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The map label for this gene is trpB

Identifier: 197103661

GI number: 197103661

Start: 187922

End: 189142

Strand: Direct

Name: trpB

Synonym: PHZ_c0195

Alternate gene names: 197103661

Gene position: 187922-189142 (Clockwise)

Preceding gene: 197103660

Following gene: 197103662

Centisome position: 4.7

GC content: 69.86

Gene sequence:

>1221_bases
TTGAACGCGCCTTCCCGTCCGAACGACTACGCGGCCTATCCCGACCTCAAGGGGCGGTTCGGCGACTACGGCGGCCAGTA
CGTGCCGGAAACCCTGATGCCGCTGGTGCACGAGCTGACCGCCGCCTACGAGGCGGCCAAGGCCGACCCCGCCTTCCAGG
CCGAGCTGGCCGGCTACCTGACCCACTACGTGGGCCGGCCCTCGCCGCTCTATTTCGCCGAGCGCCTGACGAGGCACTAC
GGCGGGGCGAAGATCTATCTCAAGCGGGAGGAGCTGAACCACACCGGCTCGCACAAGATCAACAACTGCATGGGCCAGAT
CCTGCTGGCCCAGCGGATGGGCAAGACGCGGATCATCGCCGAGACCGGCGCCGGCCAGCACGGCGTCGCCACGGCCACCG
TCTGCGCCCGCTTCGGCCTGCCGTGCGTGGTCTACATGGGCGCGGTGGACGTCGAGCGGCAGAAGCCGAACGTGTTCCGC
ATGAACCTGCTGGGCGCCGAGGTGCGGCCGGTGACCAGCGGATCGGCCACCCTGAAGGACGCCATGAACGAGGCGCTCCG
CGACTGGGTCACCAACGTCCACGACACCTATTACCTGATCGGCTCGGCGGCGGGGATGCACCCCTATCCGATGATGGTCC
GCGACTTCCAGGCGGTGATCGGCCGCGAGACGCGCGAGCAGATCCTGGAGCTGGAGGGCCGCCTGCCCGACGCGCTGGTC
GCCTGCGTCGGCGGCGGCTCGAACGCCATCGGCCTGTTCCACCCGTTCCTGAACGACTCCTCGGTGAAGATCTTCGGCGT
CGAGGCGGCGGGCGAGGGGGTCGAGACCGGCCGCCACGCCGCAGCGATCAACGGCGGCCGCCCCGGCGTGCTGCACGGGA
ACATGACCTACCTGCTGCAGGACCGGGTGGGGCAGATCGAGGAGGCGCACTCCATCTCGGCCGGCCTCGACTATCCGGGC
ATCGGCCCCGAGCACGCCTGGCTGCACGACGTGGGCCGCGCCACCTACCTGACCGCCACCGACACGGAGGCGCTGGAGGC
GTTCCGCCTGCTGTCCGAGCTGGAGGGCATCCTGCCGGCGATCGAGAGCTCGCACGCCCTGGCCCGCCTGCCCGAGATCA
CCCGCGAGGTGGGCAAGGACGGGATCGTCGTGCTGAACCTGTCGGGCCGCGGCGACAAGGACGTGAACACCGTCGCGAGC
TACCTGGGGCGGCAGATTTGA

Upstream 100 bases:

>100_bases
GTGGACGTTTCCTCTGGCGTGGAGCGCGGCCCCGGACTAAAGGACCCCACCTTGATCCGGGCGTTCCTCGACGCCGTCAG
ACGCGCCTAAGAGCCTCGTT

Downstream 100 bases:

>100_bases
CCAAGGCCCGCATCGACGCCCGCTTCGCCGAACTCGCGCGCGAGGGCCGCGCGGCCTTCGTGCCCTATGTGATGGCCGGC
GATCCCGACCGGGAGACGGC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNAPSRPNDYAAYPDLKGRFGDYGGQYVPETLMPLVHELTAAYEAAKADPAFQAELAGYLTHYVGRPSPLYFAERLTRHY
GGAKIYLKREELNHTGSHKINNCMGQILLAQRMGKTRIIAETGAGQHGVATATVCARFGLPCVVYMGAVDVERQKPNVFR
MNLLGAEVRPVTSGSATLKDAMNEALRDWVTNVHDTYYLIGSAAGMHPYPMMVRDFQAVIGRETREQILELEGRLPDALV
ACVGGGSNAIGLFHPFLNDSSVKIFGVEAAGEGVETGRHAAAINGGRPGVLHGNMTYLLQDRVGQIEEAHSISAGLDYPG
IGPEHAWLHDVGRATYLTATDTEALEAFRLLSELEGILPAIESSHALARLPEITREVGKDGIVVLNLSGRGDKDVNTVAS
YLGRQI

Sequences:

>Translated_406_residues
MNAPSRPNDYAAYPDLKGRFGDYGGQYVPETLMPLVHELTAAYEAAKADPAFQAELAGYLTHYVGRPSPLYFAERLTRHY
GGAKIYLKREELNHTGSHKINNCMGQILLAQRMGKTRIIAETGAGQHGVATATVCARFGLPCVVYMGAVDVERQKPNVFR
MNLLGAEVRPVTSGSATLKDAMNEALRDWVTNVHDTYYLIGSAAGMHPYPMMVRDFQAVIGRETREQILELEGRLPDALV
ACVGGGSNAIGLFHPFLNDSSVKIFGVEAAGEGVETGRHAAAINGGRPGVLHGNMTYLLQDRVGQIEEAHSISAGLDYPG
IGPEHAWLHDVGRATYLTATDTEALEAFRLLSELEGILPAIESSHALARLPEITREVGKDGIVVLNLSGRGDKDVNTVAS
YLGRQI
>Mature_406_residues
MNAPSRPNDYAAYPDLKGRFGDYGGQYVPETLMPLVHELTAAYEAAKADPAFQAELAGYLTHYVGRPSPLYFAERLTRHY
GGAKIYLKREELNHTGSHKINNCMGQILLAQRMGKTRIIAETGAGQHGVATATVCARFGLPCVVYMGAVDVERQKPNVFR
MNLLGAEVRPVTSGSATLKDAMNEALRDWVTNVHDTYYLIGSAAGMHPYPMMVRDFQAVIGRETREQILELEGRLPDALV
ACVGGGSNAIGLFHPFLNDSSVKIFGVEAAGEGVETGRHAAAINGGRPGVLHGNMTYLLQDRVGQIEEAHSISAGLDYPG
IGPEHAWLHDVGRATYLTATDTEALEAFRLLSELEGILPAIESSHALARLPEITREVGKDGIVVLNLSGRGDKDVNTVAS
YLGRQI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=384, Percent_Identity=57.03125, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321412, Length=387, Percent_Identity=59.1731266149871, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_PHEZH (B4RCL0)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129038.1
- GeneID:   6789685
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0195
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43939; Mature: 43939

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPSRPNDYAAYPDLKGRFGDYGGQYVPETLMPLVHELTAAYEAAKADPAFQAELAGYL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
THYVGRPSPLYFAERLTRHYGGAKIYLKREELNHTGSHKINNCMGQILLAQRMGKTRIIA
HHHCCCCCHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
ETGAGQHGVATATVCARFGLPCVVYMGAVDVERQKPNVFRMNLLGAEVRPVTSGSATLKD
ECCCCCCCHHHHHHHHHHCCCCEEEECCEECCCCCCCEEEEEECCCCCCCCCCCCHHHHH
AMNEALRDWVTNVHDTYYLIGSAAGMHPYPMMVRDFQAVIGRETREQILELEGRLPDALV
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHE
ACVGGGSNAIGLFHPFLNDSSVKIFGVEAAGEGVETGRHAAAINGGRPGVLHGNMTYLLQ
EEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCEEECCHHHEEH
DRVGQIEEAHSISAGLDYPGIGPEHAWLHDVGRATYLTATDTEALEAFRLLSELEGILPA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
IESSHALARLPEITREVGKDGIVVLNLSGRGDKDVNTVASYLGRQI
HHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNAPSRPNDYAAYPDLKGRFGDYGGQYVPETLMPLVHELTAAYEAAKADPAFQAELAGYL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
THYVGRPSPLYFAERLTRHYGGAKIYLKREELNHTGSHKINNCMGQILLAQRMGKTRIIA
HHHCCCCCHHHHHHHHHHHCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
ETGAGQHGVATATVCARFGLPCVVYMGAVDVERQKPNVFRMNLLGAEVRPVTSGSATLKD
ECCCCCCCHHHHHHHHHHCCCCEEEECCEECCCCCCCEEEEEECCCCCCCCCCCCHHHHH
AMNEALRDWVTNVHDTYYLIGSAAGMHPYPMMVRDFQAVIGRETREQILELEGRLPDALV
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHE
ACVGGGSNAIGLFHPFLNDSSVKIFGVEAAGEGVETGRHAAAINGGRPGVLHGNMTYLLQ
EEECCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCEEECCHHHEEH
DRVGQIEEAHSISAGLDYPGIGPEHAWLHDVGRATYLTATDTEALEAFRLLSELEGILPA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
IESSHALARLPEITREVGKDGIVVLNLSGRGDKDVNTVASYLGRQI
HHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA