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The map label for this gene is mdh [H]

Identifier: 197103618

GI number: 197103618

Start: 141453

End: 142442

Strand: Direct

Name: mdh [H]

Synonym: PHZ_c0152

Alternate gene names: 197103618

Gene position: 141453-142442 (Clockwise)

Preceding gene: 197103616

Following gene: 197103621

Centisome position: 3.54

GC content: 68.48

Gene sequence:

>990_bases
ATGGCCTCGAAGCCGCCCATCCGAGTCGCCGTCACCGGGGCCGCCGGCAACATCGGCTACGCCCTGCTCTTCCGCATCGC
CTCGGGCGAGATGCTGGGCAAGGACCAGCCGGTCATCCTGCAGCTCCTGGAGATCCCCGCCGAGGGCCCGCAGAAGGCGC
TCAAGGGCGTGGCCATGGAGCTCGAGGACTGCGCCTTCCCGCTGCTGCAGGACATGGTGCTGACCGACGACGCTAACGTC
GCCTTCAAGGACGCCAACTGGTGCCTGCTGGTGGGCTCCAAGCCCCGTGGCCCCGGCATGGAGCGCGCCGACCTGCTGAA
GGACAACGGCAAGATCTTCATCGCCCAGGGCAAGGCCATCGACGCCGTGGCCGCCGATGACGCCCGCGTCGCGGTGGTCG
GCAACCCCTGCAACACCAACGTCATGATCGCCGCCAGCCAGGCCAAGCGCCTGACGCCGGACCGCTTCACCGCCATGGTG
CGCCTCGACGAGAACCGCGGCCGCGCGCAGCTCGCCAAGAAGGCCGGCGTGTCGATCAACGACGTCTCCGAGCTGTTCAT
CTACGGCAACCACAGCCCGACGATGTTCGCCGACTTCACCCACGCCAAGATCGGCGGCAAGGCGGCGGCGGACGTGATCA
ACGACCGCGCCTGGCTCGAGAACGAGTACCTGCCCACCGTCGGCAAGCGCGGCGCGGCGATCATCGAGGCCCGGGGCCAG
AGCTCGGCGGCCTCGGCGGCCAACGCCCTGATCGACCACGTGCGCAGCCTGAACACCGTGGGCGAGGTCCACTCGATCGC
GGTGAACTCGGAAGGCCGCTACGGCTTCGCCGAGGGCGTGTGGGCCGGCATGCCGGTGAAGACCACCGCGCCGGGCGCCT
ACGAGGTCGTCACCAGCTACGAGATGGACGACTTCGCCAAGTCGAAGATCAAGATCACCAACGACGAGCTGGTCGCCGAG
CGCGACACCGTCAAGGACATGCTGGGCTAA

Upstream 100 bases:

>100_bases
TCGCTCCAGGGCCTGCGGCATTGCAGCATCGCCGTCAATTGGCTACGGACCCGGCGCGCAGCCGCGTGTTTTCGCCACGA
GTTTTGGAAGGAGCCGTCCC

Downstream 100 bases:

>100_bases
GGCTTCACGGCAGCGCCCGTTGTGGAAGGGCCTCCGGCGCCGCCGGGGGCCCTTTCCTTTTGCGCGCTAGTAGTCCGCCA
GGTCGTCCAGCTCGGCGATC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MASKPPIRVAVTGAAGNIGYALLFRIASGEMLGKDQPVILQLLEIPAEGPQKALKGVAMELEDCAFPLLQDMVLTDDANV
AFKDANWCLLVGSKPRGPGMERADLLKDNGKIFIAQGKAIDAVAADDARVAVVGNPCNTNVMIAASQAKRLTPDRFTAMV
RLDENRGRAQLAKKAGVSINDVSELFIYGNHSPTMFADFTHAKIGGKAAADVINDRAWLENEYLPTVGKRGAAIIEARGQ
SSAASAANALIDHVRSLNTVGEVHSIAVNSEGRYGFAEGVWAGMPVKTTAPGAYEVVTSYEMDDFAKSKIKITNDELVAE
RDTVKDMLG

Sequences:

>Translated_329_residues
MASKPPIRVAVTGAAGNIGYALLFRIASGEMLGKDQPVILQLLEIPAEGPQKALKGVAMELEDCAFPLLQDMVLTDDANV
AFKDANWCLLVGSKPRGPGMERADLLKDNGKIFIAQGKAIDAVAADDARVAVVGNPCNTNVMIAASQAKRLTPDRFTAMV
RLDENRGRAQLAKKAGVSINDVSELFIYGNHSPTMFADFTHAKIGGKAAADVINDRAWLENEYLPTVGKRGAAIIEARGQ
SSAASAANALIDHVRSLNTVGEVHSIAVNSEGRYGFAEGVWAGMPVKTTAPGAYEVVTSYEMDDFAKSKIKITNDELVAE
RDTVKDMLG
>Mature_328_residues
ASKPPIRVAVTGAAGNIGYALLFRIASGEMLGKDQPVILQLLEIPAEGPQKALKGVAMELEDCAFPLLQDMVLTDDANVA
FKDANWCLLVGSKPRGPGMERADLLKDNGKIFIAQGKAIDAVAADDARVAVVGNPCNTNVMIAASQAKRLTPDRFTAMVR
LDENRGRAQLAKKAGVSINDVSELFIYGNHSPTMFADFTHAKIGGKAAADVINDRAWLENEYLPTVGKRGAAIIEARGQS
SAASAANALIDHVRSLNTVGEVHSIAVNSEGRYGFAEGVWAGMPVKTTAPGAYEVVTSYEMDDFAKSKIKITNDELVAER
DTVKDMLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=332, Percent_Identity=45.7831325301205, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI89886456, Length=335, Percent_Identity=21.1940298507463, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17561064, Length=325, Percent_Identity=46.1538461538462, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI32566798, Length=255, Percent_Identity=48.2352941176471, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI32566800, Length=171, Percent_Identity=43.2748538011696, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583394, Length=330, Percent_Identity=45.4545454545455, Blast_Score=260, Evalue=7e-70,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35030; Mature: 34899

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKPPIRVAVTGAAGNIGYALLFRIASGEMLGKDQPVILQLLEIPAEGPQKALKGVAME
CCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHH
LEDCAFPLLQDMVLTDDANVAFKDANWCLLVGSKPRGPGMERADLLKDNGKIFIAQGKAI
HHHHHHHHHHHHHEECCCCEEEECCCEEEEECCCCCCCCCCHHHHHCCCCEEEEEECCEE
DAVAADDARVAVVGNPCNTNVMIAASQAKRLTPDRFTAMVRLDENRGRAQLAKKAGVSIN
EEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCC
DVSELFIYGNHSPTMFADFTHAKIGGKAAADVINDRAWLENEYLPTVGKRGAAIIEARGQ
CCEEEEEEECCCCCEEEECHHHCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEECCC
SSAASAANALIDHVRSLNTVGEVHSIAVNSEGRYGFAEGVWAGMPVKTTAPGAYEVVTSY
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
EMDDFAKSKIKITNDELVAERDTVKDMLG
CHHHHCCCEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASKPPIRVAVTGAAGNIGYALLFRIASGEMLGKDQPVILQLLEIPAEGPQKALKGVAME
CCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHH
LEDCAFPLLQDMVLTDDANVAFKDANWCLLVGSKPRGPGMERADLLKDNGKIFIAQGKAI
HHHHHHHHHHHHHEECCCCEEEECCCEEEEECCCCCCCCCCHHHHHCCCCEEEEEECCEE
DAVAADDARVAVVGNPCNTNVMIAASQAKRLTPDRFTAMVRLDENRGRAQLAKKAGVSIN
EEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCC
DVSELFIYGNHSPTMFADFTHAKIGGKAAADVINDRAWLENEYLPTVGKRGAAIIEARGQ
CCEEEEEEECCCCCEEEECHHHCCCCHHHHHHHCCHHHHCCCCCCCCCCCCCEEEEECCC
SSAASAANALIDHVRSLNTVGEVHSIAVNSEGRYGFAEGVWAGMPVKTTAPGAYEVVTSY
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
EMDDFAKSKIKITNDELVAERDTVKDMLG
CHHHHCCCEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA