Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 197103578

GI number: 197103578

Start: 102759

End: 103469

Strand: Reverse

Name: trmD [H]

Synonym: PHZ_c0112

Alternate gene names: 197103578

Gene position: 103469-102759 (Counterclockwise)

Preceding gene: 197103581

Following gene: 197103577

Centisome position: 2.59

GC content: 69.48

Gene sequence:

>711_bases
ATGTCCTTCGACTGCGCCGTACTGACCATGTTCCCCGAGGCCTTCCCCGGCCCGCTGGGCGTCTCGCTCATCGGCACTGC
CTGGCGCGAGCAGAACCTCTGGTCGCTCGAAACGGTGGACATCCGGGCCTTTTCCAAGGATAAGCGCGGCTTCCTCGACG
ACACCCCTGCGGGTGGCGGCCCGGGGCAGGTGATGCGGGCCGACGTGATCGCCTCCGCGCTGGACAGCGTGGAGCGGCGC
GGACGTCCGCTTTTGTACATGAGCGCCCGGGGCCGGCCCCTGACCCAGGCGCGCGTGAGTGAATGGGCCGAGGGCCCGGG
GCTGATCGTCCTGTGCGGTCGGTTCGAAGGGGTGGACCAGCGGGTGCTCGACGCCCGCGGGTTCGAGGAGGTCTCCGTCG
GAGACGCGGTCCTGGCCGGAGGCGAGGCTGCGGCGATGGTGGCGATCGAGGCGTGCGTACGGCTCGTGCCCGGTGTGTTG
GGGCAGGCCGAGAGTTTGAGTGAGGAAAGCTTCGAGGACGGGCTCCTCGAGCATCCGCAGTACACCAGGCCGCGGACGTT
CGAAGGGCTCGAGATCCCCGAGGTGCTGCTTTCGGGCAATCACGCGGCCATCCGCCGCTGGCGCCAGGAGCAGCGCGAAC
AGACCACGCGGGAGCGGCGTCCGGACCTCTGGGCGAAGCGGCTCGCCAATCAACAGGCAAAGGGCGGGTAA

Upstream 100 bases:

>100_bases
TCTTGGGCGTCGGTCTCGCTCATGACCAGCGGCGATACGCGGGGGCGCCGCGCCCGGCAAGGCTTGACGTGGCGGCGTCG
GCGCCCGCCTTACGACCGCC

Downstream 100 bases:

>100_bases
AGCCCAGTAAGGAAGACGACGATGAACGTGATCGAACAGCTCCGTAAGGAAGAGGCCGACCGCCTCCTGGCGCAGCGCAA
GGTTCCGGAATTCCGCCCCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSFDCAVLTMFPEAFPGPLGVSLIGTAWREQNLWSLETVDIRAFSKDKRGFLDDTPAGGGPGQVMRADVIASALDSVERR
GRPLLYMSARGRPLTQARVSEWAEGPGLIVLCGRFEGVDQRVLDARGFEEVSVGDAVLAGGEAAAMVAIEACVRLVPGVL
GQAESLSEESFEDGLLEHPQYTRPRTFEGLEIPEVLLSGNHAAIRRWRQEQREQTTRERRPDLWAKRLANQQAKGG

Sequences:

>Translated_236_residues
MSFDCAVLTMFPEAFPGPLGVSLIGTAWREQNLWSLETVDIRAFSKDKRGFLDDTPAGGGPGQVMRADVIASALDSVERR
GRPLLYMSARGRPLTQARVSEWAEGPGLIVLCGRFEGVDQRVLDARGFEEVSVGDAVLAGGEAAAMVAIEACVRLVPGVL
GQAESLSEESFEDGLLEHPQYTRPRTFEGLEIPEVLLSGNHAAIRRWRQEQREQTTRERRPDLWAKRLANQQAKGG
>Mature_235_residues
SFDCAVLTMFPEAFPGPLGVSLIGTAWREQNLWSLETVDIRAFSKDKRGFLDDTPAGGGPGQVMRADVIASALDSVERRG
RPLLYMSARGRPLTQARVSEWAEGPGLIVLCGRFEGVDQRVLDARGFEEVSVGDAVLAGGEAAAMVAIEACVRLVPGVLG
QAESLSEESFEDGLLEHPQYTRPRTFEGLEIPEVLLSGNHAAIRRWRQEQREQTTRERRPDLWAKRLANQQAKGG

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=234, Percent_Identity=42.7350427350427, Blast_Score=176, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25875; Mature: 25743

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDCAVLTMFPEAFPGPLGVSLIGTAWREQNLWSLETVDIRAFSKDKRGFLDDTPAGGG
CCCCCCCEECCHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCC
PGQVMRADVIASALDSVERRGRPLLYMSARGRPLTQARVSEWAEGPGLIVLCGRFEGVDQ
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCHH
RVLDARGFEEVSVGDAVLAGGEAAAMVAIEACVRLVPGVLGQAESLSEESFEDGLLEHPQ
HHHHHCCCCHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
YTRPRTFEGLEIPEVLLSGNHAAIRRWRQEQREQTTRERRPDLWAKRLANQQAKGG
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFDCAVLTMFPEAFPGPLGVSLIGTAWREQNLWSLETVDIRAFSKDKRGFLDDTPAGGG
CCCCCCEECCHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCC
PGQVMRADVIASALDSVERRGRPLLYMSARGRPLTQARVSEWAEGPGLIVLCGRFEGVDQ
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCHH
RVLDARGFEEVSVGDAVLAGGEAAAMVAIEACVRLVPGVLGQAESLSEESFEDGLLEHPQ
HHHHHCCCCHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
YTRPRTFEGLEIPEVLLSGNHAAIRRWRQEQREQTTRERRPDLWAKRLANQQAKGG
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]