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The map label for this gene is trmE [H]

Identifier: 195978293

GI number: 195978293

Start: 1215018

End: 1216394

Strand: Direct

Name: trmE [H]

Synonym: Sez_1175

Alternate gene names: 195978293

Gene position: 1215018-1216394 (Clockwise)

Preceding gene: 195978279

Following gene: 195978309

Centisome position: 60.03

GC content: 43.72

Gene sequence:

>1377_bases
ATGAGTATAACTAAAGAATTTGATACAATTGCAGCCATTTCAACTCCGTTAGGTGAAGGAGCTATTGGCATTGTTCGCTT
GTCTGGTACCAAGGCTCTGGACATTGCTAAATCGATCTTTAAAGGAAAAGATCTAACAACAGTCGCCTCACATACCCTTA
ACTATGGTCATATTATCAAGCCAAGCACAGGTGAAGTGATTGATGAGGTCATGGTATCAGTCATGCTGGCGCCAAAAACC
TTTACCAGAGAGGACGTGATTGAAATCAATACCCATGGCGGTATTGCGGTGACAAATGACATTTTACAGCTCCTCATTAA
ACAGGGAGCTCGAATGGCAGAGCCCGGCGAATTTACAAAGCGTGCTTTTTTAAATGGCCGTATTGATTTAACACAGGCCG
AAGCGGTTATGGATCTGATCCGTGCAAAGACTGATAAGGCCATGTCTATTGCTATCAAGCAGCTAGATGGCTCCCTATCA
CAGCTCATCAGCGATACACGTCAAGAAATCCTAAATACCTTAGCCCAGGTTGAGGTCAATATCGACTACCCCGAATATGA
TGATGTCGAAGAAATGACCACTGCTCTTTTGCGTGACAAAACACAAGAATTTCAGGCACTTTTAGAGCAGCTTCTACGCA
CTGCCAAGCGCGGAAAGATCCTGCGTGAGGGCTTGTCAACAGCTATTATCGGCCGACCAAATGTAGGAAAGTCTAGTCTA
CTCAATACCTTGTTGCGTGAGGACAAGGCCATTGTCACTGATATTGCAGGAACAACTCGTGATGTCATTGAAGAATATGT
CAATATCAAGGGAATTCCACTAAAGCTAGTTGATACTGCTGGAATTAGAGAAACAGATGATTTAGTAGAGCAAATTGGTG
TTGAGCGCTCTAAAAAAGCCCTACAGGAGGCTGATCTGGTCCTTTTAGTTCTCAATGCTTCGGAAAAGCTGACCGAACAG
GACAAGGCTCTGCTAGCCCTTAGTCAAGATAGCAATCGCATTATTCTCCTCAATAAAACCGATCTGGACCAGGTCATTGA
AAAAGACCAGCTTCCAGAAGAGGCGATCCCAATATCAGTTTTGCAAAACCAAAATATTGACCTGATTGAAGACCGGATCA
ACCAGCTTTTCTTTGATCATACTGGACTAATAGAGCAAGATGCGACTTACCTATCCAATGCTCGTCATATTTCCTTGATT
GAGCAGGCAGTACAAAGCTTAGAGGCTGTCAATGAAGGACTTGCTTTAGGAATGCCGGTTGATCTCTTACAGATTGATTT
GACCAGAGCCTGGGAAATCCTAGGGGAAATCACAGGCGATGCCGCACCTGATGAATTAATCACTCAACTGTTCAGCCAAT
TTTGCCTTGGAAAGTAA

Upstream 100 bases:

>100_bases
ACAACTGCTTTTGAGGCTTTGCCACTAGACAAGAATGACGACAACGAGCAGCTAATGCAATACCTTGCTTTCTATGCTGA
CTTGTTCTACAATAGAAGGC

Downstream 100 bases:

>100_bases
TAGTTCACTGATTTTTTGCCATAACTGCTAGACAAGATCTCTTGATAAGCATGACTGCCTATCAATGTCTCCTCTAAAGC
TAGTTTTACGTCTGTTCTTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSITKEFDTIAAISTPLGEGAIGIVRLSGTKALDIAKSIFKGKDLTTVASHTLNYGHIIKPSTGEVIDEVMVSVMLAPKT
FTREDVIEINTHGGIAVTNDILQLLIKQGARMAEPGEFTKRAFLNGRIDLTQAEAVMDLIRAKTDKAMSIAIKQLDGSLS
QLISDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLRDKTQEFQALLEQLLRTAKRGKILREGLSTAIIGRPNVGKSSL
LNTLLREDKAIVTDIAGTTRDVIEEYVNIKGIPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTEQ
DKALLALSQDSNRIILLNKTDLDQVIEKDQLPEEAIPISVLQNQNIDLIEDRINQLFFDHTGLIEQDATYLSNARHISLI
EQAVQSLEAVNEGLALGMPVDLLQIDLTRAWEILGEITGDAAPDELITQLFSQFCLGK

Sequences:

>Translated_458_residues
MSITKEFDTIAAISTPLGEGAIGIVRLSGTKALDIAKSIFKGKDLTTVASHTLNYGHIIKPSTGEVIDEVMVSVMLAPKT
FTREDVIEINTHGGIAVTNDILQLLIKQGARMAEPGEFTKRAFLNGRIDLTQAEAVMDLIRAKTDKAMSIAIKQLDGSLS
QLISDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLRDKTQEFQALLEQLLRTAKRGKILREGLSTAIIGRPNVGKSSL
LNTLLREDKAIVTDIAGTTRDVIEEYVNIKGIPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTEQ
DKALLALSQDSNRIILLNKTDLDQVIEKDQLPEEAIPISVLQNQNIDLIEDRINQLFFDHTGLIEQDATYLSNARHISLI
EQAVQSLEAVNEGLALGMPVDLLQIDLTRAWEILGEITGDAAPDELITQLFSQFCLGK
>Mature_457_residues
SITKEFDTIAAISTPLGEGAIGIVRLSGTKALDIAKSIFKGKDLTTVASHTLNYGHIIKPSTGEVIDEVMVSVMLAPKTF
TREDVIEINTHGGIAVTNDILQLLIKQGARMAEPGEFTKRAFLNGRIDLTQAEAVMDLIRAKTDKAMSIAIKQLDGSLSQ
LISDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLRDKTQEFQALLEQLLRTAKRGKILREGLSTAIIGRPNVGKSSLL
NTLLREDKAIVTDIAGTTRDVIEEYVNIKGIPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTEQD
KALLALSQDSNRIILLNKTDLDQVIEKDQLPEEAIPISVLQNQNIDLIEDRINQLFFDHTGLIEQDATYLSNARHISLIE
QAVQSLEAVNEGLALGMPVDLLQIDLTRAWEILGEITGDAAPDELITQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=33.6206896551724, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=32.064128256513, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=32.6039387308534, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=34.7826086956522, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082120, Length=119, Percent_Identity=42.0168067226891, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=466, Percent_Identity=30.4721030042918, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=31.5040650406504, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45550813, Length=473, Percent_Identity=30.2325581395349, Blast_Score=204, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR003661
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50386; Mature: 50254

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITKEFDTIAAISTPLGEGAIGIVRLSGTKALDIAKSIFKGKDLTTVASHTLNYGHIIK
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEC
PSTGEVIDEVMVSVMLAPKTFTREDVIEINTHGGIAVTNDILQLLIKQGARMAEPGEFTK
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRIDLTQAEAVMDLIRAKTDKAMSIAIKQLDGSLSQLISDTRQEILNTLAQVEVN
HHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTTALLRDKTQEFQALLEQLLRTAKRGKILREGLSTAIIGRPNVGKSSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHH
LNTLLREDKAIVTDIAGTTRDVIEEYVNIKGIPLKLVDTAGIRETDDLVEQIGVERSKKA
HHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCHHHHHH
LQEADLVLLVLNASEKLTEQDKALLALSQDSNRIILLNKTDLDQVIEKDQLPEEAIPISV
HHHCCEEEEEECCCHHHHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCHHH
LQNQNIDLIEDRINQLFFDHTGLIEQDATYLSNARHISLIEQAVQSLEAVNEGLALGMPV
HCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCH
DLLQIDLTRAWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SITKEFDTIAAISTPLGEGAIGIVRLSGTKALDIAKSIFKGKDLTTVASHTLNYGHIIK
CCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEC
PSTGEVIDEVMVSVMLAPKTFTREDVIEINTHGGIAVTNDILQLLIKQGARMAEPGEFTK
CCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRIDLTQAEAVMDLIRAKTDKAMSIAIKQLDGSLSQLISDTRQEILNTLAQVEVN
HHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTTALLRDKTQEFQALLEQLLRTAKRGKILREGLSTAIIGRPNVGKSSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHH
LNTLLREDKAIVTDIAGTTRDVIEEYVNIKGIPLKLVDTAGIRETDDLVEQIGVERSKKA
HHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCHHHHHH
LQEADLVLLVLNASEKLTEQDKALLALSQDSNRIILLNKTDLDQVIEKDQLPEEAIPISV
HHHCCEEEEEECCCHHHHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHCCCCCCCCHHH
LQNQNIDLIEDRINQLFFDHTGLIEQDATYLSNARHISLIEQAVQSLEAVNEGLALGMPV
HCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCH
DLLQIDLTRAWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]