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The map label for this gene is mcl2 [H]

Identifier: 197103616

GI number: 197103616

Start: 139548

End: 140417

Strand: Direct

Name: mcl2 [H]

Synonym: PHZ_c0150

Alternate gene names: 197103616

Gene position: 139548-140417 (Clockwise)

Preceding gene: 197103613

Following gene: 197103618

Centisome position: 3.49

GC content: 74.02

Gene sequence:

>870_bases
ATGTCCGCTCTCGCCCGTCCCCGCCGCAGCGCGCTCTACCTGCCCGCCTCCAACGCCCGCGCGATCGAGAAGGCCCGGGA
GCTGGCCTGCGACGTGGTGATCCTCGACCTGGAGGACGCCGTCGCCCCCGAGCAGAAGGAGGAGGCCCGCGCCCAGGCCG
TGGCCGCCGTGAAGGCCGGCGGCTTCGGCCCGCGCGAGGTGGTGATCCGCGCCAACGGCCTCGACACCCCCTGGGGCGCC
GAGGACCTGGCCGCCGCCGCCCAGGCCGGGCCGGACGCGGTCCTGGTCCCGAAGGTGTCCTCGGCCGAGGACCTCGCCGC
CTGTGCCGGGGCCGCCGGGAGCCTGCGGATCTGGGCGATGATCGAGACCTGCCAGGCCATGCTGCGCCTGGACGCCCTGG
CCGGCGCCTCGCGCGCGCATCGCACGGACGTGTGGGTGATCGGCACGAACGACCTGGCCAAGGAGATGCGCTGCCCGCTC
ACGCCGGAACGCACGCCCCTGCTGCCGGCGCTCAGCTTTGCAGTGATGGCGGCGCGGGCCCACGGCCTGTCGATCCTCGA
CGGCGTCTACAACGAGATCGCCGACGCCGAAGGCCTTGCGGCGCAGTGCGCCCAGGCCCTGGCGTTCGGCTTCGACGGCA
AGACCCTGATCCACCCTTCGCAGATCGAAGCGGCCAATCGCGCGTTCACTCCCGAGCCCGGCGCCGTCGCCTGGGCGCGC
ACGATCGTCTCGGCCTTCGAAGCGCCGCAGAACGCCCACCGCGGCGTGCTGAAGGTGGAGGGCCGGATGGTCGAGCGCTT
GCATCTGGCCGAGGCCCGGCGCCTGATCGCCGTGGCCGACGCCATCGCCGCGCACGAGGGAGGCGGATGA

Upstream 100 bases:

>100_bases
GGGCGCCGGCGTCGTAGCGGAGCGGGCGGATGGCCATGGGCGCATCCTAAACGCTAAGACGGGGCGTCCGCCAAGGCGCT
GAAGGAGCCTCGTCCGCCCA

Downstream 100 bases:

>100_bases
GGACGAGGGCAGGAACGATCGCAGCCGGACTGCCGGCGCTGATAGTCGCGCTCATCGGGGCCGCGCCCGCGGCCGCGCAA
CCCGCGGCCGATCCCATCGG

Product: citrate lyase beta subunit

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSALARPRRSALYLPASNARAIEKARELACDVVILDLEDAVAPEQKEEARAQAVAAVKAGGFGPREVVIRANGLDTPWGA
EDLAAAAQAGPDAVLVPKVSSAEDLAACAGAAGSLRIWAMIETCQAMLRLDALAGASRAHRTDVWVIGTNDLAKEMRCPL
TPERTPLLPALSFAVMAARAHGLSILDGVYNEIADAEGLAAQCAQALAFGFDGKTLIHPSQIEAANRAFTPEPGAVAWAR
TIVSAFEAPQNAHRGVLKVEGRMVERLHLAEARRLIAVADAIAAHEGGG

Sequences:

>Translated_289_residues
MSALARPRRSALYLPASNARAIEKARELACDVVILDLEDAVAPEQKEEARAQAVAAVKAGGFGPREVVIRANGLDTPWGA
EDLAAAAQAGPDAVLVPKVSSAEDLAACAGAAGSLRIWAMIETCQAMLRLDALAGASRAHRTDVWVIGTNDLAKEMRCPL
TPERTPLLPALSFAVMAARAHGLSILDGVYNEIADAEGLAAQCAQALAFGFDGKTLIHPSQIEAANRAFTPEPGAVAWAR
TIVSAFEAPQNAHRGVLKVEGRMVERLHLAEARRLIAVADAIAAHEGGG
>Mature_288_residues
SALARPRRSALYLPASNARAIEKARELACDVVILDLEDAVAPEQKEEARAQAVAAVKAGGFGPREVVIRANGLDTPWGAE
DLAAAAQAGPDAVLVPKVSSAEDLAACAGAAGSLRIWAMIETCQAMLRLDALAGASRAHRTDVWVIGTNDLAKEMRCPLT
PERTPLLPALSFAVMAARAHGLSILDGVYNEIADAEGLAAQCAQALAFGFDGKTLIHPSQIEAANRAFTPEPGAVAWART
IVSAFEAPQNAHRGVLKVEGRMVERLHLAEARRLIAVADAIAAHEGGG

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=294, Percent_Identity=29.2517006802721, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87081764, Length=272, Percent_Identity=32.7205882352941, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17557458, Length=301, Percent_Identity=28.9036544850498, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30229; Mature: 30098

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALARPRRSALYLPASNARAIEKARELACDVVILDLEDAVAPEQKEEARAQAVAAVKAG
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEEEECHHHCCCCHHHHHHHHHHHHHHCC
GFGPREVVIRANGLDTPWGAEDLAAAAQAGPDAVLVPKVSSAEDLAACAGAAGSLRIWAM
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEHHHH
IETCQAMLRLDALAGASRAHRTDVWVIGTNDLAKEMRCPLTPERTPLLPALSFAVMAARA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
HGLSILDGVYNEIADAEGLAAQCAQALAFGFDGKTLIHPSQIEAANRAFTPEPGAVAWAR
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECHHHHHHHCCCCCCCCCHHHHHH
TIVSAFEAPQNAHRGVLKVEGRMVERLHLAEARRLIAVADAIAAHEGGG
HHHHHHHCCHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SALARPRRSALYLPASNARAIEKARELACDVVILDLEDAVAPEQKEEARAQAVAAVKAG
CCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEEEECHHHCCCCHHHHHHHHHHHHHHCC
GFGPREVVIRANGLDTPWGAEDLAAAAQAGPDAVLVPKVSSAEDLAACAGAAGSLRIWAM
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEHHHH
IETCQAMLRLDALAGASRAHRTDVWVIGTNDLAKEMRCPLTPERTPLLPALSFAVMAARA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
HGLSILDGVYNEIADAEGLAAQCAQALAFGFDGKTLIHPSQIEAANRAFTPEPGAVAWAR
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECHHHHHHHCCCCCCCCCHHHHHH
TIVSAFEAPQNAHRGVLKVEGRMVERLHLAEARRLIAVADAIAAHEGGG
HHHHHHHCCHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA