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The map label for this gene is agaD [H]

Identifier: 195978413

GI number: 195978413

Start: 1342804

End: 1343658

Strand: Direct

Name: agaD [H]

Synonym: Sez_1308

Alternate gene names: 195978413

Gene position: 1342804-1343658 (Clockwise)

Preceding gene: 195978412

Following gene: 195978414

Centisome position: 66.34

GC content: 41.99

Gene sequence:

>855_bases
ATGCAACAACAGCTGAACAAGGAGGTCAACAAGATGACTGGATCTAATAAGCTCACAAAGCGTGATTATCTGAAAACTGC
TCTTAGAGCCTTTTTCCTGCAAAATGGCTTTAACTATAGCAATTATCAAGGAATAGGCTATGCCAATGTCGTTTACCCAG
CCCTAAAAAAGCATTTTGGCAATGACAAAACAGGCCTTTACAAAGCACTAGAGGACAATTGTGCATTTTACAATACAAAC
CCTCATTTCCTACCTTTTATCACCAGCCTACACCTGGTCATGCTAGAAAATAATCGGCCTGAAGAAGAAACCCGCAATAT
CAAGATGGCACTCATGGGACCTCTAGCAGGCATCGGGGATTCCCTTTCGCAATTTTGTTTAGCACCACTCTTTTCGACCA
TTGCTGCTTCCTTAGCAAGTGACGGCCTGATTTTGGGGCCAATTCTCTTTTTCCTAGCCATGAATATTATTCTAACAGCT
ATTAAAATTGGCTCTGGACTCTATGGCTACAAGGTGGGGACAAGCTTTATTGATAAGCTAAGCGAACAAATGGCAATTGT
ATCTCGCATGGCAAATATTGTCGGAGTTACAGTGATTGCTGGTCTAGCTGCTACTTCTGTTAAGATATCTGTTCCCATCA
CATTTGCAGCAGGCAAGGTCGACGCTGCAAATGCAGCCCAAAAATTTGTGACCATTCAGGGAATGCTGGATAAGATTGCA
CCAGCTCTTCTGCCAGCCCTCTTCACTCTCTTGATGTATTACCTCATTAAAACGAAAAAATGGACAACCTATAAATTAGT
TATTCTAACAGTTATTATTGGTATTGTTGGCAGCTGGTTAGGGGTGTTAGCCTAG

Upstream 100 bases:

>100_bases
ATTGGTTATGTCTGTGCAGCTTACCTGCAAATTCCAACCATCGGTATTGCCATCATAGGCATTATCTTCGCCCTAAATGA
ATACTACAACAAACCAAAGC

Downstream 100 bases:

>100_bases
CAGTCTTAGAGATTCTGTTAATCAGTATTGGAGGGTCTCCATTAGTGATGAGCAGGTCTCTTTTCTATTGGGCAAAGCTA
AGCATTGACACCATCAACTC

Product: N-acetylgalactosamine permease IID component AgaD

Products: protein histidine; sugar phosphate

Alternate protein names: EIID-Aga; PTS system N-acetylgalactosamine-specific EIID component [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQQQLNKEVNKMTGSNKLTKRDYLKTALRAFFLQNGFNYSNYQGIGYANVVYPALKKHFGNDKTGLYKALEDNCAFYNTN
PHFLPFITSLHLVMLENNRPEEETRNIKMALMGPLAGIGDSLSQFCLAPLFSTIAASLASDGLILGPILFFLAMNIILTA
IKIGSGLYGYKVGTSFIDKLSEQMAIVSRMANIVGVTVIAGLAATSVKISVPITFAAGKVDAANAAQKFVTIQGMLDKIA
PALLPALFTLLMYYLIKTKKWTTYKLVILTVIIGIVGSWLGVLA

Sequences:

>Translated_284_residues
MQQQLNKEVNKMTGSNKLTKRDYLKTALRAFFLQNGFNYSNYQGIGYANVVYPALKKHFGNDKTGLYKALEDNCAFYNTN
PHFLPFITSLHLVMLENNRPEEETRNIKMALMGPLAGIGDSLSQFCLAPLFSTIAASLASDGLILGPILFFLAMNIILTA
IKIGSGLYGYKVGTSFIDKLSEQMAIVSRMANIVGVTVIAGLAATSVKISVPITFAAGKVDAANAAQKFVTIQGMLDKIA
PALLPALFTLLMYYLIKTKKWTTYKLVILTVIIGIVGSWLGVLA
>Mature_284_residues
MQQQLNKEVNKMTGSNKLTKRDYLKTALRAFFLQNGFNYSNYQGIGYANVVYPALKKHFGNDKTGLYKALEDNCAFYNTN
PHFLPFITSLHLVMLENNRPEEETRNIKMALMGPLAGIGDSLSQFCLAPLFSTIAASLASDGLILGPILFFLAMNIILTA
IKIGSGLYGYKVGTSFIDKLSEQMAIVSRMANIVGVTVIAGLAATSVKISVPITFAAGKVDAANAAQKFVTIQGMLDKIA
PALLPALFTLLMYYLIKTKKWTTYKLVILTVIIGIVGSWLGVLA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1789529, Length=268, Percent_Identity=37.6865671641791, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1788122, Length=290, Percent_Identity=26.8965517241379, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 30979; Mature: 30979

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQLNKEVNKMTGSNKLTKRDYLKTALRAFFLQNGFNYSNYQGIGYANVVYPALKKHFG
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
NDKTGLYKALEDNCAFYNTNPHFLPFITSLHLVMLENNRPEEETRNIKMALMGPLAGIGD
CCCHHHHHHHHCCCEEECCCCCHHHHHHHHEEEEECCCCCHHHHHCCHHHHHCCHHHCCH
SLSQFCLAPLFSTIAASLASDGLILGPILFFLAMNIILTAIKIGSGLYGYKVGTSFIDKL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SEQMAIVSRMANIVGVTVIAGLAATSVKISVPITFAAGKVDAANAAQKFVTIQGMLDKIA
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
PALLPALFTLLMYYLIKTKKWTTYKLVILTVIIGIVGSWLGVLA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQQLNKEVNKMTGSNKLTKRDYLKTALRAFFLQNGFNYSNYQGIGYANVVYPALKKHFG
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
NDKTGLYKALEDNCAFYNTNPHFLPFITSLHLVMLENNRPEEETRNIKMALMGPLAGIGD
CCCHHHHHHHHCCCEEECCCCCHHHHHHHHEEEEECCCCCHHHHHCCHHHHHCCHHHCCH
SLSQFCLAPLFSTIAASLASDGLILGPILFFLAMNIILTAIKIGSGLYGYKVGTSFIDKL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SEQMAIVSRMANIVGVTVIAGLAATSVKISVPITFAAGKVDAANAAQKFVTIQGMLDKIA
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
PALLPALFTLLMYYLIKTKKWTTYKLVILTVIIGIVGSWLGVLA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10931310; 9278503; 8932697 [H]