Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 197103662

GI number: 197103662

Start: 189139

End: 189972

Strand: Direct

Name: trpA

Synonym: PHZ_c0196

Alternate gene names: 197103662

Gene position: 189139-189972 (Clockwise)

Preceding gene: 197103661

Following gene: 197103663

Centisome position: 4.73

GC content: 71.7

Gene sequence:

>834_bases
TTGACCAAGGCCCGCATCGACGCCCGCTTCGCCGAACTCGCGCGCGAGGGCCGCGCGGCCTTCGTGCCCTATGTGATGGC
CGGCGATCCCGACCGGGAGACGGCGCTCGCCATCCTGAAGGGCCTGCCCGCGGCGGGGGCGGACATCATCGAGCTGGGCT
TCCCGTTCTCGGACCCGATGGCCGAGGGGCCGCCGATCCAGCGCGCCGCGGGCCGGGCGCTCGCCAAGGGCATGACGCTG
CAGGGCGTGCTGGACTTGGTCCGCGCCTTCCGCGCGGGGGACGCGACGACGCCGCTGATCCTGATGGGCTACATGAACCC
CCTCGTCACCTGGGGGCTGGAGGCCTTCGCCCGCGACGCCTCGGACGCCGGGGTGGACGGGCTGATCATCGTGGACTGCC
CGCCCGAGGAGGCCGATCCGCTGGCCGACGCCCTGGACGCGGCGGGCGTCTCGCTGATCCGGCTGGCGACGCCGACGACC
GGCGATGCGCGGCTGAAGGTGGTGGTCCGGCGGACCTCGGGCTTCGTCTACTACGTCTCGGTGGCCGGGGTGACGGGAGT
GAAGGAGGCCGACGCGGCCGCCGTCGCGCCCAACGTGGAGCGGGTCCGCAAGGCCTCGGGCCTGCCCGTCGCGGTCGGCT
TCGGCATCAAGACTCCGGAAAGGGCCGCGCAGGTAGCCCAGGTCGCCGACGCGGTCGTGGTGGGTTCCGCGCTTGTGGAC
GAAGTCGCCGAAGCAGTGGACATGAACGAAGACGTGACCGCGCGAGTTCTTTCCAAAGTCGAATCCTTGGCTAAGGCTGT
GCGCCTTGCGCGTCGTAGCCACGAGGCGGTCTGA

Upstream 100 bases:

>100_bases
TCACCCGCGAGGTGGGCAAGGACGGGATCGTCGTGCTGAACCTGTCGGGCCGCGGCGACAAGGACGTGAACACCGTCGCG
AGCTACCTGGGGCGGCAGAT

Downstream 100 bases:

>100_bases
AAACCATGGCGATGGCTGATCAGCGTAACGACAAGCCCGGCCGTCCGGCCGCCCAGCGGGAGCGCCGCGGCTGGCTGTCC
CGCATCGCGCCCGGCGTGCG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTKARIDARFAELAREGRAAFVPYVMAGDPDRETALAILKGLPAAGADIIELGFPFSDPMAEGPPIQRAAGRALAKGMTL
QGVLDLVRAFRAGDATTPLILMGYMNPLVTWGLEAFARDASDAGVDGLIIVDCPPEEADPLADALDAAGVSLIRLATPTT
GDARLKVVVRRTSGFVYYVSVAGVTGVKEADAAAVAPNVERVRKASGLPVAVGFGIKTPERAAQVAQVADAVVVGSALVD
EVAEAVDMNEDVTARVLSKVESLAKAVRLARRSHEAV

Sequences:

>Translated_277_residues
MTKARIDARFAELAREGRAAFVPYVMAGDPDRETALAILKGLPAAGADIIELGFPFSDPMAEGPPIQRAAGRALAKGMTL
QGVLDLVRAFRAGDATTPLILMGYMNPLVTWGLEAFARDASDAGVDGLIIVDCPPEEADPLADALDAAGVSLIRLATPTT
GDARLKVVVRRTSGFVYYVSVAGVTGVKEADAAAVAPNVERVRKASGLPVAVGFGIKTPERAAQVAQVADAVVVGSALVD
EVAEAVDMNEDVTARVLSKVESLAKAVRLARRSHEAV
>Mature_276_residues
TKARIDARFAELAREGRAAFVPYVMAGDPDRETALAILKGLPAAGADIIELGFPFSDPMAEGPPIQRAAGRALAKGMTLQ
GVLDLVRAFRAGDATTPLILMGYMNPLVTWGLEAFARDASDAGVDGLIIVDCPPEEADPLADALDAAGVSLIRLATPTTG
DARLKVVVRRTSGFVYYVSVAGVTGVKEADAAAVAPNVERVRKASGLPVAVGFGIKTPERAAQVAQVADAVVVGSALVDE
VAEAVDMNEDVTARVLSKVESLAKAVRLARRSHEAV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=265, Percent_Identity=32.8301886792453, Blast_Score=141, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=44.7257383966245, Blast_Score=195, Evalue=6e-51,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_PHEZH (B4RCL1)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129039.1
- GeneID:   6789686
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0196
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28814; Mature: 28683

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 51-51 ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKARIDARFAELAREGRAAFVPYVMAGDPDRETALAILKGLPAAGADIIELGFPFSDPM
CCCHHHHHHHHHHHHCCCCEEECEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCC
AEGPPIQRAAGRALAKGMTLQGVLDLVRAFRAGDATTPLILMGYMNPLVTWGLEAFARDA
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
SDAGVDGLIIVDCPPEEADPLADALDAAGVSLIRLATPTTGDARLKVVVRRTSGFVYYVS
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCEEEEEE
VAGVTGVKEADAAAVAPNVERVRKASGLPVAVGFGIKTPERAAQVAQVADAVVVGSALVD
ECCCCCCCCCCCHHCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EVAEAVDMNEDVTARVLSKVESLAKAVRLARRSHEAV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKARIDARFAELAREGRAAFVPYVMAGDPDRETALAILKGLPAAGADIIELGFPFSDPM
CCHHHHHHHHHHHHCCCCEEECEEECCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCC
AEGPPIQRAAGRALAKGMTLQGVLDLVRAFRAGDATTPLILMGYMNPLVTWGLEAFARDA
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
SDAGVDGLIIVDCPPEEADPLADALDAAGVSLIRLATPTTGDARLKVVVRRTSGFVYYVS
CCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCEEEEEE
VAGVTGVKEADAAAVAPNVERVRKASGLPVAVGFGIKTPERAAQVAQVADAVVVGSALVD
ECCCCCCCCCCCHHCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EVAEAVDMNEDVTARVLSKVESLAKAVRLARRSHEAV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA