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The map label for this gene is iscS [C]

Identifier: 195977997

GI number: 195977997

Start: 911320

End: 912462

Strand: Direct

Name: iscS [C]

Synonym: Sez_0869

Alternate gene names: 195977997

Gene position: 911320-912462 (Clockwise)

Preceding gene: 195977995

Following gene: 195977998

Centisome position: 45.02

GC content: 43.31

Gene sequence:

>1143_bases
ATGATTTATTTTGATAATGCAGCCACTACCAAGCCTTACGAAGAGGCACTTAAAACGTATCAGGAAGTGGCCAGTAAGAT
TTACGGCAATCCCTCTAGTCTCCATCAGCTAGGAGCCAATGCCAGTCGTATATTGGAGGCTTCGCGCAGGCAAATAGCGG
CCTTACTTGATGTAGAGGCCAAAGAGATTTTCTTTACCTCTGGCGGAACGGAAAGCGACAATTGGGCTATCAAGGGGATT
GCCTTTGAAAAATCTCACTTTGGTAAGCACATCATTGTCTCAGCCATTGAGCACCCAGCTGTCAGAGAAAGCGCCAAATG
GCTAGAAAGTCAAGGCTTTGAGCTGTCTTATGCTCCTGTCACAGATAAGGGAGTTGTTGATGTCAAAAAGCTAGCTGCCT
TAATCCGAAAAGATACCATATTAGTTTCCATTATGGCGGTTAACAATGAGATTGGGTCAATTCAGCCAATTGCAGCCATA
TCAGAGCTTTTGGCTGATAAGCCCACCATTACCTTTCATGTCGATGCTGTTCAGGCTATTGGGAAGCTTCCTGTTGAGGC
TTACTTAACCGCGCGTGTTGACTTAGCTTCTTTTTCTGGTCATAAATTTCATGCTGTTCGTGGTGTTGGCTTTCTTTATA
AAAAATCAGGAAAGCGAATCACTCCCTTATTGGTCGGTGGCGGTCAAGAAGATGAGCTACGTTCGACAACTGAAAACGTG
GCAGCTATTGCAAGTATGGCCAAGGCTTTAAGGATCATCACAGAAAGACAGGATAGATCTCAAGCAAGGATAGCAGCCAT
GAAAAAGGTTATTTATCAAGCCTTGCAGGCCTATGAGGATACCATTCTCTTTTCTGAGATAGATGATGTTGCTCCTCATA
TTCTGACCTTTGGTATAAAGGGCGTTCGTGGAGAGGTCATTGTGCACGCATTTGAAAAGCATGAGATTTACATATCAACA
ACGAGTGCCTGCTCATCAAAGGCTGGAAAGCCAGCAGGAACCTTAGTCTCAATGGGAATTCCAGCAAAATTGGCTCAAAC
AGCAGTCCGTATCAGCCTAGATGATGATAATGACATGGGACAGGTTGAGCAATTTTTAACGATTTTTAAACAGATTTATG
AAAAAACACAAAAGGTTAGATAA

Upstream 100 bases:

>100_bases
TTGTCTAGGATAGAGTTGTCCTATCCTGCAAAAAGATCTATTTTTTCATTTCATCAAATTTGTGATACAATAAAGTATTG
AATAAAAATGAAGGTGACAC

Downstream 100 bases:

>100_bases
TATGAAATATTCAGAAATAATGGTTCGTCATGGGGAGTTATCGACCAAAGGAAAAAACCGTATGCGGTTTATTAATCGGC
TAAAAGCTAATATTCAAGAT

Product: cysteine desulfurase IscS

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIYFDNAATTKPYEEALKTYQEVASKIYGNPSSLHQLGANASRILEASRRQIAALLDVEAKEIFFTSGGTESDNWAIKGI
AFEKSHFGKHIIVSAIEHPAVRESAKWLESQGFELSYAPVTDKGVVDVKKLAALIRKDTILVSIMAVNNEIGSIQPIAAI
SELLADKPTITFHVDAVQAIGKLPVEAYLTARVDLASFSGHKFHAVRGVGFLYKKSGKRITPLLVGGGQEDELRSTTENV
AAIASMAKALRIITERQDRSQARIAAMKKVIYQALQAYEDTILFSEIDDVAPHILTFGIKGVRGEVIVHAFEKHEIYIST
TSACSSKAGKPAGTLVSMGIPAKLAQTAVRISLDDDNDMGQVEQFLTIFKQIYEKTQKVR

Sequences:

>Translated_380_residues
MIYFDNAATTKPYEEALKTYQEVASKIYGNPSSLHQLGANASRILEASRRQIAALLDVEAKEIFFTSGGTESDNWAIKGI
AFEKSHFGKHIIVSAIEHPAVRESAKWLESQGFELSYAPVTDKGVVDVKKLAALIRKDTILVSIMAVNNEIGSIQPIAAI
SELLADKPTITFHVDAVQAIGKLPVEAYLTARVDLASFSGHKFHAVRGVGFLYKKSGKRITPLLVGGGQEDELRSTTENV
AAIASMAKALRIITERQDRSQARIAAMKKVIYQALQAYEDTILFSEIDDVAPHILTFGIKGVRGEVIVHAFEKHEIYIST
TSACSSKAGKPAGTLVSMGIPAKLAQTAVRISLDDDNDMGQVEQFLTIFKQIYEKTQKVR
>Mature_380_residues
MIYFDNAATTKPYEEALKTYQEVASKIYGNPSSLHQLGANASRILEASRRQIAALLDVEAKEIFFTSGGTESDNWAIKGI
AFEKSHFGKHIIVSAIEHPAVRESAKWLESQGFELSYAPVTDKGVVDVKKLAALIRKDTILVSIMAVNNEIGSIQPIAAI
SELLADKPTITFHVDAVQAIGKLPVEAYLTARVDLASFSGHKFHAVRGVGFLYKKSGKRITPLLVGGGQEDELRSTTENV
AAIASMAKALRIITERQDRSQARIAAMKKVIYQALQAYEDTILFSEIDDVAPHILTFGIKGVRGEVIVHAFEKHEIYIST
TSACSSKAGKPAGTLVSMGIPAKLAQTAVRISLDDDNDMGQVEQFLTIFKQIYEKTQKVR

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=356, Percent_Identity=33.9887640449438, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI156713448, Length=407, Percent_Identity=30.7125307125307, Blast_Score=166, Evalue=2e-41,
Organism=Escherichia coli, GI48994898, Length=358, Percent_Identity=40.2234636871508, Blast_Score=240, Evalue=9e-65,
Organism=Escherichia coli, GI1789175, Length=278, Percent_Identity=28.4172661870504, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787970, Length=232, Percent_Identity=28.0172413793103, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=369, Percent_Identity=34.4173441734417, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17533177, Length=326, Percent_Identity=30.0613496932515, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=355, Percent_Identity=36.6197183098592, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20129463, Length=356, Percent_Identity=33.1460674157303, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 41551; Mature: 41551

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNAATTKPYEEALKTYQEVASKIYGNPSSLHQLGANASRILEASRRQIAALLDVEA
CEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
KEIFFTSGGTESDNWAIKGIAFEKSHFGKHIIVSAIEHPAVRESAKWLESQGFELSYAPV
CEEEEECCCCCCCCEEEEEEEEEHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCEEEECCC
TDKGVVDVKKLAALIRKDTILVSIMAVNNEIGSIQPIAAISELLADKPTITFHVDAVQAI
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHH
GKLPVEAYLTARVDLASFSGHKFHAVRGVGFLYKKSGKRITPLLVGGGQEDELRSTTENV
HCCCHHHHHEEEEEEECCCCCEEEEECCCHHEEECCCCEEEEEEECCCCCHHHHHHHHHH
AAIASMAKALRIITERQDRSQARIAAMKKVIYQALQAYEDTILFSEIDDVAPHILTFGIK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GVRGEVIVHAFEKHEIYISTTSACSSKAGKPAGTLVSMGIPAKLAQTAVRISLDDDNDMG
CCCCEEEEEEEECCEEEEEECCHHHCCCCCCCCCEEECCCCHHHHHHEEEEEECCCCCHH
QVEQFLTIFKQIYEKTQKVR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYFDNAATTKPYEEALKTYQEVASKIYGNPSSLHQLGANASRILEASRRQIAALLDVEA
CEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
KEIFFTSGGTESDNWAIKGIAFEKSHFGKHIIVSAIEHPAVRESAKWLESQGFELSYAPV
CEEEEECCCCCCCCEEEEEEEEEHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCEEEECCC
TDKGVVDVKKLAALIRKDTILVSIMAVNNEIGSIQPIAAISELLADKPTITFHVDAVQAI
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHH
GKLPVEAYLTARVDLASFSGHKFHAVRGVGFLYKKSGKRITPLLVGGGQEDELRSTTENV
HCCCHHHHHEEEEEEECCCCCEEEEECCCHHEEECCCCEEEEEEECCCCCHHHHHHHHHH
AAIASMAKALRIITERQDRSQARIAAMKKVIYQALQAYEDTILFSEIDDVAPHILTFGIK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GVRGEVIVHAFEKHEIYISTTSACSSKAGKPAGTLVSMGIPAKLAQTAVRISLDDDNDMG
CCCCEEEEEEEECCEEEEEECCHHHCCCCCCCCCEEECCCCHHHHHHEEEEEECCCCCHH
QVEQFLTIFKQIYEKTQKVR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]