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The map label for this gene is pcrA [H]

Identifier: 195978018

GI number: 195978018

Start: 935746

End: 938043

Strand: Direct

Name: pcrA [H]

Synonym: Sez_0891

Alternate gene names: 195978018

Gene position: 935746-938043 (Clockwise)

Preceding gene: 195978015

Following gene: 195978019

Centisome position: 46.23

GC content: 44.47

Gene sequence:

>2298_bases
ATGAATCCTTTATTAGATGGAATGAATGATAAACAGGCAGAGGCAGTTCAAACGACTGAGGGCCCCTTGTTAATTATGGC
TGGTGCCGGTTCTGGGAAAACGAGGGTATTAACCCATCGCATTGCTTACTTGATTGATGAAAAATATGTTAATCCCTGGA
ATATTTTGGCGATTACCTTTACCAACAAGGCTGCGCGTGAGATGCGAGAAAGGGCCTTGGCCTTAAGCCCAGCCACCAAG
GATACCTTGATTGCGACCTTTCACTCAATGTGTGTTCGTATTTTACGCCGTGAGGCAGATCATATTGGCTACAATCGGAA
TTTCACGATTGTTGATCCAGGAGAACAGCGGACCTTAATGAAGAGAATAGTAAAAGCTCTAAATCTGGATCCTAAGAAAT
GGAGCGAGCGGTCGATTTTAGCCACTATTTCAAATGCTAAAAATGATCTCTTAGATGAAAGAGCCTATGAGCTAGGAGCC
TCTGATTTGTATAGCCAGACAGTTGCCCGCTGCTACAAGGCCTATCAGGAGGAGCTCAGACGAAGTGAGGCGATGGATTT
TGATGATTTGATTATGATGACCCTGCGCTTGTTTGATCAAAATCCTGACGTTCTAGCCTATTATCAGCAGCGTTATCAAT
ATATACATGTCGATGAATACCAAGACACAAACCACGCTCAATATCAGCTAGTGACTCTTTTAGCCTCACGCTTTAAAAAT
ATCTGTGTTGTCGGTGATGCAGACCAGTCCATTTATGGCTGGCGTGGTGCTGATATGCAAAATATTTTAGATTTTGAAAA
GGATTACCCTGATGCAAGGGTGGTTTTGCTGGAAGAAAATTATCGCTCCACCAAGAAAATCCTTCAGGCGGCTAACGAAG
TGATTCAGCATAATCGGCATCGCCGTCCGAAAAAACTGTGGACTCAAAACGCAGACGGTGAGCAGATTGTTTATTATAGG
GCAAATGATGAGCGTGACGAGGCTGTTTTTGTCGCCTCAACCATCAGCAATATGTGTCTAGAGTTAGGAAAGAGCTTTAA
AGACTTTGCTGTTTTGTATCGTACCAATGCTCAGTCCCGTACAATTGAAGAAGCCCTGTTAAAATCAAATATCCCTTACA
CAATGGTCGGAGGAACCAAATTCTATAGCAGAAAGGAAATTCGTGATGTGATTGCCTATTTGACTGTAGTTGCTAATCCG
TCAGATAATATCTCCTTTGAACGAATTGTGAATGAGCCTAAACGAGGGGTAGGACCGGGAACCTTGGATAAGCTCCGCCA
ATTTGCTTATGGGCAAGAGCAGTCATTGTTAGAGGCCGCTTCAAGCCTTGAGCAATCACCGCTAAAAGGAAAGGCTGCCC
AAGCCATGCTGGCCTTAGCGAGTCTCTTAAGTGATTTGAGAGCTGATTTGGACCGGCTGAGCATTACAGCCTTAGCCGAG
GCCTTACTGGAGAAAAGCGGCTACCTGGATATGCTGCGTGTTCAAAATACCTTAGAAAGTCAAGCTAGGATTGAAAATAT
TGAAGAATTCCTGTCTGTTACCAAAAGCTTTGATGACGTCTCAGCGCAGCAGCAGGAGCATGAAGCAGGCATTGACCGCT
TAGGAAGGTTTCTCAATGATCTTGCTTTGATTGCTGATAGTGATGATGGCAATGCAGAGACTGCTGAGGTAACTCTGATG
ACCCTGCATGCAGCTAAGGGGCTAGAATTTCCTGTTGTTTTTCTCATAGGTATGGAGGAGGGAGTCTTTCCTTTAGCTCG
AGCAGCCGAGGACCAAGATGAGCTAGAAGAAGAAAGGCGACTAGCCTATGTCGGCATTACCAGAGCAGAGGAATGCTTGT
TTTTAACAAATGCCAATACACGTACCCTATTTGGTAAGAGCAGCTATAATCGCCCAACAAGGTTTTTAAAAGAGATGTCA
GAGAACCTTTTAAGCTTCCAAGGCTTGGCAAGACCTGCTCATGCTTCCTTTGGTGTGACATTTTCGCAGCAGGGTCAGCG
ACAATTTTGCGCAGGAATGAGCCTATCAGAGGCTATTCAGTCTAGAAAGTCAATGGCACAGCCTGCCAGAGCAGCCTCAA
AGCCTACACCGTTGCCATTTGGACCAAATGCAGCTACCTCAAAGCAAGCCATTGATTGGCAGATTGGTGATATTGCTCAT
CATAAAAAATGGGGTGCTGGAACAGTTTTAGAGGTCAAGGGCTCTGGTAAGACCATGGAATTAACCATTAGCTTTCCTGA
TGTTGGTACTAAAAAAACTCCTCGCCAGCGTTGCTCCTATCGAAAAAAAATAATATAG

Upstream 100 bases:

>100_bases
TTTATGGTAACATCACTGTCCTCTTTTTTCTTTTTGACTGAAACATGATATAATCTTAAGGTTAATAAAAGTCACTTATT
GGACGATATGAGGAGAAAGC

Downstream 100 bases:

>100_bases
CTTATGGGCAAGCTTGCATAGTCATCTGGACGAGGGCTTTGCCAGTACTGTTTGATTCTATGATTTAAAAAAAGAAGTGA
ACAAAGATTTTATCAGTTAA

Product: ATP-dependent DNA helicase UvrD/PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MNPLLDGMNDKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKYVNPWNILAITFTNKAAREMRERALALSPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRIVKALNLDPKKWSERSILATISNAKNDLLDERAYELGA
SDLYSQTVARCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVTLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDARVVLLEENYRSTKKILQAANEVIQHNRHRRPKKLWTQNADGEQIVYYR
ANDERDEAVFVASTISNMCLELGKSFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIAYLTVVANP
SDNISFERIVNEPKRGVGPGTLDKLRQFAYGQEQSLLEAASSLEQSPLKGKAAQAMLALASLLSDLRADLDRLSITALAE
ALLEKSGYLDMLRVQNTLESQARIENIEEFLSVTKSFDDVSAQQQEHEAGIDRLGRFLNDLALIADSDDGNAETAEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLARAAEDQDELEEERRLAYVGITRAEECLFLTNANTRTLFGKSSYNRPTRFLKEMS
ENLLSFQGLARPAHASFGVTFSQQGQRQFCAGMSLSEAIQSRKSMAQPARAASKPTPLPFGPNAATSKQAIDWQIGDIAH
HKKWGAGTVLEVKGSGKTMELTISFPDVGTKKTPRQRCSYRKKII

Sequences:

>Translated_765_residues
MNPLLDGMNDKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKYVNPWNILAITFTNKAAREMRERALALSPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRIVKALNLDPKKWSERSILATISNAKNDLLDERAYELGA
SDLYSQTVARCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVTLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDARVVLLEENYRSTKKILQAANEVIQHNRHRRPKKLWTQNADGEQIVYYR
ANDERDEAVFVASTISNMCLELGKSFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIAYLTVVANP
SDNISFERIVNEPKRGVGPGTLDKLRQFAYGQEQSLLEAASSLEQSPLKGKAAQAMLALASLLSDLRADLDRLSITALAE
ALLEKSGYLDMLRVQNTLESQARIENIEEFLSVTKSFDDVSAQQQEHEAGIDRLGRFLNDLALIADSDDGNAETAEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLARAAEDQDELEEERRLAYVGITRAEECLFLTNANTRTLFGKSSYNRPTRFLKEMS
ENLLSFQGLARPAHASFGVTFSQQGQRQFCAGMSLSEAIQSRKSMAQPARAASKPTPLPFGPNAATSKQAIDWQIGDIAH
HKKWGAGTVLEVKGSGKTMELTISFPDVGTKKTPRQRCSYRKKII
>Mature_765_residues
MNPLLDGMNDKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKYVNPWNILAITFTNKAAREMRERALALSPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRIVKALNLDPKKWSERSILATISNAKNDLLDERAYELGA
SDLYSQTVARCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVTLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDARVVLLEENYRSTKKILQAANEVIQHNRHRRPKKLWTQNADGEQIVYYR
ANDERDEAVFVASTISNMCLELGKSFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIAYLTVVANP
SDNISFERIVNEPKRGVGPGTLDKLRQFAYGQEQSLLEAASSLEQSPLKGKAAQAMLALASLLSDLRADLDRLSITALAE
ALLEKSGYLDMLRVQNTLESQARIENIEEFLSVTKSFDDVSAQQQEHEAGIDRLGRFLNDLALIADSDDGNAETAEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLARAAEDQDELEEERRLAYVGITRAEECLFLTNANTRTLFGKSSYNRPTRFLKEMS
ENLLSFQGLARPAHASFGVTFSQQGQRQFCAGMSLSEAIQSRKSMAQPARAASKPTPLPFGPNAATSKQAIDWQIGDIAH
HKKWGAGTVLEVKGSGKTMELTISFPDVGTKKTPRQRCSYRKKII

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=755, Percent_Identity=39.3377483443709, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI48994965, Length=648, Percent_Identity=38.5802469135802, Blast_Score=411, Evalue=1e-115,
Organism=Escherichia coli, GI1787196, Length=339, Percent_Identity=26.8436578171091, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=738, Percent_Identity=28.8617886178862, Blast_Score=233, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6324477, Length=710, Percent_Identity=23.0985915492958, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86186; Mature: 86186

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLDGMNDKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKYVNPWNILAITF
CCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
TNKAAREMRERALALSPATKDTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLM
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
KRIVKALNLDPKKWSERSILATISNAKNDLLDERAYELGASDLYSQTVARCYKAYQEELR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RSEAMDFDDLIMMTLRLFDQNPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVTLLASRFKN
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCC
ICVVGDADQSIYGWRGADMQNILDFEKDYPDARVVLLEENYRSTKKILQAANEVIQHNRH
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
RRPKKLWTQNADGEQIVYYRANDERDEAVFVASTISNMCLELGKSFKDFAVLYRTNAQSR
CCCHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHEECCCCHH
TIEEALLKSNIPYTMVGGTKFYSRKEIRDVIAYLTVVANPSDNISFERIVNEPKRGVGPG
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCC
TLDKLRQFAYGQEQSLLEAASSLEQSPLKGKAAQAMLALASLLSDLRADLDRLSITALAE
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLEKSGYLDMLRVQNTLESQARIENIEEFLSVTKSFDDVSAQQQEHEAGIDRLGRFLND
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALIADSDDGNAETAEVTLMTLHAAKGLEFPVVFLIGMEEGVFPLARAAEDQDELEEERR
HEEEEECCCCCCCCCEEEEEEEEHHCCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHC
LAYVGITRAEECLFLTNANTRTLFGKSSYNRPTRFLKEMSENLLSFQGLARPAHASFGVT
EEEEECCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
FSQQGQRQFCAGMSLSEAIQSRKSMAQPARAASKPTPLPFGPNAATSKQAIDWQIGDIAH
ECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHH
HKKWGAGTVLEVKGSGKTMELTISFPDVGTKKTPRQRCSYRKKII
CCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNPLLDGMNDKQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKYVNPWNILAITF
CCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
TNKAAREMRERALALSPATKDTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLM
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
KRIVKALNLDPKKWSERSILATISNAKNDLLDERAYELGASDLYSQTVARCYKAYQEELR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RSEAMDFDDLIMMTLRLFDQNPDVLAYYQQRYQYIHVDEYQDTNHAQYQLVTLLASRFKN
HHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHCC
ICVVGDADQSIYGWRGADMQNILDFEKDYPDARVVLLEENYRSTKKILQAANEVIQHNRH
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
RRPKKLWTQNADGEQIVYYRANDERDEAVFVASTISNMCLELGKSFKDFAVLYRTNAQSR
CCCHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHEECCCCHH
TIEEALLKSNIPYTMVGGTKFYSRKEIRDVIAYLTVVANPSDNISFERIVNEPKRGVGPG
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCC
TLDKLRQFAYGQEQSLLEAASSLEQSPLKGKAAQAMLALASLLSDLRADLDRLSITALAE
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLEKSGYLDMLRVQNTLESQARIENIEEFLSVTKSFDDVSAQQQEHEAGIDRLGRFLND
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALIADSDDGNAETAEVTLMTLHAAKGLEFPVVFLIGMEEGVFPLARAAEDQDELEEERR
HEEEEECCCCCCCCCEEEEEEEEHHCCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHC
LAYVGITRAEECLFLTNANTRTLFGKSSYNRPTRFLKEMSENLLSFQGLARPAHASFGVT
EEEEECCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
FSQQGQRQFCAGMSLSEAIQSRKSMAQPARAASKPTPLPFGPNAATSKQAIDWQIGDIAH
ECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHH
HKKWGAGTVLEVKGSGKTMELTISFPDVGTKKTPRQRCSYRKKII
CCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA