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The map label for this gene is artJ [H]

Identifier: 195978015

GI number: 195978015

Start: 931644

End: 932480

Strand: Direct

Name: artJ [H]

Synonym: Sez_0888

Alternate gene names: 195978015

Gene position: 931644-932480 (Clockwise)

Preceding gene: 195978014

Following gene: 195978018

Centisome position: 46.03

GC content: 39.67

Gene sequence:

>837_bases
ATGACGTTAAAAAAGACCCTTCTAGTAGCCCTATTTGCTGCTACAGCTACGCTTTTAGTAGCTTGCGGCGCTAGTAATAA
ACAGACTAGCTCAGATGCTTGGGATTCCTACAAGCAGAAAAAGACGATTACGCTTGGCTTTGATAATACTTTTGTGCCAA
TGGGCTATAAGGACGAGTCAGGAAAAAATACCGGCTTTGATATTGAGCTAGCAAAGGCTGTTTTTGACCAATATGGTATC
AAGGTTAAGTATCAAGCCATTAACTGGGATCTCAAGGAAACTGAGTTGAACAATGGTAAAATTGACCTGATTTGGAATGG
CTATTCTGTGACAAAAGAGCGCCAAGCTAAGGTGGCCTTTACAGAGCCTTATATGAAAAATGAGCAGGTCTTAGTGACTC
AAAAATCATCACAGATAAGCTCATTTGCTGATATGAAAAATAAAACCTTGGGTGCTCAGTCTGGCTCATCTGGCTATGAT
GCCTTTACAAGTCATCCTAAGGTGCTAAAGGATCTTGTCAAAGGAAATGATGCTACCCAATATGAAACCTTTACACAAGC
CTTTATCGACTTAAAAAACAAGCGTATTGATGGCCTTTTGATTGATAGGGTTTATGCCAATTATTACCTTCAGCAAGAAG
GTGAGCTAGACCGCTATAACATTATCGCGAGTGAGTTTGAAGGTGAGGATTTTGCAGTTGGTGTCCGAAAAGCGGATAAA
ACCCTACAAAAAAAGATTACACAAGCCTTAAAATCGCTTTATCAGGACGGTACCTTCCAAAAAATCTCTAAAAAATGGTT
TGGTGAAGATGTCGCAACAGTTCAGTTGAAATCATAA

Upstream 100 bases:

>100_bases
GGCATTACCCAGATTGTTGTCACCCATGATTTGCAGTTTGCTCAGACCATTTCTGATCGTATCATCAAGATCAATCCTAA
ATAGAAGAAGAGGTAGTGCT

Downstream 100 bases:

>100_bases
GACGCAAGCAAGACCTAATGAGCTTAGAAGGCAGAATGGCTGGATAGAAAAAAACTTATCCTATCAGGGTAAGTTTTTTG
TTTGCTTGTGACTGATGATA

Product: amino acid ABC transporter extracellular-binding protein precursor

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTLKKTLLVALFAATATLLVACGASNKQTSSDAWDSYKQKKTITLGFDNTFVPMGYKDESGKNTGFDIELAKAVFDQYGI
KVKYQAINWDLKETELNNGKIDLIWNGYSVTKERQAKVAFTEPYMKNEQVLVTQKSSQISSFADMKNKTLGAQSGSSGYD
AFTSHPKVLKDLVKGNDATQYETFTQAFIDLKNKRIDGLLIDRVYANYYLQQEGELDRYNIIASEFEGEDFAVGVRKADK
TLQKKITQALKSLYQDGTFQKISKKWFGEDVATVQLKS

Sequences:

>Translated_278_residues
MTLKKTLLVALFAATATLLVACGASNKQTSSDAWDSYKQKKTITLGFDNTFVPMGYKDESGKNTGFDIELAKAVFDQYGI
KVKYQAINWDLKETELNNGKIDLIWNGYSVTKERQAKVAFTEPYMKNEQVLVTQKSSQISSFADMKNKTLGAQSGSSGYD
AFTSHPKVLKDLVKGNDATQYETFTQAFIDLKNKRIDGLLIDRVYANYYLQQEGELDRYNIIASEFEGEDFAVGVRKADK
TLQKKITQALKSLYQDGTFQKISKKWFGEDVATVQLKS
>Mature_277_residues
TLKKTLLVALFAATATLLVACGASNKQTSSDAWDSYKQKKTITLGFDNTFVPMGYKDESGKNTGFDIELAKAVFDQYGIK
VKYQAINWDLKETELNNGKIDLIWNGYSVTKERQAKVAFTEPYMKNEQVLVTQKSSQISSFADMKNKTLGAQSGSSGYDA
FTSHPKVLKDLVKGNDATQYETFTQAFIDLKNKRIDGLLIDRVYANYYLQQEGELDRYNIIASEFEGEDFAVGVRKADKT
LQKKITQALKSLYQDGTFQKISKKWFGEDVATVQLKS

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=31.8777292576419, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1788228, Length=264, Percent_Identity=28.030303030303, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787088, Length=267, Percent_Identity=26.2172284644195, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787031, Length=267, Percent_Identity=24.3445692883895, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788649, Length=233, Percent_Identity=30.4721030042918, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788648, Length=240, Percent_Identity=28.75, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31251; Mature: 31119

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKKTLLVALFAATATLLVACGASNKQTSSDAWDSYKQKKTITLGFDNTFVPMGYKDES
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEECCCEEECCCCCCCC
GKNTGFDIELAKAVFDQYGIKVKYQAINWDLKETELNNGKIDLIWNGYSVTKERQAKVAF
CCCCCCCHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCHHCCEEEE
TEPYMKNEQVLVTQKSSQISSFADMKNKTLGAQSGSSGYDAFTSHPKVLKDLVKGNDATQ
ECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHHHHHHCCCCCHH
YETFTQAFIDLKNKRIDGLLIDRVYANYYLQQEGELDRYNIIASEFEGEDFAVGVRKADK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHEEEHHHCCCCCEEEEHHHHHH
TLQKKITQALKSLYQDGTFQKISKKWFGEDVATVQLKS
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TLKKTLLVALFAATATLLVACGASNKQTSSDAWDSYKQKKTITLGFDNTFVPMGYKDES
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEEECCCEEECCCCCCCC
GKNTGFDIELAKAVFDQYGIKVKYQAINWDLKETELNNGKIDLIWNGYSVTKERQAKVAF
CCCCCCCHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCHHCCEEEE
TEPYMKNEQVLVTQKSSQISSFADMKNKTLGAQSGSSGYDAFTSHPKVLKDLVKGNDATQ
ECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHHHHHHCCCCCHH
YETFTQAFIDLKNKRIDGLLIDRVYANYYLQQEGELDRYNIIASEFEGEDFAVGVRKADK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHEEEHHHCCCCCEEEEHHHHHH
TLQKKITQALKSLYQDGTFQKISKKWFGEDVATVQLKS
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]