Click here to switch to the map view.

The map label for this gene is metK [H]

Identifier: 195977814

GI number: 195977814

Start: 705644

End: 706849

Strand: Direct

Name: metK [H]

Synonym: Sez_0680

Alternate gene names: 195977814

Gene position: 705644-706849 (Clockwise)

Preceding gene: 195977813

Following gene: 195977815

Centisome position: 34.86

GC content: 46.6

Gene sequence:

>1206_bases
ATGTCAGAACGTAAGCTTTTCACGTCTGAATCAGTATCTGAAGGACATCCAGATAAGATTGCAGACCAGATTTCAGATGC
TATTTTAGATGCTATTTTGGCCAAGGATCCAGAGGCCCACGTGGCTGCAGAGACCTGCGTTTATACAGGCTCTGTTCATG
TTTTTGGAGAAATTTCAACAACAGCCTATGTGGATATCAATCGTGTGGTTCGTGATACCATTGCTGAGATTGGCTATACA
GAGGCAGAATACGGCTTTTCAGCAGAATCAGTGGGTGTTCATCCGTCTCTTGTTGAGCAGTCACCAGATATTGCTCAAGG
TGTTAATGAAGCACTTGAAGCACGTGAAGGACAAAGCGACGATTTCAATGTGATTGGTGCTGGAGATCAGGGCTTGATGT
TTGGTTTTGCAATTGATGAGACACCAGAACTAATGCCACTGCCGATTTCCTTATCTCATCAGCTTGTACGCCGTTTGGCG
ACACTGCGAAAATCAGGTGAGATCAGCTATTTGCGTCCAGACGCTAAATCACAGGTTACGGTCGAATATGATGAACACGA
TAAGCCTGTTCGTGTTGATACAGTTGTTATTTCAACCCAGCATGATCCAGAAGTAAGCAATGACCAGATTCGTCAAGATA
TGATTGAGCAGGTGATTAAGGCTGTTATTCCAGCTCACTATCTAGACGAGAAGACAAGATTCCTTATCAATCCGACTGGG
CGTTTTGTTATTGGTGGTCCTCAGGGAGATTCTGGCTTGACTGGTCGTAAGATCATTGTTGACACCTATGGTGGCTATGC
GCGTCATGGTGGTGGTGCCTTCTCTGGTAAAGACGCAACTAAGGTTGACCGCTCAGCCTCCTATGCAGCTCGTTATATTG
CCAAAAACCTTGTGGCAGCAGGGCTTGCAAGTAAAGCAGAGGTCCAATTAGCCTATGCTATCGGGGTTGCACAGCCAGTT
TCAGTTCGTGTGGATACCTTTGGGACATCAACAGTGTCAGAAAGTATTTTGGAAGCGGCTGTTCGTCAGGTCTTTGACCT
GCGCCCGGCAGGGATCATTAAGATGCTTGATCTCAAGCGTCCGATCTATAAGCAAACAGCAGCCTATGGGCATATGGGGC
GAACAGACATTGACCTGCCTTGGGAACACTTAGACAAGGTATCTCCATTGACAGAGGCGGTAGCAGCACTGTCAGAGGGT
GCTTAA

Upstream 100 bases:

>100_bases
ATCCTGTTATTTCAGTGGCTGGCTTGAGGTCTAAGGCTAGGAAAATCCTATCTAGCAGCGCTGCTAGTAGGTATCATTCG
TTTTAAGGGGGGACATGTCT

Downstream 100 bases:

>100_bases
GCAGATTGAGCGCCGGTTAGTTGATGCTTGACTTAAAGGTATAATAGCTCCTGAGCTAGTGGCTGTGCTGCTAGTTGGGA
GCTTTTTTAGTCCAGCAAAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETCVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYT
EAEYGFSAESVGVHPSLVEQSPDIAQGVNEALEAREGQSDDFNVIGAGDQGLMFGFAIDETPELMPLPISLSHQLVRRLA
TLRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEVSNDQIRQDMIEQVIKAVIPAHYLDEKTRFLINPTG
RFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSASYAARYIAKNLVAAGLASKAEVQLAYAIGVAQPV
SVRVDTFGTSTVSESILEAAVRQVFDLRPAGIIKMLDLKRPIYKQTAAYGHMGRTDIDLPWEHLDKVSPLTEAVAALSEG
A

Sequences:

>Translated_401_residues
MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETCVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYT
EAEYGFSAESVGVHPSLVEQSPDIAQGVNEALEAREGQSDDFNVIGAGDQGLMFGFAIDETPELMPLPISLSHQLVRRLA
TLRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEVSNDQIRQDMIEQVIKAVIPAHYLDEKTRFLINPTG
RFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSASYAARYIAKNLVAAGLASKAEVQLAYAIGVAQPV
SVRVDTFGTSTVSESILEAAVRQVFDLRPAGIIKMLDLKRPIYKQTAAYGHMGRTDIDLPWEHLDKVSPLTEAVAALSEG
A
>Mature_400_residues
SERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETCVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTE
AEYGFSAESVGVHPSLVEQSPDIAQGVNEALEAREGQSDDFNVIGAGDQGLMFGFAIDETPELMPLPISLSHQLVRRLAT
LRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEVSNDQIRQDMIEQVIKAVIPAHYLDEKTRFLINPTGR
FVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSASYAARYIAKNLVAAGLASKAEVQLAYAIGVAQPVS
VRVDTFGTSTVSESILEAAVRQVFDLRPAGIIKMLDLKRPIYKQTAAYGHMGRTDIDLPWEHLDKVSPLTEAVAALSEGA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=57.1052631578947, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=57.4803149606299, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1789311, Length=390, Percent_Identity=59.4871794871795, Blast_Score=467, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=58.005249343832, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17538494, Length=387, Percent_Identity=57.1059431524548, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=55.4987212276215, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17551082, Length=394, Percent_Identity=54.3147208121827, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565905, Length=386, Percent_Identity=52.8497409326425, Blast_Score=359, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=55.8208955223881, Blast_Score=349, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI25145635, Length=339, Percent_Identity=53.0973451327434, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI32565903, Length=309, Percent_Identity=55.9870550161812, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=99, Percent_Identity=52.5252525252525, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=55.0649350649351, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6320710, Length=392, Percent_Identity=53.5714285714286, Blast_Score=399, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=53.1578947368421, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=53.1578947368421, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=53.1578947368421, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=53.1578947368421, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=53.1578947368421, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=53.1578947368421, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=52.3684210526316, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=52.3684210526316, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580541, Length=382, Percent_Identity=50, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=48.8, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43341; Mature: 43210

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETCVYTGSVHVFGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEEECC
TAYVDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSPDIAQGVNEALEAREGQSD
EEEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCC
DFNVIGAGDQGLMFGFAIDETPELMPLPISLSHQLVRRLATLRKSGEISYLRPDAKSQVT
CCEEEECCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEE
VEYDEHDKPVRVDTVVISTQHDPEVSNDQIRQDMIEQVIKAVIPAHYLDEKTRFLINPTG
EEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCC
RFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSASYAARYIAKNLVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLASKAEVQLAYAIGVAQPVSVRVDTFGTSTVSESILEAAVRQVFDLRPAGIIKMLDLKR
CCCCCHHEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
PIYKQTAAYGHMGRTDIDLPWEHLDKVSPLTEAVAALSEGA
HHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAETCVYTGSVHVFGEIST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEEECC
TAYVDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSPDIAQGVNEALEAREGQSD
EEEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCC
DFNVIGAGDQGLMFGFAIDETPELMPLPISLSHQLVRRLATLRKSGEISYLRPDAKSQVT
CCEEEECCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEE
VEYDEHDKPVRVDTVVISTQHDPEVSNDQIRQDMIEQVIKAVIPAHYLDEKTRFLINPTG
EEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCC
RFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSASYAARYIAKNLVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLASKAEVQLAYAIGVAQPVSVRVDTFGTSTVSESILEAAVRQVFDLRPAGIIKMLDLKR
CCCCCHHEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
PIYKQTAAYGHMGRTDIDLPWEHLDKVSPLTEAVAALSEGA
HHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA