGCACCG
CCGCCGACGCCGAACAGCTCAAGGCCGCCGTCGGACCGGGCAAGCGCCTGGCCGTGGTGGGCGGCGGCTACATCGGGCTG
GAGGTCGCCGCCTCGGGCAGGGCCCTGGGCGCCGAGGTCACCGTGCTGGAGCGCGAGCCGCGCCTGCTGGCCCGGGTCGC
CTGCCCCGAGCTCTCCACCTTCTTCCGCGAATACCACGAGAAGCACGGCGTCACGTTCGAGCTGGGCTGCAGCGTCACCG
GCTTCGAGGGCGAGGCCGGCCGCGTCACCGGCGTCACCCTGGCCGACGGCCGCACGATCGCCTGCGACGCCGCGGTGGTC
GGCGTGGGCGCGGCCCCCAACGACGAGATCGCCGCCGACGCGGGCCTCGAGACCGCCCGCGGCGTGGTGGTCGACCTCGA
CGCGCGGACCTCGGACCCGGCCGTCTTCGCCATCGGCGACGTCGCCCACCGGCCGATGCCGATCTACGACCGGATGTTCC
GGATGGAGAGCGTGCCCAACGCCCTGGAGCAGGCCAAGCAGGCCGCCGCCGCCATCACCGGCCGCCCCCGCCCGCCGGGC
GAGTGCCCCTGGCAGTGGTCGGACCAGTACGACCTGAAGCTGCAGATCGCCGGCTACCCCTTCGACGCCGACCAGATCGT
GGTGCGGGGCGACCCGGCCACGGCGAAGTTCGCCGTCTTCCACCTGAAGGGGAGCCAGGTGCAGGCCGTCGAGGCGATCA
ACGCCCCGCCCGAATTCATGATGGGCAAGCAGCTCATCGCGAACCGCAAGCCGGTGGACCCCGCAAGGCTTGCCGACCCC
ACGATTTCCATGAAAGAGGTCGCCGCTTAA

Upstream 100 bases:

>100_bases
TCAAGAAGCTGCTGGTGGACCGGGTCGTCTGACGCCCGGGGCCGCCGGGTTCCGGCGGGGAAAAGTTGACGGGGCCGTCA
CTTTTGGGCATCGAGGTTCC

Downstream 100 bases:

>100_bases
GCGAGCGGCTCACGAGGAGCGCCATGGCCAAGATCACCTACATCGAGCACGACGGCACCGAACACGTCATCGACGTGAAG
CCCGGGCTCTCGGTCATGGA

Product: ferredoxin reductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSDATAHVVILGAGHAGGTAAALLRQYGHEGPITLVGEEPIPPYQRPPLSKAWLKGEADADSLALKPLEFYAEHGIDFRP
GVKAVQLKRSDRTVVLSDGSTLAYDVLIIATGARPIALPIPGADLAGVMFLRTAADAEQLKAAVGPGKRLAVVGGGYIGL
EVAASGRALGAEVTVLEREPRLLARVACPELSTFFREYHEKHGVTFELGCSVTGFEGEAGRVTGVTLADGRTIACDAAVV
GVGAAPNDEIAADAGLETARGVVVDLDARTSDPAVFAIGDVAHRPMPIYDRMFRMESVPNALEQAKQAAAAITGRPRPPG
ECPWQWSDQYDLKLQIAGYPFDADQIVVRGDPATAKFAVFHLKGSQVQAVEAINAPPEFMMGKQLIANRKPVDPARLADP
TISMKEVAA

Sequences:

>Translated_409_residues
MSDATAHVVILGAGHAGGTAAALLRQYGHEGPITLVGEEPIPPYQRPPLSKAWLKGEADADSLALKPLEFYAEHGIDFRP
GVKAVQLKRSDRTVVLSDGSTLAYDVLIIATGARPIALPIPGADLAGVMFLRTAADAEQLKAAVGPGKRLAVVGGGYIGL
EVAASGRALGAEVTVLEREPRLLARVACPELSTFFREYHEKHGVTFELGCSVTGFEGEAGRVTGVTLADGRTIACDAAVV
GVGAAPNDEIAADAGLETARGVVVDLDARTSDPAVFAIGDVAHRPMPIYDRMFRMESVPNALEQAKQAAAAITGRPRPPG
ECPWQWSDQYDLKLQIAGYPFDADQIVVRGDPATAKFAVFHLKGSQVQAVEAINAPPEFMMGKQLIANRKPVDPARLADP
TISMKEVAA
>Mature_408_residues
SDATAHVVILGAGHAGGTAAALLRQYGHEGPITLVGEEPIPPYQRPPLSKAWLKGEADADSLALKPLEFYAEHGIDFRPG
VKAVQLKRSDRTVVLSDGSTLAYDVLIIATGARPIALPIPGADLAGVMFLRTAADAEQLKAAVGPGKRLAVVGGGYIGLE
VAASGRALGAEVTVLEREPRLLARVACPELSTFFREYHEKHGVTFELGCSVTGFEGEAGRVTGVTLADGRTIACDAAVVG
VGAAPNDEIAADAGLETARGVVVDLDARTSDPAVFAIGDVAHRPMPIYDRMFRMESVPNALEQAKQAAAAITGRPRPPGE
CPWQWSDQYDLKLQIAGYPFDADQIVVRGDPATAKFAVFHLKGSQVQAVEAINAPPEFMMGKQLIANRKPVDPARLADPT
ISMKEVAA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=380, Percent_Identity=30.2631578947368, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI65787454, Length=380, Percent_Identity=30.2631578947368, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI226437568, Length=380, Percent_Identity=30.2631578947368, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=28.7833827893175, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=28.7833827893175, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=35, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789065, Length=276, Percent_Identity=28.6231884057971, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789765, Length=254, Percent_Identity=34.6456692913386, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786307, Length=196, Percent_Identity=33.1632653061224, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=371, Percent_Identity=32.0754716981132, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32564386, Length=281, Percent_Identity=30.2491103202847, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=226, Percent_Identity=29.646017699115, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639257, Length=398, Percent_Identity=26.8844221105528, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI281359715, Length=398, Percent_Identity=26.8844221105528, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281359713, Length=398, Percent_Identity=26.8844221105528, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24639250, Length=398, Percent_Identity=26.8844221105528, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18543267, Length=398, Percent_Identity=26.8844221105528, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24639252, Length=398, Percent_Identity=26.8844221105528, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24585130, Length=401, Percent_Identity=24.4389027431421, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI28573993, Length=367, Percent_Identity=27.5204359673025, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581020, Length=367, Percent_Identity=27.5204359673025, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358499, Length=215, Percent_Identity=33.0232558139535, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 43095; Mature: 42963

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDATAHVVILGAGHAGGTAAALLRQYGHEGPITLVGEEPIPPYQRPPLSKAWLKGEADA
CCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCC
DSLALKPLEFYAEHGIDFRPGVKAVQLKRSDRTVVLSDGSTLAYDVLIIATGARPIALPI
CCEEECHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCEEEEEEEEEEECCCEEEEEC
PGADLAGVMFLRTAADAEQLKAAVGPGKRLAVVGGGYIGLEVAASGRALGAEVTVLEREP
CCCCHHHHEEEHHHCCHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCCCCEEEEEECCC
RLLARVACPELSTFFREYHEKHGVTFELGCSVTGFEGEAGRVTGVTLADGRTIACDAAVV
HHHHHHHCCHHHHHHHHHHHHCCCEEEECCEEECCCCCCCEEEEEEEECCCEEEECEEEE
GVGAAPNDEIAADAGLETARGVVVDLDARTSDPAVFAIGDVAHRPMPIYDRMFRMESVPN
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCHH
ALEQAKQAAAAITGRPRPPGECPWQWSDQYDLKLQIAGYPFDADQIVVRGDPATAKFAVF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEEE
HLKGSQVQAVEAINAPPEFMMGKQLIANRKPVDPARLADPTISMKEVAA
EECCCCEEEEHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHCC
>Mature Secondary Structure 
SDATAHVVILGAGHAGGTAAALLRQYGHEGPITLVGEEPIPPYQRPPLSKAWLKGEADA
CCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCC
DSLALKPLEFYAEHGIDFRPGVKAVQLKRSDRTVVLSDGSTLAYDVLIIATGARPIALPI
CCEEECHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCEEEEEEEEEEECCCEEEEEC
PGADLAGVMFLRTAADAEQLKAAVGPGKRLAVVGGGYIGLEVAASGRALGAEVTVLEREP
CCCCHHHHEEEHHHCCHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCCCCEEEEEECCC
RLLARVACPELSTFFREYHEKHGVTFELGCSVTGFEGEAGRVTGVTLADGRTIACDAAVV
HHHHHHHCCHHHHHHHHHHHHCCCEEEECCEEECCCCCCCEEEEEEEECCCEEEECEEEE
GVGAAPNDEIAADAGLETARGVVVDLDARTSDPAVFAIGDVAHRPMPIYDRMFRMESVPN
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCHH
ALEQAKQAAAAITGRPRPPGECPWQWSDQYDLKLQIAGYPFDADQIVVRGDPATAKFAVF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEEE
HLKGSQVQAVEAINAPPEFMMGKQLIANRKPVDPARLADPTISMKEVAA
EECCCCEEEEHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]