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The map label for this gene is yhcR [H]

Identifier: 195978209

GI number: 195978209

Start: 1125109

End: 1127115

Strand: Direct

Name: yhcR [H]

Synonym: Sez_1088

Alternate gene names: 195978209

Gene position: 1125109-1127115 (Clockwise)

Preceding gene: 195978201

Following gene: 195978249

Centisome position: 55.58

GC content: 44.25

Gene sequence:

>2007_bases
ATGAAAAAGCATTATGTTTTAAAAAGTAGTGTTCTTGGTGTCTTTGCCGGAGTTTCTATGCTAGTAACCGGCGTGCAGGC
CGATCAGGTAGACGTGCAATTTATTGGTGTCAATGACTTTCATGGTGCCTTAGACACAACCGGCTCTGCCTACATGCCAG
AAGGAAAGGTATCTAACGCTGGTACCGCAGCACAATTAGCTGCTTATCTAGACGATGCAGAGGCTGACTTTAAGCAAACA
AGCCCAGATGGGACAAGCATTCGTGTTCAAGCAGGAGATATGGTCGGAGCTAGTCCTGCTAATTCTGGACTATTACAGGA
CGAGCCAACGGTTAAGGTATTCAACCAGATGGGCTTTGAATACGGAACCCTTGGGAATCACGAATTCGATGAGGGCTTAG
CTGAATTCAACCGTATCATGACAGGCACTGCACCTGCCGAAGACTCAGCTATCAACGACATTACTAAGCAATACAAGCAT
GAAGCTTCAAATCAAACCCTTGTCATTGCAAATGTCGTTGACAAGGAGACCAACACGATTCCCTTTGGCTGGAAGCCTTA
TGCTGTCAAAGAAGTCCCAGTTAATGATAAGTCAGTCAAGGTGGGCTTTATCGGTGTTGTGACAACAGAAATTCCAAACC
TTGTCCTAAAACAAAATTATGAACAATATGATTACCTAGACTCTGCAGAAACCATCGTTAAATATGCTAAAGAGCTCCAA
GAGCAGCAGGTCAATGCGATTGTTGTCCTAGCTCATATCCCAGCAACAAGTGATAACGGGGTAGTGCATGATGAGATGGC
AGCAGTTATGGAAAAGGTTAACCAGCTTTATCCTGATCATAGTGTGGATATTGTCTTTGCTGGTCACAACCACCAATACA
CCAACGGTACCATTGGCACAACACGTGTCGTTCAGGCTGTTTCACAGGGGAAAGCCTATGCAGATGTTCGTGGAACACTA
GACACTGACCTTAATGATTTTGTAGCCACTCCTGAAGCAAAGGTCATTGCCGTTGCTCCTGGCATAAAAACAGGCAATGC
CGACATTCAAGCCATTGTTGATCAAGCCAATGACATTGTCAAAACCGTTACCGAGCTAAAGATCGGTACAGCAGCTACCT
CTTCAACGATTTCTAAAACAGAAAACGAAGACAAGGAATCTCCTGTTGGCAATTTGATAACAACTGCCCAATTAACCATT
GGTAGAAAAACCTTCCCTCATGTTGATTTTGCCATGACAAATAATGGCGGTATTCGCGCTGACTTGGTGGTCAAAGAGGA
TCAAACCATTACCTGGGGAGCTGCACAGGCTGTTCAACCATTTGGCAATATTCTTCAGGTCGTTCAAATGACTGGCCAAC
AAATCTATGATGTCTTGAACCAACAATACGATGAAGACCAAAAATACTTCCTTCAAATGTCTGGACTCAAATACACTTAT
ACAGCTAATGAGGCTAACGATCCTTCAACACCGTTTAAGGTAGTAAAGGCTTATAAAGATAATGGTGAAGAAATTGATCC
AGCAGCTCAATACACCCTAATCATCAACGACTTCCTATATGGCGGTGGTGATGGCTTCTCCGTCTTTAAGGACGCTAAGC
TACTAGGAGCCATTAATCCTGATACTGAGGTCTTTATCGAATACATCAAGGACCTTGAAGCAGCTGGAAAGACTGTAAGT
GCTGGTATTAACGGTGTGAAAACCTATGTCACTGCCAGTCTTGAAGGCTCTACTCAAAGTGACCATACCGGCAAGCACGA
GATCATCAACCGCGTCTTTAAGAATCGTGAGGGCAAGGTGCTTTCTACTGAGCTCGTCTCTGACCTATTTACGCCTGCTC
AAAATGCTAATCCTGCCACAAGCATCAAGCCTCTAGCAGCTAAAAAGCAAGCTAATGCTGCCTTACCAGCAACAGGAACA
AGCCAGCATTCTGCTGCTGCCATGACAATCCTAGGACTACTAACCTTAACTGGTACTAGCCTTTTCAAGAAAAAGGAAGG
CCAATAA

Upstream 100 bases:

>100_bases
TTGAATTTTTGTTAGCTTTTTTATTAATTTTTGTTGAATTCTCATTTAAATAATAGTACACTTAACTTGTGACAATACTT
TAAACTATTTAGGAGCGTTT

Downstream 100 bases:

>100_bases
AACCGGCTTAGCTATAACAGCAATAACCGACTGTATCTTAAGCATACAGCCGGTTTTTTGATTATACGACTTTTAGTTCA
CCTCGCACCTGTGGAACAAG

Product: putative cell surface 5'-nucleotidase

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKKHYVLKSSVLGVFAGVSMLVTGVQADQVDVQFIGVNDFHGALDTTGSAYMPEGKVSNAGTAAQLAAYLDDAEADFKQT
SPDGTSIRVQAGDMVGASPANSGLLQDEPTVKVFNQMGFEYGTLGNHEFDEGLAEFNRIMTGTAPAEDSAINDITKQYKH
EASNQTLVIANVVDKETNTIPFGWKPYAVKEVPVNDKSVKVGFIGVVTTEIPNLVLKQNYEQYDYLDSAETIVKYAKELQ
EQQVNAIVVLAHIPATSDNGVVHDEMAAVMEKVNQLYPDHSVDIVFAGHNHQYTNGTIGTTRVVQAVSQGKAYADVRGTL
DTDLNDFVATPEAKVIAVAPGIKTGNADIQAIVDQANDIVKTVTELKIGTAATSSTISKTENEDKESPVGNLITTAQLTI
GRKTFPHVDFAMTNNGGIRADLVVKEDQTITWGAAQAVQPFGNILQVVQMTGQQIYDVLNQQYDEDQKYFLQMSGLKYTY
TANEANDPSTPFKVVKAYKDNGEEIDPAAQYTLIINDFLYGGGDGFSVFKDAKLLGAINPDTEVFIEYIKDLEAAGKTVS
AGINGVKTYVTASLEGSTQSDHTGKHEIINRVFKNREGKVLSTELVSDLFTPAQNANPATSIKPLAAKKQANAALPATGT
SQHSAAAMTILGLLTLTGTSLFKKKEGQ

Sequences:

>Translated_668_residues
MKKHYVLKSSVLGVFAGVSMLVTGVQADQVDVQFIGVNDFHGALDTTGSAYMPEGKVSNAGTAAQLAAYLDDAEADFKQT
SPDGTSIRVQAGDMVGASPANSGLLQDEPTVKVFNQMGFEYGTLGNHEFDEGLAEFNRIMTGTAPAEDSAINDITKQYKH
EASNQTLVIANVVDKETNTIPFGWKPYAVKEVPVNDKSVKVGFIGVVTTEIPNLVLKQNYEQYDYLDSAETIVKYAKELQ
EQQVNAIVVLAHIPATSDNGVVHDEMAAVMEKVNQLYPDHSVDIVFAGHNHQYTNGTIGTTRVVQAVSQGKAYADVRGTL
DTDLNDFVATPEAKVIAVAPGIKTGNADIQAIVDQANDIVKTVTELKIGTAATSSTISKTENEDKESPVGNLITTAQLTI
GRKTFPHVDFAMTNNGGIRADLVVKEDQTITWGAAQAVQPFGNILQVVQMTGQQIYDVLNQQYDEDQKYFLQMSGLKYTY
TANEANDPSTPFKVVKAYKDNGEEIDPAAQYTLIINDFLYGGGDGFSVFKDAKLLGAINPDTEVFIEYIKDLEAAGKTVS
AGINGVKTYVTASLEGSTQSDHTGKHEIINRVFKNREGKVLSTELVSDLFTPAQNANPATSIKPLAAKKQANAALPATGT
SQHSAAAMTILGLLTLTGTSLFKKKEGQ
>Mature_668_residues
MKKHYVLKSSVLGVFAGVSMLVTGVQADQVDVQFIGVNDFHGALDTTGSAYMPEGKVSNAGTAAQLAAYLDDAEADFKQT
SPDGTSIRVQAGDMVGASPANSGLLQDEPTVKVFNQMGFEYGTLGNHEFDEGLAEFNRIMTGTAPAEDSAINDITKQYKH
EASNQTLVIANVVDKETNTIPFGWKPYAVKEVPVNDKSVKVGFIGVVTTEIPNLVLKQNYEQYDYLDSAETIVKYAKELQ
EQQVNAIVVLAHIPATSDNGVVHDEMAAVMEKVNQLYPDHSVDIVFAGHNHQYTNGTIGTTRVVQAVSQGKAYADVRGTL
DTDLNDFVATPEAKVIAVAPGIKTGNADIQAIVDQANDIVKTVTELKIGTAATSSTISKTENEDKESPVGNLITTAQLTI
GRKTFPHVDFAMTNNGGIRADLVVKEDQTITWGAAQAVQPFGNILQVVQMTGQQIYDVLNQQYDEDQKYFLQMSGLKYTY
TANEANDPSTPFKVVKAYKDNGEEIDPAAQYTLIINDFLYGGGDGFSVFKDAKLLGAINPDTEVFIEYIKDLEAAGKTVS
AGINGVKTYVTASLEGSTQSDHTGKHEIINRVFKNREGKVLSTELVSDLFTPAQNANPATSIKPLAAKKQANAALPATGT
SQHSAAAMTILGLLTLTGTSLFKKKEGQ

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=534, Percent_Identity=23.0337078651685, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1790658, Length=312, Percent_Identity=26.2820512820513, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922444, Length=594, Percent_Identity=22.3905723905724, Blast_Score=77, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19922446, Length=540, Percent_Identity=23.7037037037037, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654424, Length=540, Percent_Identity=23.7037037037037, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 71791; Mature: 71791

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50847 GRAM_POS_ANCHORING ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHYVLKSSVLGVFAGVSMLVTGVQADQVDVQFIGVNDFHGALDTTGSAYMPEGKVSNA
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GTAAQLAAYLDDAEADFKQTSPDGTSIRVQAGDMVGASPANSGLLQDEPTVKVFNQMGFE
CHHHHHHHHHCCCCCCHHCCCCCCCEEEEEECCEECCCCCCCCCCCCCCHHHHHHHCCCC
YGTLGNHEFDEGLAEFNRIMTGTAPAEDSAINDITKQYKHEASNQTLVIANVVDKETNTI
CCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
PFGWKPYAVKEVPVNDKSVKVGFIGVVTTEIPNLVLKQNYEQYDYLDSAETIVKYAKELQ
CCCCCCCEEEECCCCCCEEEEEEEEEEHHHHCHHHHHCCCHHHHHCCHHHHHHHHHHHHH
EQQVNAIVVLAHIPATSDNGVVHDEMAAVMEKVNQLYPDHSVDIVFAGHNHQYTNGTIGT
HCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCCH
TRVVQAVSQGKAYADVRGTLDTDLNDFVATPEAKVIAVAPGIKTGNADIQAIVDQANDIV
HHHHHHHHCCCEEEEECCCCCCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
KTVTELKIGTAATSSTISKTENEDKESPVGNLITTAQLTIGRKTFPHVDFAMTNNGGIRA
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCEEE
DLVVKEDQTITWGAAQAVQPFGNILQVVQMTGQQIYDVLNQQYDEDQKYFLQMSGLKYTY
EEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCEEEEE
TANEANDPSTPFKVVKAYKDNGEEIDPAAQYTLIINDFLYGGGDGFSVFKDAKLLGAINP
ECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHCCCCCCCHHHHCCHHEECCCC
DTEVFIEYIKDLEAAGKTVSAGINGVKTYVTASLEGSTQSDHTGKHEIINRVFKNREGKV
CHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCE
LSTELVSDLFTPAQNANPATSIKPLAAKKQANAALPATGTSQHSAAAMTILGLLTLTGTS
EHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHH
LFKKKEGQ
HHHCCCCC
>Mature Secondary Structure
MKKHYVLKSSVLGVFAGVSMLVTGVQADQVDVQFIGVNDFHGALDTTGSAYMPEGKVSNA
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GTAAQLAAYLDDAEADFKQTSPDGTSIRVQAGDMVGASPANSGLLQDEPTVKVFNQMGFE
CHHHHHHHHHCCCCCCHHCCCCCCCEEEEEECCEECCCCCCCCCCCCCCHHHHHHHCCCC
YGTLGNHEFDEGLAEFNRIMTGTAPAEDSAINDITKQYKHEASNQTLVIANVVDKETNTI
CCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
PFGWKPYAVKEVPVNDKSVKVGFIGVVTTEIPNLVLKQNYEQYDYLDSAETIVKYAKELQ
CCCCCCCEEEECCCCCCEEEEEEEEEEHHHHCHHHHHCCCHHHHHCCHHHHHHHHHHHHH
EQQVNAIVVLAHIPATSDNGVVHDEMAAVMEKVNQLYPDHSVDIVFAGHNHQYTNGTIGT
HCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCCH
TRVVQAVSQGKAYADVRGTLDTDLNDFVATPEAKVIAVAPGIKTGNADIQAIVDQANDIV
HHHHHHHHCCCEEEEECCCCCCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
KTVTELKIGTAATSSTISKTENEDKESPVGNLITTAQLTIGRKTFPHVDFAMTNNGGIRA
HHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCEEE
DLVVKEDQTITWGAAQAVQPFGNILQVVQMTGQQIYDVLNQQYDEDQKYFLQMSGLKYTY
EEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCEEEEE
TANEANDPSTPFKVVKAYKDNGEEIDPAAQYTLIINDFLYGGGDGFSVFKDAKLLGAINP
ECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEHHHCCCCCCCHHHHCCHHEECCCC
DTEVFIEYIKDLEAAGKTVSAGINGVKTYVTASLEGSTQSDHTGKHEIINRVFKNREGKV
CHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCE
LSTELVSDLFTPAQNANPATSIKPLAAKKQANAALPATGTSQHSAAAMTILGLLTLTGTS
EHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHH
LFKKKEGQ
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]