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The map label for this gene is levE [H]

Identifier: 195977874

GI number: 195977874

Start: 775668

End: 776153

Strand: Direct

Name: levE [H]

Synonym: Sez_0742

Alternate gene names: 195977874

Gene position: 775668-776153 (Clockwise)

Preceding gene: 195977873

Following gene: 195977875

Centisome position: 38.32

GC content: 43.62

Gene sequence:

>486_bases
ATGACAATTGTTGCAGCACGTATTGACGGCCGGTTAATTCATGGTCAGGTAGCTAATCTATGGACGACAAAGCTAAACAT
TAGCCGTATCATGGTGGTCGATAATGAGGTGATTAGCAATGACCTTGAAAAAACAGCACTGAAGCTAGCCACGCCTAGCG
GTGTTAAATTATCTATCTTATCCGTTGAAAAAGCAGCCAACAATATTTTGGAAGGGCGCTATGATTCTCAACGCTTGTTG
ATAGTAGCCAGAAAGCCGGATAGCTTTTTGGGGCTGATTCAAAAAGGGGTTGCTATTTCAGAGTTAAATGTCGGCAATAT
GTCACAGACACCGGAGACTCGTCCAGTAACGCGTTCTATTAACGTTGTAGATGAGGATATAGAGGCATTCAAGGCTATTT
CTGCCAAGGGGACTCATCTCATTGCACAAATGGTCCCAAGTGATGCCCCAGCAGACTTTATGCCGCTTTTGGATAAGGTT
GATTAA

Upstream 100 bases:

>100_bases
GTCAAAATAGACTGCTGATATTTGAAACCGAAGGGCAGTTTAGTGAGTCAATTCGTTTAACTAAGGAACCAATTTATAAT
GATATAAAAGGAGAAAACTT

Downstream 100 bases:

>100_bases
GATGTGTCAAATCAAAAACGAATCTGTTTCAGGAGGTAAAAGCGTATGATTCAATGGTGGCAAATCCTATTATTAACACT
CTACTCAGCTTATCAAATAT

Product: PTS system mannose/fructose IIB component

Products: NA

Alternate protein names: EIIB-Fru; PTS system fructose-specific EIIB component; p18 [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTIVAARIDGRLIHGQVANLWTTKLNISRIMVVDNEVISNDLEKTALKLATPSGVKLSILSVEKAANNILEGRYDSQRLL
IVARKPDSFLGLIQKGVAISELNVGNMSQTPETRPVTRSINVVDEDIEAFKAISAKGTHLIAQMVPSDAPADFMPLLDKV
D

Sequences:

>Translated_161_residues
MTIVAARIDGRLIHGQVANLWTTKLNISRIMVVDNEVISNDLEKTALKLATPSGVKLSILSVEKAANNILEGRYDSQRLL
IVARKPDSFLGLIQKGVAISELNVGNMSQTPETRPVTRSINVVDEDIEAFKAISAKGTHLIAQMVPSDAPADFMPLLDKV
D
>Mature_160_residues
TIVAARIDGRLIHGQVANLWTTKLNISRIMVVDNEVISNDLEKTALKLATPSGVKLSILSVEKAANNILEGRYDSQRLLI
VARKPDSFLGLIQKGVAISELNVGNMSQTPETRPVTRSINVVDEDIEAFKAISAKGTHLIAQMVPSDAPADFMPLLDKVD

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=161, Percent_Identity=37.2670807453416, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI87082217, Length=155, Percent_Identity=30.3225806451613, Blast_Score=92, Evalue=1e-20,
Organism=Escherichia coli, GI1789527, Length=147, Percent_Identity=27.891156462585, Blast_Score=70, Evalue=6e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17503; Mature: 17371

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVAARIDGRLIHGQVANLWTTKLNISRIMVVDNEVISNDLEKTALKLATPSGVKLSIL
CEEEEEECCCEEEECCHHHEEEEEECEEEEEEECCHHHHHHHHHHHEEEECCCCCEEEEE
SVEKAANNILEGRYDSQRLLIVARKPDSFLGLIQKGVAISELNVGNMSQTPETRPVTRSI
EHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEC
NVVDEDIEAFKAISAKGTHLIAQMVPSDAPADFMPLLDKVD
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TIVAARIDGRLIHGQVANLWTTKLNISRIMVVDNEVISNDLEKTALKLATPSGVKLSIL
EEEEEECCCEEEECCHHHEEEEEECEEEEEEECCHHHHHHHHHHHEEEECCCCCEEEEE
SVEKAANNILEGRYDSQRLLIVARKPDSFLGLIQKGVAISELNVGNMSQTPETRPVTRSI
EHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEC
NVVDEDIEAFKAISAKGTHLIAQMVPSDAPADFMPLLDKVD
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377; 9551099 [H]